| 质粒名称: | pYK195 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000799 |
| 产品用途: | Mammalian PhCMV-driven constitutive MCP-2xANR expression vector (PhCMV-MCP-2xANR-pA). |
| 存储实验室: | Anping ZENG Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pCMV |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CMV |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: |
Stbl3
|
|
插入基因信息 |
|
| 插入基因名称: | MCP-2xANR |
| 物种来源: | synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Programmable solid-state condensates for spatiotemporal control of mammalian gene expression
Wang Y., Jiang J., Xiong Q., Li S., Shao J., Xie M., Zeng A.P.
Nat. Chem. Biol. 2025 1–10 (2025)
|
pYK195
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pYK195载体质粒基本信息
pYK195质粒图谱载体图谱和pYK195载体序列质粒序列多克隆位点信息
pYK195质粒载体简介
pYK195质粒序列载体序列
LOCUS pYK195 6130 bp DNA circular SYN 11-FEB-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6130)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jul 27, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6130
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 232..819
/label=CMV
primer_bind 863..882
/label=T7
primer_bind 881..952
/label=oYK57-NLS-F
primer_bind 881..900
/label=oYK06-ANR-mEGFP-F
primer_bind 886..934
/label=oSCL1118
primer_bind 897..943
/label=oYK05-mcp-F
primer_bind 911..934
/label=Oscl32-1
primer_bind 911..934
/label=Oscl42-1
regulatory 918..926
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 926..1312
/codon_start=1
/product="bacteriophage MS2 coat protein"
/label=MS2-N55K
/note="binds with high affinity to a specific stem-loop
structure in the viral RNA (Lim et al., 1994)"
/translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
CSVRQSSAQKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
VKAMQGLLKDGNPIPSAIAANSGIY"
primer_bind 926..952
/label=oYK57-mcp-mCherry-F
primer_bind 926..952
/label=oYK228_mcp_F
primer_bind complement(1292..1342)
/label=oSCL093
primer_bind complement(1292..1312)
/label=Oscl32-2
primer_bind 1312..1332
/label=oLZ048 NSP3-F
misc_feature 1313..1347
/label=linker
primer_bind 1313..1342
/label=oSCL388
primer_bind 1313..1332
/label=oSCL1192
primer_bind 1313..1330
/label=oLZ147 NS3a F
primer_bind 1313..1329
/label=oYF0033
primer_bind 1313..1328
/label=oYK28-mCherry-NS3a-F
primer_bind complement(1323..1348)
/label=oSCL1119
primer_bind complement(1323..1342)
/label=oSCL075
primer_bind complement(1330..1348)
/label=oYK460_MCPGS_R
primer_bind complement(1331..1368)
/label=oYK82_mcp_R
primer_bind complement(1331..1349)
/label=oYK05-mcp-R
CDS 1349..1423
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
primer_bind complement(1349..1394)
/label=oYK215_Mcp_B_R
primer_bind complement(1349..1368)
/label=oYK195_ANR_R
primer_bind 1349..1363
/label=oYK82_LinkANR_F
primer_bind 1375..1394
/label=oYK215_ANR1rep_F
primer_bind complement(1375..1394)
/label=oYK215_ANR1rep_R
CDS 1424..1486
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
primer_bind 1424..1439
/label=Primer 4
primer_bind complement(1463..1506)
/label=oYK195_ANR_R
primer_bind complement(1468..1486)
/label=oYK82_LinkANR_R
primer_bind 1471..1485
/label=Primer 3
primer_bind complement(1482..1532)
/label=oYK215_Mcp_B_R
CDS 1487..1561
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
primer_bind complement(1487..1506)
/label=oYK82_mcp_R
primer_bind 1487..1501
/label=oYK82_LinkANR_F
primer_bind 1513..1532
/label=oYK215_ANR1rep_F
primer_bind complement(1513..1532)
/label=oYK215_ANR1rep_R
CDS 1562..1624
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
primer_bind 1562..1577
/label=Primer 4
primer_bind complement(1601..1624)
/label=oYK195_ANR_R
primer_bind complement(1606..1647)
/label=oYK82_LinkANR_R
primer_bind 1609..1623
/label=Primer 3
primer_bind complement(1628..1650)
/label=oYK57-MCP-mCherry-R
primer_bind complement(1628..1650)
/label=oYK409_BamXbaI_R
terminator 1724..1951
/label=bGH PA
misc_feature 1997..2425
/label=f1 origin
misc_feature 2430..2773
/label=SV40 early promoter and origin
misc_feature 2619..2696
/label=SV40 ORI
CDS 2838..3629
/label=Kan/neoR
misc_feature 3803..3933
/label=SV40 early polyadenylation signal
primer_bind complement(3969..3989)
/label=M13-rev
misc_binding complement(3995..4017)
/label=LacO
promoter complement(4022..4051)
/label=lac
misc_feature complement(4316..4989)
/label=pUC origin
CDS complement(5137..5796)
/label=AmpR
promoter complement(6036..6064)
/label=Amp prom
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 gtttaaactt aagcttgcca ccatggcttc aaactttact cagttcgtgc tcgtggacaa
961 tggtgggaca ggggatgtga cagtggctcc ttctaatttc gctaatgggg tggcagagtg
1021 gatcagctcc aactcacgga gccaggccta caaggtgaca tgcagcgtca ggcagtctag
1081 tgcccagaag agaaagtata ccatcaaggt ggaggtcccc aaagtggcta cccagacagt
1141 gggcggagtc gaactgcctg tcgccgcttg gaggtcctac ctgaacatgg agctcactat
1201 cccaattttc gctaccaatt ctgactgtga actcatcgtg aaggcaatgc aggggctcct
1261 caaagacggt aatcctatcc cttccgccat cgccgctaac tcaggtatct acgccagcgg
1321 aagtggcgga ggaggcgacg tcggatccgg ctccggaacg ggcagcggga ccggaagtgg
1381 caccggaacc acttccggaa ccggtactgg cgggagtacc ggcggtgagc tagacgaact
1441 cgtgtacctg ttggatggcc ccggttacga cccaatccac agcgatggct ccggaacggg
1501 cagcgggacc ggaagtggca ccggaaccac ttccggaacc ggtactggcg ggagtaccgg
1561 cggtgagcta gacgaactcg tgtacctgtt ggatggcccc ggttacgacc caatccacag
1621 cgattaagga tccactagtc cagtgtggtg gaattctgca gatatccagc acagtggcgg
1681 ccgctcgagt ctagagggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt
1741 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
1801 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
1861 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
1921 aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc
1981 tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
2041 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
2101 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
2161 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
2221 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
2281 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
2341 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
2401 atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa
2461 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
2521 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
2581 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
2641 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
2701 ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
2761 tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcaa gagacaggat
2821 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
2881 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg
2941 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg
3001 ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
3061 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
3121 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca
3181 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc
3241 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg
3301 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg
3361 cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata
3421 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg
3481 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat
3541 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
3601 tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca
3661 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt
3721 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
3781 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag
3841 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
3901 gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt
3961 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
4021 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
4081 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
4141 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
4201 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
4261 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
4321 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
4381 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
4441 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
4501 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
4561 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
4621 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
4681 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
4741 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
4801 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
4861 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
4921 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
4981 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
5041 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
5101 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
5161 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
5221 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
5281 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
5341 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
5401 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
5461 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
5521 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
5581 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
5641 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
5701 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
5761 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
5821 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
5881 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
5941 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
6001 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
6061 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
6121 acctgacgtc
//
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