pYK371

LOCUS      pYK371               7804 bp DNA     circular SYN 13-NOV-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 27, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7804
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    232..819
                     /label=CMV
     primer_bind     863..882
                     /label=T7
     primer_bind     880..984
                     /label=oCW60
     primer_bind     881..989
                     /label=oYK209_FUS_F
     primer_bind     881..928
                     /label=oYK449_NLS3_F
     primer_bind     881..900
                     /label=oYK06-ANR-mEGFP-F
     promoter        881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     887..904
                     /label=oYK661_Nhe_F
     primer_bind     902..921
                     /label=oYK809_NLS_F
     misc_feature    905..964
                     /label=NLS
     primer_bind     964..989
                     /label=oYK374_FUS_F
     primer_bind     964..984
                     /label=oCW41
     CDS             965..1609
                     /codon_start=1
                     /gene="FUS"
                     /gene_synonym="ALS6; altFUS; ETM4; FUS1; HNRNPP2; 
                     POMP75;TLS"
                     /product="RNA-binding protein FUS isoform 1"
                     /label=FUS
                     /note="isoform 1 is encoded by transcript variant 1; 
                     heterogeneous nuclear ribonucleoprotein P2; fused in 
                     sarcoma; fusion gene in myxoid liposarcoma; 75 kDa 
                     DNA-pairing protein; RNA-binding protein FUS; oncogene TLS;
                     fus-like protein; oncogene FUS; translocated in liposarcoma
                     protein"
                     /db_xref="CCDS:CCDS10707.1"
                     /db_xref="GeneID:2521"
                     /db_xref="HGNC:HGNC:4010"
                     /db_xref="MIM:137070"
                     /protein_id="NP_004951.1"
                     /translation="MASNDYTQQATQSYGAYPTQPGQGYSQQSSQPYGQQSYSGYSQST
                     DTSGYGQSSYSSYGQSQNTGYGTQSTPQGYGSTGGYGSSQSSQSSYGQQSSYPGYGQQP
                     APSSTSGSYGSSSQSSSYGQPQSGSYSQQPSYGGQQQSYGQQQSYNPPQGYGQQNQYNS
                     SSGGGGGGGGGGNYGQDQSSMSSGGGSGGGYGNQDQSGGGGSGGYGQQDRGG"
     primer_bind     965..991
                     /label=oYK397_FUS_F
     primer_bind     965..989
                     /label=oYK525_FUS_F
     primer_bind     968..993
                     /label=oYK339_FUS_F
     misc_feature    1040..1042
                     /gene="FUS"
                     /gene_synonym="ALS6; altFUS; ETM4; FUS1; HNRNPP2; 
                     POMP75;TLS"
                     /note="Phosphoserine./evidence=ECO:0000269|PubMed:29897835;
                      propagated from UniProtKB/Swiss-Prot (P35637.1); 
                     phosphorylation site"
     misc_feature    1052..1054
                     /gene="FUS"
                     /gene_synonym="ALS6; altFUS; ETM4; FUS1; HNRNPP2; 
                     POMP75;TLS"
                     /note="Phosphoserine./evidence=ECO:0000269|PubMed:29897835;
                      propagated from UniProtKB/Swiss-Prot (P35637.1); 
                     phosphorylation site"
     misc_feature    1088..1090
                     /gene="FUS"
                     /gene_synonym="ALS6; altFUS; ETM4; FUS1; HNRNPP2; 
                     POMP75;TLS"
                     /note="Phosphoserine, by ATM. 
                     /evidence=ECO:0000269|PubMed:18620545; propagated from 
                     UniProtKB/Swiss-Prot (P35637.1); phosphorylation site"
     misc_feature    1487..1489
                     /gene="FUS"
                     /gene_synonym="ALS6; altFUS; ETM4; FUS1; HNRNPP2; 
                     POMP75;TLS"
                     /note="Breakpoint for translocation to form 
                     chimericFUS/ATF1 protein; propagated from 
                     UniProtKB/Swiss-Prot (P35637.1); other site"
     primer_bind     complement(1579..1611)
                     /label=oCW42
     primer_bind     complement(1579..1611)
                     /label=oCW61
     primer_bind     1590..1631
                     /label=oYK68_FUS2_F
     primer_bind     1590..1609
                     /label=oYK69_AG_ANR1_F
     primer_bind     complement(1594..1609)
                     /label=oYK68_FUS1-R
     misc_feature    1610..2398
                     /label=FUS215-478
     primer_bind     1698..1712
                     /label=oYK68_FUS2_F
     primer_bind     complement(2377..2418)
                     /label=oYK88_FUS478_R
     misc_feature    2399..2626
                     /label=Long_Linker
     primer_bind     2399..2418
                     /label=oYK78_AG_ANR1_F
     primer_bind     2399..2416
                     /label=oYK69_AG_ANR1_F
     misc_feature    2627..3301
                     /label=AG
     primer_bind     3075..3085
                     /label=oSCL1104
     primer_bind     complement(3287..3324)
                     /label=oYK224_AG_R
     primer_bind     complement(3289..3301)
                     /label=oYK65-AG-R
     primer_bind     complement(3305..3327)
                     /label=oYK57-MCP-mCherry-R
     primer_bind     complement(3305..3324)
                     /label=oYK25-NS3a-R
     terminator      3401..3628
                     /label=bGH PA
     misc_feature    3674..4102
                     /label=f1 origin
     misc_feature    4107..4450
                     /label=SV40 early promoter and origin
     misc_feature    4296..4373
                     /label=SV40 ORI
     CDS             4515..5306
                     /label=Kan/neoR
     misc_feature    5480..5610
                     /label=SV40 early polyadenylation signal
     primer_bind     complement(5646..5666)
                     /label=M13-rev
     misc_binding    complement(5672..5694)
                     /label=LacO
     promoter        complement(5699..5728)
                     /label=lac
     primer_bind     5843..5854
                     /label=oYK69_AG_ANR1_F
     misc_feature    complement(5993..6663)
                     /label=pUC origin
     CDS             complement(6811..7470)
                     /label=AmpR
     promoter        complement(7710..7738)
                     /label=Amp prom
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 caccatgccc aagaagaagc gcaaggtgga agctagcgct tccccgaaga aaaagcggaa
      961 agtcatggcc tcaaacgatt atacccaaca agcaacccaa agctatgggg cctaccccac
     1021 ccagcccggg cagggctatt cccagcagag cagtcagccc tacggacagc agagttacag
     1081 tggttatagc cagtccacgg acacttcagg ctatggccag agcagctatt cttcttatgg
     1141 ccagagccag aacacaggct atggaactca gtcaactccc cagggatatg gctcgactgg
     1201 cggctatggc agtagccaga gctcccaatc gtcttacggg cagcagtcct cctaccctgg
     1261 ctatggccag cagccagctc ccagcagcac ctcgggaagt tacggtagca gttctcagag
     1321 cagcagctat gggcagcccc agagtgggag ctacagccag cagcctagct atggtggaca
     1381 gcagcaaagc tatggacagc agcaaagcta taatccccct cagggctatg gacagcagaa
     1441 ccagtacaac agcagcagtg gtggtggagg tggaggtgga ggtggaggta actatggcca
     1501 agatcaatcc tccatgagta gtggtggtgg cagtggtggc ggttatggca atcaagacca
     1561 gagtggtgga ggtggcagcg gtggctatgg acagcaggac cgtggaggca gaggcagagg
     1621 aggaagcgga ggaggaggag ggggaggagg aggcggatac aacagaagca gcggaggata
     1681 cgagccaaga ggaagaggag gaggaagagg agggagagga ggaatgggag gaagcgacag
     1741 aggaggattc aacaagttcg gaggaccaag agaccaggga agcagacacg acagcgagca
     1801 ggacaacagc gacaacaaca caatcttcgt gcagggactg ggcgagaatg tgaccatcga
     1861 gagcgtggcc gactacttca agcagatcgg aatcatcaag acaaacaaga aaacaggaca
     1921 gcccatgatc aacctgtaca ccgacagaga gactggcaag ctgaagggag aggccaccgt
     1981 gagcttcgac gaccccccca gcgccaaagc cgccatcgac tggttcgacg gcaaggagtt
     2041 cagcggcaac ccaatcaaag tgagctttgc caccagaaga gccgacttca acagaggcgg
     2101 aggcaacgga agaggcggaa gaggaagagg ggggccaatg ggcagaggag ggtatggcgg
     2161 aggaggaagc gggggaggag ggagaggggg attcccaagc ggaggagggg ggggaggagg
     2221 ccagcagaga gctggagact ggaagtgccc aaacccaaca tgcgagaaca tgaacttcag
     2281 ctggagaaac gagtgcaacc agtgcaaggc cccaaagcca gatggacccg gcggcggacc
     2341 aggaggaagc catatgggcg gcaactacgg cgacgacaga aggggcggca gaggcggagg
     2401 ttctgggagt ggcggtagtg gtgctggagg cagcgcaggc tctggagcag gcggcagtgc
     2461 cggaagtggc gctggagggt ctgccggatc tggagccggt ggttctgccg gctccggcgc
     2521 tggtgggagc gcttccggaa gtagtcccgg taattccgct gacggcggcg gaggatcggg
     2581 tggtagtggt ggttcaggag gaggatcgac ccaaggagga tccatggtga gcgtgatcaa
     2641 gcccgagatg aagatcaagc tgtgcatgag gggcaccgtg aacggccaca acttcgtgat
     2701 cgagggcgag ggcaagggca acccctacga gggcacccag atcctggacc tgaacgtgac
     2761 cgagggcgcc cccctgccct tcgcctacga catcctgacc accgtgttcc agtacggcaa
     2821 cagggccttc accaagtacc ccgccgacat ccaggactac ttcaagcaga ccttccccga
     2881 gggctaccac tgggagagga gcatgaccta cgaggaccag ggcatctgca ccgccaccag
     2941 caacatcagc atgaggggcg actgcttctt ctacgacatc aggttcgacg gcgtgaactt
     3001 cccccccaac ggccccgtga tgcagaagaa gaccctgaag tgggagccca gcaccgagaa
     3061 gatgtacgtg agggacggcg tgctgaaggg cgacgtgaac atggccctgc tgctggaggg
     3121 cggcggccac tacaggtgcg acttcaagac cacctacaag gccaagaagg acgtgaggct
     3181 gcccgactac cacttcgtgg accacaggat cgagatcctg aagcacgaca aggactacaa
     3241 caaggtgaag ctgtacgaga acgccgtggc caggtacagc atgctgccca gccaggccaa
     3301 gtaaggatcc actagtccag tgtggtggaa ttctgcagat atccagcaca gtggcggccg
     3361 ctcgagtcta gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc
     3421 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
     3481 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
     3541 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
     3601 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct
     3661 agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     3721 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     3781 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     3841 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     3901 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     3961 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     4021 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     4081 taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt
     4141 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     4201 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     4261 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     4321 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     4381 cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     4441 gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcaagag acaggatgag
     4501 gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg
     4561 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt
     4621 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc
     4681 tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt
     4741 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag
     4801 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg
     4861 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag
     4921 cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg
     4981 atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc
     5041 gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca
     5101 tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc
     5161 gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg
     5221 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct
     5281 atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc
     5341 gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg
     5401 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct
     5461 ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa
     5521 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     5581 caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc
     5641 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     5701 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     5761 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     5821 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     5881 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     5941 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     6001 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     6061 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     6121 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     6181 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     6241 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     6301 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     6361 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     6421 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     6481 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     6541 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtt tttttgtttg
     6601 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     6661 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     6721 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     6781 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     6841 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     6901 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     6961 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     7021 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     7081 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     7141 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     7201 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     7261 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     7321 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     7381 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     7441 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     7501 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     7561 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     7621 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     7681 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     7741 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     7801 cgtc
//