| 质粒名称: | DT480 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 80413 |
| 存储实验室: | Thomas Cooper |
|
载体骨架基本信息 |
|
| 载体名称: | sp72 |
| 载体出品公司: | Clontech |
| 空载体大小: | 2400 bp |
| 完整质粒大小: | 7017 bp |
| 载体类型: | 哺乳细胞表达 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | human DMPK exons 11-15 with 480 interrupted CTG repeats |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 6240 bp |
|
文献引用方法 |
|
| 材料和方法部分: | DT480 was a gift from Thomas Cooper (Addgene plasmid # 80413 ; http://n2t.net/addgene:80413 ; RRID:Addgene_80413) |
| 参考文献部分: | Muscleblind proteins regulate alternative splicing. Ho TH, Charlet-B N, Poulos MG, Singh G, Swanson MS, Cooper TA. EMBO J. 2004 Aug 4;23(15):3103-12. Epub 2004 Jul 15. 10.1038/sj.emboj.7600300 PubMed 15257297 |
买家导航
DT480载体质粒基本信息
DT480质粒图谱载体图谱和DT480载体序列质粒序列多克隆位点信息
DT480质粒载体简介
DT480质粒序列载体序列
LOCUS DT480 7017 bp DNA circular SYN 14-APR-2025
DEFINITION Expresses 480 interrupted CUG repeats in the context of human DMPK
genomic segment containing exons 11-15 with repeats inserted at site
in exon 15 that contains the repeats.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7017)
AUTHORS Ho TH, Charlet-B N, Poulos MG, Singh G, Swanson MS, Cooper TA
TITLE Muscleblind proteins regulate alternative splicing.
JOURNAL EMBO J. 2004 Aug 4;23(15):3103-12. Epub 2004 Jul 15.
PUBMED 15257297
REFERENCE 2 (bases 1 to 7017)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 14, 2025 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J.";
date: "2004-08-4"; volume: "23(15)"; pages: "3103-12. Epub 2004 Jul
15"
COMMENT Sequence Label: DT480
FEATURES Location/Qualifiers
source 1..7017
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 21..40
/label=T7
/note="T7 promoter, forward primer"
promoter 21..39
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
enhancer 139..442
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 443..646
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 597..617
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
promoter complement(4692..4710)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(4693..4710)
/label=SP6
/note="SP6 promoter, forward primer"
primer_bind complement(4791..4810)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 4910..4932
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(4970..4988)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 5056..5160
/gene="bla"
/label=AmpR promoter
CDS join(5161..5229,5230..6021)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAISMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(5379..5398)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 6192..6780
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 6934..6951
/label=L4440
/note="L4440 vector, forward primer"
ORIGIN
1 ttaacctggc ttatcgaaat taatacgact cactataggg agaccggcag atctgatatc
61 atcgatgaat tcgagctcgg taccctaata gtaatcaatt acggggtcat tagttcatag
121 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
181 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
241 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
301 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
361 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
421 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata
481 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
541 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca
601 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctggtt tagtgaaccg
661 tcagatccgc tagcgctacc ggactcagat caggctgagg ccgaggtgac gctgcgggag
721 ctccaggaag ccctggagga ggaggtgctc acccggcaga gcctgagccg ggagatggag
781 gccatccgca cggacaacca gaacttcgcc aggtcgggat cggggccggg gccggggccg
841 ggatgcgggc cggtggcaac ccttggcagc ccctctcgtc cggcccggac ggactcaccg
901 tccttacctc cccacagtca actacgcgag gcagaggctc ggaaccggga cctagaggca
961 cacgtccggc agttgcagga gcggatggag ttgctgcagg cagagggagc cacaggtgag
1021 tccctcatgt gtccccttcc ccggaggacc gggaggaggt gggccgtctg ctccgcgggg
1081 cgtgtataga cacctggagg agggaaggga cccacgctgg ggcacgccgc gccaccgccc
1141 tccttcgccc ctccacgcgc cctatgcctc tttcttctcc ttccagctgt cacgggggtc
1201 cccagtcccc gggccacgga tccaccttcc catgtaagac ccctctcttt cccctgcctc
1261 agacctgctg cccattctgc agatcccctc cctggctcct ggtctccccg tccagatata
1321 gggctcaccc tacgtctttg cgactttaga gggcagaagc cctttattca gccccagatc
1381 tccctccgtt caggcctcac cagattccct ccgggatctc cctagataac ctccccaacc
1441 tcgattccgc tcgctgtctc tcgccccacc gctgagggct gggctgggct ccgatcgggt
1501 cacctgtccc ttctctctcc agctagatgg ccccccggcc gtggctgtgg gccagtgccc
1561 gctggtgggg ccaggcccca tgcaccgccg ccacctgctg ctccctgcca gggtacgtcc
1621 ggctgcccac gcccccctcc gccgtcgcgc cccgcgctcc acccgccccg tgccacccgc
1681 ttagctgcgc atttgcgggg ctgggcccac ggcaggaggg cggatcttcg ggcagccaat
1741 caacacaggc cgctaggaag cagccaatga cgagttcgga cgggattcga ggcgtgcgag
1801 tggactaaca acagctgtag gctgttgggg cgggggcggg gcgcagggaa gagtgcgggc
1861 ccacctatgg gcgtaggcgg ggcgagtccc aggagccaat cagaggccca tgccgggtgt
1921 tgacctcgcc ctctccccgc aggtccctag gcctggccta tcggaggcgc tttccctgct
1981 cctgttcgcc gttgttctgt ctcgtgccgc cgccctgggc tgcattgggt tggtggccca
2041 cgccggccaa ctcaccgcag tctggcgccg cccaggagcc gcccgcgctc cctgaaccct
2101 agaactgtct tcgactccgg ggccccgttg gaagactgag tgcccggggc acggcacaga
2161 agccgcgccc accgcctgcc agttcacaac cgctccgagc gtgggtctcc gcccagctcc
2221 agtcctgtga tccgggcccg ccccctagcg gccggggagg gaggggccgg gtccgcggcc
2281 ggcgaacggg gctcgaaggg tccttgtagc cgggaatgtc gactgctgct gctgctgctg
2341 ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc tgctcgactg ctgctgctgc
2401 tgctgctgct gctgctgctg ctgctgctgc tgctgctgct gctgctgctc gactgctgct
2461 gctgctgctg ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc tgctcgactg
2521 ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc tgctgctgct gctgctgctc
2581 gactgctgct gctgctgctg ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc
2641 tgctcgactg ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc tgctgctgct
2701 gctgctgctc gactgctgct gctgctgctg ctgctgctgc tgctgctgct gctgctgctg
2761 ctgctgctgc tgctcgactg ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc
2821 tgctgctgct gctgctgctc gactgctgct gctgctgctg ctgctgctgc tgctgctgct
2881 gctgctgctg ctgctgctgc tgctcgactg ctgctgctgc tgctgctgct gctgctgctg
2941 ctgctgctgc tgctgctgct gctgctgctc gactgctgct gctgctgctg ctgctgctgc
3001 tgctgctgct gctgctgctg ctgctgctgc tgctcgactg ctgctgctgc tgctgctgct
3061 gctgctgctg ctgctgctgc tgctgctgct gctgctgctc gactgctgct gctgctgctg
3121 ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc tgctcgactg ctgctgctgc
3181 tgctgctgct gctgctgctg ctgctgctgc tgctgctgct gctgctgctc gactgctgct
3241 gctgctgctg ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc tgctcgactg
3301 ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc tgctgctgct gctgctgctc
3361 gactgctgct gctgctgctg ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc
3421 tgctcgactg ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc tgctgctgct
3481 gctgctgctc gactgctgct gctgctgctg ctgctgctgc tgctgctgct gctgctgctg
3541 ctgctgctgc tgctcgactg ctgctgctgc tgctgctgct gctgctgctg ctgctgctgc
3601 tgctgctgct gctgctgctc gactgctgct gctgctgctg ctgctgctgc tgctgctgct
3661 gctgctgctg ctgctgctgc tgctcgactg ctgctgctgc tgctgctgct gctgctgctg
3721 ctgctgctgc tgctgctgct gctgctgctc gactgctgct gctgctgctg ctgctgctgc
3781 tgctgctgct gctgctgctg ctgctgctgc tgctcgactg ctgctgctgc tgctgctgct
3841 gctgctgctg ctgctgctgc tgctgctgct gctgctgctc gatcgacggg ggatcacaga
3901 ccatttcttt ctttcggcca ggctgaggcc ctgacgtgga tgggcaaact gcaggcctgg
3961 gaaggcagca agccgggccg tccgtgttcc atcctccacg cacccccacc tatcgttggt
4021 tcgcaaagtg caaagctttc ttgtgcatga cgccctgctc tggggagcgt ctggcgcgat
4081 ctctgcctgc ttactcggga aatttgcttt tgccaaaccc gctttttcgg ggatcccgcg
4141 cccccctcct cacttgcgct gctctcggag ccccagccgg ctccgcccgc ttcggcggtt
4201 tggatattta ttgacctcgt cctccgactc gctgacaggc tacaggaccc ccaacaaccc
4261 caatccacgt tttggatgca ctgagacccc gacattcctc ggtatttatt gtctgtcccc
4321 acctaggacc cccacccccg accctcgcga ataaaaggcc ctccatctgc ccaaagctct
4381 ggactccaca gtgtccgcgg tttgcgttgt gggccggagc tccgcagcgg gccaatccgg
4441 aggcgtgtgg aggcggccga aggtctggga ggagctagcg ggatgcgaag cggccgaatc
4501 agggttgggg gaggaaaagc cacggggcgg ggctttggcg tccggccaat aggagggcga
4561 gcgggccacc cggaggcacc gcccccgccc agctgtggcc cagctgtgcc accgagcgtc
4621 gagaagaggg ggctgggctg gcagcgcgcg cggccatcct ccttccactg cgcctgcgca
4681 cttaatcgag ttctatagtg tcacctaaat cgtatgtgta tgatacataa ggttatgtat
4741 taattgtagc cgcgttctaa cgacaatatg tccatatggt gcactctcag tacaatctgc
4801 tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga
4861 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
4921 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata
4981 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
5041 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
5101 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
5161 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
5221 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
5281 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
5341 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
5401 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
5461 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
5521 tgcagtgctg ccataagcat gagtgataac actgcggcca acttacttct gacaacgatc
5581 ggaggaccga aggagctaac cgcttttttt cacaacatgg gggatcatgt aactcgcctt
5641 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
5701 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
5761 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
5821 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
5881 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
5941 acgacgggca gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
6001 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
6061 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
6121 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
6181 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
6241 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
6301 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
6361 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
6421 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
6481 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
6541 gagcgaacga cctacaccga actgagatac ctacagcgtg agcattgaga aagcgccacg
6601 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
6661 cgcacgaggg agcttccagg ggggaacgcc tggtatcttt atagtcctgt cgggtttcgc
6721 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggccgag cctatggaaa
6781 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
6841 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
6901 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
6961 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagg
//
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