pAAV-cFos1-mScarlet3-iPAK4

LOCUS       pAAV-cFos1-mScarlet3-iPAK4             7386 bp DNA     circular SYN 02-NOV-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7386)
  AUTHORS   Kiryl Piatkevich
  TITLE     Direct Submission
  JOURNAL   Exported Jul 16, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7386
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          311..1987
                     /lab_host="."
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          2689..3765
                     /mol_type="genomic DNA"
                     /organism="."
     misc_feature    1..130
                     /label=AAV2 ITR
     promoter        311..1987
                     /label=putative_fos
                     /note="/created_by=xiuyuanli
                     https://www.ncbi.nlm.nih.gov/nucleotide/V00727.1?report=gen
                     bank&log$=nuclalign&blast_rank=3&RID=55DC32WT016: 1-1618"
     promoter        607..924
                     /label=putative_fos_from34856
                     /note="/created_by=xiuyuanli"
                     /note="/modified_by=xiuyuanli"
     misc_feature    1072..1079
                     /label=Fos oncogene start
     CDS             2002..2688
                     /codon_start=1
                     /label=mScarlet3
                     /translation="MDSTEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LRVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYWKQSFPEGFKWERVMNFEDGGA
                     VSVAQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDVVLKGDIKM
                     ALRLKDGGRYLADFKTTYRAKKPVQMPGAFNIDRKLDITSHNEDYTVVEQYERSVARHS
                     TGGSGGS"
     regulatory      2002..2005
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(2132..2150)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2400..2419
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(2668..2717)
                     /label=iBox-mScarlet3-R
     primer_bind     2689..2714
                     /label=GFP-iPAK4-F
     primer_bind     complement(2689..2714)
                     /label=XFP-iPAK4-R
     CDS             2701..2814
                     /codon_start=1
                     /label=INKA-box
                     /translation="EAEDWTAALLNRGRSRQPLVLGDNCFADLVHNWMELPE"
     CDS             2821..3762
                     /codon_start=1
                     /label=Pak4cat
                     /translation="PAAPAVPGPPGPRSPQREPQRVSHEQFRAALQLVVDPGDPRSYLD
                     NFIKIGEGSTGIVCIATVRSSGKLVAVKKMDLRKQQRRELLFNEVVIMRDYQHENVVEM
                     YNSYLVGDELWVVMEFLEGGALTDIVTHTRMNEEQIAAVCLAVLQALSVLHAQGVIHRD
                     IKSDSILLTHDGRVKLSDFGFCAQVSKEVPRRKSLVGTPYWMAPELISRLPYGPEVDIW
                     SLGIMVIEMVDGEPPYFNEPPLKAMKMIRDNLPPRLKNLHKVSPSLKGFLDRLLVRDPA
                     QRATAAELLKHPFLAKAGPPASIVPLMRQNRTR"
     primer_bind     complement(2914..2932)
                     /label=PAK4-R
     misc_feature    3784..4372
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(4255..4266)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4390..4597
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   4649..4789
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      4864..5319
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5601..5705
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5706..6566
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6737..7325
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgttgcaaa gatggataaa gttttaaaca gagaggaatc
      181 tttgcagcta atggaccttc taggtcttga aaggagtggg aattggctcc ggtgcccgtc
      241 agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcagca
      301 aattaattaa aagctttcct ttaggaacag aggcttcgag cctttaaggc tgcgtacttg
      361 cttctcctaa taccagagac tcaaaaaaaa aaaaaaagtt ccagattgct ggacaatgac
      421 ccgggtctca tcccttgacc ctgggaaccg ggtccacatt gaatcaggtg cgaatgttcg
      481 ctcgccttct ctgcctttcc cgcctcccct cccccggccg cggccccggt tccccccctg
      541 cgctgcaccc tcagagttgg ctgcagccgg cgagctgttc ccgtcaatcc ctccctcctt
      601 tacacaggat gtccatatta ggacatctgc gtcagcaggt ttccacggcc ggtccctgtt
      661 gttctggggg ggggaccatc tccgaaatcc tacacgcgga aggtctagga gaccccctaa
      721 gatcccaaat gtgaacactc ataggtgaaa gatgtatgcc aagacggggg ttgaaagcct
      781 ggggcgtaga gttgacgaca gagcgcccgc agagggcctt ggggcgcgct tcccccccct
      841 tccagttccg cccagtgacg taggaagtcc atccattcac agcgcttcta taaaggcgcc
      901 agctgaggcg cctactactc caaccgcgac tgcagcgagc aactgagaag actggataga
      961 gccggcggtt ccgcgaacga gcagtgaccg cgctcccacc cagctctgct ctgcagctcc
     1021 caccagtgtc tacccctgga ccccttgccg ggctttcccc aaacttcgac catgatgttc
     1081 tcgggtttca acgccgacta cgaggcgtca tcctcccgct gcagtagcgc ctccccggcc
     1141 ggggacagcc tttcctacta ccattcccca gccgactcct tctccagcat gggctctcct
     1201 gtcaacacac aggtgagttt ggctttgtgt agccgccagg tccgcgctga gggtcgccgt
     1261 ggaggagaca ctggggtgtg actcgcaggg gcgggggggt cttccttttt cgctctggag
     1321 ggagactggc gcggtcagag cagccttagc ctgggaaccc aggacttgtc tgagcgcgtg
     1381 cacacttgtc atagtaagac ttagtgaccc cttcccgcgc ggcaggttta ttctgagtgg
     1441 cctgcctgca ttcttctctc ggccgacttg tttctgagat cagccggggc caacaagtct
     1501 cgagcaaaga gtcgctaact agagtttggg aggcggcaaa ccgcggcaat cccccctccc
     1561 ggggcagcct ggagcaggga ggagggngga gggaggaggg tgctgcgggc gggtgtgtaa
     1621 ggcagtttca ttgataaaaa gcgagttcat tctggagact ccggagcagc gcctgcgtca
     1681 gcgcagacgt cagggatatt tataacaaac cccctttcga gcgagtgatg ccgaagggat
     1741 aacgggaacg cagcagtagg atggagggga aaggctgcgc tgcggaattc aagggaggat
     1801 attgggagag cttttatctc cgatgaggtg catacaggaa gacataagca gtctctgacc
     1861 ggaatgcttc tctctccctg cttcatgcga cactagggcc acttgctcca cctgtgtctg
     1921 gaacctcctc gctcacctcc gctttcctct ttttgttttg tttcaggact tttgcgcaga
     1981 tctctccgag gatccgccac catggatagc accgaggcag tgatcaagga gttcatgcgg
     2041 ttcaaggtgc acatggaggg ctccatgaac ggccacgagt tcgagatcga gggcgagggc
     2101 gagggccgcc cctacgaggg cacccagacc gccaagctga gggtgaccaa gggtggcccc
     2161 ctgcccttct cctgggacat cctgtcccct cagttcatgt acggctccag ggccttcacg
     2221 aagcaccccg ccgacatccc cgactactgg aagcagtcct tccccgaggg cttcaagtgg
     2281 gagcgcgtga tgaacttcga ggacggcggc gccgtgtccg tggcccagga cacctccctg
     2341 gaggacggca ccctgatcta caaggtgaag ctccgcggca ccaacttccc tcctgacggc
     2401 cccgtaatgc agaagaagac aatgggctgg gaagcatcca ccgagcggtt gtaccccgag
     2461 gacgtcgtgc tgaagggcga cattaagatg gccctgcgcc tgaaggacgg cggccgctac
     2521 ctggcggact tcaagaccac ctacagggcc aagaagcccg tgcagatgcc cggcgccttc
     2581 aacatcgacc gcaagttgga catcacatcc cacaacgagg actacaccgt ggtggaacag
     2641 tacgaacgct ccgtggcccg ccactccacc ggcggctccg gtggctcctc aggtggatcc
     2701 gaagcagagg actggacagc agccctactg aacaggggtc gcagtcgcca gcccctggta
     2761 ctaggggaca attgctttgc tgacttggtg cacaactgga tggagctgcc tgaggaattc
     2821 cccgccgccc ctgctgttcc tgggccccct ggcccccgct caccacagcg ggagccacag
     2881 cgagtatccc atgagcagtt ccgggctgcc ctgcagctgg tggtggaccc aggcgacccc
     2941 cgctcctacc tggacaactt catcaagatt ggcgagggct ccacgggcat cgtgtgcatc
     3001 gccaccgtgc gcagctcggg caagctggtg gccgtcaaga agatggacct gcgcaagcag
     3061 cagaggcgcg agctgctctt caacgaggtg gtaatcatga gggactacca gcacgagaat
     3121 gtggtggaga tgtacaacag ctacctggtg ggggacgagc tctgggtggt catggagttc
     3181 ctggaaggag gcgccctcac cgacatcgtc acccacacca ggatgaacga ggagcagatc
     3241 gcagccgtgt gccttgcagt gctgcaggcc ctgtcggtgc tccacgccca gggcgtcatc
     3301 caccgggaca tcaagagcga ctcgatcctg ctgacccatg atggcagggt gaagctgtca
     3361 gactttgggt tctgcgccca ggtgagcaag gaagtgcccc gaaggaagtc gctggtcggc
     3421 acgccctact ggatggcccc agagctcatc tcccgccttc cctacgggcc agaggtagac
     3481 atctggtcgc tggggataat ggtgattgag atggtggacg gagagccccc ctacttcaac
     3541 gagccacccc tcaaagccat gaagatgatt cgggacaacc tgccaccccg actgaagaac
     3601 ctgcacaagg tgtcgccatc cctgaagggc ttcctggacc gcctgctggt gcgagaccct
     3661 gcccagcggg ccacggcagc cgagctgctg aagcacccat tcctggccaa ggcagggccg
     3721 cctgccagca tcgtgcccct catgcgccag aaccgcacca gatgacgtac aaagcttatc
     3781 gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     3841 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     3901 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     3961 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     4021 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     4081 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     4141 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg
     4201 ctcgcctatg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     4261 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     4321 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcgatac
     4381 cggctcgagc tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
     4441 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
     4501 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
     4561 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtctcgagc tgattttgta
     4621 ggtaaccacg tgcggaccga gcggccgcag gaacccctag tgatggagtt ggccactccc
     4681 tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc
     4741 tttgcccggg cggcctcagt gagcgagcga gcgcgcagct gcctgcaggg gcgcctgatg
     4801 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatacgtca aagcaaccat
     4861 agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     4921 ccgctacact tgccagcgcc ttagcgcccg ctcctttcgc tttcttccct tcctttctcg
     4981 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     5041 ttagtgcttt acggcacctc gaccccaaaa aacttgattt gggtgatggt tcacgtagtg
     5101 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     5161 gtggactctt gttccaaact ggaacaacac tcaactctat ctcgggctat tcttttgatt
     5221 tataagggat tttgccgatt tcggtctatt ggttaaaaaa tgagctgatt taacaaaaat
     5281 ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt atggtgcact ctcagtacaa
     5341 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc
     5401 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
     5461 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg
     5521 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
     5581 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     5641 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     5701 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     5761 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     5821 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     5881 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     5941 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     6001 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     6061 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     6121 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     6181 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     6241 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     6301 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     6361 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     6421 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     6481 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     6541 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     6601 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     6661 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     6721 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     6781 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     6841 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     6901 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     6961 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     7021 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     7081 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     7141 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     7201 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     7261 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     7321 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     7381 acatgt
//