pCAMBIA1301-proMpEF1a-MpGLK-3xHA

LOCUS       pCAMBIA1301-proMpEF1a-MpGLK-3xHA             14851 bp DNA     circular SYN 22-FEB-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 26, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..14851
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1729
                     /label=proMpEF1a
     gene            1736..5705
                     /label=Mp7g09740
     CDS             5742..5831
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      5892..6144
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    6166..6190
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             7490..8119
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             8548..9621
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      9687..9881
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     misc_feature    10225..10365
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(10551..11139)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(11226..12020)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    12445..12469
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    12547..12721
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(12761..13786)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(13854..14531)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     protein_bind    14722..14743
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        14758..14788
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    14796..14812
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     14820..14836
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaatgagtc acacacattg ttgagagaca tatcaaaata actcaattgt aaggtaagtt
       61 gatagatttc ccagttaaat gcattatttt aacaatattt gtatctcaaa ttttcaaaat
      121 catatcaaat ttgcagatct tgatgatgat tcacaactct gtcttcgatt tttgattgtt
      181 aacgggtatc gatatttggg taacttaaaa agaatttgct aaagatctta aaaaacagat
      241 taaaaaaacc tttcaaaata gctaatcctt taaatccgtt tcacaatata atgctcattg
      301 atgataatcg aaaagattcc attttaaagt gcatataaat tgcttaatta tctctttctg
      361 tctcgtaata ttttgttctc tcctattgga tcggacccgc atcttctcga tcagttcact
      421 atactaatat ttggtcaact tcaagagaga ttagaacttc gttctccaaa gtgtaaaggc
      481 cgacaagcag aaaaggaaac ttcaatcaga agccttctag gttttcccgc gtcccaaaaa
      541 gcgaagggag gacggggagg acggtgaggc cagaagaaga aagcaacagt aaaatctcat
      601 gtgattgcgt gcatgtcatg gatctatggg atgctagtcc accgtttgga agtatgtgca
      661 gaaggagaaa ggcgcgggag gccaaaagcg acttcggcca cctctctact ttctagaaag
      721 tttgctcgca gcttgtaata agcgcggaaa aggtactcga aaaaccggag caatcggtgt
      781 ggtatcagca gtcgggttcg ctaaacctac gtcttcttat tttggaatgt taaattgaaa
      841 gggcgtgccg gccctaagca cggacgatgt gacagacgga ggagtgtgag caattcggat
      901 gacgatgctg gccggcagtc acgacaaaga gtccgaaggg gcatggacag ggcaatatat
      961 aaatggaaaa gaggctttga atattcctaa cccttcgtcg tcggcctccc aacttgccct
     1021 cgcatgctga attggccgcc tatctgcgaa tggccggccg ccgaccctaa gtcgcaataa
     1081 cttgaacgga gccgatgcaa agtccttatc gaacgctcgt tattataaac ggggcagacc
     1141 taggtattta attatatcaa aaccctccgc aatttcgcta atcattccct aatttcttct
     1201 actttggcgt ctagtgctgt ggtcgcgctt gctctcaaag ctcgctggga ggcagtggag
     1261 cgtctggctt aaaatcgatc gccgcaaccc tagagttcct ttcacttctt tctctcttgc
     1321 gtggagagca tcgacatcga agagctgcat cttgtcttct ggtatgcgcc ttgtgccctc
     1381 gtctctgtac gtcgttacga ttgctcgata ttgtgattgt ctagacctga aacccgactg
     1441 gagcttttac gtggtgtcga ttggggcctc gagcgagtga ttttttaggt tcgttttagc
     1501 caagtacatc ccactttggt cagtcctgtt atctcggtaa ggtgttcagt tcggtctctg
     1561 gtgcggaggt gcctcgtaag tttcctgcat gatagatttg tctatccttg acttgaagag
     1621 gagtcgaagg tcagcgaggt tggctgtgtg ctatggacgt cggtgctcct tggttccgtg
     1681 cttctatggg tgttgcctca acagtattga tgtgcctgca gaaaggttgg gtaccatgtt
     1741 tgcgttcaag gagaaattcc ccgactggaa ggatttccct aatggattgc gggttcttgt
     1801 tgtcgacgaa gacaccaaag tgctcaatga gattaagtgc aagttggaag gttgtcagta
     1861 tgtcggtgag tcctcctgtc tgattcatcg gatacagtga ccatggcctt gttcagcttg
     1921 tggcaagttg aggtcggtag ttcctaaagc gaaccaaaat tgggaggtgg agtagtcagt
     1981 ggtagaacgc gaaggttact gcagaaaaag cttgaccgga ggttaggatg agacggagtg
     2041 taggactcag agattctcat aggaacctca tttgcatggc aagggttcag gggatgcaaa
     2101 gaaattagta gaactatctg aagttaatcg aagatgtaca gttcctttct gatatgagat
     2161 gaaaggacga tgaactacag gccgttgcag gtttcagtcg tcatcgttat aagtgtatag
     2221 gtctctacgt tgcaaaattt gttcaatgga tcttttgaat agggccacta cctttcttat
     2281 cagttacgat cgtagggatc tcgttggtct gggcttgccc aaatgcatgc cagagaacaa
     2341 gcgcggagta ctgttatgga tgtgtactta cggactgtat atgctgaatg cacagtctcg
     2401 gctttcacta aaggagaaga tgcccttgaa gcattgcgag atcagcagaa catttttcac
     2461 gtggcgctgg tggaggtgaa aaccttgaca tgcaaatgcc atttgattct aggttgcaca
     2521 tttcatccaa tttactctcg ctttcaatcc attcagtttc caagtttttt ggaacctgcg
     2581 tcgtgattgt agtaatttga ctgcaggcaa tgacgggcga gggcttcaag atactggaag
     2641 ttgcaagaca tttgcctaca atatgtaagc agcacactca atctagtgcc gagtttactt
     2701 tgcattgaac gagatggtgt tttctacaag tcacgttcta acagcatcat attgcatgtc
     2761 gcagtgatgt ccaatacgga aaacatggcc atcatgatgc agggaattgc ggtacatctc
     2821 gattcatgtt ctgcgccgtt tcgttgaatt ttcagaactt tgaggtgata aacctgtgca
     2881 gtctgttttt aaagctgaag cttcggttat tctgatggtg gctctacgtg tcgcagcttg
     2941 gtgcagcgga ttttcttcaa aagccactgt ctgatgagaa gcttcggaac atttggcagc
     3001 atgttgttcg aaaggtaagc atcgtcactg ttgcaggaag gagcttttct taaatgtaaa
     3061 cctttcgaag cttgagtagt tggttgagta gtaaattttg gtgttattgc agttgtgagt
     3121 ccacagtttt gctaggattg tctggtgtac tttaagcttg ttcatatgac gttattttct
     3181 tatctgatga tagtactttc gagttttgca ggctctcaac acaggtgatc ctcttctgat
     3241 ggagtccctc gtacctgtta aagcagctgt ggaatccgtg ttttcgcttt ctccctcccc
     3301 aggtcgagtc aaagctgagc ctgtttcaag tccaggttct gatggggagt cgaagtcgaa
     3361 ggatatggag tgggttgctg gggtgacgaa atctccttca agcggagatg cagcagtttg
     3421 tgagagactt cctgcacctt caacaccaca gttggagcaa acagggagga tgaactcgca
     3481 agaagattca gtgtcagagt cacctgggtc tgatagcttt gtgcaagtga agaccgaggg
     3541 tgaggatccg gaaccagatg ctgccgcagg aaagccagca ccttgcacag aagtgaaagt
     3601 tgaggatcat gtggaggctg tttctgaatc ttctcaatta gacatcaagc ttgaagatta
     3661 tcctactgtc atcaagctag aattagatga taatatagat gatatcggtc tcagcaatgg
     3721 cattgtggta gatggtgatg gtgggtctgg attagacatc aatccttgcc tcctagtacc
     3781 tctccccgag tccgctctgg atatgcacat gaatattgag gctttggatg ccggagaagt
     3841 tcacgacact gagattgggg aagaggaggc ccttttgctt gcagaagtcg cgaaggctgg
     3901 agccgaaggc ctcgaacgaa ccccttctat gaattgcctt agcgttgaca gtggagattg
     3961 cagcagtgga gaaaagaagg gtgagggttg caaagtaaat aacaaggcta cgtcaggaag
     4021 aaggaaaatg aaggtacaga aagactgcaa catttttttt ggcttttgtt gaggttcatg
     4081 atcacatgaa cttactgtat gaggtgacgc aggggtctgt aataataata tgctaacccc
     4141 catcatttca ttctgttata aacaggttga ctggacccct gagctgcatc ggcggtttgt
     4201 ccaggcagtc gaacaactcg gagttgagaa ggctattcca tctcgcattt tagagctcat
     4261 gggagtacag tgtctaactc gtcacaatat tgccagccat ctgcaggtca gtgtgactcg
     4321 tgtcggtagt gtagaagtgc tgcctatttt tgacattgtc aaaatcgaca tcgtgacgtt
     4381 gcatcggtct gagtagcctc cggaatcccg gaaattttcg aaattgcgat atggttctga
     4441 gttttagctt tgggatcaat ggtttctcac cttggccaat catgcactca gaaatatcga
     4501 tctcatcgaa ggcatctttt ggcgagagaa gctgaagccg cgacttggca tcatcgcaaa
     4561 ccaattgatc caaatgtctg ggctaggagt agacgggatg gtactgcgtg gctggcccct
     4621 catcatacga accctccacc aattcagcct cgcccaccaa tgggtctcac gcctatacag
     4681 ccgcatccag gtgctcactg tcaccccatg ggaccgccga tgcatgtttg gggtcatcct
     4741 acgatggatc acaccgctgc gcacatgtgg cagcagccac aaatggccac accaacaaca
     4801 tggcaagcac ctgatggatc gtattggcag catccggtat gattcatccc cgtatattct
     4861 ggcgtccgaa cttgacgttc ttgacacatc cagcccaaag tgaacattgt gttatagtag
     4921 aattacgtcc ggaagatagc cacttccgat ttggggcatg ctctgacttt tttatttcaa
     4981 tttgtgcagt gtattgatgc ttggggtcat ccaacacctg gtccgggaac tccgtgctat
     5041 ccacagccat acgtacgttg ggctcatgac attaaccttt tgctggactg acgctccgag
     5101 gattatactg tgttgcgtgt gctgaaatta tggttattgg tgtaccgaga tagggctagc
     5161 taatgttaaa tatcggtcgt tatgttaatt gctatgatgc agagagttcc aatggctcct
     5221 atgccggcgt tcgcgtcacc catgacaact gctgcccttg cagccgacag ctatttcgct
     5281 gatgagtcga tgcctatacc catgtacccc actgcacccg atgacccgga attgactgtc
     5341 gcggctggcg ctgcggccag cagcaagcct tcagacttcc acccggtaag cttgatctga
     5401 ggggcagtcg gatcttactt tggttcagtt tttgcacgta tagatctgat ttgataaacg
     5461 tgaggcatct gtgattctga ccggtctctg tcgacgttcg ttggacaaag tgagactggg
     5521 tactgatttt ttacctgtct gtatgtgcgc agccaaagga gattctggac gccgcaatca
     5581 gtgaagcttt ggccaacccg tggacaccgt tgccacttgg tttgaagcct ccctctatgg
     5641 agggtgtgat ggctgagctc cagcggcagg gaatcaacac agttccacct cctcccctca
     5701 cttcgaagct ttcctctaga agcgggttaa ttaacatctt ttacccatac gatgttcctg
     5761 actatgcggg ctatccgtat gacgtcccgg actatgcagg atcctatcca tatgacgttc
     5821 cagattacgc tgctcagtgc agcgcttaga gctcgaattt ccccgatcgg gtgaccagct
     5881 cgaatttccc cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg
     5941 ccggtcttgc gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta
     6001 acatgtaatg catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat
     6061 acatttaata cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg
     6121 cggtgtcatc tatgttacta gatcgggaat taaactatca gtgtttgaca ggatatattg
     6181 gcgggtaaac ctaagagaaa agagcgttta ttagaataac ggatatttaa aagggcgtga
     6241 aaaggtttat ccgttcgtcc atttgtatgt gcatgccaac cacagggttc ccctcgggat
     6301 caaagtactt tgatccaacc cctccgctgc tatagtgcag tcggcttctg acgttcagtg
     6361 cagccgtctt ctgaaaacga catgtcgcac aagtcctaag ttacgcgaca ggctgccgcc
     6421 ctgccctttt cctggcgttt tcttgtcgcg tgttttagtc gcataaagta gaatacttgc
     6481 gactagaacc ggagacatta cgccatgaac aagagcgccg ccgctggcct gctgggctat
     6541 gcccgcgtca gcaccgacga ccaggacttg accaaccaac gggccgaact gcacgcggcc
     6601 ggctgcacca agctgttttc cgagaagatc accggcacca ggcgcgaccg cccggagctg
     6661 gccaggatgc ttgaccacct acgccctggc gacgttgtga cagtgaccag gctagaccgc
     6721 ctggcccgca gcacccgcga cctactggac attgccgagc gcatccagga ggccggcgcg
     6781 ggcctgcgta gcctggcaga gccgtgggcc gacaccacca cgccggccgg ccgcatggtg
     6841 ttgaccgtgt tcgccggcat tgccgagttc gagcgttccc taatcatcga ccgcacccgg
     6901 agcgggcgcg aggccgccaa ggcccgaggc gtgaagtttg gcccccgccc taccctcacc
     6961 ccggcacaga tcgcgcacgc ccgcgagctg atcgaccagg aaggccgcac cgtgaaagag
     7021 gcggctgcac tgcttggcgt gcatcgctcg accctgtacc gcgcacttga gcgcagcgag
     7081 gaagtgacgc ccaccgaggc caggcggcgc ggtgccttcc gtgaggacgc attgaccgag
     7141 gccgacgccc tggcggccgc cgagaatgaa cgccaagagg aacaagcatg aaaccgcacc
     7201 aggacggcca ggacgaaccg tttttcatta ccgaagagat cgaggcggag atgatcgcgg
     7261 ccgggtacgt gttcgagccg cccgcgcacg tctcaaccgt gcggctgcat gaaatcctgg
     7321 ccggtttgtc tgatgccaag ctggcggcct ggccggccag cttggccgct gaagaaaccg
     7381 agcgccgccg tctaaaaagg tgatgtgtat ttgagtaaaa cagcttgcgt catgcggtcg
     7441 ctgcgtatat gatgcgatga gtaaataaac aaatacgcaa ggggaacgca tgaaggttat
     7501 cgctgtactt aaccagaaag gcgggtcagg caagacgacc atcgcaaccc atctagcccg
     7561 cgccctgcaa ctcgccgggg ccgatgttct gttagtcgat tccgatcccc agggcagtgc
     7621 ccgcgattgg gcggccgtgc gggaagatca accgctaacc gttgtcggca tcgaccgccc
     7681 gacgattgac cgcgacgtga aggccatcgg ccggcgcgac ttcgtagtga tcgacggagc
     7741 gccccaggcg gcggacttgg ctgtgtccgc gatcaaggca gccgacttcg tgctgattcc
     7801 ggtgcagcca agcccttacg acatatgggc caccgccgac ctggtggagc tggttaagca
     7861 gcgcattgag gtcacggatg gaaggctaca agcggccttt gtcgtgtcgc gggcgatcaa
     7921 aggcacgcgc atcggcggtg aggttgccga ggcgctggcc gggtacgagc tgcccattct
     7981 tgagtcccgt atcacgcagc gcgtgagcta cccaggcact gccgccgccg gcacaaccgt
     8041 tcttgaatca gaacccgagg gcgacgctgc ccgcgaggtc caggcgctgg ccgctgaaat
     8101 taaatcaaaa ctcatttgag ttaatgaggt aaagagaaaa tgagcaaaag cacaaacacg
     8161 ctaagtgccg gccgtccgag cgcacgcagc agcaaggctg caacgttggc cagcctggca
     8221 gacacgccag ccatgaagcg ggtcaacttt cagttgccgg cggaggatca caccaagctg
     8281 aagatgtacg cggtacgcca aggcaagacc attaccgagc tgctatctga atacatcgcg
     8341 cagctaccag agtaaatgag caaatgaata aatgagtaga tgaattttag cggctaaagg
     8401 aggcggcatg gaaaatcaag aacaaccagg caccgacgcc gtggaatgcc ccatgtgtgg
     8461 aggaacgggc ggttggccag gcgtaagcgg ctgggttgtc tgccggccct gcaatggcac
     8521 tggaaccccc aagcccgagg aatcggcgtg agcggtcgca aaccatccgg cccggtacaa
     8581 atcggcgcgg cgctgggtga tgacctggtg gagaagttga aggccgcgca ggccgcccag
     8641 cggcaacgca tcgaggcaga agcacgcccc ggtgaatcgt ggcaagcggc cgctgatcga
     8701 atccgcaaag aatcccggca accgccggca gccggtgcgc cgtcgattag gaagccgccc
     8761 aagggcgacg agcaaccaga ttttttcgtt ccgatgctct atgacgtggg cacccgcgat
     8821 agtcgcagca tcatggacgt ggccgttttc cgtctgtcga agcgtgaccg acgagctggc
     8881 gaggtgatcc gctacgagct tccagacggg cacgtagagg tttccgcagg gccggccggc
     8941 atggccagtg tgtgggatta cgacctggta ctgatggcgg tttcccatct aaccgaatcc
     9001 atgaaccgat accgggaagg gaagggagac aagcccggcc gcgtgttccg tccacacgtt
     9061 gcggacgtac tcaagttctg ccggcgagcc gatggcggaa agcagaaaga cgacctggta
     9121 gaaacctgca ttcggttaaa caccacgcac gttgccatgc agcgtacgaa gaaggccaag
     9181 aacggccgcc tggtgacggt atccgagggt gaagccttga ttagccgcta caagatcgta
     9241 aagagcgaaa ccgggcggcc ggagtacatc gagatcgagc tagctgattg gatgtaccgc
     9301 gagatcacag aaggcaagaa cccggacgtg ctgacggttc accccgatta ctttttgatc
     9361 gatcccggca tcggccgttt tctctaccgc ctggcacgcc gcgccgcagg caaggcagaa
     9421 gccagatggt tgttcaagac gatctacgaa cgcagtggca gcgccggaga gttcaagaag
     9481 ttctgtttca ccgtgcgcaa gctgatcggg tcaaatgacc tgccggagta cgatttgaag
     9541 gaggaggcgg ggcaggctgg cccgatccta gtcatgcgct accgcaacct gatcgagggc
     9601 gaagcatccg ccggttccta atgtacggag cagatgctag ggcaaattgc cctagcaggg
     9661 gaaaaaggtc gaaaaggtct ctttcctgtg gatagcacgt acattgggaa cccaaagccg
     9721 tacattggga accggaaccc gtacattggg aacccaaagc cgtacattgg gaaccggtca
     9781 cacatgtaag tgactgatat aaaagagaaa aaaggcgatt tttccgccta aaactcttta
     9841 aaacttatta aaactcttaa aacccgcctg gcctgtgcat aactgtctgg ccagcgcaca
     9901 gccgaagagc tgcaaaaagc gcctaccctt cggtcgctgc gctccctacg ccccgccgct
     9961 tcgcgtcggc ctatcgcggc cgctggccgc tcaaaaatgg ctggcctacg gccaggcaat
    10021 ctaccagggc gcggacaagc cgcgccgtcg ccactcgacc gccggcgccc acatcaaggc
    10081 accctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
    10141 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
    10201 agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt
    10261 gtatactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg
    10321 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc
    10381 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
    10441 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
    10501 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
    10561 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
    10621 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
    10681 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
    10741 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
    10801 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
    10861 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
    10921 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
    10981 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
    11041 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
    11101 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
    11161 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgcattct
    11221 aggtactaaa acaattcatc cagtaaaata taatatttta ttttctccca atcaggcttg
    11281 atccccagta agtcaaaaaa tagctcgaca tactgttctt ccccgatatc ctccctgatc
    11341 gaccggacgc agaaggcaat gtcataccac ttgtccgccc tgccgcttct cccaagatca
    11401 ataaagccac ttactttgcc atctttcaca aagatgttgc tgtctcccag gtcgccgtgg
    11461 gaaaagacaa gttcctcttc gggcttttcc gtctttaaaa aatcatacag ctcgcgcgga
    11521 tctttaaatg gagtgtcttc ttcccagttt tcgcaatcca catcggccag atcgttattc
    11581 agtaagtaat ccaattcggc taagcggctg tctaagctat tcgtataggg acaatccgat
    11641 atgtcgatgg agtgaaagag cctgatgcac tccgcataca gctcgataat cttttcaggg
    11701 ctttgttcat cttcatactc ttccgagcaa aggacgccat cggcctcact catgagcaga
    11761 ttgctccagc catcatgccg ttcaaagtgc aggacctttg gaacaggcag ctttccttcc
    11821 agccatagca tcatgtcctt ttcccgttcc acatcatagg tggtcccttt ataccggctg
    11881 tccgtcattt ttaaatatag gttttcattt tctcccacca gcttatatac cttagcagga
    11941 gacattcctt ccgtatcttt tacgcagcgg tatttttcga tcagtttttt caattccggt
    12001 gatattctca ttttagccat ttattatttc cttcctcttt tctacagtat ttaaagatac
    12061 cccaagaagc taattataac aagacgaact ccaattcact gttccttgca ttctaaaacc
    12121 ttaaatacca gaaaacagct ttttcaaagt tgttttcaaa gttggcgtat aacatagtat
    12181 cgacggagcc gattttgaaa ccgcggtgat cacaggcagc aacgctctgt catcgttaca
    12241 atcaacatgc taccctccgc gagatcatcc gtgtttcaaa cccggcagct tagttgccgt
    12301 tcttccgaat agcatcggta acatgagcaa agtctgccgc cttacaacgg ctctcccgct
    12361 gacgccgtcc cggactgatg ggctgcctgt atcgagtggt gattttgtgc cgagctgccg
    12421 gtcggggagc tgttggctgg ctggtggcag gatatattgt ggtgtaaaca aattgacgct
    12481 tagacaactt aataacacat tgcggacgtt tttaatgtac tgaattaacg ccgaattaat
    12541 tcgggggatc tggattttag tactggattt tggttttagg aattagaaat tttattgata
    12601 gaagtatttt acaaatacaa atacatacta agggtttctt atatgctcaa cacatgagcg
    12661 aaaccctata ggaaccctaa ttcccttatc tgggaactac tcacacatta ttatggagaa
    12721 actcgagctt gtcgatcgac agatccggtc ggcatctact ctatttcttt gccctcggac
    12781 gagtgctggg gcgtcggttt ccactatcgg cgagtacttc tacacagcca tcggtccaga
    12841 cggccgcgct tctgcgggcg atttgtgtac gcccgacagt cccggctccg gatcggacga
    12901 ttgcgtcgca tcgaccctgc gcccaagctg catcatcgaa attgccgtca accaagctct
    12961 gatagagttg gtcaagacca atgcggagca tatacgcccg gagtcgtggc gatcctgcaa
    13021 gctccggatg cctccgctcg aagtagcgcg tctgctgctc catacaagcc aaccacggcc
    13081 tccagaagaa gatgttggcg acctcgtatt gggaatcccc gaacatcgcc tcgctccagt
    13141 caatgaccgc tgttatgcgg ccattgtccg tcaggacatt gttggagccg aaatccgcgt
    13201 gcacgaggtg ccggacttcg gggcagtcct cggcccaaag catcagctca tcgagagcct
    13261 gcgcgacgga cgcactgacg gtgtcgtcca tcacagtttg ccagtgatac acatggggat
    13321 cagcaatcgc gcatatgaaa tcacgccatg tagtgtattg accgattcct tgcggtccga
    13381 atgggccgaa cccgctcgtc tggctaagat cggccgcagc gatcgcatcc atagcctccg
    13441 cgaccggttg tagaacagcg ggcagttcgg tttcaggcag gtcttgcaac gtgacaccct
    13501 gtgcacggcg ggagatgcaa taggtcaggc tctcgctaaa ctccccaatg tcaagcactt
    13561 ccggaatcgg gagcgcggcc gatgcaaagt gccgataaac ataacgatct ttgtagaaac
    13621 catcggcgca gctatttacc cgcaggacat atccacgccc tcctacatcg aagctgaaag
    13681 cacgagattc ttcgccctcc gagagctgca tcaggtcgga gacgctgtcg aacttttcga
    13741 tcagaaactt ctcgacagac gtcgcggtga gttcaggctt tttcatatct cattgccccc
    13801 cgggatctgc gaaagctcga gagagataga tttgtagaga gagactggtg atttcagcgt
    13861 gtcctctcca aatgaaatga acttccttat atagaggaag gtcttgcgaa ggatagtggg
    13921 attgtgcgtc atcccttacg tcagtggaga tatcacatca atccacttgc tttgaagacg
    13981 tggttggaac gtcttctttt tccacgatgc tcctcgtggg tgggggtcca tctttgggac
    14041 cactgtcggc agaggcatct tgaacgatag cctttccttt atcgcaatga tggcatttgt
    14101 aggtgccacc ttccttttct actgtccttt tgatgaagtg acagatagct gggcaatgga
    14161 atccgaggag gtttcccgat attacccttt gttgaaaagt ctcaatagcc ctttggtctt
    14221 ctgagactgt atctttgata ttcttggagt agacgagagt gtcgtgctcc accatgttat
    14281 cacatcaatc cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc
    14341 tcgtgggtgg gggtccatct ttgggaccac tgtcggcaga ggcatcttga acgatagcct
    14401 ttcctttatc gcaatgatgg catttgtagg tgccaccttc cttttctact gtccttttga
    14461 tgaagtgaca gatagctggg caatggaatc cgaggaggtt tcccgatatt accctttgtt
    14521 gaaaagtctc aatagccctt tggtcttctg agactgtatc tttgatattc ttggagtaga
    14581 cgagagtgtc gtgctccacc atgttggcaa gctgctctag ccaatacgca aaccgcctct
    14641 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc
    14701 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
    14761 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
    14821 aggaaacagc tatgaccatg attacgaatt c
//