| 质粒名称: | pAAV-EFS-DIO-GRAB_OTmut |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000156 |
| 产品用途: | Expresses the oxytocin-insensitive control sensor GRAB_OTmut in Cre positive cells |
| 存储实验室: | Yulong Li Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV |
| 表达系统: | 腺病毒，哺乳动物表达 |
| 启动子: | EFS |
| 拷贝数: | 低拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a，TOP10 |
|
插入基因信息 |
|
| 插入基因名称: | GRAB_OTmut |
| 物种来源: | Synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
买家导航
pAAV-EFS-DIO-GRAB_OTmut载体质粒基本信息
pAAV-EFS-DIO-GRAB_OTmut质粒图谱载体图谱和pAAV-EFS-DIO-GRAB_OTmut载体序列质粒序列多克隆位点信息
pAAV-EFS-DIO-GRAB_OTmut质粒载体简介
pAAV-EFS-DIO-GRAB_OTmut质粒序列载体序列
LOCUS pAAV-EFS-DIO-GRAB_OTmut 6462 bp DNA circular SYN 21-APR-2023
DEFINITION Expresses the genetically-encoded fluorescent oxytocin(OT) sensor
GRAB_OT1.0 in a cre-dependent manner.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6462)
TITLE oxytocin sensors
REFERENCE 2 (bases 1 to 6462)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 28, 2026 from SnapGene 6.0.2
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..6462
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 2..131
/label=AAV2 ITR (alternate)
/note="Functional equivalent of wild-type AAV2 ITR"
promoter 152..363
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
protein_bind 382..415
/label=lox2272
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GGATACTT). lox2272 sites are compatible with each
other, but incompatible with loxP or loxN sites (Lee and
Saito, 1988)."
protein_bind 466..499
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
CDS complement(510..2348)
/codon_start=1
/label=GRAB_OTmut
/translation="TTGEGAFAANWSAEAVNGSAAPPGTEGNRTAGPPQRNEALARVEV
AVLCLILFLALSGNACVLLALRTTRHKHSRLFFFMKHLSIADLVVAVFQVLPQLLWDIT
FRFYGPDLLCRLVKYLQVVGMFASTYLLLLMSLDRCLAICQPLRSLSRRTDRLAVLVTW
LGCLVASAPQVHIFSLREVADGVFDCWAVFIQPWGPKAYITWITLAVYIVPVIVLATCY
GLISFKIWQNLRLKTAGGNVYIKADKQKNGIKANFHIRHNIEDGGVQLAYHYQQNTPIG
DGPVLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVRK
GEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATEGKLTLKFICTTGKLPVPWPTLVT
TLTYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVN
RIELKGIDFKEDGNILGHKLEYNTGAAAKLISKAKIRTVKMTFIVVLAFIVCWTPFFFV
QMWSVWDADAPKEASPFIIAMLLASLNSCCNPWIYMLFTGHLFQELVQRFLCCSFRRLK
GSRPGETSVSKKSNSSTFVLSQYSSSQRRCSQPSTL"
primer_bind 1015..1034
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 1080..1101
/label=WKG155-F
primer_bind 1274..1295
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(1401..1422)
/label=EGFP-C
/note="EGFP, forward primer"
protein_bind complement(2348..2372)
/gene="mutant version of attB"
/label=attB1
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
sig_peptide complement(2373..2435)
/codon_start=1
/product="leader sequence from mouse immunoglobulin kappa
light chain"
/label=Ig-kappa leader
/translation="METDTLLLWVLLLWVPGSTGD"
regulatory 2432..2441
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
protein_bind complement(2448..2481)
/label=lox2272
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GGATACTT). lox2272 sites are compatible with each
other, but incompatible with loxP or loxN sites (Lee and
Saito, 1988)."
protein_bind complement(2532..2565)
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
misc_feature 2590..3178
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(2643..2663)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(3061..3072)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
polyA_signal 3210..3686
/label=hGH poly(A) signal
/note="human growth hormone polyadenylation signal"
primer_bind complement(3409..3428)
/label=hGH-PA-R
/note="Human growth hormone terminator, reverse primer"
repeat_region 3726..3866
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
repeat_region 3726..3855
/label=AAV2 ITR
rep_origin 3941..4396
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(4028..4047)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 4238..4259
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(4413..4432)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 4532..4554
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(4592..4610)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 4678..4782
/gene="bla"
/label=AmpR promoter
CDS 4783..5643
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(5001..5020)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 5814..6402
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 6303..6322
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 tcctgcaggc agctgcgcgc tcgctcgctc actgaggccg cccgggcgtc gggcgacctt
61 tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca actccatcac
121 taggggttcc tgcggccgca cgcgtaagct tgggcagagc gcacatcgcc cacagtcccc
181 gagaagttgg ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta
241 aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg
301 tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca
361 cagtctagag tcgactccgg aataacttcg tataggatac tttatacgaa gttatgcaga
421 atggtagctg gattgtagct gctattagca atatgaaacc tcttaataac ttcgtatagc
481 atacattata cgaagttatg gcgcatatgt cacagcgtgg atggctgcga gcatcttctc
541 tggctggagc tgtactggct caggacaaag gtagacgagt tgctcttttt gctgacgctt
601 gtctccccag gccggctgcc tttcaggcgg cggaatgagc agcagaggaa gcgctgcaca
661 agttcttgga agaggtggcc cgtgaagagc atgtagatcc aggggttgca gcagctgttg
721 aggctggcca ggagcatggc gatgatgaaa ggtgaggctt ccttgggcgc atcggcatcc
781 cagacactcc acatctgcac gaaaaagaat ggcgtccagc acacgatgaa ggccagcacg
841 acgatgaagg tcatcttgac cgtgcggatc ttggccttag agatgagctt tgctgctgct
901 ccggtgttgt actccagctt gtgccccagg atgttgccgt cctccttgaa gtcgatgccc
961 ttcagctcga tgcggttcac cagggtgtcg ccctcgaact tcacctcggc gcgggtcttg
1021 tagttgccgt cgtccttgaa gaagatggtg cgctcctgga tgtagccttc gggcatggcg
1081 gacttgaaga agtcgtgctg cttcatgtgg tcggggtagc ggctgaagca ctgcacgccg
1141 taggtcaggg tggtcacgag ggtgggccag ggcacgggca gcttgccggt ggtgcagatg
1201 aacttcaggg tcagcttgcc ctcggtggca tcgccctcac cctcgccgga cacgctgaac
1261 ttgtggccgt ttacgtcgcc gtccagctcg accaggatgg gcaccacccc ggtgaacagc
1321 tcctcgccct ttctcaccat gctccctccg gtaccgccct tgtacagctc gtccatgccg
1381 agagtgatcc cggcggcggt cacgaactcc agcaggacca tgtgatcgcg cttctcgttg
1441 gggtctttcg aaagtttgga ctgcacgctc aggtagtggt tgtcgggcag cagcacgggg
1501 ccgtcgccga tgggggtgtt ctgctggtag tggtaggcga gctgcacgcc gccgtcctcg
1561 atgttgtggc ggatgtggaa gttcgccttg atgccgttct tctgcttgtc ggccttgata
1621 tagacgtttc ctcccgccgt cttgagccgt aaattctgcc agatcttgaa gctgataagg
1681 ccatagcagg tggcaagcac gatgacgggc acaatgtaga cggcgagcgt gatccacgtg
1741 atgtaggcct tgggccccca gggttgaatg aaaacggccc agcagtcgaa gacaccgtcg
1801 gccacctcgc gcagcgagaa gatgtgcacc tgcggcgcgc tggccaccag gcagccgagc
1861 catgtgacga gtaccgccag gcggtcggtg cggcggctca gcgagcgcag cggctggcag
1921 atggccaggc agcggtcgag cgacatgagc agcagcaggt aggtggacgc gaacatgccc
1981 acaacctgca ggtacttgac gaggcggcac agcaggtcgg gcccgtagaa gcggaacgtg
2041 atgtcccaca gaagctgcgg cagcacctgg aacaccgcca ctaccaggtc ggctatgctc
2101 aggtgcttca tgaagaagaa gaggcgcgag tgcttgtggc gcgtggtgcg cagcgctagc
2161 agcacgcacg cgttgccgct cagcgccagg aacaggatga ggcacagcac ggccacctcc
2221 acccgcgcca gggcctcgtt gcgctgtggc ggcccggcag tgcgattgcc ctcggttccc
2281 ggcggcgccg cgctcccgtt gaccgcctca gcgctccagt tagccgcaaa cgcaccctca
2341 ccggtggtgc caactttttt gtacaaactt gtgtcaccag tggaacctgg aacccagagc
2401 agcagtaccc atagcaggag tgtgtctgtc tccatggtgg cgctagcata acttcgtata
2461 aagtatccta tacgaagtta tttgccttaa cccagaaatt atcactgtta ttctttagaa
2521 tggtgcaaag aataacttcg tataatgtat gctatacgaa gttatgaatt cgatatcaag
2581 cttatcgata atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
2641 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
2701 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
2761 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
2821 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
2881 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
2941 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct
3001 tggctgctcg cctatgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
3061 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
3121 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat
3181 cgataccgag cgctgctcga gagatctacg ggtggcatcc ctgtgacccc tccccagtgc
3241 ctctcctggc cctggaagtt gccactccag tgcccaccag ccttgtccta ataaaattaa
3301 gttgcatcat tttgtctgac taggtgtcct tctataatat tatggggtgg aggggggtgg
3361 tatggagcaa ggggcaagtt gggaagacaa cctgtagggc ctgcggggtc tattgggaac
3421 caagctggag tgcagtggca caatcttggc tcactgcaat ctccgcctcc tgggttcaag
3481 cgattctcct gcctcagcct cccgagttgt tgggattcca ggcatgcatg accaggctca
3541 gctaattttt gtttttttgg tagagacggg gtttcaccat attggccagg ctggtctcca
3601 actcctaatc tcaggtgatc tacccacctt ggcctcccaa attgctggga ttacaggcgt
3661 gaaccactgc tcccttccct gtccttctga ttttgtaggt aaccacgtgc ggaccgagcg
3721 gccgcaggaa cccctagtga tggagttggc cactccctct ctgcgcgctc gctcgctcac
3781 tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag
3841 cgagcgagcg cgcagctgcc tgcaggggcg cctgatgcgg tattttctcc ttacgcatct
3901 gtgcggtatt tcacaccgca tacgtcaaag caaccatagt acgcgccctg tagcggcgca
3961 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcctta
4021 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
4081 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
4141 cccaaaaaac ttgatttggg tgatggttca cgtagtgggc catcgccctg atagacggtt
4201 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
4261 acaacactca actctatctc gggctattct tttgatttat aagggatttt gccgatttcg
4321 gtctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
4381 ttaacgttta caattttatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
4441 agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg
4501 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca
4561 ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt
4621 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
4681 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
4741 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
4801 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
4861 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
4921 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
4981 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
5041 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
5101 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
5161 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
5221 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
5281 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
5341 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
5401 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
5461 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
5521 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
5581 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
5641 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
5701 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
5761 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
5821 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
5881 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
5941 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
6001 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
6061 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
6121 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
6181 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
6241 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
6301 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
6361 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
6421 tttttacggt tcctggcctt ttgctggcct tttgctcaca tg
//
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