| 质粒名称: | pAAV-TNT4-Zfr-P2A-HA-luciferase |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001192 |
| 产品用途: | in vivo |
| 存储实验室: | Yuxuan Guo Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV |
| 表达系统: | 腺病毒表达 |
| 启动子: | hTNNT2 |
| 拷贝数: | 未拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | NEB Stable |
|
插入基因信息 |
|
| 插入基因名称: | luciferase |
| 物种来源: | MUS |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Neural Organoids Protect Engineered Heart Tissues From Glucolipotoxicity by Transferring Versican in a Co-Culture System.
Bai B., Li J., Wang Z., Yang Y., He J., Chen G., Zhang Y., Qi Y., Wan Z., Cai L., Wang R., Wang K., Zhao D., Zhang J., Huang W., Xu X.R., Sun M., Han X., Liu Y., Zhang D., Zhu W., Liu J., Guo Y.
Cell Prolif. 0, e70070 (2025)
|
买家导航
pAAV-TNT4-Zfr-P2A-HA-luciferase载体质粒基本信息
pAAV-TNT4-Zfr-P2A-HA-luciferase质粒图谱载体图谱和pAAV-TNT4-Zfr-P2A-HA-luciferase载体序列质粒序列多克隆位点信息
pAAV-TNT4-Zfr-P2A-HA-luciferase质粒载体简介
pAAV-TNT4-Zfr-P2A-HA-luciferase质粒序列载体序列
LOCUS pAAV-TNT4-Zfr-P2A-HA-luciferase 6947 bp ds-DNA circular 21-JUN-2024
DEFINITION .
COMMENT ^^
FEATURES Location/Qualifiers
misc_feature 1..130
/label="5' ITR"
/ApEinfo_revcolor="#d59687"
/ApEinfo_fwdcolor="#d59687"
misc_feature 29..34
/label="XmaI"
/ApEinfo_revcolor="#85dae9"
/ApEinfo_fwdcolor="#85dae9"
misc_feature 40..45
/label="XmaI"
/ApEinfo_revcolor="#84b0dc"
/ApEinfo_fwdcolor="#84b0dc"
misc_feature 176..197
/label="overlap"
/ApEinfo_revcolor="#ffef86"
/ApEinfo_fwdcolor="#ffef86"
primer 176..218
/label="cmv2TNT4-clone-F"
/note="sequence: TGGGGATCCTCTAGAACTATAGCTCAGTCCATTAGGAGCCAGT"
/ApEinfo_revcolor="#f8d3a9"
/ApEinfo_fwdcolor="#f8d3a9"
misc_feature 178..197
/label="nhe1cut"
/ApEinfo_revcolor="#f58a5e"
/ApEinfo_fwdcolor="#f58a5e"
misc_feature 198..218
/label="overlap"
/ApEinfo_revcolor="#f58a5e"
/ApEinfo_fwdcolor="#f58a5e"
misc_feature 198..741
/label="hTNT promoter"
/ApEinfo_revcolor="#faac61"
/ApEinfo_fwdcolor="#faac61"
misc_feature 737..756
/label="cut of not1"
/ApEinfo_revcolor="#75c6a9"
/ApEinfo_fwdcolor="#75c6a9"
primer 737..777
/label="tnt4-zn-clone-F"
/note="sequence: GGCAGagctCCTCGAGAAGcACTGACCAACAGCGTGAAGAC"
/ApEinfo_revcolor="#ff9ccd"
/ApEinfo_fwdcolor="#ff9ccd"
misc_feature 757..777
/label="overlap"
/ApEinfo_revcolor="#75c6a9"
/ApEinfo_fwdcolor="#75c6a9"
exon 757..795
/label="Zfr-201 Exon 19"
/ApEinfo_revcolor="#c6c9d1"
/ApEinfo_fwdcolor="#c6c9d1"
misc_feature 757..795
/label="upstream"
/ApEinfo_revcolor="#f8d3a9"
/ApEinfo_fwdcolor="#f8d3a9"
gene 757..1989
/label="Zfr minigene(1233BP)"
/ApEinfo_revcolor="#b1ff67"
/ApEinfo_fwdcolor="#b1ff67"
primer 769..792
/label="Zfr-rtpcr-F"
/note="sequence: CGTGAAGACATTACATCCAGTGCA"
/ApEinfo_revcolor="#c6c9d1"
/ApEinfo_fwdcolor="#c6c9d1"
misc_feature 841..930
/label="I1D1"
/ApEinfo_revcolor="#d6b295"
/ApEinfo_fwdcolor="#d6b295"
misc_feature 841..1200
/label="to delete"
/ApEinfo_revcolor="#f8d3a9"
/ApEinfo_fwdcolor="#f8d3a9"
misc_feature 931..1020
/label="I1D2"
/ApEinfo_revcolor="#75c6a9"
/ApEinfo_fwdcolor="#75c6a9"
misc_feature 1021..1110
/label="I1D3"
/ApEinfo_revcolor="#85dae9"
/ApEinfo_fwdcolor="#85dae9"
misc_feature 1111..1200
/label="I1D4"
/ApEinfo_revcolor="#ff9ccd"
/ApEinfo_fwdcolor="#ff9ccd"
misc_feature 1261..1264
/label="bp"
/ApEinfo_revcolor="#75c6a9"
/ApEinfo_fwdcolor="#75c6a9"
misc_feature 1296..1379
/label="PSEX"
/ApEinfo_revcolor="#faac61"
/ApEinfo_fwdcolor="#faac61"
CDS 1299..1379
/ApEinfo_revcolor="#84b0dc"
/ApEinfo_fwdcolor="#84b0dc"
/translation="KGLHIKNIV*TL*GQRSVLISPD*KL*"
primer 1345..1369
/label="Zfr-qpcr-F"
/note="sequence: GAAGTGTGCTCATTTCTCCCGATTA"
/ApEinfo_revcolor="#c6c9d1"
/ApEinfo_fwdcolor="#c6c9d1"
CDS 1376..1379
/ApEinfo_revcolor="#84b0dc"
/ApEinfo_fwdcolor="#84b0dc"
/translation="M"
misc_feature 1380..1948
/label="keep"
/ApEinfo_revcolor="#84b0dc"
/ApEinfo_fwdcolor="#84b0dc"
primer complement(1390..1431)
/label="tnt4-zn-clone-R"
/note="sequence: aaaccctgtttcaaaTCTCCCACCCCCAAAAGGCAAAATACC"
/ApEinfo_revcolor="#ffef86"
/ApEinfo_fwdcolor="#ffef86"
misc_feature 1412..1431
/label="overlap"
/ApEinfo_revcolor="#b1ff67"
/ApEinfo_fwdcolor="#b1ff67"
primer 1412..1453
/label="zn-luci-clone-F"
/note="sequence: GGAGAtttgaaacagggtttctccttacagctctggctttcc"
/ApEinfo_revcolor="#faac61"
/ApEinfo_fwdcolor="#faac61"
misc_feature 1433..1522
/label="I2D1"
/ApEinfo_revcolor="#c6c9d1"
/ApEinfo_fwdcolor="#c6c9d1"
misc_feature 1433..1882
/label="to delete"
/ApEinfo_revcolor="#d6b295"
/ApEinfo_fwdcolor="#d6b295"
misc_feature 1523..1612
/label="I2D2"
/ApEinfo_revcolor="#f8d3a9"
/ApEinfo_fwdcolor="#f8d3a9"
misc_feature 1613..1702
/label="I2D3"
/ApEinfo_revcolor="#c6c9d1"
/ApEinfo_fwdcolor="#c6c9d1"
misc_feature 1703..1792
/label="I2D4"
/ApEinfo_revcolor="#f58a5e"
/ApEinfo_fwdcolor="#f58a5e"
misc_feature 1793..1882
/label="I2D5"
/ApEinfo_revcolor="#ff9ccd"
/ApEinfo_fwdcolor="#ff9ccd"
misc_feature 1933..1937
/label="bp"
/ApEinfo_revcolor="#f8d3a9"
/ApEinfo_fwdcolor="#f8d3a9"
misc_feature 1949..1989
/label="downstream"
/ApEinfo_revcolor="#b4abac"
/ApEinfo_fwdcolor="#b4abac"
exon 1949..1989
/label="Zfr-201 Exon 20"
/ApEinfo_revcolor="#9eafd2"
/ApEinfo_fwdcolor="#9eafd2"
CDS 1951..1989
/ApEinfo_revcolor="#84b0dc"
/ApEinfo_fwdcolor="#84b0dc"
/translation="CIETPCIPSDTQS"
misc_feature 1966..1989
/label="overlap"
/ApEinfo_revcolor="#d59687"
/ApEinfo_fwdcolor="#d59687"
misc_feature 1990..2046
/label="P2A"
/ApEinfo_revcolor="#75c6a9"
/ApEinfo_fwdcolor="#75c6a9"
CDS 1990..2046
/ApEinfo_revcolor="#84b0dc"
/ApEinfo_fwdcolor="#84b0dc"
/translation="ATNFSLLKQAGDVEENPGP"
primer complement(2003..2025)
/label="P2A-R1"
/note="sequence: gtctccagcctgTttTagTaggc"
/ApEinfo_revcolor="#b4abac"
/ApEinfo_fwdcolor="#b4abac"
primer complement(2005..2029)
/label="P2A-R2"
/note="sequence: cAacgtctccagcctgTttTagTag"
/ApEinfo_revcolor="#f58a5e"
/ApEinfo_fwdcolor="#f58a5e"
misc_feature 2007..2028
/label="non-AUG start codons removed"
/ApEinfo_revcolor="#75c6a9"
/ApEinfo_fwdcolor="#75c6a9"
primer complement(2011..2031)
/label="P2A-R5"
/note="sequence: ctcAacgtctccagcctgTtt"
/ApEinfo_revcolor="#f58a5e"
/ApEinfo_fwdcolor="#f58a5e"
primer complement(2017..2037)
/label="P2A-R3"
/note="sequence: gttctcctcAacgtctccagc"
/ApEinfo_revcolor="#ff9ccd"
/ApEinfo_fwdcolor="#ff9ccd"
primer complement(2025..2044)
/label="P2A-R4"
/note="sequence: gtccagggttctcctcAacg"
/ApEinfo_revcolor="#75c6a9"
/ApEinfo_fwdcolor="#75c6a9"
primer complement(2036..2077)
/label="zn-luci-clone-R"
/note="sequence: cttctGCGTAATCTGGAACGTCgTATGGATAaggtccagggt"
/ApEinfo_revcolor="#85dae9"
/ApEinfo_fwdcolor="#85dae9"
CDS 2047..2070
/ApEinfo_revcolor="#84b0dc"
/ApEinfo_fwdcolor="#84b0dc"
/translation="YPYDVPDY"
misc_feature 2047..2073
/label="HA tag"
/ApEinfo_revcolor="#d6b295"
/ApEinfo_fwdcolor="#d6b295"
CDS 2047..2098
/ApEinfo_revcolor="#84b0dc"
/ApEinfo_fwdcolor="#84b0dc"
/translation="YPYDVPDYAEDAKNIKK"
misc_feature 2059..2077
/label="OVERLAP"
/ApEinfo_revcolor="#85dae9"
/ApEinfo_fwdcolor="#85dae9"
primer 2059..2101
/label="ha-luci-clone-F"
/note="sequence: GTTCCAGATTACGCagaagacgccaaaaacataaagaaaggcc"
/ApEinfo_revcolor="#b7e6d7"
/ApEinfo_fwdcolor="#b7e6d7"
CDS 2074..2086
/label="Translation 2074-2086"
/translation="EDAK"
CDS 2074..3722
/label="luciferase"
/ApEinfo_revcolor="#ffef86"
/ApEinfo_fwdcolor="#ffef86"
/note="EC_number: product: firefly luciferase codon_start: 1 translation: MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDAHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAPANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAV* transl_table: 1 gene: luc+ note: enhanced luc+ version of the luciferase gene protein_id: "
CDS 2074..3722
/label="Translation 2074-3722"
/translation="EDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDAHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAPANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKMPC"
primer complement(2169..2188)
/label="DM-St-luci-qPCR-R1"
/note="sequence: ttgttccaggaaccagggcg"
/ApEinfo_revcolor="#9eafd2"
/ApEinfo_fwdcolor="#9eafd2"
primer complement(2541..2584)
/label="HA-luci-clone-R"
/note="sequence: gatgagatgtgacgaacgtgtacatcgactgaaatccctggtaa"
/ApEinfo_revcolor="#d6b295"
/ApEinfo_fwdcolor="#d6b295"
misc_feature 2566..2584
/label="cut of bsrg1"
/ApEinfo_revcolor="#d59687"
/ApEinfo_fwdcolor="#d59687"
primer 3082..3104
/label="luciferase-F"
/note="sequence: caaggatatgggctcactgagac"
/ApEinfo_revcolor="#ffef86"
/ApEinfo_fwdcolor="#ffef86"
misc_feature 3082..3303
/label="qpcr-sequence"
/ApEinfo_revcolor="#75c6a9"
/ApEinfo_fwdcolor="#75c6a9"
primer complement(3190..3207)
/label="Lucifer-seq3"
/note="sequence: cccggtatccagatccac"
/ApEinfo_revcolor="#d59687"
/ApEinfo_fwdcolor="#d59687"
primer complement(3286..3303)
/label="luciferase-R"
/note="sequence: caaggcgttggtcgcttc"
/ApEinfo_revcolor="#c7b0e3"
/ApEinfo_fwdcolor="#c7b0e3"
misc_feature 3704..3722
/label="overlap"
/ApEinfo_revcolor="#c6c9d1"
/ApEinfo_fwdcolor="#c6c9d1"
CDS 3721..3739
/ApEinfo_revcolor="#84b0dc"
/ApEinfo_fwdcolor="#84b0dc"
/translation="KYL*SR"
misc_feature 3723..3727
/label="KpnI"
/ApEinfo_revcolor="#f58a5e"
/ApEinfo_fwdcolor="#f58a5e"
misc_feature 3723..3741
/label="cutting site"
/ApEinfo_revcolor="#9eafd2"
/ApEinfo_fwdcolor="#9eafd2"
misc_feature 3739..3744
/label="XmaI"
/ApEinfo_revcolor="#b1ff67"
/ApEinfo_fwdcolor="#b1ff67"
terminator 3782..3908
/label="PolyA"
/ApEinfo_revcolor="#9eafd2"
/ApEinfo_fwdcolor="#9eafd2"
misc_feature 3997..4126
/label="3' ITR"
/ApEinfo_revcolor="#b7e6d7"
/ApEinfo_fwdcolor="#b7e6d7"
misc_feature 4082..4087
/label="XmaI"
/ApEinfo_revcolor="#c7b0e3"
/ApEinfo_fwdcolor="#c7b0e3"
misc_feature 4093..4098
/label="XmaI"
/ApEinfo_revcolor="#84b0dc"
/ApEinfo_fwdcolor="#84b0dc"
rep_origin 4303..4758
/label="f1 origin"
/ApEinfo_revcolor="#f58a5e"
/ApEinfo_fwdcolor="#f58a5e"
misc_feature 4784..4888
/label="Amp Promoter"
/ApEinfo_revcolor="#f58a5e"
/ApEinfo_fwdcolor="#f58a5e"
misc_feature 4889..5746
/label="Amp resistent"
/ApEinfo_revcolor="#ff9ccd"
/ApEinfo_fwdcolor="#ff9ccd"
rep_origin 5920..6508
/label="origin"
/ApEinfo_revcolor="#85dae9"
/ApEinfo_fwdcolor="#85dae9"
ORIGIN
1 CTGCGCGCTC GCTCGCTCAC TGAGGCCGCC CGGGCAAAGC CCGGGCGTCG GGCGACCTTT
61 GGTCGCCCGG CCTCAGTGAG CGAGCGAGCG CGCAGAGAGG GAGTGGCCAA CTCCATCACT
121 AGGGGTTCCT TGTAGTTAAT GATTAACCCG CCATGCTACT TATCTACCAG GGTAATGGGG
181 ATCCTCTAGA ACTATAGCTC AGTCCATTAG GAGCCAGTAG CCTGGAAGAT GTCTTTACCC
241 CCAGCATCAG TTCAAGTGGA GCAGCACATA ACTCTTGCCC TCTGCCTTCC AAGATTCTGG
301 TGCTGAGACT TATGGAGTGT CTTGGAGGTT GCCTTCTGCC CCCCAACCCT GCTCCCAGCT
361 GGCCCTCCCA GGCCTGGGTT GCTGGCCTCT GCTTTATCAG GATTCTCAAG AGGGACAGCT
421 GGTTTATGTT GCATGACTGT TCCCTGCATA TCTGCTCTGG TTTTAAATAG CTTATCTGAG
481 CAGCTGGAGG ACCACATGGG CTTATATGGC GTGGGGTACA TGTTCCTGTA GCCTTGTCCC
541 TGGCACCTGC CAAAATAGCA GCCAACAccc cccaccccca ccgccatccc cctgccccac
601 ccgtccccTG TCGCACATTC CTCCCTCCGC AGGGCTGGCT CACCAGGCCC CAGCCCACAT
661 GCCTGCTTAA AGCCCTCTCC ATCCTCTGCC TCACCCAGTC CCCGCTGAGA CTGAGCAGAC
721 GCCTCCAGGA TCTGTCGGCA GagctCCTCG AGAAGcACTG ACCAACAGCG TGAAGACATT
781 ACATCCAGTG CACAGGTAAT TGAATTCTTG TTGGATTACT GTCTCTAAGG CCTGGGAGTT
841 TTATAATTGT TTCTTTATGT GTAATAATAA CCGTACTAGT CATTGTGATA CTTATTTGGG
901 AAGTAAGCCT TTAAAGAGGT TATTAAAAAG ATTTACTGAT TCCCACACCG GAAGTTGCTC
961 TTCTAGCCGT GAAAGAAGGA CGGCGTGGTC TTCAGTTTGC TATGTCCACT AACAATATGT
1021 CCGACCCACG AAGGCCCACC AAAGTTATAC TTGTGGCACA TATAAACCTT TGGAATCAAG
1081 GGACCTGATA CTGGATTATT AAATATTTAA GAGAATTTAT ATTTAATAAA CTTGATACTT
1141 CAGTGAGAAA GGACAAATCA TCAGTAGAAT GGTTTTGAGA TAGCTGATGA TTCTATTAAA
1201 AGTAGATATG TGTGTGGGTT AAATGACTTA AGCATACATC AAAAGACTGA GAAAGGCTCT
1261 TAACATTAAA AGATGGGCCt ttttttttCT TTTAGGTTAA AGGATTACAT ATTAAAAATA
1321 TTGTATAGAC TCTTTAAGGC CAAAGAAGTG TGCTCATTTC TCCCGATTAA AAGTTATGAG
1381 TAAGAATTGG GTATTTTGCC TTTTGGGGGT GGGAGAtttg aaacagggtt tctccttaca
1441 gctctggctt tcctggagca gtgaattgag cctggctcct ctgtaggagt aacgagttgc
1501 tttaaccact gacatctttc caccaccacc accaccCCTT ATGAATAGAC TAAATTTATT
1561 TAAAGATTGA TATTTTCTAG CTTAATTATT TGGAAAGCAA ATAAGCACAC AGAAAAGCCT
1621 CCTCCCCaaa aaaatataaa taaaaaaGTC TATCAGGACA TAGTATATGT CCCTTCTGAT
1681 TAATCATTGG TGGTGAAACA ACCCATTCAG GGTGTCTCTT CCATCACAGT GGAttttttt
1741 CATATTGTTA CATGGAGAAG TTAACTTAGT AAGAATTGAT GTGCTTCTGT GCCAGTGAGA
1801 TAGCTCTGCA GGTAAAAGGG AGACTGACCC CCAACTTGTA CTCTGGCCGT GGCAGTCATG
1861 TGCACACAAG TAAACTGAAT AAATATAAAG AGCAGATTTT AGAGTGTTTT GGCTTTAAAT
1921 TAATTCTATA TTCTAACTCT ATTTGCAGTT TGCATTGAGA CTCCTTGCAT TCCGTCAGAT
1981 ACACAAAGTg ctactaactt cagcctActA aaAcaggctg gagacgtTga ggagaaccct
2041 ggacctTATC CATAcGACGT TCCAGATTAC GCagaagacg ccaaaaacat aaagaaaggc
2101 ccggcgccat tctatccgct ggaagatgga accgctggag agcaactgca taaggctatg
2161 aagagatacg ccctggttcc tggaacaatt gcttttacag atgcacatat cgaggtggac
2221 atcacttacg ctgagtactt cgaaatgtcc gttcggttgg cagaagctat gaaacgatat
2281 gggctgaata caaatcacag aatcgtcgta tgcagtgaaa actctcttca attctttatg
2341 ccggtgttgg gcgcgttatt tatcggagtt gcagttgcgc ccgcgaacga catttataat
2401 gaacgtgaat tgctcaacag tatgggcatt tcgcagccta ccgtggtgtt cgtttccaaa
2461 aaggggttgc aaaaaatttt gaacgtgcaa aaaaagctcc caatcatcca aaaaattatt
2521 atcatggatt ctaaaacgga ttaccaggga tttcagtcga tgtacacgtt cgtcacatct
2581 catctacctc ccggttttaa tgaatacgat tttgtgccag agtccttcga tagggacaag
2641 acaattgcac tgatcatgaa ctcctctgga tctactggtc tgcctaaagg tgtcgctctg
2701 cctcatagaa ctgcctgcgt gagattctcg catgccagag atcctatttt tggcaatcaa
2761 atcattccgg atactgcgat tttaagtgtt gttccattcc atcacggttt tggaatgttt
2821 actacactcg gatatttgat atgtggattt cgagtcgtct taatgtatag atttgaagaa
2881 gagctgtttc tgaggagcct tcaggattac aagattcaaa gtgcgctgct ggtgccaacc
2941 ctattctcct tcttcgccaa aagcactctg attgacaaat acgatttatc taatttacac
3001 gaaattgctt ctggtggcgc tcccctctct aaggaagtcg gggaagcggt tgccaagagg
3061 ttccatctgc caggtatcag gcaaggatat gggctcactg agactacatc agctattctg
3121 attacacccg agggggatga taaaccgggc gcggtcggta aagttgttcc attttttgaa
3181 gcgaaggttg tggatctgga taccgggaaa acgctgggcg ttaatcaaag aggcgaactg
3241 tgtgtgagag gtcctatgat tatgtccggt tatgtaaaca atccggaagc gaccaacgcc
3301 ttgattgaca aggatggatg gctacattct ggagacatag cttactggga cgaagacgaa
3361 cacttcttca tcgttgaccg cctgaagtct ctgattaagt acaaaggcta tcaggtggct
3421 cccgctgaat tggaatccat cttgctccaa caccccaaca tcttcgacgc aggtgtcgca
3481 ggtcttcccg acgatgacgc cggtgaactt cccgccgccg ttgttgtttt ggagcacgga
3541 aagacgatga cggaaaaaga gatcgtggat tacgtcgcca gtcaagtaac aaccgcgaaa
3601 aagttgcgcg gaggagttgt gtttgtggac gaagtaccga aaggtcttac cggaaaactc
3661 gacgcaagaa aaatcagaga gatcctcata aaggccaaga agggcggaaa gatgccgtgt
3721 aaGTACCTCT AGAGTCGACC CGGGCGGCCT CGAGGACGGG GTGAACTACG CCTGAGGATC
3781 CGATCTTTTT CCCTCTGCCA AAAATTATGG GGACATCATG AAGCCCCTTG AGCATCTGAC
3841 TTCTGGCTAA TAAAGGAAAT TTATTTTCAT TGCAATAGTG TGTTGGAATT TTTTGTGTCT
3901 CTCACTCGGA AGCAATTCGT TGATCTGAAT TTCGACCACC CATAATACCC ATTACCCTGG
3961 TAGATAAGTA GCATGGCGGG TTAATCATTA ACTACAAGGA ACCCCTAGTG ATGGAGTTGG
4021 CCACTCCCTC TCTGCGCGCT CGCTCGCTCA CTGAGGCCGG GCGACCAAAG GTCGCCCGAC
4081 GCCCGGGCTT TGCCCGGGCG GCCTCAGTGA GCGAGCGAGC GCGCAGCCTT AATTAACCTA
4141 ATTCACTGGC CGTCGTTTTA CAACGTCGTG ACTGGGAAAA CCCTGGCGTT ACCCAACTTA
4201 ATCGCCTTGC AGCACATCCC CCTTTCGCCA GCTGGCGTAA TAGCGAAGAG GCCCGCACCG
4261 ATCGCCCTTC CCAACAGTTG CGCAGCCTGA ATGGCGAATG GGACGCGCCC TGTAGCGGCG
4321 CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC
4381 TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC
4441 GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG
4501 ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG
4561 TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG
4621 GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT
4681 CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT TTTAACAAAA
4741 TATTAACGCT TACAATTTAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG
4801 TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT
4861 GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT
4921 TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT
4981 AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG
5041 CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA
5101 AGTTCTGCTA TGTGGCGCGG TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG
5161 CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT
5221 TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC
5281 TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA
5341 CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT
5401 ACCAAACGAC GAGCGTGACA CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT
5461 ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC
5521 GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA
5581 TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG
5641 TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG
5701 AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA
5761 AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA
5821 GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA
5881 CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG
5941 CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA
6001 TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA
6061 TACTGTTCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC
6121 TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG
6181 TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC
6241 GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT
6301 ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC
6361 GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG
6421 GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG
6481 CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT
6541 GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA
6601 TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG
6661 CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCCAATA CGCAAACCGC CTCTCCCCGC
6721 GCGTTGGCCG ATTCATTAAT GCAGCTGGCA CGACAGGTTT CCCGACTGGA AAGCGGGCAG
6781 TGAGCGCAAC GCAATTAATG TGAGTTAGCT CACTCATTAG GCACCCCAGG CTTTACACTT
6841 TATGCTTCCG GCTCGTATGT TGTGTGGAAT TGTGAGCGGA TAACAATTTC ACACAGGAAA
6901 CAGCTATGAC CATGATTACG CCAGATTTAA TTAAGGCCTT AATTAGG
//
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pAAV-TNT4-Zfr-P2A-HA-luciferase质粒载体应用举例
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