pCMV-SPORT6-slc26a2

LOCUS       pCMV-SPORT6-slc26a2               7466 bp DNA     circular SYN 02-OCT-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7466)
  TITLE     Epstein Lab Plasmids
REFERENCE   2  (bases 1 to 7466)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 7, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7466
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(5..27)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     polyA_signal    417..551
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(454..473)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     508..527
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      677..1132
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(764..783)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     974..995
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             1420..2280
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1638..1657)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     protein_bind    complement(2382..2415)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     rep_origin      2493..3081
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2982..3001
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3235..3252
                     /label=L4440
                     /note="L4440 vector, forward primer"
     enhancer        3541..3844
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3845..4048
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3999..4019
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4045..4069
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     4155..4177
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4174..4190
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4174..4190
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        4201..4219
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     4201..4218
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    4220..4244
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4509..6728
                     /codon_start=1
                     /label=SLC26A2-MICE
                     /translation="MSSENKEQHDLSPRDLPEEAFGFPSELPLEAQRRSGTDLRQSETG
                     HGRRAFRRIHMELREKPDTDIKQFVIRELQKSCQCSAAKVRDGAFDFFPVLRWLPKYDL
                     KKNILGDVMSGLIVGILLVPQSIAYSLLAGQEPIYGLYTSFFASIIYFLFGTSRHISVG
                     IFGILCLMIGEVVDRELHKACPDTDATSSSIAVFSSGCVVVNHTLDGLCDKSCYAIKIG
                     STVTFMAGVYQVAMGFFQVGFVSVYLSDALLSGFVTGASFTILTSQAKYLLGLSLPRSH
                     GVGSVITTWIHIFRNIRNTNICDLITSLLCLLVLVPSKELNEHFKDKLKAPIPVELIVV
                     VAATLASHFGKLNGNYNSSIAGHIPTGFMPPKAPDWSLIPNVAVDAIAISIIGFAITVS
                     LSEMFAKKHGYTVKANQEMYAIGFCNIIPSFFHCITTSAALAKTLVKESTGCQTQLSAI
                     VTALVLLLVLLVIAPLFYSLQKCVLGVITIVNLRGALLKFRDLPKMWRLSRMDTVIWFV
                     TMLSSALLSTEIGLLVGVCFSMFCVILRTQKPKNSLLGLEEESETFESISTYKNLRSKS
                     GIKVFRFIAPLYYINKECFKSALYKKALNPVLVKAAWKKAAKRKLKEEMVTFRGDPDEV
                     SMQLSHDPLEVHTIVIDCSAIQFLDTAGIHTLKEVRRDYEAVGIQVLLAQCNPSVRDSL
                     ARGEYCKKEEETLLFYSLSEAVAFAEDSQNQKGVCVVNGLSLSGD"
     CDS             5567..5578
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     protein_bind    complement(7407..7431)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(7432..7451)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7433..7451)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(join(7462..7466,1..13))
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(join(7462..7466,1..12))
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ccgtcgtttt acaacgtcgt gactgggaaa actgctagct tgggatcttt gtgaaggaac
       61 cttacttctg tggtgtgaca taattggaca aactacctac agagatttaa agctctaagg
      121 taaatataaa atttttaagt gtataatgtg ttaaactagc tgcatatgct tgctgcttga
      181 gagttttgct tactgagtat gatttatgaa aatattatac acaggagcta gtgattctaa
      241 ttgtttgtgt attttagatt cacagtccca aggctcattt caggcccctc agtcctcaca
      301 gtctgttcat gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa
      361 acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact
      421 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
      481 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
      541 atgtctggat cgatcctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
      601 attggctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
      661 ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
      721 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
      781 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
      841 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
      901 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
      961 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     1021 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     1081 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat ttcaggtggc
     1141 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     1201 atgtatccgc tcatgccagg tcttggactg gtgagaacgg cttgctcggc agcttcgatg
     1261 tgtgctggag ggagaataaa ggtctaagat gtgcgataga gggaagtcgc attgaattat
     1321 gtgctgtgta gggatcgctg gtatcaaata tgtgtgccca cccctggcat gagacaataa
     1381 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     1441 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     1501 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     1561 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     1621 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     1681 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     1741 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     1801 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     1861 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     1921 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     1981 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     2041 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     2101 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     2161 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     2221 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     2281 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     2341 aaaaggatct aggtgaagat cctttttgat aatctcatgc cataacttcg tataatgtat
     2401 gctatacgaa gttatggcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     2461 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     2521 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     2581 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc
     2641 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     2701 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     2761 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     2821 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     2881 gagcattgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     2941 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     3001 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     3061 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     3121 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     3181 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     3241 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     3301 ccgattcatt aatgcagagc ttgcaattcg cgcgtttttc aatattattg aagcatttat
     3361 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     3421 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
     3481 atgacattaa cctataaaaa taggcgtagt acgaggccct ttcactcatt agatgcatgt
     3541 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
     3601 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
     3661 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
     3721 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
     3781 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
     3841 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
     3901 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
     3961 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
     4021 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
     4081 ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc tccggactct
     4141 agcctaggcc gcggagcgga taacaatttc acacaggaaa cagctatgac cattaggcct
     4201 atttaggtga cactatagaa caagtttgta caaaaaagca ggctggtacc ggtccggaat
     4261 tcccgggata tcgtcgacga gctcactagt cggcggccgc tgatcctgca ctcaagggac
     4321 tcctctggag cctgtggact tggatctatt agcgcccccc tccccgcgcc ccgcctccct
     4381 ctctggcctt ttttggggga ggagctctcc gagatccgga gagttcccga gggtcacccg
     4441 ccgcactgtg ctcgcttttt cgtctcgcct tcacctggat ataatttgcg agcgaagctg
     4501 cccccaggat gtcttcagaa aataaagagc agcatgacct ctcaccgagg gacttacctg
     4561 aagaagcctt tggtttccca tctgagctcc ccctggaggc tcaaagaagg tcaggcactg
     4621 acttgaggca gtctgagacc ggtcatggcc ggagagcttt ccgtaggatc cacatggagc
     4681 tccgtgagaa accagacaca gacatcaaac agtttgtcat cagagagctc cagaagagtt
     4741 gccagtgcag cgcagccaaa gtcagagacg gggcttttga tttctttcct gttttgcggt
     4801 ggctcccaaa atatgacctg aagaaaaaca ttttaggtga tgtgatgtct ggcctgattg
     4861 tgggtatcct gttggtgccc cagtccattg cttactccct gctggctggc caggagccta
     4921 tatatggtct atatacatca ttttttgcca gcattattta tttcctgttt ggtacatccc
     4981 gccacatctc tgtgggcatt tttggaatac tgtgccttat gattggtgag gttgttgacc
     5041 gagaactaca taaagcctgc cctgacactg atgctacgtc atcttcgata gcagtgtttt
     5101 caagtggatg tgtggttgta aaccatacat tagacggact ctgtgataaa agctgctatg
     5161 caattaaaat tggcagcact gtgaccttca tggctggagt ttatcaggta gcaatgggct
     5221 tctttcaagt gggctttgtc tctgtctacc tctccgacgc cttgctgagc gggtttgtca
     5281 ctggcgcctc cttcaccatc ctcacgtctc aggccaagta cctccttggg ctgagccttc
     5341 ctcggagtca tggtgtcggc tcagtcatta ctacctggat ccacatcttc agaaacatcc
     5401 gtaacaccaa catctgtgat ctcatcacca gccttttatg tcttctggtc ctcgtgccat
     5461 ccaaagaact caatgagcac ttcaaggaca agctcaaggc tccaattccg gtcgagctca
     5521 ttgtcgttgt ggcggccaca ttggcttctc attttggaaa actaaatggg aattacaatt
     5581 ccagtattgc tggacacatt cccactgggt ttatgccacc caaagcacca gactggagcc
     5641 tcattcctaa tgtggctgta gatgcaatag ctatttctat cattggtttt gctatcactg
     5701 tatcactttc tgagatgttt gccaagaaac atggctacac ggtcaaagca aaccaagaaa
     5761 tgtatgccat tggcttttgc aatatcatac cttccttctt ccactgcata actactagtg
     5821 cagctcttgc aaagacattg gttaaagagt caacaggctg ccagacgcag ctgtcagcta
     5881 tagtgacagc ccttgtcctt ttgttggtcc ttctggtaat agctccttta ttctattccc
     5941 ttcaaaaatg tgtccttggt gtgatcacta ttgtaaatct ccggggcgca cttctgaaat
     6001 ttagagacct gccaaagatg tggaggctaa gcagaatgga tacagttatc tggtttgtga
     6061 ctatgctgtc ctccgctctg ttaagcactg aaataggcct gctggttggg gtttgttttt
     6121 caatgttctg tgtgatcctc cgtactcaga agccaaagaa ttcactgctt ggtttagaag
     6181 aagaatctga aacctttgaa tccatttcca cttacaagaa ccttcggagc aagtccggca
     6241 tcaaggtttt ccgcttcata gcccctctct actacataaa caaagagtgc tttaaatcag
     6301 ctttgtacaa gaaagctctt aacccagtct tggtaaaggc agcttggaaa aaggcagcaa
     6361 agagaaaact caaagaagaa atggtgacct ttcgtgggga cccggatgaa gtttcaatgc
     6421 agctttccca tgatcccttg gaggtgcaca ctatcgtgat cgactgtagt gccatacagt
     6481 ttttagacac agcaggaatc cacacactga aagaagttcg ccgggattat gaagccgttg
     6541 ggatccaggt tttactggcg cagtgcaatc cttctgtgag ggattccttg gccagaggag
     6601 aatattgcaa aaaggaagag gaaacccttc tcttctacag tctgtctgaa gcagtagcct
     6661 ttgcagaaga ctctcagaat cagaaagggg tgtgtgttgt caacggtctg agtctctctg
     6721 gtgactgagg gggtagttcc tcctggaagg gagagagatc acctcttgct tgagaacggg
     6781 gaactggagg catgtttaag cctttcatcc cagtatcatt tttttgtggc aaagacagct
     6841 gattgctaca gcacaggctc ccttgtgtaa ttatttgctt aactgatgtc aataacatct
     6901 tgcagttatt aattgctggg acggaaaacc ggattgtcag taggtaaaac acagaggttg
     6961 gccaaaatga aataacctct agcattagaa acacatgttn ttaatgaggt cagcgccagg
     7021 atcatatggg gataagccca ggacacagag ttgtgtcaaa cttgtntcag gaataaaaat
     7081 attagtctcg atcctttgat accgtggtat taaatatgac attgtcagcc tgtagctgat
     7141 cttgccccta actgtgaatt gtcccagcat gacctaaaaa gctgcgtgtg tttccttaca
     7201 ggtgcctttc attatctcaa gccagatatt gcataagtga actgtccact tggagccctg
     7261 tttccggcct ccacagacta gacatgaaga atnttntcag aaacaaaaca ataaaaaaaa
     7321 tttaaaaaca aacaaaaaac cttgaccgta agacatgaat tagcggccgc tctagagtat
     7381 ccctcgaggg gcccaagctt acgcgtaccc agctttcttg tacaaagtgg tccctatagt
     7441 gagtcgtatt ataagctagg cactgg
//