| 质粒名称: | pGDP1 NDM-1-Amp |
|---|---|
| 目录编号: | V2609 |
| 存储实验室: | Biofeng Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pBR322 |
| 载体抗性: | 氨苄霉素 |
| 载体类型: | 细菌表达 |
| 高拷贝/低拷贝: | 低拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | NDM-1 |
| 物种来源: | Klebsiella pneumoniae |
| 启动子 | Pbla |
| 标签/融合蛋白: | 6xHis（主干上的N终端） |
| 克隆方法: | 限制性内切酶 |
| 5' 测序引物 | CATGAGACAATAACCCTG |
| 3' 测序引物 | CCAACTCAGCTTCCTTTC |
|
文献引用方法 |
|
| 材料和方法部分: | pGDP1 NDM-1 was a gift from Biofeng. |
pGDP1 NDM-1-Amp
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pGDP1 NDM-1-Amp载体质粒基本信息
pGDP1 NDM-1-Amp质粒图谱载体图谱和pGDP1 NDM-1-Amp载体序列质粒序列多克隆位点信息
pGDP1 NDM-1-Amp质粒载体简介
pGDP1 NDM-1-Amp质粒序列载体序列
Snapgene载体序列下载: pGDP1_NDM-1-Amp序列 .gb格式载体序列下载:pGDP1_NDM-1-Amp载体序列
LOCUS pGDP1_NDM-1-Amp 4072 bp DNA circular SYN 21-JUN-2026
DEFINITION .
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4072)
AUTHORS Biofeng.com
TITLE Direct Submission
JOURNAL Exported Jun 21, 2026 from SnapGene 8.1.0
https://www.biofeng.com
COMMENT Sequence Label: pGDP1 NDM-1-Amp
FEATURES Location/Qualifiers
source 1..4072
/mol_type="other DNA"
/organism="synthetic DNA construct"
RBS 232..254
/note="efficient ribosome binding site from bacteriophage
T7 gene 10 (Olins and Rangwala, 1989)"
/note="color: #a6acb3"
CDS 273..290
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/note="color: #cc99b2"
/translation="HHHHHH"
CDS 300..317
/codon_start=1
/product="thrombin recognition and cleavage site"
/label=thrombin site
/note="color: #cc99b2
Cleavage site after base 311"
/translation="LVPRGS"
CDS 1180..1197
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/note="color: #cc99b2"
/translation="HHHHHH"
terminator 1259..1286
/label=rrnB T2 terminator
/note="transcription terminator T2 from the E. coli rrnB
gene"
/note="color: #ffffff"
CDS complement(join(1377..2168,2169..2237))
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/note="This feature has 2 segments:
1: 1377 .. 2168 / #ccffcc
2: 2169 .. 2237 / #ccffcc / signal sequence
Cleavage site after base 2168"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(2238..2342)
/gene="bla"
/label=AmpR promoter
/note="color: #ffffff; direction: LEFT"
rep_origin 2464..3052
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
/note="color: #ffff00"
misc_feature 3238..3377
/label=bom
/note="basis of mobility region from pBR322"
/note="color: #a6acb3"
CDS complement(3479..3670)
/codon_start=1
/gene="rop"
/product="Rop protein, which maintains plasmids at low copy
number"
/label=rop
/note="color: #993366"
/translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
DELYRSCLARFGDDGENL"
ORIGIN
1 agtaaggcaa ccccgccagc ctagccggga gatctggcct attggttaaa aaatgagctg
61 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca
121 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
181 tgtatccgct catgagacaa taaccctgat aaatgcttct ctagaaataa ttttgtttaa
241 ctttaagaag gagatatacc atgggcagca gccatcatca tcatcatcac agcagcggcc
301 tggtgccgcg cggcagccat atggaactgc cgaacattat gcacccggtt gcgaaactga
361 gcacggccct ggcagcagcc ctgatgctga gcggttgtat gccgggtgaa attcgtccga
421 ccatcggcca gcaaatggaa acgggcgatc agcgttttgg tgacctggtg ttccgtcagc
481 tggcaccgaa cgtttggcaa catacctcat atctggatat gccgggcttt ggtgcagttg
541 cttcgaatgg tctgatcgtt cgtgatggtg gtcgtgtcct ggtggttgac accgcatgga
601 cggatgacca gaccgcccaa attctgaact ggatcaaaca ggaaattaat ctgccggtcg
661 cactggcagt cgtgacccat gcacaccaag ataaaatggg tggtatggac gcactgcatg
721 cagcaggcat tgctacgtat gcaaacgctc tgtccaatca gctggcgccg caagaaggta
781 tggtggcagc tcagcacagc ctgacctttg cagcaaacgg ttgggtcgaa ccggcaacgg
841 ccccgaattt tggtccgctg aaagtgttct acccgggccc gggtcacacc agtgataaca
901 tcacggttgg cattgatggt accgacatcg ccttcggcgg ttgcctgatt aaagatagta
961 aagcgaaatc cctgggcaat ctgggtgatg ccgacacgga acattacgca gctagcgcac
1021 gtgcttttgg tgcagcattc ccgaaagcat ctatgatcgt gatgagccac tctgcaccgg
1081 attcacgcgc tgcgattacc cacaccgccc gtatggctga caaactgcgt tgaggatccg
1141 aattcgagct ccgtcgacaa gcttgcggcc gcactcgagc accaccacca ccaccactga
1201 gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa
1261 aaggccatcc gtcaggatgg ccttctggtg gcacttttcg gggaaatgtg cgcggaaccc
1321 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaat taattcttac
1381 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
1441 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
1501 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
1561 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
1621 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
1681 gttgccattg ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
1741 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
1801 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
1861 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
1921 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
1981 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
2041 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
2101 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
2161 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
2221 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
2281 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
2341 cgaacacccc ttgtattact gtttatgtaa gcagacagtt ttattgttca tgaccaaaat
2401 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
2461 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
2521 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
2581 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca
2641 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
2701 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
2761 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
2821 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
2881 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
2941 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
3001 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
3061 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
3121 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
3181 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct
3241 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcaatg gtgcactctc
3301 agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg
3361 actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
3421 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
3481 agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt
3541 ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct
3601 ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct
3661 gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga
3721 tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg
3781 aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc
3841 agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc
3901 atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac
3961 tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc
4021 agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa cc
//
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