pDA077

LOCUS       pDA077               15364 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  L1RP expression.
ACCESSION   .
VERSION     .
KEYWORDS    pDA077
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15364)
  AUTHORS   Ardeljan D, Steranka JP, Liu C, Li Z, Taylor MS, Payer LM, Gorbounov
            M, Sarnecki JS, Deshpande V, Hruban RH, Boeke JD, Fenyo D, Wu PH, 
            Smogorzewska A, Holland AJ, Burns KH
  TITLE     Cell fitness screens reveal a conflict between LINE-1 
            retrotransposition and DNA replication.
  JOURNAL   Nat Struct Mol Biol. 2020 Feb;27(2):168-178. doi: 
            10.1038/s41594-020-0372-1. Epub 2020 Feb 10.
  PUBMED    32042151
REFERENCE   2  (bases 1 to 15364)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..15364
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     polyA_signal    6706..6840
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6730..6749)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6784..6803
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(6983..7005)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      7569..9358
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     primer_bind     complement(11398..11416)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        11484..11588
                     /gene="bla"
                     /label=AmpR promoter
     CDS             11589..12449
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11807..11826)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12620..13208
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13109..13128
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        13639..13784
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             13827..14621
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(13881..13900)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     14491..14510
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    14663..14711
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatctctag aagctgggta ccagctgcta gcaagcttgc tagcggccgc tctagccctg
      661 gaatgtgtat taagactgta aggtgggggg gaggagccaa gatggccgaa taggaacagc
      721 tccggtctac agctcccagc gtgagcgacg cagaagacgg tgatttctgc atttccatct
      781 gaggtaccgg gttcatctca ctagggagtg ccagacagtg ggcgcaggcc agtgtgtgtg
      841 cgcaccgtgc gcgagccgaa gcagggcgag gcattgcctc acctgggaag cgcaaggggt
      901 cagggagttc cctttccgag tcaaagaaag gggtgacgga cgcacctgga aaatcgggtc
      961 actcccaccc gaatattgcg cttttcagac cggcttaaga aacggcgcac cacgagacta
     1021 tatcccgcac ctggctcgga gggtcctacg cccacggaat ctcgctgatt gctagcacag
     1081 cagtctgaga tcaaactgca aggcggcaac gaggctgggg gaggggcgcc cgccattgcc
     1141 caggcttgct taggtaaaca aagcagcagg gaagctcgaa ctgggtggag cccaccacag
     1201 ctcaaggagg cctgcctgcc tctgtaggct ccacctctgg gggcagggca cagacaaaca
     1261 aaaagacagc agtaacctct gcagacttaa gtgtccctgt ctgacagctt tgaagagagc
     1321 agtggttctc ccagcacgca gctggagatc tgagaacggg cagactgcct cctcaagtgg
     1381 gtccctgacc cctgaccccc gagcagccta actgggaggc accccccagc aggggcacac
     1441 tgacacctca cacggcaggg tattccaaca gacctgcagc tgagggtcct gtctgttaga
     1501 aggaaaacta acaaccagaa aggacatcta caccgaaaac ccatctgtac atcaccatca
     1561 tcaaagacca aaagtagata aaaccacaaa gatggggaaa aaacagaaca gaaaaactgg
     1621 aaactctaaa acgcagagcg cctctcctcc tccaaaggaa cgcagttcct caccagcaac
     1681 agaacaaagc tggatggaga atgattttga tgagctgaga gaagaaggct tcagacgatc
     1741 aaattactct gagctacggg aggacattca aaccaaaggc aaagaagttg aaaactttga
     1801 aaaaaattta gaagaatgta taactagaat aaccaataca gagaagtgct taaaggagct
     1861 gatggagctg aaaaccaagg ctcgagaact acgtgaagaa tgcagaagcc tcaggagccg
     1921 atgcgatcaa ctggaagaaa gggtatcagc aatggaagat gaaatgaatg aaatgaagcg
     1981 agaagggaag tttagagaaa aaagaataaa aagaaatgag caaagcctcc aagaaatatg
     2041 ggactatgtg aaaagaccaa atctacgtct gattggtgta cctgaaagtg atgtggagaa
     2101 tggaaccaag ttggaaaaca ctctgcagga tattatccag gagaacttcc ccaatctagc
     2161 aaggcaggcc aacgttcaga ttcaggaaat acagagaacg ccacaaagat actcctcgag
     2221 aagagcaact ccaagacaca taattgtcag attcaccaaa gttgaaatga aggaaaaaat
     2281 gttaagggca gccagagaga aaggtcgggt taccctcaaa ggaaagccca tcagactaac
     2341 agcggatctc tcggcagaaa ccctacaagc cagaagagag tgggggccaa tattcaacat
     2401 tcttaaagaa aagaattttc aacccagaat ttcatatcca gccaaactaa gcttcataag
     2461 tgaaggagaa ataaaatact ttatagacaa gcaaatgttg agagattttg tcaccaccag
     2521 gcctgcccta aaagagctcc tgaaggaagc gctaaacatg gaaaggaaca accggtacca
     2581 gccgctgcaa aatcatgcca aaatgtaaag accatcaaga ctaggaagaa actgcaccaa
     2641 ctaatgagca aaatcaccag ctaacatcat aatgacagga tcaacttcac acataacaat
     2701 attaacttta aatataaatg gactaaattc tgcaattaaa agacacagac tggcaagttg
     2761 gataaagagt caagacccat cagtgtgctg tattcaggaa acccatctca cgtgcagaga
     2821 cacacatagg ctcaaaataa aaggatggag gaagatctac caagccaatg gaaaacaaaa
     2881 aaaggcaggg gttgcaatcc tagtctctga taaaacagac tttaaaccaa caaagatcaa
     2941 aagagacaaa gaaggccatt acataatggt aaagggatca attcaacaag aggagctaac
     3001 tatcctaaat atttatgcac ccaatacagg agcacccaga ttcataaagc aagtcctcag
     3061 tgacctacaa agagacttag actcccacac attaataatg ggagacttta acaccccact
     3121 gtcaacatta gacagatcaa cgagacagaa agtcaacaag gatacccagg aattgaactc
     3181 agctctgcac caagcagacc taatagacat ctacagaact ctccacccca aatcaacaga
     3241 atatacgttt ttttcagcac cacaccacac ctattccaaa attgaccaca tagttggaag
     3301 caaagctctc ctcagcaaat gtaaaagaac agaaattata acaaactatc tctcagacca
     3361 cagtgcaatc aaactagagc tcaggattaa gaatctcact caaagccgct caactacatg
     3421 gaaactgaac aacctgctcc tgaatgacta ctgggtacat aacgaaatga aggcagaaat
     3481 aaagatgttc tttgaaacca acgagaacaa agacaccaca taccagaatc tctgggacgc
     3541 attcaaagca gtatgtagag ggaaatttat agcactaaat gcctacaaga gaaagcagga
     3601 aagatccaaa attgacaccc taacatcaca attaaaagaa ctagaaaagc aagagcaaac
     3661 acattcaaaa gctagcagaa ggcaagaaat aactaaaatc agagcagaac tgaaggaaat
     3721 agagacacaa aaaacccttc aaaaaatcaa tgaatccagg agctggtttt ttgaaaggat
     3781 caacaaaatt gatagaccgc tagcaagact aataaagaaa aaaagagaga agaatcaaat
     3841 agacacaata aaaaatgata aaggggatat caccaccgat cccacagaaa tacaaactac
     3901 catcagagaa tactacaaac acctctacgc aaataaacta gaaaatctag aagaaatgga
     3961 tacattcctc gacacataca ctctcccaag actaaaccag gaagaagttg aatctctgaa
     4021 tcgaccaata acaggctctg aaattgtggc aataatcaat agtttaccaa ccaaaaagag
     4081 tccaggacca gatggattca cagccgaatt ctaccagagg tacaaggagg aactggtacc
     4141 attccttctg aaactattcc aatcaataga aaaagaggga atcctcccta actcatttta
     4201 tgaggccagc atcattctga taccaaagcc gggcagagac acaaccaaaa aagagaattt
     4261 tagaccaata tccttgatga acattgatgc aaaaatcctc aataaaatac tggcaaaccg
     4321 aatccagcag cacatcaaaa agcttatcca ccatgatcaa gtgggcttca tccctgggat
     4381 gcaaggctgg ttcaatatac gcaaatcaat aaatgtaatc cagcatataa acagagccaa
     4441 agacaaaaac cacatgatta tctcaataga tgcagaaaaa gcctttgaca aaattcaaca
     4501 acccttcatg ctaaaaactc tcaataaatt aggtattgat gggacgtatt tcaaaataat
     4561 aagagctatc tatgacaaac ccacagccaa tatcatactg aatgggcaaa aactggaagc
     4621 attccctttg aaaaccggca caagacaggg atgccctctc tcaccgctcc tattcaacat
     4681 agtgttggaa gttctggcca gggcaatcag gcaggagaag gaaataaagg gtattcaatt
     4741 aggaaaagag gaagtcaaat tgtccctgtt tgcagacgac atgattgttt atctagaaaa
     4801 ccccatcgtc tcagcccaaa atctccttaa gctgataagc aacttcagca aagtctcagg
     4861 atacaaaatc aatgtacaaa aatcacaagc attcttatac accaacaaca gacaaacaga
     4921 gagccaaatc atgggtgaac tcccattcac aattgcttca aagagaataa aatacctagg
     4981 aatccaactt acaagggatg tgaaggacct cttcaaggag aactacaaac cactgctcaa
     5041 ggaaataaaa gaggagacaa acaaatggaa gaacattcca tgctcatggg taggaagaat
     5101 caatatcgtg aaaatggcca tactgcccaa ggtaatttac agattcaatg ccatccccat
     5161 caagctacca atgactttct tcacagaatt ggaaaaaact actttaaagt tcatatggaa
     5221 ccaaaaaaga gcccgcattg ccaagtcaat cctaagccaa aagaacaaag ctggaggcat
     5281 cacactacct gacttcaaac tatactacaa ggctacagta accaaaacag catggtactg
     5341 gtaccaaaac agagatatag atcaatggaa cagaacagag ccctcagaaa taatgccgca
     5401 tatctacaac tatctgatct ttgacaaacc tgagaaaaac aagcaatggg gaaaggattc
     5461 cctatttaat aaatggtgct gggaaaactg gctagccata tgtagaaagc tgaaactgga
     5521 tcccttcctt acaccttata caaaaatcaa ttcaagatgg attaaagatt taaacgttaa
     5581 acctaaaacc ataaaaaccc tagaagaaaa cctaggcatt accattcagg acataggcgt
     5641 gggcaaggac ttcatgtcca aaacaccaaa agcaatggca acaaaagaca aaattgacaa
     5701 atgggatcta attaaactaa agagcttctg cacagcaaaa gaaactacca tcagagtgaa
     5761 caggcaacct acaacatggg agaaaatttt tgcaacctac tcatctgaca aagggctaat
     5821 atccagaatc tacaatgaac tcaaacaaat ttacaagaaa aaaacaaaca accccatcaa
     5881 aaagtgggcg aaggacatga acagacactt ctcaaaagaa gacatttatg cagccaaaaa
     5941 acacatgaag aaatgctcat catcactggc catcagagaa atgcaaatca aaaccactat
     6001 gagatatcat ctcacaccag ttagaatggc aatcattaaa aagtcaggaa acaacaggtg
     6061 ctggagagga tgcggagaaa taggaacact tttacactgt tggtgggact gtaaactagt
     6121 tcaaccattg tggaagtcag tgtggcgatt cctcagggat ctagaactag aaataccatt
     6181 tgacccagcc atcccattac tgggtatata cccaaatgag tataaatcat gctgctataa
     6241 agacacatgc acacgtatgt ttattgcggc actattcaca atagcaaaga cttggaacca
     6301 acccaaatgt ccaacaatga tagactggat taagaaaatg tggcacatat acaccatgga
     6361 atactatgca gccataaaaa atgatgagtt catatccttt gtagggacat ggatgaaatt
     6421 ggaaaccatc attctcagta aactatcgca agaacaaaaa accaaacacc gcatattctc
     6481 actcataggt gggaattgaa caatgagatc acatggacac aggaagggga atatcacact
     6541 ctggggactg tggtggggtc gggggagggg ggagggatag cattgggaga tatacctaat
     6601 gctagatgac acattagtgg gtgcagcgca ccagcatggc acatgtatac atatgtaact
     6661 aacctgcaca atgtgcacat gtaccctaaa acttagagta taatagatcc agacatgata
     6721 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
     6781 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
     6841 aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt
     6901 taaagcaagt aaaacctcta caaatgtggt atggctgatt atgatccggc tgcctcgcgc
     6961 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
     7021 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
     7081 ggtgtcgggg cgcagccatg aggtcgatcg actctagagg atcgatcccc gccccggacg
     7141 aactaaacct gactacgaca tctctgcccc ttcttcgcgg ggcagtgcat gtaatccctt
     7201 cagttggttg gtacaacttg ccaactgggc cctgttccac atgtgacacg gggggggacc
     7261 aaacacaaag gggttctctg actgtagttg acatccttat aaatggatgt gcacatttgc
     7321 caacactgag tggctttcat cctggagcag actttgcagt ctgtggactg caacacaaca
     7381 ttgcctttat gtgtaactct tggctgaagc tcttacacca atgctggggg acatgtacct
     7441 cccaggggcc caggaagact acgggaggct acaccaacgt caatcagagg ggcctgtgta
     7501 gctaccgata agcggaccct caagagggca ttagcaatag tgtttataag gcccccttgt
     7561 taaccctaaa cgggtagcat atgcttcccg ggtagtagta tatactatcc agactaaccc
     7621 taattcaata gcatatgtta cccaacggga agcatatgct atcgaattag ggttagtaaa
     7681 agggtcctaa ggaacagcga tatctcccac cccatgagct gtcacggttt tatttacatg
     7741 gggtcaggat tccacgaggg tagtgaacca ttttagtcac aagggcagtg gctgaagatc
     7801 aaggagcggg cagtgaactc tcctgaatct tcgcctgctt cttcattctc cttcgtttag
     7861 ctaatagaat aactgctgag ttgtgaacag taaggtgtat gtgaggtgct cgaaaacaag
     7921 gtttcaggtg acgcccccag aataaaattt ggacgggggg ttcagtggtg gcattgtgct
     7981 atgacaccaa tataaccctc acaaacccct tgggcaataa atactagtgt aggaatgaaa
     8041 cattctgaat atctttaaca atagaaatcc atggggtggg gacaagccgt aaagactgga
     8101 tgtccatctc acacgaattt atggctatgg gcaacacata atcctagtgc aatatgatac
     8161 tggggttatt aagatgtgtc ccaggcaggg accaagacag gtgaaccatg ttgttacact
     8221 ctatttgtaa caaggggaaa gagagtggac gccgacagca gcggactcca ctggttgtct
     8281 ctaacacccc cgaaaattaa acggggctcc acgccaatgg ggcccataaa caaagacaag
     8341 tggccactct tttttttgaa attgtggagt gggggcacgc gtcagccccc acacgccgcc
     8401 ctgcggtttt ggactgtaaa ataagggtgt aataacttgg ctgattgtaa ccccgctaac
     8461 cactgcggtc aaaccacttg cccacaaaac cactaatggc accccgggga atacctgcat
     8521 aagtaggtgg gcgggccaag ataggggcgc gattgctgcg atctggagga caaattacac
     8581 acacttgcgc ctgagcgcca agcacagggt tgttggtcct catattcacg aggtcgctga
     8641 gagcacggtg ggctaatgtt gccatgggta gcatatacta cccaaatatc tggatagcat
     8701 atgctatcct aatctatatc tgggtagcat aggctatcct aatctatatc tgggtagcat
     8761 atgctatcct aatctatatc tgggtagtat atgctatcct aatttatatc tgggtagcat
     8821 aggctatcct aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagtat
     8881 atgctatcct aatctgtatc cgggtagcat atgctatcct aatagagatt agggtagtat
     8941 atgctatcct aatttatatc tgggtagcat atactaccca aatatctgga tagcatatgc
     9001 tatcctaatc tatatctggg tagcatatgc tatcctaatc tatatctggg tagcataggc
     9061 tatcctaatc tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc
     9121 tatcctaatt tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc
     9181 tatcctaatc tatatctggg tagtatatgc tatcctaatc tgtatccggg tagcatatgc
     9241 tatcctcatg catatacagt cagcatatga tacccagtag tagagtggga gtgctatcct
     9301 ttgcatatgc cgccacctcc caagggggcg tgaattttcg ctgcttgtcc ttttcctgct
     9361 ggttgctccc attcttaggt gaatttaagg aggccaggct aaagccgtcg catgtctgat
     9421 tgctcaccag gtaaatgtcg ctaatgtttt ccaacgcgag aaggtgttga gcgcggagct
     9481 gagtgacgtg acaacatggg tatgcccaat tgccccatgt tgggaggacg aaaatggtga
     9541 caagacagat ggccagaaat acaccaacag cacgcatgat gtctactggg gatttattct
     9601 ttagtgcggg ggaatacacg gcttttaata cgattgaggg cgtctcctaa caagttacat
     9661 cactcctgcc cttcctcacc ctcatctcca tcacctcctt catctccgtc atctccgtca
     9721 tcaccctccg cggcagcccc ttccaccata ggtggaaacc agggaggcaa atctactcca
     9781 tcgtcaaagc tgcacacagt caccctgata ttgcaggtag gagcgggctt tgtcataaca
     9841 aggtccttaa tcgcatcctt caaaacctca gcaaatatat gagtttgtaa aaagaccatg
     9901 aaataacaga caatggactc ccttagcggg ccaggttgtg ggccgggtcc aggggccatt
     9961 ccaaagggga gacgactcaa tggtgtaaga cgacattgtg gaatagcaag ggcagttcct
    10021 cgccttaggt tgtaaaggga ggtcttacta cctccatata cgaacacacc ggcgacccaa
    10081 gttccttcgt cggtagtcct ttctacgtga ctcctagcca ggagagctct taaaccttct
    10141 gcaatgttct caaatttcgg gttggaacct ccttgaccac gatgctttcc aaaccaccct
    10201 ccttttttgc gcctgcctcc atcaccctga ccccggggtc cagtgcttgg gccttctcct
    10261 gggtcatctg cggggccctg ctctatcgct cccgggggca cgtcaggctc accatctggg
    10321 ccaccttctt ggtggtattc aaaataatcg gcttccccta cagggtggaa aaatggcctt
    10381 ctacctggag ggggcctgcg cggtggagac ccggatgatg atgactgact actgggactc
    10441 ctgggcctct tttctccacg tccacgacct ctccccctgg ctctttcacg acttcccccc
    10501 ctggctcttt cacgtcctct accccggcgg cctccactac ctcctcgacc ccggcctcca
    10561 ctacctcctc gaccccggcc tccactgcct cctcgacccc ggcctccacc tcctgctcct
    10621 gccnncctcc tgctcctgcc cctcctcctg ctcctgcccc tcctgctcct gcccctcccg
    10681 ctcctgctcc tgctcctgtt ccaccgtggg tccctttgca gccaatgcaa cttggacgtt
    10741 tttggggtct ccggacacca tctctatgtc ttggccctga tcctgagccg cccggggctc
    10801 ctggtcttcc gcctcctcgt cctcgtcctc ttccccgtcc tcgtccatgg ttatcacccc
    10861 ctcttctttg aggtccactg ccgccggagc cttctggtcc agatgtgtct cccttctctc
    10921 ctaggccatt tccaggtcct gtacctggcc cctcgtcaga catgattcac actaaaagag
    10981 atcaatagac atctttatta gacgacgctc agtgaataca gggagtgcag actcctgccc
    11041 cctccaacag cccccccacc ctcatcccct tcatggtcgc tgtcagacag atccaggtct
    11101 gaaaattccc catcctccga accatcctcg tcctcatcac caattactcg cagcccggaa
    11161 aactcccgct gaacatcctc aagatttgcg tcctgagcct caagccaggc ctcaaattcc
    11221 tcgtccccct ttttgctgga cggtagggat ggggattctc gggacccctc ctcttcctct
    11281 tcaaggtcac cagacagaga tgctactggg gcaacggaag aaaagctggg tgcggcctgt
    11341 gaggatcagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc
    11401 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
    11461 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
    11521 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
    11581 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
    11641 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
    11701 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
    11761 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
    11821 tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
    11881 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
    11941 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
    12001 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
    12061 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
    12121 ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
    12181 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
    12241 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
    12301 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
    12361 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
    12421 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
    12481 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
    12541 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
    12601 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
    12661 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
    12721 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
    12781 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
    12841 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
    12901 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
    12961 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
    13021 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
    13081 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
    13141 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
    13201 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttgaa
    13261 gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc
    13321 cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga
    13381 acgccagcaa gacgtagccc agcgcgtcgg ccccgagatg cgccgcgtgc ggctgctgga
    13441 gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg
    13501 caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccctgc
    13561 ttcatccccg tggcccgttg ctcgcgtttg ctggcggtgt ccccggaaga aatatatttg
    13621 catgtcttta gttctatgat gacacaaacc ccgcccagcg tcttgtcatt ggcgaattcg
    13681 aacacgcaga tgcagtcggg gcggcgcggt ccgaggtcca cttcgcatat taaggtgacg
    13741 cgtgtggcct cgaacaccga gcgaccctgc agcgacccgc ttaacagcgt caacagcgtg
    13801 ccgcagatcc cggggggcaa tgagatatga ttgaacaaga tggattgcac gcaggttctc
    13861 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct
    13921 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg
    13981 acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca
    14041 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc
    14101 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga
    14161 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc
    14221 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc
    14281 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg
    14341 ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct
    14401 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc
    14461 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc
    14521 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc
    14581 agcgcatcgc cttctatcgc cttcttgacg agttcttcta aaacacggaa ggagacaata
    14641 ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca cgggtgttgg
    14701 gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg cactctgtcg ataccccacc
    14761 gagaccccat tggggccaat acgcccgcgt ttcttccttt tccccacccc accccccaag
    14821 ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg ccatagccac
    14881 tggccccgtg ggttagggac ggggtacccc atggggaatg gtttatggtt cgtgggggtt
    14941 attattttgg gcgttgcgtg gggtcaggtc cacgactgga ctgagcagac agacccatgg
    15001 tttttggatg gcctgggcat ggaccgcatg tactggcgcg acacgaacac cgggcgtctg
    15061 tggctgccaa acacccccga cccccaaaaa ccaccgcgcg gatttctggc gtgccaagct
    15121 agtcgaccaa ttctcatgtt tgacagctta tcatcgcaga tccgggcaac gttgttgcca
    15181 ttgctgcagg cgcagaactg gtaggtatgg aagatctata cattgaatca atattggcaa
    15241 ttagccatat tagtcattgg ttatatagca taaatcaata ttggctattg gccattgcat
    15301 acgttgtatc tatatcataa tatgtacatt tatattggct catgtccaat atgaccgcca
    15361 tgtt
//