| 质粒名称: | pAAVLINKv1-pCALM1-Cre-FLNA-C-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001753 |
| 产品用途: | 3' AAVLINK plasmid for FLNA expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | pCALM1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | FLNA |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINKv1-pCALM1-Cre-FLNA-C-FLAG
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINKv1-pCALM1-Cre-FLNA-C-FLAG载体质粒基本信息
pAAVLINKv1-pCALM1-Cre-FLNA-C-FLAG质粒图谱载体图谱和pAAVLINKv1-pCALM1-Cre-FLNA-C-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv1-pCALM1-Cre-FLNA-C-FLAG质粒载体简介
pAAVLINKv1-pCALM1-Cre-FLNA-C-FLAG质粒序列载体序列
LOCUS pAAVLINKv1-pCALM1-Cre-FLNA-C-FLAG 6849 bp DNA circular SYN 21-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6849)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 5, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6849
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 145..265
/label=pCALM1
CDS 277..1308
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
intron 1309..1349
/label=Splicing Donor
protein_bind 1356..1389
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1390..1481
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1482..3562
/codon_start=1
/label=FLNA
/translation="VTTPCVCPT*RSALLPTSPSTSQRRISAC*RPLWSRPRAGRSPVC
*SGCVMATWGFHSCPRRRGSTWCM*RKMASTWPAAPSRW*SASRKLGMPVVFGSLVRAF
TKATPLSLQSLSLIPAMQAMVGSACPLRAPARWTSTQRTWRTGRAGSPTAPQSQATTSS
TSSLPTSTCLAAPSL*R*QARAG*KRASPAGVGLLQWPTLVVIVTSA*KSLKLASRI*Q
PR*PAHRARPMRPRSWKGRTTPTASALFPLRWAHTQSA*STRASTCLGAPSSSPWGP*G
KGEPTRSELGALAWRELKLECQPNSVSGPGKLVLEAWPLLSRAPARLRSLLRTARTAPV
VWLMWSRSQVTTKSQSSSTRNTFPTAPSWCLWLLRLATPAASLFLAFRSQG*RSTSQPL
LQSA*TGPRGRSMPRCTAPQEPWRSAMSQKLTKISMLCASSLGRMAFT*LTSSSTAPTS
LEAPSRSELGSLGMEGTQAWCLLTEQVWKAVSQGTQLSSS*TRAMRELVPCR*PLTAPP
R*RWIARSALRATASPIPPWHLAATSSPSSTAAPTTLGAAPSRPKSQAPVSSATTASTR
HHQCL*TL*PRPPVPPSMGPRVLGLLTPARWWPRAWG*ARPT*ARRAASQ*TAAKQATT
CCWWGFMAQGPPARRSW*STWAAGSTACPTCSRTRGSTHWWSNGGTSTSQAAPTALWC"
CDS 3569..3634
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3638..4069
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3782..3787
/label=SDS
repeat_region 4112..4252
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4112..4241
/label=AAV2 ITR
rep_origin 4327..4782
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5064..5168
/gene="bla"
/label=AmpR promoter
CDS 5169..6029
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6200..6788
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
1441 tattggtctt actgacatcc actttgcctt tctctccaca ggtgacgact ccatgcgtat
1501 gtcccaccta aaggtcggct ctgctgccga catccccatc aacatctcag agacggatct
1561 cagcctgctg acggccactg tggtcccgcc ctcgggccgg gaggagccct gtttgctgaa
1621 gcggctgcgt aatggccacg tggggatttc attcgtgccc aaggagacgg gggagcacct
1681 ggtgcatgtg aagaaaaatg gccagcacgt ggccagcagc cccatcccgg tggtgatcag
1741 ccagtcggaa attggggatg ccagtcgtgt tcgggtctct ggtcagggcc ttcacgaagg
1801 ccacaccttt gagcctgcag agtttatcat tgatacccgc gatgcaggct atggtgggct
1861 cagcctgtcc attgagggcc ccagcaaggt ggacatcaac acagaggacc tggaggacgg
1921 gacgtgcagg gtcacctact gccccacaga gccaggcaac tacatcatca acatcaagtt
1981 tgccgaccag cacgtgcctg gcagcccctt ctctgtgaag gtgacaggcg agggccgggt
2041 gaaagagagc atcacccgca ggcgtcgggc tccttcagtg gccaacgttg gtagtcattg
2101 tgacctcagc ctgaaaatcc ctgaaattag catccaggat atgacagccc aggtgaccag
2161 cccatcgggc aagacccatg aggccgagat cgtggaaggg gagaaccaca cctactgcat
2221 ccgctttgtt cccgctgaga tgggcacaca cacagtcagc gtgaagtaca agggccagca
2281 cgtgcctggg agccccttcc agttcaccgt ggggccccta ggggaagggg gagcccacaa
2341 ggtccgagct gggggccctg gcctggagag agctgaagct ggagtgccag ccgaattcag
2401 tatctggacc cgggaagctg gtgctggagg cctggccatt gctgtcgagg gccccagcaa
2461 ggctgagatc tcttttgagg accgcaagga cggctcctgt ggtgtggctt atgtggtcca
2521 ggagccaggt gactacgaag tctcagtcaa gttcaacgag gaacacattc ccgacagccc
2581 cttcgtggtg cctgtggctt ctccgtctgg cgacgcccgc cgcctcactg tttctagcct
2641 tcaggagtca gggctaaagg tcaaccagcc agcctctttt gcagtcagcc tgaacggggc
2701 caagggggcg atcgatgcca aggtgcacag cccctcagga gccctggagg agtgctatgt
2761 cacagaaatt gaccaagata agtatgctgt gcgcttcatc cctcgggaga atggcgttta
2821 cctgattgac gtcaagttca acggcaccca catccctgga agccccttca agatccgagt
2881 tggggagcct gggcatggag gggacccagg cttggtgtct gcttacggag caggtctgga
2941 aggcggtgtc acagggaacc cagctgagtt cgtcgtgaac acgagcaatg cgggagctgg
3001 tgccctgtcg gtgaccattg acggcccctc caaggtgaag atggattgcc aggagtgccc
3061 tgagggctac cgcgtcacct atacccccat ggcacctggc agctacctca tctccatcaa
3121 gtacggcggc ccctaccaca ttgggggcag ccccttcaag gccaaagtca caggcccccg
3181 tctcgtcagc aaccacagcc tccacgagac atcatcagtg tttgtagact ctctgaccaa
3241 ggccacctgt gccccccagc atggggcccc gggtcctggg cctgctgacg ccagcaaggt
3301 ggtggccaag ggcctggggc tgagcaaggc ctacgtaggc cagaagagca gcttcacagt
3361 agactgcagc aaagcaggca acaacatgct gctggtgggg gttcatggcc caaggacccc
3421 ctgcgaggag atcctggtga agcacgtggg cagccggctc tacagcgtgt cctacctgct
3481 caaggacaag ggggagtaca cactggtggt caaatggggg gacgagcaca tcccaggcag
3541 cccctaccgc gttgtggtgc cctgtacaga ctacaaagac catgacggtg attataaaga
3601 tcatgacatc gactacaagg atgacgatga caagtaactc gagataatca acctctggat
3661 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
3721 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
3781 tcctccttgt ataaatcctg gttagttctt gccacggcgg aactcatcgc cgcctgcctt
3841 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gtttatttgt
3901 gaaatttgtg atgctattgc tttatttgta accatctagc tttatttgtg aaatttgtga
3961 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg
4021 cattcatttt atgtttcagg ttcaggggga gatgtgggag gttttttaaa gatctggaga
4081 ccggtgtggc acgtgcggac cgagcggccg caggaacccc tagtgatgga gttggccact
4141 ccctctctgc gcgctcgctc gctcactgag gccgggcgac caaaggtcgc ccgacgcccg
4201 ggctttgccc gggcggcctc agtgagcgag cgagcgcgca gctgcctgca ggggcgcctg
4261 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatacg tcaaagcaac
4321 catagtacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
4381 tgaccgctac acttgccagc gccttagcgc ccgctccttt cgctttcttc ccttcctttc
4441 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
4501 gatttagtgc tttacggcac ctcgacccca aaaaacttga tttgggtgat ggttcacgta
4561 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
4621 atagtggact cttgttccaa actggaacaa cactcaactc tatctcgggc tattcttttg
4681 atttataagg gattttgccg atttcggtct attggttaaa aaatgagctg atttaacaaa
4741 aatttaacgc gaattttaac aaaatattaa cgtttacaat tttatggtgc actctcagta
4801 caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg
4861 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
4921 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc
4981 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
5041 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
5101 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
5161 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
5221 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
5281 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
5341 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
5401 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
5461 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
5521 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
5581 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
5641 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
5701 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
5761 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
5821 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
5881 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
5941 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
6001 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
6061 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
6121 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
6181 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
6241 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
6301 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
6361 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
6421 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
6481 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
6541 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
6601 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
6661 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
6721 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
6781 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
6841 ctcacatgt
//
