pJET1.2-OsMac3

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pJET1.2-OsMac3载体质粒基本信息

质粒名称:	pJET1.2-OsMac3
出品公司:	Wekwikgene
目录编号:	0000448
产品用途:	Cloning vector pJET1.2 (Thermo Fisher) contains the 158-bp 5′-UTR fragment (from −158 to −1 bp before the ATG) of OsMac3 from Japonica rice genomic DNA. The OsMac3 5′-UTR fragment was shown to exhibit sufficient activity as a translational enhancer [Aoki et al., 2014. Plant Biotechnology. 31: 221-228] and can be used to improve genome editing in both monocots [Onodera et al., 2018. PLOS ONE. 13: 1-13] and dicots [Kusano et al., 2018. Scientific Reports 8: 1-9; Kusano et al., 2023. Plant Biotechnology. 40: 201-209]. PROPAGATION: use standard ampicillin concentration (100 mkg/ml) for plasmid propagation.
存储实验室:	Demchenko Lab (BIN RAS)
载体骨架基本信息
载体名称:	pJET1.2
表达系统:	植物表达
启动子:	absent
拷贝数:	高拷贝
载体抗性:	氨苄霉素
生长温度:	37 ℃
宿主菌:	DH5a, XL1Blue, TOP10
插入基因信息
插入基因名称:	OsMac3 5-UTR fragment
物种来源:	Synthetic
密码子优化:	植物表达优化
文献引用方法
参考文献部分:	Do DEEPER ROOTING 1 homologs regulate the lateral root slope angle in cucumber (Cucumis sativus)? Kiryushkin A.S., Ilina E.L., Kiikova T.Y., Pawlowski K., Demchenko K.N. Int. J. Mol. Sci. 2024, Vol. 25, Page 1975 25, 1975 (2024)

pJET1.2-OsMac3质粒图谱载体图谱和pJET1.2-OsMac3载体序列质粒序列多克隆位点信息

pJET1.2-OsMac3载体图谱

pJET1.2-OsMac3质粒载体简介

pJET1.2-OsMac3质粒序列载体序列

LOCUS       pJET1.2-OsMac3               3148 bp DNA     circular SYN 10-DEC-2023
DEFINITION  Linearized positive selection cloning vector with a lethal insert 
            that allows for efficient recovery of blunt-ended PCR products.
ACCESSION   .
VERSION     .
KEYWORDS    pJET1.2-OsMac3
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3148)
  AUTHORS   Thermo Fisher (Fermentas)
  TITLE     Direct Submission
  JOURNAL   Exported Sep 26, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com
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            VNTDBDATE|-15219480|
            LSOWNER|
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            VNTAUTHORNAME|Alex|
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FEATURES             Location/Qualifiers
     source          1..3148
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        complement(9..166)
                     /label=5'-UTR of OsMac3
                     /label=5'-UTR\of\OsMac3
                     /note="/vntifkey=9"
     CDS             complement(175..556)
                     /codon_start=1
                     /gene="eco47IR"
                     /product="mutant version of the Eco47I restriction
                     endonuclease"
                     /label=Eco47I/T7
                     /note="cleaves double-stranded DNA at GGWCC sites in the 
                     absence of protective methylation"
                     /note="/vntifkey=4"
                     /translation="MSKETSFVKNAEELAKQKMDAINPELSSKFKFLIKFLSQFPEACS
                     KPRSKKMQNKVGQEEHIEYLARSFHESRLPRKPTPPTTVPDEVVSIVLNISFNIQPENL
                     ERIKEEHRFSMAAENIVGDLLER"
     misc_feature    175..225
                     /label=MCS
                     /note="multiple cloning site"
                     /note="/vntifkey=21"
     primer_bind     complement(208..231)
                     /label=pJET1.2 reverse sequencing primer
     protein_bind    585..601
                     /label=lac operator
                     /label=lac\operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="/vntifkey=31"
     promoter        complement(609..639)
                     /label=lac UV5 promoter
                     /label=lac\UV5\promoter
                     /note="E. coli lac promoter with an   up   mutation"
                     /note="/vntifkey=30"
     rep_origin      complement(966..1554)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="/vntifkey=33"
     CDS             complement(1725..2585)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="/vntifkey=4"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2586..2690)
                     /gene="bla"
                     /label=AmpR promoter
                     /label=AmpR\promoter
                     /note="/vntifkey=30"
     CDS             complement(2793..3148)
                     /codon_start=1
                     /gene="eco47IR"
                     /product="mutant version of the Eco47I restriction
                     endonuclease"
                     /label=Eco47I/T7
                     /note="cleaves double-stranded DNA at GGWCC sites in the 
                     absence of protective methylation"
                     /note="/vntifkey=4"
                     /translation="ILLKNSSHPEDLAAALPIVSRITPDGYGVQAQVLKQLILFTMMKK
                     TMNGTCSKLKIEIIPKTHRVVRLEIIQQ*KNGLELTHSVMLLIGTIFQMKYHLRI*MKK
                     TSELLLKIIWQK*Y"
     promoter        3082..3100
                     /label=T7 promoter
                     /label=T7\promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="/vntifkey=30"
     primer_bind     3087..3109
                     /label=pJET1.2 forward sequencing primer
     misc_feature    3105..3148
                     /label=MCS
                     /note="multiple cloning site"
                     /note="/vntifkey=21"
ORIGIN
        1 aatctagaat tgcgagacag tgccgtgggt aaatattgtg atctgttctc ttgagctgaa
       61 attcctgcct ttcttcttct acaggctaga gagtcccagg attcagaaaa tgaggagcaa
      121 atcttttgtt gtacttcttt ggtatgaaaa agctctcttt agtctttcta gattatcttt
      181 ctagaagatc tcctacaata ttctcagctg ccatggaaaa tcgatgttct tcttttattc
      241 tctcaagatt ttcaggctgt atattaaaac ttatattaag aactatgcta accacctcat
      301 caggaaccgt tgtaggtggc gtgggttttc ttggcaatcg actctcatga aaactacgag
      361 ctaaatattc aatatgttcc tcttgaccaa ctttattctg catttttttt gaacgaggtt
      421 tagagcaagc ttcaggaaac tgagacagga attttattaa aaatttaaat tttgaagaaa
      481 gttcagggtt aatagcatcc attttttgct ttgcaagttc ctcagcattc ttaacaaaag
      541 acgtctcttt tgacatgttt aaagtttaaa cctcctgtgt gaaattgtta tccgctcaca
      601 attccacaca ttatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
      661 agctaactca cattaattgc gttgcgctca ctgccaattg ctttccagtc gggaaacctg
      721 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
      781 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
      841 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
      901 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
      961 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     1021 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     1081 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     1141 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     1201 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     1261 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     1321 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     1381 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
     1441 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     1501 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     1561 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     1621 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     1681 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     1741 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     1801 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     1861 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     1921 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     1981 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     2041 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     2101 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     2161 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     2221 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     2281 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     2341 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     2401 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     2461 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     2521 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     2581 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     2641 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     2701 cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat
     2761 aggcgtatca cgaggccgcc cctgcagccg aattatatta tttttgccaa ataattttta
     2821 acaaaagctc tgaagtcttc ttcatttaaa ttcttagatg atacttcatc tggaaaattg
     2881 tcccaattag tagcatcacg ctgtgagtaa gttctaaacc atttttttat tgttgtatta
     2941 tctctaatct tactactcga tgagttttcg gtattatctc tatttttaac ttggagcagg
     3001 ttccattcat tgtttttttc atcatagtga ataaaatcaa ctgctttaac acttgtgcct
     3061 gaacaccata tccatccggc gtaatacgac tcactatagg gagagcggcc gccagatctt
     3121 ccggatggct cgagtttttc agcaagat
//

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pJET1.2-OsMac3质粒载体应用举例

上一篇：pCAMBIA1301-proMpEF1a-MpGLK-3xHA
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pQD301	pAAVLINKv1-pCALM1-Cre-3'-CACNA1C-FLAG
pYK464	pER8
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-MYO5C-FLAG	pAAVLINKv2-SCP1-Cre-UDeg3a-LAMA1-C-FLAG
pYe(CEN3+)41	pAAV-AscI-CAG-NES-NeMeHg
pAAVLINKv1-pCALM1-Cre-3'-LAMB1-FLAG	pYK195
pmiRFP713-Actin	pAAV-hSyn-GRAB_OTmut
pAAV-EFS-DIO-GRAB_OTmut	pMAL-p5e
pGL4.80[hRlucP/Neo]	pBD-p53
pAAV-IRES-ZsGreen1	pRSVneo
pHIS1525	pDsRed-Monomer-Golgi
pAAVLINKv1-pCALM1-Cre-WDFY3-C-FLAG	pCMV-mCherry-iPAK4
pAAV-hSyn-GRAB_HA1mut	pUMVC
pET-37b(+)	pCytERM-Electra2
pDEST15	pAAV-CAG-GINKO2
pCMV-PE5max-EMX1-hCtRNA-PegRNA-hCtRNA-Ni	pAAV-CAG-NES-FGCaMP7
pUBC-1M3U-EGFP	pAAVLINK-EF1a-NOTCH1-A
pUG66	pAAVLINK-EF1a-HA-5'-KIF14
pAAVLINKv1-pCALM1-Cre-FLNA-C-FLAG	pCDH-CMV-mCherry-RRV capsid
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-UNC13A-FLA	pET-11b(+)
pET301/CT-DEST	pcDNA3-HA-TAB1
pCAMBIA1301-35S-AtMYBS1-GFP	pAcGFP1-N1
pQE-1	pTre3G-mEGFP-iPAK4-CMV-rtTA3
pYX212	pMHE3Tc
pYK371	pET28a-EGFR_nB
pET28a-sumo	pCR9-10
pKSE-vCas9-ipB7RKD	pU6-sgC2-VEGFA T1
pCMV-Tag 4B	YEp351
pET-15b	pCytERM-EBFP2
pND201	PCDH-CMV-mCherry-TRIM15
pGADT7	pAAV-CaMKII-ElectraOFF-Kv2.1
pMBP-QM-Man	pHh5G(H2a)
pGDP1 NDM-1-Amp	pCAMBIA1301-35S-AtBBX14-GFP
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-PCM1-FLAG	pCSN44
pAAV-CAG-BDFP1.6	pAAVLINKv2-SCP1-Cre-UDeg3a-3'-CRISPRoff-
pQL281	pEYFP-Golgi
pKO11	pNC36
pElectra2-Vimentin	pAAV-TNT4-Zfr-P2A-HA-luciferase
psiCHECK-2-mBJ-SYT1_wt-mScarlet3	psiCHECK-2-h1-cluster13
PCDH-TetO-mEGFP-TRIM27-TurboID	pET-12c(+)
pNuc1	pEBFP-N1
pAD22	pLZ141
pAc5.1/V5-His B	pCDF1-MCS2-EF1-copGFP
pCAMBIA1301-proMpEF1a-MpLFY-5xMyc	pAAV-hSyn-GRAB-rDA2mut
pIAβ8	p304
pHV11	pGEX-4T-3
pBR327par	PCDH-CMV-Hotag3-FKBP-mCherry-TRIM6 L148P
pGreenII0179	pYK521
pCytERM-miRFP670-2-N1	PCDH-CMV-mCherry
pSilencer1.0	pAAVLINKv1-pCALM1-Cre-3'-SMARCA4-FLAG
pH2B-iRFP670	pAAV-CAG-mRhubarb719

PSD95-TS:YFP	circRNA-synIRES-R25-NanoLuc
pEGFP-N1-FLAG	Flag-HA-USP42
pBLO 62.5	MLCK_pLVX-EF1-Cry2-mCherry
pGL4-ERSE1-luc2P-Hygro	pCMVβ-p300-myc
pGWB454	pHAGE2-TetOminiCMV-SV40LT
MS2-P65-HSF1_GFP	pGD-P1/HC-Pro
pDA077	pRRLsin-SV40 T antigen-IRES-mCherry
pCDNA3.1 (-) + SLNCR1 + 12x MS2 bs	pSF3-Flag-CBir-FRB_Myc-NBir-FKBP
pmTurquoise2-Golgi	pNK1
DT480	pCasPA
Lenti-Hb9-GFP	pSin Capsid
pAAV-hSyn-SOUL-P2A-tdTomato	Cyto-roGFP
pDule2-pCNF	human beta-catenin pcDNA3
pBV-Luc	pUCmini-iCAP-PHP.eB
pF4Ag NanoLuc (ATG-42)	pOpen-9N7polA
pAWP78-PVCpnf_pvc13-HA	cytoplasmic EKAR (EGFP-mRFP)
TAL3392	pHAGE_puro
pCT302	Fuw-dCas9-Dnmt3a_IM
CMV-CASANOVA	pSaCas9_GFP
pT7-VP7SA11	Mfn2-YFP
pCMV-Tag2B EDD	CoV2-Spike-D614G
pNG3	FUtdTW
LAMP1-mGFP	D. vulgaris Cascade/I-C (Cas5c-Cas8c-Cas
BL21(DE3)-R3-pRARE2	pCAG-FlpeERT2
pAra-tns	pLenti X1 Puro DEST (694-6)
pBUE411-GGB	pQCascade(Ptr)-entry(BsaI)
LentiGuide-zeo	pLenti6-V5-p53_R273H
LentiCRISPRv2-ATG5	AAV-U6-dgRNA-CAG-MPH
pFE-sfGFP	pTnsABC
pCMV2-SEP-GluA1 (M1)	G1397 DddAtox-N–SaKKH-Cas9(D10A)
pcDNA3.1(+) Drp1	pTNS2
v3em-Cterm-PE2max-RNaseH-dualU6	pT2K-CAGGS-T2TP
pRK5-HA-Ubiquitin-K11	pJK-caCas9-NatMX-Neut5L-Ade2 drive
pMXspuro-GFP	p2CT-His-MBP-Lbu_C2c2_R472A_H477A_R1048A
pTC223	pLX304 CMV FKBP-V5-sTurboID (N)
TLCV2-APOBEC1-YTH	PTEF1-yEGFPCLN2PEST-pRS406
pHLmMBP-10	pMP71-HLA-A0201-His
pnEA-NpHG_N	pLOVE empty vector
pSBY1_FnCpf1cg	pCMV-dCas13-M3nls
pVE2_3_p40S_QfDBD_VP16AD_cp_pcbC_DsRed_1	pTAT-HA
pEVOL-AckRS	pDO23A
pF141 pAcGFP1 SOD1WT	pASKt15C+SrtA
pLV-egfp-U3-Osp.gRNA	pVAX_CBI_AcrIIA5_D4276
pOSIP-TT (TetR, P21)	EGFR WT
pDule-pCNF	pRK5-p18-HA
pBAD-Flp	pAWP78-PVCpnf_pvc13-truncated
pDEST17-Tpt1	mTagBFP-Lifeact-7
pHAGE TRE dCas9-KRAB	pSLIK CA Rac1
pYLsgRNA-AtU6-1	pX600-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA
PB533_15F11-sfGFP	pEBB-BT-Ubiquitin

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