| 质粒名称: | pSaCas9_GFP |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 64709 |
| 存储实验室: | Kiran Musunuru |
|
载体骨架基本信息 |
|
| 载体名称: | pCAG |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 4345 bp |
| 完整质粒大小: | 8398 bp |
| 载体类型: | 哺乳细胞表达,CRISPR |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | SaCas9-2A-GFP,Staphylococcus aureus Cas9 |
| 物种来源: | 合成 |
| 插入大小: | 4053 bp |
| 启动子 | CAG |
| 标签/融合蛋白: |
3xFLAG（插入N端子）
SV40 NLS（N端子插入）
2A-GFP（插入件上的C端子）
|
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | EcoRI(未破坏) |
| 3' 克隆位点: | PstI (未破坏) |
| 5' 测序引物 | ggctctagtgcctctgctaacc |
| 3' 测序引物 | M13R |
|
文献引用方法 |
|
| 材料和方法部分: | pSaCas9_GFP was a gift from Kiran Musunuru (Addgene plasmid # 64709 ; http://n2t.net/addgene:64709 ; RRID:Addgene_64709) |
买家导航
pSaCas9_GFP载体 pSaCas9GFP质粒基本信息
pSaCas9_GFP质粒图谱 pSaCas9GFP载体图谱和pSaCas9_GFP载体序列 pSaCas9GFP质粒序列多克隆位点信息
pSaCas9_GFP质粒 pSaCas9GFP载体简介
pSaCas9_GFP质粒序列 pSaCas9GFP载体序列
LOCUS pSaCas9_GFP 8398 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Co-expression of human codon-optimized Staphylococcus aureus Cas9
nuclease and GFP, plasmid optimized for expression in human
pluripotent stem cells and other mammalian cells.
ACCESSION .
VERSION .
KEYWORDS pSaCas9_GFP
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8398)
TITLE Staphylococcus aureus Cas9
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 8398)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8398
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 8..387
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 390..665
/label=chicken beta-actin promoter
intron 666..1678
/label=chimeric intron
/note="chimera between introns from chicken beta-actin and
rabbit beta-globin"
primer_bind 1602..1624
/label=pCAGGS-5
/note="Chimeric intron in CAG promoter, forward primer"
primer_bind 1686..1705
/label=pCAG-F
/note="Rabbit beta-globin intron, for pCAG plasmids,
forward primer"
regulatory 1739..1748
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 1748..1813
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
CDS 1820..1840
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 1850..5008
/codon_start=1
/product="Cas9 endonuclease from the Staphylococcus aureus
Type II CRISPR/Cas system"
/label=SaCas9
/note="generates RNA-guided double strand breaks in DNA"
/translation="MKRNYILGLDIGITSVGYGIIDYETRDVIDAGVRLFKEANVENNE
GRRSKRGARRLKRRRRHRIQRVKKLLFDYNLLTDHSELSGINPYEARVKGLSQKLSEEE
FSAALLHLAKRRGVHNVNEVEEDTGNELSTKEQISRNSKALEEKYVAELQLERLKKDGE
VRGSINRFKTSDYVKEAKQLLKVQKAYHQLDQSFIDTYIDLLETRRTYYEGPGEGSPFG
WKDIKEWYEMLMGHCTYFPEELRSVKYAYNADLYNALNDLNNLVITRDENEKLEYYEKF
QIIENVFKQKKKPTLKQIAKEILVNEEDIKGYRVTSTGKPEFTNLKVYHDIKDITARKE
IIENAELLDQIAKILTIYQSSEDIQEELTNLNSELTQEEIEQISNLKGYTGTHNLSLKA
INLILDELWHTNDNQIAIFNRLKLVPKKVDLSQQKEIPTTLVDDFILSPVVKRSFIQSI
KVINAIIKKYGLPNDIIIELAREKNSKDAQKMINEMQKRNRQTNERIEEIIRTTGKENA
KYLIEKIKLHDMQEGKCLYSLEAIPLEDLLNNPFNYEVDHIIPRSVSFDNSFNNKVLVK
QEENSKKGNRTPFQYLSSSDSKISYETFKKHILNLAKGKGRISKTKKEYLLEERDINRF
SVQKDFINRNLVDTRYATRGLMNLLRSYFRVNNLDVKVKSINGGFTSFLRRKWKFKKER
NKGYKHHAEDALIIANADFIFKEWKKLDKAKKVMENQMFEEKQAESMPEIETEQEYKEI
FITPHQIKHIKDFKDYKYSHRVDKKPNRELINDTLYSTRKDDKGNTLIVNNLNGLYDKD
NDKLKKLINKSPEKLLMYHHDPQTYQKLKLIMEQYGDEKNPLYKYYEETGNYLTKYSKK
DNGPVIKKIKYYGNKLNAHLDITDDYPNSRNKVVKLSLKPYRFDVYLDNGVYKFVTVKN
LDVIKKENYYEVNSKCYEEAKKLKKISNQAEFIASFYNNDLIKINGELYRVIGVNNDLL
NRIEVNMIDITYREYLENMNDKRPPRIIKTIASKTQSIKKYSTDILGNLYEVKSKKHPQ
IIKKG"
CDS 5024..5077
/codon_start=1
/product="2A peptide from Thosea asigna virus capsid
protein"
/label=T2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="EGRGSLLTCGDVEENPGP"
CDS 5078..5797
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(5123..5144)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(5384..5403)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 5731..5752
/label=EGFP-C
/note="EGFP, forward primer"
polyA_signal 5840..5961
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(5877..5896)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 5931..5950
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(6010..6026)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(6010..6026)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(6023..6045)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 6034..6050
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(6058..6088)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 6103..6124
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(6241..6258)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(6412..7000)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(6492..6511)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(7171..8031)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 7794..7813
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(8032..8136)
/gene="bla"
/label=AmpR promoter
primer_bind complement(8215..8234)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
ORIGIN
1 acgcgttgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
61 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
121 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
181 atagggactt tccattgacg tcaatgggtg gactatttac ggtaaactgc ccacttggca
241 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
301 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
361 tacgtattag tcatcgctat taccatgggt cgaggtgagc cccacgttct gcttcactct
421 ccccatctcc cccccctccc cacccccaat tttgtattta tttatttttt aattattttg
481 tgcagcgatg ggggcggggg gggggggggc gcgcgccagg cggggcgggg cggggcgagg
541 ggcggggcgg ggcgaggcgg agaggtgcgg cggcagccaa tcagagcggc gcgctccgaa
601 agtttccttt tatggcgagg cggcggcggc ggcggcccta taaaaagcga agcgcgcggc
661 gggcgggagt cgctgcgttg ccttcgcccc gtgccccgct ccgcgccgcc tcgcgccgcc
721 cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc
781 ctccgggctg taattagcgc ttggtttaat gacggctcgt ttcttttctg tggctgcgtg
841 aaagccttaa agggctccgg gagggccctt tgtgcggggg ggagcggctc ggggggtgcg
901 tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggcccgc gctgcccggc ggctgtgagc
961 gctgcgggcg cggcgcgggg ctttgtgcgc tccgcgtgtg cgcgagggga gcgcggccgg
1021 gggcggtgcc ccgcggtgcg ggggggctgc gaggggaaca aaggctgcgt gcggggtgtg
1081 tgcgtggggg ggtgagcagg gggtgtgggc gcggcggtcg ggctgtaacc cccccctgca
1141 cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg
1201 tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc
1261 ggggccgcct cgggccgggg agggctcggg ggaggggcgc ggcggccccc ggagcgccgg
1321 cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt gcgagagggc
1381 gcagggactt cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac
1441 cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga
1501 gggccttcgt gcgtcgccgc gccgccgtcc ccttctccat ctccagcctc ggggctgtcc
1561 gcagggggac ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt
1621 gaccggcggc tctagtgcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct
1681 cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat tcgcggccgc
1741 caccatggac tataaggacc acgacggaga ctacaaggat catgatattg attacaaaga
1801 cgatgacgat aagatggccc cgaagaaaaa gcgcaaggtc gaagcgtcca tgaaaaggaa
1861 ctacattctg gggctggaca tcgggattac aagcgtgggg tatgggatta ttgactatga
1921 aacaagggac gtgatcgacg caggcgtcag actgttcaag gaggccaacg tggaaaacaa
1981 tgagggacgg agaagcaaga ggggagccag gcgcctgaaa cgacggagaa ggcacagaat
2041 ccagagggtg aagaaactgc tgttcgatta caacctgctg accgaccatt ctgagctgag
2101 tggaattaat ccttatgaag ccagggtgaa aggcctgagt cagaagctgt cagaggaaga
2161 gttttccgca gctctgctgc acctggctaa gcgccgagga gtgcataacg tcaatgaggt
2221 ggaagaggac accggcaacg agctgtctac aaaggaacag atctcacgca atagcaaagc
2281 tctggaagag aagtatgtcg cagagctaca gctggaacgg ctgaagaaag atggcgaggt
2341 gagagggtca attaataggt tcaagacaag cgactacgtc aaagaagcca agcagctgct
2401 gaaagtgcag aaggcttacc accagctgga tcagagcttc atcgatactt atatcgacct
2461 gctggagact cggagaacct actatgaggg accaggagaa gggagcccct tcggatggaa
2521 agacatcaag gaatggtacg agatgctgat gggacattgc acctattttc cagaagagct
2581 gagaagcgtc aagtacgctt ataacgcaga tctgtacaac gccctgaatg acctgaacaa
2641 cctggtcatc accagggatg aaaacgagaa actggaatac tatgagaagt tccagatcat
2701 cgaaaacgtg tttaagcaga agaaaaagcc tacactgaaa cagattgcta aggagatcct
2761 ggtcaacgaa gaggacatca agggctaccg ggtgacaagc actggaaaac cagagttcac
2821 caatctgaaa gtgtatcacg atattaagga catcacagca cggaaagaaa tcattgagaa
2881 cgccgaactg ctggatcaga ttgctaagat cctgactatc taccagagtt ccgaggacat
2941 ccaggaagag ctgactaacc tgaacagcga gctgacccag gaagagatcg aacagattag
3001 taatctgaag gggtacaccg gaacacacaa cctgtccctg aaagctatca atctgattct
3061 ggatgagctg tggcatacaa acgacaatca gattgcaatc tttaaccggc tgaagctggt
3121 accaaaaaag gtggacctga gtcagcagaa agagatccca accacactgg tggacgattt
3181 cattctgtca cccgtggtca agcggagctt catccagagc atcaaagtga tcaacgccat
3241 catcaagaag tacggcctgc ccaatgatat cattatcgag ctggctaggg agaagaacag
3301 caaggacgca cagaagatga tcaatgagat gcagaaacga aaccggcaga ccaatgaacg
3361 cattgaagag attatccgaa ctaccgggaa agagaacgca aagtacctga ttgaaaaaat
3421 caagctgcac gatatgcagg agggaaagtg tctgtattct ctggaggcca tccccctgga
3481 ggacctgctg aacaatccat tcaactacga ggtcgatcat attatcccca gaagcgtgtc
3541 cttcgacaat tcctttaaca acaaggtgct ggtcaagcag gaagagaact ctaaaaaggg
3601 caataggact cctttccagt acctgtctag ttcagattcc aagatctctt acgaaacctt
3661 taaaaagcac attctgaatc tggccaaagg aaagggccgc atcagcaaga ccaaaaagga
3721 gtacctgctg gaagagcggg acatcaacag attctccgtc cagaaggatt ttattaaccg
3781 gaatctggtg gacacaagat acgctactcg cggcctgatg aatctgctgc gatcctattt
3841 ccgggtgaac aatctggatg tgaaagtcaa gtccatcaac ggcgggttca catcttttct
3901 gaggcgcaaa tggaagttta aaaaggagcg caacaaaggg tacaagcacc atgccgaaga
3961 tgctctgatt atcgcaaatg ccgacttcat ctttaaggag tggaaaaagc tggacaaagc
4021 caagaaagtg atggagaacc agatgttcga agagaagcag gccgaatcta tgcccgaaat
4081 cgagacagaa caggagtaca aggagatttt catcactcct caccagatca agcatatcaa
4141 ggatttcaag gactacaagt actctcaccg ggtggataaa aagcccaaca gagagctgat
4201 caatgacacc ctgtatagta caagaaaaga cgataagggg aataccctga ttgtgaacaa
4261 tctgaacgga ctgtacgaca aagataatga caagctgaaa aagctgatca acaaaagtcc
4321 cgagaagctg ctgatgtacc accatgatcc tcagacatat cagaaactga agctgattat
4381 ggagcagtac ggcgacgaga agaacccact gtataagtac tatgaagaga ctgggaacta
4441 cctgaccaag tatagcaaaa aggataatgg ccccgtgatc aagaagatca agtactatgg
4501 gaacaagctg aatgcccatc tggacatcac agacgattac cctaacagtc gcaacaaggt
4561 ggtcaagctg tcactgaagc catacagatt cgatgtctat ctggacaacg gcgtgtataa
4621 atttgtgact gtcaagaatc tggatgtcat caaaaaggag aactactatg aagtgaatag
4681 caagtgctac gaagaggcta aaaagctgaa aaagattagc aaccaggcag agttcatcgc
4741 ctccttttac aacaacgacc tgattaagat caatggcgaa ctgtataggg tcatcggggt
4801 gaacaatgat ctgctgaacc gcattgaagt gaatatgatt gacatcactt accgagagta
4861 tctggaaaac atgaatgata agcgcccccc tcgaattatc aaaacaatcg cctctaagac
4921 tcagagtatc aaaaagtact caaccgacat tctgggaaac ctgtatgagg tgaagagcaa
4981 aaagcaccct cagattatca aaaagggcag gtccggcggc ggagagggca gaggaagtct
5041 tctaacatgc ggtgacgtgg aggagaatcc cggcccaatg gtgagcaagg gcgaggagct
5101 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
5161 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
5221 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg
5281 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
5341 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
5401 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
5461 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
5521 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
5581 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
5641 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct
5701 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
5761 cgggatcact ctcggcatgg acgagctgta caagtaactg cagcgcgggg atctcatgct
5821 ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa
5881 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
5941 caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc
6001 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
6061 catacgagcc ggaagcataa agtgtaaagc ctagggtgcc taatgagtga gctaactcac
6121 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
6181 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
6241 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
6301 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
6361 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
6421 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
6481 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
6541 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
6601 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
6661 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
6721 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
6781 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
6841 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
6901 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
6961 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
7021 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
7081 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
7141 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
7201 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
7261 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
7321 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
7381 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
7441 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
7501 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
7561 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
7621 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
7681 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
7741 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
7801 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
7861 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
7921 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
7981 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
8041 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
8101 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
8161 tgacgtcgac ggatcgggag atcgatctcc cgatccccta gggtcgactc tcagtacaat
8221 ctgctctgat gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc
8281 tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat
8341 gaagaatctg cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatat
//
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pSaCas9_GFP质粒 pSaCas9GFP载体应用举例
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