pQD301

LOCUS       pQD301               8264 bp DNA     circular SYN 24-OCT-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8264)
  AUTHORS   Westlake University
  TITLE     Direct Submission
  JOURNAL   Exported Aug 15, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..8264
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     146..167
                     /label=olyl630
     primer_bind     147..189
                     /label=olyl254
     primer_bind     167..189
                     /label=olyl433
     misc_feature    173..1239
                     /label=P_EF1a
     primer_bind     173..197
                     /label=P_EF1a F
     primer_bind     1089..1109
                     /label=P-EF1a-SEQ-F
     primer_bind     complement(1210..1239)
                     /label=P_EF1a R
     primer_bind     complement(1222..1252)
                     /label=olyl434
     primer_bind     complement(1230..1270)
                     /label=olyl253
     primer_bind     1237..1287
                     /label=opqd701
     primer_bind     1237..1261
                     /label=OPQD435
     primer_bind     1255..1281
                     /label=opqd770
     primer_bind     1266..1287
                     /label=opqd862
     misc_feature    1268..3751
                     /label=PAX7FOXO1
     misc_feature    1268..2416
                     /label=PAX7
     primer_bind     complement(2397..2436)
                     /label=opqd703
     primer_bind     2397..2436
                     /label=opqd704
     primer_bind     complement(2397..2416)
                     /label=OPQD434
     misc_feature    2417..3751
                     /label=foxo1
     primer_bind     2417..2438
                     /label=OPQD435
     primer_bind     complement(3732..3793)
                     /label=oXP07-FLAG-PAX3-R
     primer_bind     complement(3732..3751)
                     /label=opqd436
     primer_bind     complement(3744..3763)
                     /label=opqd767
     primer_bind     complement(3749..3766)
                     /label=opqd543
     misc_feature    3752..3766
                     /label=GS
     misc_feature    3767..3784
                     /label=His
     primer_bind     complement(3776..3846)
                     /label=oXP08-FLAG-PAX3-R
     misc_feature    3785..3799
                     /label=GS-Linker
     misc_feature    3800..3823
                     /label=flag
     primer_bind     complement(3802..3826)
                     /label=opqd860
     primer_bind     complement(3809..3848)
                     /label=opqd705
     primer_bind     complement(3824..3846)
                     /label=OPQD434
     primer_bind     complement(3824..3846)
                     /label=opqd436
     terminator      3861..4088
                     /label=bGH polyA
     rep_origin      4134..4562
                     /label=f1 bacteriophage origin of replication
                     /label=f1 bacteriophage origin of replication; arrow _f1 
                     ori
     misc_feature    4576..4905
                     /label=P_SV40
                     /label=nonstandard type: promoter_const
     rep_origin      4756..4891
                     /label=SV40 ORI
     misc_feature    4808..4857
                     /label=P_SV40 TSS-region
     CDS             4972..5766
                     /label=Translation 2136-2930
     misc_feature    4972..5763
                     /label=Kan/neoR
                     /label=nonstandard type: resistance
     primer_bind     complement(6106..6126)
                     /label=M13-rev
     misc_binding    complement(6132..6154)
                     /label=LacO
     promoter        complement(6158..6188)
                     /label=lac promoter
     rep_origin      complement(6512..7097)
                     /direction=LEFT
                     /label=high-copy-number ColE1/pMB1/pBR322/pUC origin o
                     /label=high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     _ori
     CDS             complement(7268..8128)
                     /label=Translation 5292-4432
     misc_feature    complement(7271..8128)
                     /label=AmpR
                     /label=nonstandard type: resistance
     primer_bind     complement(7458..7467)
                     /label=opqd770
     promoter        complement(8129..8233)
                     /label=AmpR promoter
     primer_bind     8170..8198
                     /label=olyl231-amp-pro
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattggcta gcgggaaagt
      181 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      241 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      301 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      361 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      421 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      481 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      541 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      601 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      661 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      721 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      781 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      841 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      901 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      961 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
     1021 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1081 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1141 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1201 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gaattagctt ggtacaagct
     1261 tgccaccatg gcggcccttc ccggcacggt accgagaatg atgcggccgg ctccggggca
     1321 gaactacccc cgcacgggat tccctttgga agtgtccacc ccgcttggcc aaggccgggt
     1381 caatcagctg ggaggggtct tcatcaatgg gcgacccctg cctaaccaca tccgccacaa
     1441 gatagtggag atggcccacc atggcatccg gccctgtgtc atctcccgac agctgcgtgt
     1501 ctcccacggc tgcgtctcca agattctttg ccgctaccag gagaccgggt ccatccggcc
     1561 tggggccatc ggcggcagca agcccagaca ggtggcgact ccggatgtag agaaaaagat
     1621 tgaggagtac aagagggaaa acccaggcat gttcagctgg gagatccggg acaggctgct
     1681 gaaggatggg cactgtgacc gaagcactgt gccctcagtg agttcgatta gccgcgtgct
     1741 cagaatcaag ttcgggaaga aagaggagga ggatgaagcg gacaagaagg aggacgacgg
     1801 cgaaaagaag gccaaacaca gcatcgacgg catcctgggc gacaaaggga accggctgga
     1861 cgagggctcg gatgtggagt cggaacctga cctcccactg aagcgcaagc agcgacgcag
     1921 tcggaccaca ttcacggccg agcagctgga ggagctggag aaggcctttg agaggaccca
     1981 ctacccagac atatacaccc gcgaggagct ggcgcagagg accaagctga cagaggcgcg
     2041 tgtgcaggtc tggttcagta accgccgcgc ccgttggcgt aagcaggcag gagccaacca
     2101 gctggcggcg ttcaaccacc ttctgccagg aggcttcccg cccaccggca tgcccacgct
     2161 gcccccctac cagctgccgg actccaccta ccccaccacc accatctccc aagatggggg
     2221 cagcactgtg caccggcctc agcccctgcc accgtccacc atgcaccagg gcgggctggc
     2281 tgcagcggct gcagccgccg acaccagctc tgcctacgga gcccgccaca gcttctccag
     2341 ctactctgac agcttcatga atccggcggc gccctccaac cacatgaacc cggtcagcaa
     2401 cggcctgtct cctcagaatt caattcgtca taatctgtcc ctacacagca agttcattcg
     2461 tgtgcagaat gaaggaactg gaaaaagttc ttggtggatg ctcaatccag agggtggcaa
     2521 gagcgggaaa tctcctagga gaagagctgc atccatggac aacaacagta aatttgctaa
     2581 gagccgaagc cgagctgcca agaagaaagc atctctccag tctggccagg agggtgctgg
     2641 ggacagccct ggatcacagt tttccaaatg gcctgcaagc cctggctctc acagcaatga
     2701 tgactttgat aactggagta catttcgccc tcgaactagc tcaaatgcta gtactattag
     2761 tgggagactc tcacccatta tgaccgaaca ggatgatctt ggagaagggg atgtgcattc
     2821 tatggtgtac ccgccatctg ccgcaaagat ggcctctact ttacccagtc tgtctgagat
     2881 aagcaatccc gaaaacatgg aaaatctttt ggataatctc aaccttctct catcaccaac
     2941 atcattaact gtttcgaccc agtcctcacc tggcaccatg atgcagcaga cgccgtgcta
     3001 ctcgtttgcg ccaccaaaca ccagtttgaa ttcacccagc ccaaactacc aaaaatatac
     3061 atatggccaa tccagcatga gccctttgcc ccagatgcct atacaaacac ttcaggacaa
     3121 taagtcgagt tatggaggta tgagtcagta taactgtgcg cctggactct tgaaggagtt
     3181 gctgacttct gactctcctc cccataatga cattatgaca ccagttgatc ctggggtagc
     3241 ccagcccaac agccgggttc tgggccagaa cgtcatgatg ggccctaatt cggtcatgtc
     3301 aacctatggc agccaggcat ctcataacaa aatgatgaat cccagctccc atacccaccc
     3361 tggacatgct cagcagacat ctgcagttaa cgggcgtccc ctgccccaca cggtaagcac
     3421 catgccccac acctcgggta tgaaccgcct gacccaagtg aagacacctg tacaagtgcc
     3481 tctgccccac cccatgcaga tgagtgccct ggggggctac tcctccgtga gcagctgcaa
     3541 tggctatggc agaatgggcc ttctccacca ggagaagctc ccaagtgact tggatggcat
     3601 gttcattgag cgcttagact gtgacatgga atccatcatt cggaatgacc tcatggatgg
     3661 agatacattg gattttaact ttgacaatgt gttgcccaac caaagcttcc cacacagtgt
     3721 caagacaacg acacatagct gggtgtcagg cggtggcggc gggagtcatc atcaccatca
     3781 ccacggtggc ggtggcagcg attacaagga cgatgatgac aaataatcta gagggcccgt
     3841 ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
     3901 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
     3961 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
     4021 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg
     4081 ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc
     4141 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     4201 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     4261 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     4321 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     4381 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     4441 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     4501 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     4561 ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
     4621 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     4681 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
     4741 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     4801 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc
     4861 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct
     4921 tgtatatcca ttttcggatc tgatcaagag acaggatgag gatcgtttcg catgattgaa
     4981 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac
     5041 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg
     5101 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag
     5161 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt
     5221 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg
     5281 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg
     5341 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga
     5401 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag
     5461 gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat
     5521 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt
     5581 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg
     5641 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt
     5701 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc
     5761 ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac
     5821 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
     5881 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca
     5941 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     6001 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     6061 atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt
     6121 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     6181 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     6241 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     6301 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     6361 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     6421 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     6481 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     6541 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     6601 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     6661 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     6721 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     6781 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     6841 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     6901 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     6961 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     7021 ccggcaaaca aaccaccgct ggtagcggtt tttttgtttg caagcagcag attacgcgca
     7081 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     7141 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     7201 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     7261 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     7321 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     7381 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     7441 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     7501 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     7561 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     7621 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     7681 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     7741 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     7801 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     7861 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     7921 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     7981 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     8041 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     8101 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     8161 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     8221 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtc
//