pCuO-mGreenLantern-iPAK4-EF1a-CymR

LOCUS       pCuO-mGreenLantern-iPAK4-EF1a-CymR             8006 bp DNA     circular SYN 23-APR-2024
DEFINITION  Express FlicR1 preferentially in neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8006)
  AUTHORS   Abdelfattah AS, Farhi SL, Zhao Y, Brinks D, Zou P, Ruangkittisakul 
            A, Platisa J, Pieribone VA, Ballanyi K, Cohen AE, Campbell RE
  TITLE     A Bright and Fast Red Fluorescent Protein Voltage Indicator That 
            Reports Neuronal Activity in Organotypic Brain Slices.
  JOURNAL   J Neurosci. 2016 Feb 24;36(8):2458-72. doi: 
            10.1523/JNEUROSCI.3484-15.2016.
  PUBMED    26911693
REFERENCE   2  (bases 1 to 8006)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 13, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1523/JNEUROSCI.3484-15"; journalName: "J Neurosci"; date: 
            "2016-02-24- 24"; volume: "36"; issue: "8"; pages: "2458-72"
FEATURES             Location/Qualifiers
     source          1..8006
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          2547..3623
                     /mol_type="genomic DNA"
                     /organism="."
     protein_bind    229..250
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        265..295
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    303..319
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     308..330
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     327..343
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     327..343
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   381..510
                     /label=AAV2 ITR
     misc_feature    566..1808
                     /label=CMV+CuO
     enhancer        630..933
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(639..656)
                     /label=PAD-Track-F
     misc_feature    689..696
                     /label=CRE
     misc_feature    742..749
                     /label=CRE
     primer_bind     complement(748..767)
                     /label=pEP-330x-R
     misc_feature    825..832
                     /label=CRE
     primer_bind     complement(915..934)
                     /label=PSI-R
     primer_bind     981..1000
                     /label=PSIH-F
     misc_feature    1011..1018
                     /label=CRE
     primer_bind     1116..1135
                     /label=PIRES2-EGFP.P5'
     primer_bind     1117..1136
                     /label=pLVX-F
     primer_bind     1228..1251
                     /label=PQC-seq-F
     primer_bind     1236..1258
                     /label=CMV
     primer_bind     1245..1264
                     /label=PTRE2HYG-F
     primer_bind     1737..1759
                     /label=pTT5-F
     primer_bind     1758..1777
                     /label=T7（17BASE）
     primer_bind     1767..1786
                     /label=PMT2-F
     primer_bind     complement(1792..1843)
                     /label=NheI-CuO-R
     primer_bind     1815..1843
                     /label=NheI-mGL-F
     misc_feature    1818..1823
                     /label=MCS
     regulatory      1824..1833
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1830..2546
                     /codon_start=1
                     /label=mGreenLantern
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLGYGVACFARYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIVLKGIDFKEDGNILGHKLEYNFNSHKVYITADKQKNGIK
                     ANFKTRHNVEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLK
                     ERVTAAGITHDMDELYK"
     primer_bind     complement(2531..2572)
                     /label=INKA-mGreenLantern-R
     CDS             2559..2672
                     /codon_start=1
                     /label=INKA-box
                     /translation="EAEDWTAALLNRGRSRQPLVLGDNCFADLVHNWMELPE"
     CDS             2679..3620
                     /codon_start=1
                     /label=Pak4cat
                     /translation="PAAPAVPGPPGPRSPQREPQRVSHEQFRAALQLVVDPGDPRSYLD
                     NFIKIGEGSTGIVCIATVRSSGKLVAVKKMDLRKQQRRELLFNEVVIMRDYQHENVVEM
                     YNSYLVGDELWVVMEFLEGGALTDIVTHTRMNEEQIAAVCLAVLQALSVLHAQGVIHRD
                     IKSDSILLTHDGRVKLSDFGFCAQVSKEVPRRKSLVGTPYWMAPELISRLPYGPEVDIW
                     SLGIMVIEMVDGEPPYFNEPPLKAMKMIRDNLPPRLKNLHKVSPSLKGFLDRLLVRDPA
                     QRATAAELLKHPFLAKAGPPASIVPLMRQNRTR"
     primer_bind     complement(2772..2790)
                     /label=PAK4-R
     primer_bind     complement(3602..3648)
                     /label=XbaI-PAK-R
     primer_bind     3631..3656
                     /label=EF1a-F
     promoter        3666..3877
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     primer_bind     complement(3720..3742)
                     /label=pCDH1-R
     primer_bind     complement(3722..3741)
                     /label=PCDH-R(EF1a-R)
     primer_bind     complement(3857..3905)
                     /label=BglII-EF1a-R
     primer_bind     3888..3938
                     /label=BglII-CymR-F
     regulatory      3905..3910
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3911..4519
                     /codon_start=1
                     /gene="cymR from Pseudomonas putida"
                     /product="cumate repressor (Mullick et al., 2006)"
                     /label=CymR
                     /translation="MSPKRRTQAERAMETQGKLIAAALGVLREKGYAGFRIADVPGAAG
                     VSRGAQSHHFPTKLELLLATFEWLYEQITERSRARLAKLKPEDDVIQQMLDDAAEFFLD
                     DDFSISLDLIVAADRDPALREGIQRTVERNRFVVEDMWLGVLVSRGLSRDDAEDILWLI
                     FNSVRGLAVRSLWQKDKERFERVRNSTLEIARERYAKFKR"
     primer_bind     complement(4499..4542)
                     /label=NotI-CymR-R
     primer_bind     4513..4568
                     /label=WPRE-F
     misc_feature    4531..5119
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(5002..5013)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5101..5151)
                     /label=WPRE-R
     polyA_signal    5153..5274
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5164..5183)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5218..5237
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(5256..5274)
                     /label=SV40-polyA
     repeat_region   5436..5565
                     /label=AAV2 ITR
     primer_bind     complement(5584..5601)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5584..5600)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5593..5615)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5742..6197
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5829..5848)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6039..6060
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6223..6327
                     /gene="bla"
                     /label=AmpR promoter
     CDS             6328..7188
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6546..6565)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7359..7947
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7848..7867
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
       61 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
      121 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
      181 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
      241 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
      301 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccagatt
      361 taattaaggc cttaattagg ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc
      421 ccgggcgtcg ggcgaccttt ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg
      481 gagtggccaa ctccatcact aggggttcct tgtagttaat gattaacccg ccatgctact
      541 tatctacgta gccatgctct aggacagact agttattaat agtaatcaat tacggggtca
      601 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
      661 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
      721 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
      781 ttggcagtac atcaagtgta tcatatgcca agtccgcccc ctattgacgt caatgacggt
      841 aaatggcccg cctggcatta tgcccagtac atgaccttac gggactttcc tacttggcag
      901 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacaccaat
      961 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
     1021 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa tacccccgcc
     1081 ccgttgacgc aaatgggcaa gcttgccggg tcgaggtagg cgtgtacggt gggaggccta
     1141 tataagcaac cggtataatc aaacagacca gattgtctgt ttgttaccgg tgtttagtga
     1201 accgggcgcg cctcatatcg cctggagacg ccatccacgc tgttttgacc tccatagaag
     1261 acaccgggac cgatccagcc tccgcggtca ctctcttccg catcgctgtc tgcgagggcc
     1321 agctgttggg ctcgcggttg aggacaaact cttcgcggtc tttccagtac tcttggatcg
     1381 gaaacccgtc ggcctccgaa cggtactccg ccaccgaggg acctgagcga gtccgcatcg
     1441 accggatcgg aaaacctctc gagaaaggcg tctaaccagt cacagtcgca aggtaggctg
     1501 agcaccgtgg cgggcggcag cgggtggcgg tcggggttgt ttctggcgga ggtgctgctg
     1561 atgatgtaat taaagtaggc ggtcttgaga cggcggatgg tcgaggtgag gtgtggcagg
     1621 cttgagatcc agctgttggg gtgagtactc cctctcaaaa gcgggcatta cttctgcgct
     1681 aagattgtca gtttccaaaa acgaggagga tttgatattc acctggcccg atctggccat
     1741 acacttgagt gacaatgaca tccactttgc ctttctctcc acaggtgtcc actcccaggt
     1801 ccaagtttgg tctagcagct agcgccacca tggtgagcaa gggcgaggag ctgttcaccg
     1861 gcgtggtgcc tatcctggtg gagctggacg gcgacgtgaa cggccacaag ttcagcgtga
     1921 ggggcgaggg cgagggcgac gccaccaacg gcaagctgac cctgaagttc atctgcacca
     1981 ccggcaagct gcctgtgcct tggcctaccc tggtgaccac cctgggctac ggcgtggcct
     2041 gcttcgccag gtaccctgac cacatgaagc agcacgactt cttcaagagc gccatgcctg
     2101 agggctacgt gcaggagagg accatcagct tcaaggacga cggcacctac aagaccaggg
     2161 ccgaggtgaa gttcgagggc gacaccctgg tgaacaggat cgtgctgaag ggcatcgact
     2221 tcaaggagga cggcaacatc ctgggccaca agctggagta caacttcaac agccacaagg
     2281 tgtacatcac cgccgacaag cagaagaacg gcatcaaggc caacttcaag accaggcaca
     2341 acgtggagga cggcggcgtg cagctggccg accactacca gcagaacacc cctatcggcg
     2401 acggccctgt gctgctgcct gacaaccact acctgagcca ccagagcaag ctgagcaagg
     2461 accctaacga gaagagggac cacatggtgc tgaaggagag ggtgaccgcc gccggcatca
     2521 cccacgacat ggacgagctg tacaagtcag gtggatccga agcagaggac tggacagcag
     2581 ccctactgaa caggggtcgc agtcgccagc ccctggtact aggggacaat tgctttgctg
     2641 acttggtgca caactggatg gagctgcctg aggaattccc cgccgcccct gctgttcctg
     2701 ggccccctgg cccccgctca ccacagcggg agccacagcg agtatcccat gagcagttcc
     2761 gggctgccct gcagctggtg gtggacccag gcgacccccg ctcctacctg gacaacttca
     2821 tcaagattgg cgagggctcc acgggcatcg tgtgcatcgc caccgtgcgc agctcgggca
     2881 agctggtggc cgtcaagaag atggacctgc gcaagcagca gaggcgcgag ctgctcttca
     2941 acgaggtggt aatcatgagg gactaccagc acgagaatgt ggtggagatg tacaacagct
     3001 acctggtggg ggacgagctc tgggtggtca tggagttcct ggaaggaggc gccctcaccg
     3061 acatcgtcac ccacaccagg atgaacgagg agcagatcgc agccgtgtgc cttgcagtgc
     3121 tgcaggccct gtcggtgctc cacgcccagg gcgtcatcca ccgggacatc aagagcgact
     3181 cgatcctgct gacccatgat ggcagggtga agctgtcaga ctttgggttc tgcgcccagg
     3241 tgagcaagga agtgccccga aggaagtcgc tggtcggcac gccctactgg atggccccag
     3301 agctcatctc ccgccttccc tacgggccag aggtagacat ctggtcgctg gggataatgg
     3361 tgattgagat ggtggacgga gagcccccct acttcaacga gccacccctc aaagccatga
     3421 agatgattcg ggacaacctg ccaccccgac tgaagaacct gcacaaggtg tcgccatccc
     3481 tgaagggctt cctggaccgc ctgctggtgc gagaccctgc ccagcgggcc acggcagccg
     3541 agctgctgaa gcacccattc ctggccaagg cagggccgcc tgccagcatc gtgcccctca
     3601 tgcgccagaa ccgcaccaga tgaatctaga atttaaaatc tgcgatcgct ccggtgcccg
     3661 tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa
     3721 ttgaacgggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg
     3781 gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa
     3841 cgttcttttt cgcaacgggt ttgccgccag aacacagcaa gctgtgaccg gcgcctacag
     3901 atctgccgcc atgagtccaa agagaagaac acaggcagag cgcgcaatgg agacccaggg
     3961 caagttgatt gcagcggccc tgggggtttt acgggaaaaa ggttacgcgg gattccggat
     4021 cgcagatgtg cccggtgctg caggtgtctc gagaggagcg cagagccatc atttcccgac
     4081 aaagcttgag cttctgcttg ccacttttga atggctttac gaacagatca ccgaacgcag
     4141 tcgggctcga ttagcgaaat tgaagccaga ggatgacgtc atccagcaaa tgctggacga
     4201 cgccgccgaa tttttcctcg acgatgactt ctctatcagc cttgatttga ttgtggctgc
     4261 cgaccgggat ccagcgttac gcgagggtat tcagcgcacg gtagagagga atcggtttgt
     4321 cgtcgaggat atgtggcttg gtgttctggt gagccgtggt ctttcgcgtg atgatgcaga
     4381 agatatcctt tggttgatat tcaattcggt gcgtgggctt gctgttcgta gcctatggca
     4441 gaaggacaaa gaacgctttg agcgtgtcag gaactcgaca ctcgaaattg cgcgagagcg
     4501 gtacgcgaaa ttcaagcgct gagcggccgc aatcaacctc tggattacaa aatttgtgaa
     4561 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     4621 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     4681 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     4741 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     4801 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     4861 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     4921 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
     4981 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     5041 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     5101 ctttgggccg cctccccgcg tcgacctcga cggccgcttc gagcagacat gataagatac
     5161 attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa
     5221 atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac
     5281 aacaattgca ttcattttat gtttcaggtt cagggggaga tgtgggaggt tttttaaagc
     5341 aagtaaaacc tctacaaatg tggtaaaatc gataaggatc ttcctagagc atggctacgt
     5401 agataagtag catggcgggt taatcattaa ctacaaggaa cccctagtga tggagttggc
     5461 cactccctct ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg
     5521 cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg cgcagcctta attaacctaa
     5581 ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
     5641 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
     5701 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
     5761 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     5821 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     5881 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     5941 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     6001 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     6061 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     6121 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     6181 attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     6241 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     6301 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     6361 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     6421 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     6481 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     6541 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     6601 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     6661 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     6721 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     6781 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     6841 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     6901 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     6961 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     7021 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     7081 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     7141 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     7201 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     7261 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     7321 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     7381 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     7441 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     7501 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     7561 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     7621 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     7681 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     7741 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     7801 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     7861 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     7921 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     7981 gccttttgct ggccttttgc tcacat
//