| 质粒名称: | pYK521 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000825 |
| 产品用途: | Mammalian PhCMV-driven constitutive expression vector for 3xFLAG-tagged ZFP1-NLS-4xANR (PhCMV-ZFP1-3xFLAG-NLS-4xANR-pA). |
| 存储实验室: | Anping ZENG Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pCMV |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CMV |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | 3xFLAG-ZFP1-NLS-4xANR |
| 物种来源: | synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Programmable solid-state condensates for spatiotemporal control of mammalian gene expression
Wang Y., Jiang J., Xiong Q., Li S., Shao J., Xie M., Zeng A.P.
Nat. Chem. Biol. 2025 1–10 (2025)
|
pYK521
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pYK521载体质粒基本信息
pYK521质粒图谱载体图谱和pYK521载体序列质粒序列多克隆位点信息
pYK521质粒载体简介
pYK521质粒序列载体序列
LOCUS pYK521 6624 bp DNA circular SYN 11-FEB-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6624)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jul 28, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6624
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 232..819
/label=CMV
primer_bind 863..882
/label=T7
primer_bind 881..900
/label=oYK06-ANR-mEGFP-F
primer_bind 881..900
/label=oYK06-ANR-mEGFP-F
primer_bind 903..921
/label=oYK650_ZF_F
CDS 904..1431
/codon_start=1
/label=ZF1
/translation="SRPGERPFQCRICMRNFSEEANLRRHTRTHTGEKPFQCRICMRNF
SDHSSLKRHLRTHTGSQKPFQCRICMRNFSQSANLLRHTRTHTGEKPFQCRICMRNFSD
PSSLKRHLRTHTGSQKPFQCRICMRNFSQQTNLTRHTRTHTGEKPFQCRICMRNFSDAT
QLVRHLRTHLRGS"
primer_bind 1436..1459
/label=oYK563_Flag_F
CDS 1441..1506
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
primer_bind complement(1481..1544)
/label=oYK502_ZF_R
CDS 1513..1533
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
primer_bind 1535..1556
/label=oSCL1192
primer_bind 1535..1556
/label=oSCL1129
primer_bind 1535..1554
/label=oLZ147 NS3a F
primer_bind 1535..1553
/label=oYF0033
misc_feature 1537..1572
/label=linker
primer_bind 1537..1566
/label=oSCL388
misc_feature 1537..1557
/label=linker
primer_bind 1537..1556
/label=oLZ048 NSP3-F
primer_bind 1537..1552
/label=oYK28-mCherry-NS3a-F
primer_bind complement(1541..1556)
/label=oYK520_GS_R
primer_bind complement(1547..1572)
/label=oSCL1119
primer_bind complement(1547..1566)
/label=oSCL075
primer_bind complement(1550..1566)
/label=oYK472_NLS_R
primer_bind complement(1551..1586)
/label=oYK92_L7Ae_R
primer_bind complement(1555..1573)
/label=oYK75_NLS_mcp_R
primer_bind complement(1561..1615)
/label=oSCL388
CDS 1567..1641
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
primer_bind complement(1567..1612)
/label=oYK215_Mcp_B_R
primer_bind complement(1567..1586)
/label=oYK195_ANR_R
primer_bind 1567..1581
/label=oYK82_LinkANR_F
primer_bind complement(1568..1587)
/label=test2
primer_bind 1593..1612
/label=oYK215_ANR1rep_F
primer_bind complement(1593..1612)
/label=oYK215_ANR1rep_R
primer_bind 1622..1641
/label=test1
primer_bind complement(1625..1641)
/label=TEST3
CDS 1642..1704
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
primer_bind 1642..1657
/label=Primer 4
primer_bind complement(1681..1724)
/label=oYK195_ANR_R
primer_bind complement(1686..1704)
/label=oYK82_LinkANR_R
primer_bind 1689..1703
/label=Primer 3
primer_bind complement(1700..1750)
/label=oYK215_Mcp_B_R
CDS 1705..1779
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
primer_bind complement(1705..1724)
/label=oYK92_L7Ae_R
primer_bind 1705..1719
/label=oYK82_LinkANR_F
primer_bind complement(1706..1725)
/label=test2
primer_bind 1731..1750
/label=oYK215_ANR1rep_F
primer_bind complement(1731..1750)
/label=oYK215_ANR1rep_R
primer_bind 1760..1779
/label=test1
primer_bind complement(1763..1779)
/label=TEST3
CDS 1780..1842
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
primer_bind 1780..1795
/label=Primer 4
primer_bind complement(1819..1862)
/label=oYK195_ANR_R
primer_bind complement(1824..1842)
/label=oYK82_LinkANR_R
primer_bind 1827..1841
/label=Primer 3
primer_bind complement(1838..1888)
/label=oYK215_Mcp_B_R
CDS 1843..1917
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
primer_bind complement(1843..1862)
/label=oYK92_L7Ae_R
primer_bind 1843..1857
/label=oYK82_LinkANR_F
primer_bind complement(1844..1863)
/label=test2
primer_bind 1869..1888
/label=oYK215_ANR1rep_F
primer_bind complement(1869..1888)
/label=oYK215_ANR1rep_R
primer_bind 1898..1917
/label=test1
primer_bind complement(1901..1917)
/label=TEST3
CDS 1918..1980
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
primer_bind 1918..1933
/label=Primer 4
primer_bind complement(1957..2000)
/label=oYK195_ANR_R
primer_bind complement(1962..1980)
/label=oYK82_LinkANR_R
primer_bind 1965..1979
/label=Primer 3
primer_bind complement(1976..2026)
/label=oYK215_Mcp_B_R
CDS 1981..2055
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
primer_bind complement(1981..2000)
/label=oYK92_L7Ae_R
primer_bind 1981..1995
/label=oYK82_LinkANR_F
primer_bind complement(1982..2001)
/label=test2
primer_bind 2007..2026
/label=oYK215_ANR1rep_F
primer_bind complement(2007..2026)
/label=oYK215_ANR1rep_R
primer_bind 2036..2055
/label=test1
primer_bind complement(2039..2055)
/label=TEST3
CDS 2056..2118
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
primer_bind 2056..2071
/label=Primer 4
primer_bind complement(2095..2118)
/label=oYK195_ANR_R
primer_bind complement(2100..2141)
/label=oYK82_LinkANR_R
primer_bind 2103..2117
/label=Primer 3
primer_bind complement(2119..2144)
/label=oYK472_NLS_R
primer_bind complement(2122..2144)
/label=oYK57-MCP-mCherry-R
terminator 2218..2445
/label=bGH PA
misc_feature 2491..2919
/label=f1 origin
misc_feature 2924..3267
/label=SV40 early promoter and origin
misc_feature 3113..3190
/label=SV40 ORI
CDS 3332..4123
/label=Kan/neoR
misc_feature 4297..4427
/label=SV40 early polyadenylation signal
primer_bind complement(4463..4483)
/label=M13-rev
misc_binding complement(4489..4511)
/label=LacO
promoter complement(4516..4545)
/label=lac
misc_feature complement(4810..5483)
/label=pUC origin
CDS complement(5631..6290)
/label=AmpR
promoter complement(6530..6558)
/label=Amp prom
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 atgtctaggc ctggagagag gcctttccag tgcaggatct gcatgaggaa cttttctgag
961 gaggccaacc tgaggaggca caccagaacc cacaccggag agaagccctt tcagtgcagg
1021 atttgcatga ggaatttctc cgaccacagc agcctgaaga gacacctgag aacccacaca
1081 ggaagccaga agcccttcca gtgcagaatc tgcatgagaa acttcagcca gagcgccaac
1141 ctgctgagac acaccagaac acacaccgga gaaaagccct tccagtgtag aatctgcatg
1201 cggaacttct ccgaccccag cagcctgaaa agacacctga ggacccacac aggcagccag
1261 aagccattcc agtgccgcat ctgcatgcgc aacttcagcc agcagaccaa cctgaccaga
1321 cacacccgca cccacaccgg cgaaaagcct ttccagtgtc gcatctgcat gagaaatttc
1381 agcgacgcca cccagctggt gagacacctg cggacacacc tgagaggcag cacctgcaga
1441 gactacaaag accatgacgg cgactataaa gatcacgaca tcgattacaa agacgacgac
1501 gacaagatgg ctccaaagaa gaagagaaag gtgggtgcca gcggaagtgg cggaggaggc
1561 gacgtcggct ccggaacggg cagcgggacc ggaagtggca ccggaaccac ttccggaacc
1621 ggtactggcg ggagtaccgg cggtgagcta gacgaactcg tgtacctgtt ggatggcccc
1681 ggttacgacc caatccacag cgatggctcc ggaacgggca gcgggaccgg aagtggcacc
1741 ggaaccactt ccggaaccgg tactggcggg agtaccggcg gtgagctaga cgaactcgtg
1801 tacctgttgg atggccccgg ttacgaccca atccacagcg atggctccgg aacgggcagc
1861 gggaccggaa gtggcaccgg aaccacttcc ggaaccggta ctggcgggag taccggcggt
1921 gagctagacg aactcgtgta cctgttggat ggccccggtt acgacccaat ccacagcgat
1981 ggctccggaa cgggcagcgg gaccggaagt ggcaccggaa ccacttccgg aaccggtact
2041 ggcgggagta ccggcggtga gctagacgaa ctcgtgtacc tgttggatgg ccccggttac
2101 gacccaatcc acagcgatta aggatccact agtccagtgt ggtggaattc tgcagatatc
2161 cagcacagtg gcggccgctc gagtctagag ggcccgttta aacccgctga tcagcctcga
2221 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
2281 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
2341 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
2401 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa
2461 gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg
2521 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
2581 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
2641 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
2701 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
2761 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
2821 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
2881 taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca
2941 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
3001 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
3061 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
3121 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
3181 tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt
3241 tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga
3301 tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc
3361 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg
3421 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac
3481 cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc
3541 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg
3601 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga
3661 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg
3721 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg
3781 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt
3841 cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc
3901 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg
3961 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga
4021 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc
4081 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc
4141 gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc
4201 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag
4261 cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat
4321 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
4381 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc
4441 tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
4501 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
4561 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
4621 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
4681 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
4741 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
4801 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
4861 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
4921 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
4981 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
5041 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
5101 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
5161 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
5221 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
5281 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
5341 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
5401 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
5461 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
5521 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
5581 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
5641 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
5701 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
5761 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
5821 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
5881 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
5941 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
6001 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
6061 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
6121 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
6181 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
6241 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
6301 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
6361 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
6421 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
6481 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
6541 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
6601 ccccgaaaag tgccacctga cgtc
//
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