| 质粒名称: | pAAV-hSyn-GRAB_OTmut |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000154 |
| 产品用途: | Expresses the oxytocin-insensitive control sensor GRAB_OTmut in neurons |
| 存储实验室: | Yulong Li Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV |
| 表达系统: | 腺病毒，哺乳动物表达 |
| 启动子: | hSyn |
| 拷贝数: | 低拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a，TOP10 |
|
插入基因信息 |
|
| 插入基因名称: | GRAB_OTmut |
| 物种来源: | Synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
A genetically encoded sensor measures temporal oxytocin release from different neuronal compartments
Qian T., Wang H., Wang P., Geng L., Mei L., Osakada T., Tang Y., Kania A., Grinevich V., Stoop R., Lin D., Luo M., Li Y.
Nat. Biotechnol. 2023 417 41, 944–957 (2023)
|
买家导航
pAAV-hSyn-GRAB_OTmut载体质粒基本信息
pAAV-hSyn-GRAB_OTmut质粒图谱载体图谱和pAAV-hSyn-GRAB_OTmut载体序列质粒序列多克隆位点信息
pAAV-hSyn-GRAB_OTmut质粒载体简介
pAAV-hSyn-GRAB_OTmut质粒序列载体序列
LOCUS pAAV-hSyn-GRAB_OTmut 6410 bp DNA circular SYN 21-APR-2023
DEFINITION Expresses the genetically-encoded fluorescent oxytocin(OT) control
sensor GRAB_OTmut in neurons.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6410)
TITLE oxytocin sensors
REFERENCE 2 (bases 1 to 6410)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 28, 2026 from SnapGene 6.0.2
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..6410
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR (alternate)
/note="Functional equivalent of wild-type AAV2 ITR"
promoter 131..574
/label=hSyn
regulatory 581..590
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
sig_peptide 587..649
/codon_start=1
/product="leader sequence from mouse immunoglobulin kappa
light chain"
/label=Ig-kappa leader
/translation="METDTLLLWVLLLWVPGSTGD"
protein_bind 650..674
/gene="mutant version of attB"
/label=attB1
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
CDS 674..2512
/codon_start=1
/label=GRAB_OTmut
/translation="TTGEGAFAANWSAEAVNGSAAPPGTEGNRTAGPPQRNEALARVEV
AVLCLILFLALSGNACVLLALRTTRHKHSRLFFFMKHLSIADLVVAVFQVLPQLLWDIT
FRFYGPDLLCRLVKYLQVVGMFASTYLLLLMSLDRCLAICQPLRSLSRRTDRLAVLVTW
LGCLVASAPQVHIFSLREVADGVFDCWAVFIQPWGPKAYITWITLAVYIVPVIVLATCY
GLISFKIWQNLRLKTAGGNVYIKADKQKNGIKANFHIRHNIEDGGVQLAYHYQQNTPIG
DGPVLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVRK
GEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATEGKLTLKFICTTGKLPVPWPTLVT
TLTYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVN
RIELKGIDFKEDGNILGHKLEYNTGAAAKLISKAKIRTVKMTFIVVLAFIVCWTPFFFV
QMWSVWDADAPKEASPFIIAMLLASLNSCCNPWIYMLFTGHLFQELVQRFLCCSFRRLK
GSRPGETSVSKKSNSSTFVLSQYSSSQRRCSQPSTL"
primer_bind 1342..1363
/label=WKG153-F
primer_bind 1600..1621
/label=EGFP-C
/note="EGFP, forward primer"
primer_bind complement(1727..1748)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(1988..2007)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
misc_feature 2537..3125
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(2590..2610)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(3008..3019)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
polyA_signal 3157..3633
/label=hGH poly(A) signal
/note="human growth hormone polyadenylation signal"
primer_bind complement(3356..3375)
/label=hGH-PA-R
/note="Human growth hormone terminator, reverse primer"
repeat_region 3673..3813
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
repeat_region 3673..3802
/label=AAV2 ITR
rep_origin 3888..4343
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(3975..3994)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 4185..4206
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(4360..4379)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 4479..4501
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(4539..4557)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 4625..4729
/gene="bla"
/label=AmpR promoter
CDS 4730..5590
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(4948..4967)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 5761..6349
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 6250..6269
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtatgagg cggggtgggg gtgcctacct gacgaccgac
181 cccgacccac tggacaagca cccaaccccc attccccaaa ttgcgcatcc cctatcagag
241 agggggaggg gaaacaggat gcggcgaggc gcgtgcgcac tgccagcttc agcaccgcgg
301 acagtgcctt cgcccccgcc tggcggcgcg cgccaccgcc gcctcagcac tgaaggcgcg
361 ctgacgtcac tcgccggtcc cccgcaaact ccccttcccg gccaccttgg tcgcgtccgc
421 gccgccgccg gcccagccgg accgcaccac gcgaggcgcg agataggggg gcacgggcgc
481 gaccatctgc gctgcggcgc cggcgactca gcgctgcctc agtctgcggt gggcagcgga
541 ggagtcgtgt cgtgcctgag agcgcagtcg agaaaccggt gccaccatgg agacagacac
601 actcctgcta tgggtactgc tgctctgggt tccaggttcc actggtgaca caagtttgta
661 caaaaaagtt ggcaccaccg gtgagggtgc gtttgcggct aactggagcg ctgaggcggt
721 caacgggagc gcggcgccgc cgggaaccga gggcaatcgc actgccgggc cgccacagcg
781 caacgaggcc ctggcgcggg tggaggtggc cgtgctgtgc ctcatcctgt tcctggcgct
841 gagcggcaac gcgtgcgtgc tgctagcgct gcgcaccacg cgccacaagc actcgcgcct
901 cttcttcttc atgaagcacc tgagcatagc cgacctggta gtggcggtgt tccaggtgct
961 gccgcagctt ctgtgggaca tcacgttccg cttctacggg cccgacctgc tgtgccgcct
1021 cgtcaagtac ctgcaggttg tgggcatgtt cgcgtccacc tacctgctgc tgctcatgtc
1081 gctcgaccgc tgcctggcca tctgccagcc gctgcgctcg ctgagccgcc gcaccgaccg
1141 cctggcggta ctcgtcacat ggctcggctg cctggtggcc agcgcgccgc aggtgcacat
1201 cttctcgctg cgcgaggtgg ccgacggtgt cttcgactgc tgggccgttt tcattcaacc
1261 ctgggggccc aaggcctaca tcacgtggat cacgctcgcc gtctacattg tgcccgtcat
1321 cgtgcttgcc acctgctatg gccttatcag cttcaagatc tggcagaatt tacggctcaa
1381 gacggcggga ggaaacgtct atatcaaggc cgacaagcag aagaacggca tcaaggcgaa
1441 cttccacatc cgccacaaca tcgaggacgg cggcgtgcag ctcgcctacc actaccagca
1501 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcgtgca
1561 gtccaaactt tcgaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
1621 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagggcggta ccggagggag
1681 catggtgaga aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
1741 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggtgagggcg atgccaccga
1801 gggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
1861 cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa
1921 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac atccaggagc gcaccatctt
1981 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
2041 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
2101 caagctggag tacaacaccg gagcagcagc aaagctcatc tctaaggcca agatccgcac
2161 ggtcaagatg accttcatcg tcgtgctggc cttcatcgtg tgctggacgc cattcttttt
2221 cgtgcagatg tggagtgtct gggatgccga tgcgcccaag gaagcctcac ctttcatcat
2281 cgccatgctc ctggccagcc tcaacagctg ctgcaacccc tggatctaca tgctcttcac
2341 gggccacctc ttccaagaac ttgtgcagcg cttcctctgc tgctcattcc gccgcctgaa
2401 aggcagccgg cctggggaga caagcgtcag caaaaagagc aactcgtcta cctttgtcct
2461 gagccagtac agctccagcc agagaagatg ctcgcagcca tccacgctgt gagaattcga
2521 tatcaagctt atcgataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
2581 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
2641 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
2701 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
2761 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
2821 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
2881 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc
2941 ctttccttgg ctgctcgcct atgttgccac ctggattctg cgcgggacgt ccttctgcta
3001 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
3061 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
3121 cccgcatcga taccgagcgc tgctcgagag atctacgggt ggcatccctg tgacccctcc
3181 ccagtgcctc tcctggccct ggaagttgcc actccagtgc ccaccagcct tgtcctaata
3241 aaattaagtt gcatcatttt gtctgactag gtgtccttct ataatattat ggggtggagg
3301 ggggtggtat ggagcaaggg gcaagttggg aagacaacct gtagggcctg cggggtctat
3361 tgggaaccaa gctggagtgc agtggcacaa tcttggctca ctgcaatctc cgcctcctgg
3421 gttcaagcga ttctcctgcc tcagcctccc gagttgttgg gattccaggc atgcatgacc
3481 aggctcagct aatttttgtt tttttggtag agacggggtt tcaccatatt ggccaggctg
3541 gtctccaact cctaatctca ggtgatctac ccaccttggc ctcccaaatt gctgggatta
3601 caggcgtgaa ccactgctcc cttccctgtc cttctgattt tgtaggtaac cacgtgcgga
3661 ccgagcggcc gcaggaaccc ctagtgatgg agttggccac tccctctctg cgcgctcgct
3721 cgctcactga ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct
3781 cagtgagcga gcgagcgcgc agctgcctgc aggggcgcct gatgcggtat tttctcctta
3841 cgcatctgtg cggtatttca caccgcatac gtcaaagcaa ccatagtacg cgccctgtag
3901 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
3961 cgccttagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
4021 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
4081 cctcgacccc aaaaaacttg atttgggtga tggttcacgt agtgggccat cgccctgata
4141 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
4201 aactggaaca acactcaact ctatctcggg ctattctttt gatttataag ggattttgcc
4261 gatttcggtc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
4321 caaaatatta acgtttacaa ttttatggtg cactctcagt acaatctgct ctgatgccgc
4381 atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
4441 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
4501 gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt
4561 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
4621 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
4681 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
4741 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
4801 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
4861 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
4921 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
4981 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
5041 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
5101 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
5161 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
5221 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
5281 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
5341 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
5401 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
5461 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
5521 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
5581 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
5641 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
5701 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
5761 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
5821 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
5881 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
5941 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
6001 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
6061 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
6121 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
6181 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
6241 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
6301 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
6361 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
//
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