| 质粒名称: | MS2-P65-HSF1_GFP |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 61423 |
| 存储实验室: | Feng Zhang |
|
载体骨架基本信息 |
|
| 载体名称: | lenti(AMP) |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | MS2(N55K)-P65-HSF1_2A_GFP |
| 物种来源: | --- |
| 插入大小： | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | AgeI(未破坏) |
| 3' 克隆位点: | NotI (未破坏) |
| 5' 测序引物 | CMV-F |
| 3' 测序引物 | SV40pA-R |
|
文献引用方法 |
|
| 材料和方法部分: | MS2-P65-HSF1_GFP was a gift from Feng Zhang (Addgene plasmid # 61423 ; http://n2t.net/addgene:61423 ; RRID:Addgene_61423) |
| 参考文献部分: | Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex. Konermann S, Brigham MD, Trevino AE, Joung J, Abudayyeh OO, Barcena C, Hsu PD, Habib N, Gootenberg JS, Nishimasu H, Nureki O, Zhang F. Nature. 2014 Dec 10. doi: 10.1038/nature14136. 10.1038/nature14136 PubMed 25494202 |
买家导航
MS2-P65-HSF1_GFP载体质粒基本信息
MS2-P65-HSF1_GFP质粒图谱载体图谱和MS2-P65-HSF1_GFP载体序列质粒序列多克隆位点信息
MS2-P65-HSF1_GFP质粒载体简介
MS2-P65-HSF1_GFP质粒序列载体序列
LOCUS MS2-P65-HSF1_GFP 11409 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Expresses the MS2-P65-HSF1 activator helper complex with a 2A GFP.
3rd generation lentiviral vector..
ACCESSION .
VERSION .
KEYWORDS MS2-P65-HSF1_GFP
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11409)
AUTHORS Konermann S, Brigham MD, Trevino AE, Joung J, Abudayyeh OO, Barcena
C, Hsu PD, Habib N, Gootenberg JS, Nishimasu H, Nureki O, Zhang F
TITLE Genome-scale transcriptional activation by an engineered CRISPR-Cas9
complex.
JOURNAL Nature. 2014 Dec 10. doi: 10.1038/nature14136.
PUBMED 25494202
REFERENCE 2 (bases 1 to 11409)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..11409
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(47..66)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 238..617
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 619..817
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 772..792
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
LTR 835..1015
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 1062..1187
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1680..1913
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
misc_feature 2440..2557
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 2682..3859
/label=EF-1-alpha promoter
/note="strong constitutive promoter for human elongation
factor EF-1-alpha"
intron 2913..3850
/label=EF-1-alpha intron A
/note="intron upstream of the start codon of human
EF-1-alpha"
primer_bind 3807..3827
/label=EF1a-F
/note="Human elongation factor-1a promoter, forward primer"
regulatory 3866..3875
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 3875..4261
/codon_start=1
/product="bacteriophage MS2 coat protein"
/label=MS2-N55K
/note="binds with high affinity to a specific stem-loop
structure in the viral RNA (Lim et al., 1994)"
/translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
CSVRQSSAQKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
VKAMQGLLKDGNPIPSAIAANSGIY"
CDS 4316..4336
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 4352..4894
/codon_start=1
/product="C-terminal portion of the p65 subunit of mouse
NF-kappa-B"
/label=p65
/translation="PSGQISNQALALAPSSAPVLAQTMVPSSAMVPLAQPPAPAPVLTP
GPPQSLSAPVPKSTQAGEGTLSEALLHLQFDADEDLGALLGNSTDPGVFTDLASVDNSE
FQQLLNQGVSMSHSTAEPMLMEYPEAITRLVTGSQRPPDPAPTPLGTSGLPNGLSGDED
FSSIADMDFSALLSQISS"
CDS 4919..5290
/codon_start=1
/product="C-terminal activation domain from the human heat
shock transcription factor HSF1"
/label=HSF1
/translation="GFSVDTSALLDLFSPSVTVPDMSLPDLDSSLASIQELLSPQEPPR
PPEAENSSPDSGKQLVHYTAQPLFLLDPGSVDTGSNDLPVLFELGEGSYFSEGDGFAED
PTISLLTGSEPPKAKDPTVS"
CDS 5306..5359
/codon_start=1
/product="2A peptide from Thosea asigna virus capsid
protein"
/label=T2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="EGRGSLLTCGDVEENPGP"
CDS 5360..6076
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/note="mammalian codon-optimized"
/translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
FVTAAGITLGMDELYK"
primer_bind complement(5402..5423)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(5663..5682)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 6010..6031
/label=EGFP-C
/note="EGFP, forward primer"
misc_feature 6101..6689
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(6154..6174)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(6572..6583)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
primer_bind complement(6692..6708)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(6693..6709)
/label=pBluescriptKS
/note="For pBluescript vector"
LTR 7214..7394
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
primer_bind complement(7420..7437)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 7426..7650
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 7696..8124
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(7783..7802)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 7993..8014
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(8133..8153)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 8138..8467
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 8318..8453
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 8380..8399
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
promoter 8515..8562
/label=EM7 promoter
/note="synthetic bacterial promoter "
CDS 8581..8955
/codon_start=1
/gene="Sh ble from Streptoalloteichus hindustanus"
/product="antibiotic-binding protein"
/label=BleoR
/note="confers resistance to bleomycin, phleomycin, and
Zeocin(TM)"
/translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
EFALRDPAGNCVHFVAEEQD"
polyA_signal 9085..9206
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(9122..9141)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 9176..9195
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(9255..9271)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(9255..9271)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(9268..9290)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 9279..9295
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(9303..9333)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 9348..9369
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(9486..9503)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(9657..10245)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(9737..9756)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(10416..11276)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 11039..11058
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(11277..11381)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
2581 agatccagtt tggttaatta atgcaaagat ggataaagtt ttaaacagag aggaatcttt
2641 gcagctaatg gaccttctag gtcttgaaag gagtgggaat tggctccggt gcccgtcagt
2701 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
2761 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
2821 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
2881 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc
2941 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccact ggctgcagta
3001 cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga ggccttgcgc
3061 ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct ggggccgccg
3121 cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt ctctagccat
3181 ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc ttgtaaatgc
3241 gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg acggggcccg
3301 tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac cgagaatcgg
3361 acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc cgccgtgtat
3421 cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag cggaaagatg
3481 gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct cgggagagcg
3541 ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg tcgcttcatg
3601 tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga gcttttggag
3661 tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc acactgagtg
3721 ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg aatttgccct
3781 ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa gtttttttct
3841 tccatttcag gtgtcgtgac gtacggccac catggcttca aactttactc agttcgtgct
3901 cgtggacaat ggtgggacag gggatgtgac agtggctcct tctaatttcg ctaatggggt
3961 ggcagagtgg atcagctcca actcacggag ccaggcctac aaggtgacat gcagcgtcag
4021 gcagtctagt gcccagaaga gaaagtatac catcaaggtg gaggtcccca aagtggctac
4081 ccagacagtg ggcggagtcg aactgcctgt cgccgcttgg aggtcctacc tgaacatgga
4141 gctcactatc ccaattttcg ctaccaattc tgactgtgaa ctcatcgtga aggcaatgca
4201 ggggctcctc aaagacggta atcctatccc ttccgccatc gccgctaact caggtatcta
4261 cagcgctgga ggaggtggaa gcggaggagg aggaagcgga ggaggaggta gcggacctaa
4321 gaaaaagagg aaggtggcgg ccgctggatc cccttcaggg cagatcagca accaggccct
4381 ggctctggcc cctagctccg ctccagtgct ggcccagact atggtgccct ctagtgctat
4441 ggtgcctctg gcccagccac ctgctccagc ccctgtgctg accccaggac caccccagtc
4501 actgagcgct ccagtgccca agtctacaca ggccggcgag gggactctga gtgaagctct
4561 gctgcacctg cagttcgacg ctgatgagga cctgggagct ctgctgggga acagcaccga
4621 tcccggagtg ttcacagatc tggcctccgt ggacaactct gagtttcagc agctgctgaa
4681 tcagggcgtg tccatgtctc atagtacagc cgaaccaatg ctgatggagt accccgaagc
4741 cattacccgg ctggtgaccg gcagccagcg gccccccgac cccgctccaa ctcccctggg
4801 aaccagcggc ctgcctaatg ggctgtccgg agatgaagac ttctcaagca tcgctgatat
4861 ggactttagt gccctgctgt cacagatttc ctctagtggg cagggaggag gtggaagcgg
4921 cttcagcgtg gacaccagtg ccctgctgga cctgttcagc ccctcggtga ccgtgcccga
4981 catgagcctg cctgaccttg acagcagcct ggccagtatc caagagctcc tgtctcccca
5041 ggagcccccc aggcctcccg aggcagagaa cagcagcccg gattcaggga agcagctggt
5101 gcactacaca gcgcagccgc tgttcctgct ggaccccggc tccgtggaca ccgggagcaa
5161 cgacctgccg gtgctgtttg agctgggaga gggctcctac ttctccgaag gggacggctt
5221 cgccgaggac cccaccatct ccctgctgac aggctcggag cctcccaaag ccaaggaccc
5281 cactgtctcc gctagcggca gtggagaggg cagaggaagt ctgctaacat gcggtgacgt
5341 cgaggagaat cctggcccag tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
5401 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga
5461 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
5521 cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta
5581 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
5641 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
5701 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
5761 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc
5821 cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg
5881 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
5941 gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa
6001 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
6061 cgagctgtac aagtaagaat tcgatatcaa gcttatcgat aatcaacctc tggattacaa
6121 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
6181 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
6241 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
6301 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
6361 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
6421 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
6481 ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat
6541 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
6601 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
6661 tcggatctcc ctttgggccg cctccccgca tcgataccgt cgacctcgag acctagaaaa
6721 acatggagca atcacaagta gcaatacagc agctaccaat gctgattgtg cctggctaga
6781 agcacaagag gaggaggagg tgggttttcc agtcacacct caggtacctt taagaccaat
6841 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
6901 gctaattcac tcccaacgaa gacaagatat ccttgatctg tggatctacc acacacaagg
6961 ctacttccct gattggcaga actacacacc agggccaggg atcagatatc cactgacctt
7021 tggatggtgc tacaagctag taccagttga gcaagagaag gtagaagaag ccaatgaagg
7081 agagaacacc cgcttgttac accctgtgag cctgcatggg atggatgacc cggagagaga
7141 agtattagag tggaggtttg acagccgcct agcatttcat cacatggccc gagagctgca
7201 tccggactgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
7261 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
7321 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
7381 gaaaatctct agcagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt
7441 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
7501 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
7561 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
7621 ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct
7681 ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
7741 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
7801 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
7861 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
7921 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
7981 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
8041 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
8101 tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa
8161 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
8221 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
8281 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
8341 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
8401 cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
8461 ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcagc acgtgttgac
8521 aattaatcat cggcatagta tatcggcata gtataatacg acaaggtgag gaactaaacc
8581 atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc
8641 gagttctgga ccgaccggct cgggttctcc cgggacttcg tggaggacga cttcgccggt
8701 gtggtccggg acgacgtgac cctgttcatc agcgcggtcc aggaccaggt ggtgccggac
8761 aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag
8821 gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca tgaccgagat cggcgagcag
8881 ccgtgggggc gggagttcgc cctgcgcgac ccggccggca actgcgtgca cttcgtggcc
8941 gaggagcagg actgacacgt gctacgagat ttcgattcca ccgccgcctt ctatgaaagg
9001 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
9061 atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa
9121 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
9181 ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc
9241 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
9301 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
9361 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
9421 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
9481 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
9541 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
9601 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
9661 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
9721 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
9781 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
9841 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
9901 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
9961 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
10021 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
10081 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
10141 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
10201 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
10261 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
10321 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
10381 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
10441 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
10501 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
10561 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
10621 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
10681 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
10741 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
10801 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
10861 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
10921 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
10981 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
11041 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
11101 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
11161 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
11221 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
11281 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
11341 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
11401 ccacctgac
//
