pAAV-AscI-CAG-NES-NMT

LOCUS       pAAV-AscI-CAG-NES-NMT           5630 bp DNA     circular SYN 24-JUN-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pAAV-AscI-CAG-NES-NMT
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5630)
  AUTHORS   1
  TITLE     Direct Submission
  JOURNAL   Exported Jul 16, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
COMMENT     ORIGDB|GenBank
FEATURES             Location/Qualifiers
     source          1..5630
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    52..992
                     /label=ColE1
                     /note="ColE1"
     CDS             complement(993..1853)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(1854..1958)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1984..2439)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     polyA_signal    2802..2923
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    2966..3554
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             3072..3083
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     misc_feature    3413..3418
                     /label=SDS
     misc_feature    complement(3597..4418)
                     /label=NMT
                     /note="/vntifkey=21"
     misc_feature    4397..4402
                     /label=SDS
     misc_feature    4425..4460
                     /label=NES
                     /note="/vntifkey=21"
     CDS             complement(4425..4460)
                     /codon_start=1
                     /label=NES
                     /note="/vntifkey=21"
                     /translation="LQLPPLERLTLS"
     misc_feature    4446..4451
                     /label=SDS
     primer_bind     4485..4511
                     /label=172-R
     misc_feature    complement(4884..5161)
                     /label=chicken b-actin promoter
                     /label=chicken\b-actin\promoter
                     /note="/vntifkey=21"
     promoter        complement(4884..5161)
                     /label=chicken beta-actin promoter
     misc_feature    complement(5168..5411)
                     /label=CMV enhancer
                     /label=CMV\enhancer
                     /note="/vntifkey=21"
     misc_feature    5412..5426
                     /label=AscI-OxE insert
                     /label=AscI-OxE\insert
                     /note="/vntifkey=21"
ORIGIN
        1 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
       61 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
      121 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
      181 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
      241 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
      301 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
      361 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
      421 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
      481 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
      541 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
      601 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
      661 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
      721 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
      781 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
      841 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
      901 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
      961 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     1021 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     1081 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     1141 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     1201 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     1261 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     1321 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     1381 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     1441 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     1501 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     1561 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     1621 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     1681 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     1741 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     1801 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     1861 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     1921 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     1981 cctaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct
     2041 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg
     2101 agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact
     2161 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac
     2221 cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga
     2281 gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga
     2341 aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca
     2401 ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc ccattcgcca ttcaggctgc
     2461 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag ctgcgcgctc
     2521 gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt ggtcgcccgg
     2581 cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact aggggttcct
     2641 tgtagttaat gattaacccg ccatgctact tatctacgta gccatgctct aggaagatcc
     2701 ttatcgattt taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacatctcc
     2761 ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt
     2821 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     2881 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgctcgaagc
     2941 ggccgcccgg gtcgacggta tcgatgcggg gaggcggccc aaagggagat ccgactcgtc
     3001 tgagggcgaa ggcgaagacg cggaagaggc cgcagagccg gcagcaggcc gcgggaagga
     3061 aggtccgctg gattgagggc cgaagggacg tagcagaagg acgtcccgcg cagaatccag
     3121 gtggcaacac aggcgagcag ccaaggaaag gacgatgatt tccccgacaa caccacggaa
     3181 ttgtcagtgc ccaacagccg agcccctgtc cagcagcggg caaggcaggc ggcgatgagt
     3241 tccgccgtgg caatagggag ggggaaagcg aaagtcccgg aaaggagctg acaggtggtg
     3301 gcaatgcccc aaccagtggg ggttgcgtca gcaaacacag tgcacaccac gccacgttgc
     3361 ctgacaacgg gccacaactc ctcataaaga gacagcaacc aggatttata caaggaggag
     3421 aaaatgaaag ccatacggga agcaatagca tgatacaaag gcattaaagc agcgtatcca
     3481 catagcgtaa aaggagcaac atagttaaga ataccagtca atctttcaca aattttgtaa
     3541 tccagaggtt gattatcgat aagcttctac ttggaagaaa tgacacgaga gaattcctac
     3601 ttatacagct cgtccatgcc catcacgtcc gtaaaggcct tttgccactc tttaaaattc
     3661 agttcggtct tagaatgttt cagctctgtt ttccgaaaga catacatcgg ctgatttttg
     3721 aggtaatttg cggccattgg cttggcgaat gtgtatgtgg tgcgagcggt agaccggtag
     3781 cgtttgccgt tgtcggtgat aaaagcccac ttaaatgtgg agacgatagt cttatcattg
     3841 gggcaagtct tcttggatac gcaccagtca gcactaacaa gggcgcagca gctgcacttg
     3901 tccgaagcct ctttgcagat gcagccctgg gagcacttca cacagcccac ggggcagcag
     3961 gaacagctgc ttttaagagt atcggctgtc agggaatttg tcatcacagg gccgtcagct
     4021 gggaacccag tgccctccac ctgagcttcc cctttgatat gtgatcctcc gtaggtgtat
     4081 ctgtaattca cggtcagtga ggcaccgtcc tcaaactgca tagtgcggtg cacttggtat
     4141 ccggacccgt cgaccattgc agcctgaaat ggtgacatcc cgtctggata gggaagatac
     4201 tggtgaaacc cgtacccaat gtgcggcacc aggatccagg gactgaactg cagatcgccc
     4261 atggtggact tcaggttcag ctcctcatag ccgtcgtttg gattaccggt gccttggcct
     4321 accatgtcga aatcgatgcc atttatggag ccaaaaatat gcagttcgtg ggtagcaggc
     4381 agactagcca tgttttcctc ctctcccttt gagaccatag atctcgaaag agtaagacgt
     4441 tcaagaggag gaagttgaag catggtgtcg gatccaattc tttgccaaaa tgatgagaca
     4501 gcacaataac cagcacgttg cccaggagct gtaggaaaaa gaagaaggca tgaacatggt
     4561 tagcagaggc tctagagccg ccggtcacac gccagaagcc gaaccccgcc ctgccccgtc
     4621 ccccccgaag gcagccgtcc ccccgcggac agccccgagc cgctcccccc gcacaaaggg
     4681 ccctcccgga gcccctcaag gctttcacgc agccacagaa aagaaacaag ccgtcattaa
     4741 accaagcgct aattacagcc cggaggagaa gggccgtccc gcccgctcac ctgtgggagt
     4801 aacgcggtca gtcagagccg gggcgggcgg cgcgaggcgg cggcggagcg gggcacgggg
     4861 cgaaggcagc gcgcagcgac tcccgcccgc cgcgcgcttc gctttttata gggccgccgc
     4921 cgccgccgcc tcgccataaa aggaaacttt cggagcgcgc cgctctgatt ggctgccgcc
     4981 gcacctctcc gcctcgcccc gccccgcccc tcgccccgcc ccgccccgcc tggcgcgcgc
     5041 cccccccccc cccccgcccc catcgctgca caaaataatt aaaaaataaa taaatacaaa
     5101 attgggggtg gggagggggg ggagatgggg agagtgaagc agaacgtggg gctcacctcg
     5161 accatggtaa tagcgatgac taatacgtag atgtactgcc aagtaggaaa gtcccataag
     5221 gtcatgtact gggcataatg ccaggcgggc catttaccgt cattgacgtc aatagggggc
     5281 gtacttggca tatgatacac ttgatgtact gccaagtggg cagtttaccg taaatactcc
     5341 acccattgac gtcaatggaa agtccctatt ggcgttacta tgggaacata cgtcattatt
     5401 gacgtcaatg gcctaggcgg cgcgccttaa ttaacctacg acttcctaga gcatggctac
     5461 gtagataagt agcatggcgg gttaatcatt aactacaagg aacccctagt gatggagttg
     5521 gccactccct ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga
     5581 cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag cgcgcagctg
//