pBUE411-GGB

LOCUS        pBUE411-GGB      23358 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Wheat GRF4-GIF1 chimera and pPLTP::BBM cassette in pBUE411.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 23358)
  AUTHORS   Chen Z, Debernardi JM, Dubcovsky J, Gallavotti A
  TITLE     The combination of morphogenic regulators BABY BOOM and GRF-GIF 
            improves maize transformation efficiency
  JOURNAL   bioRxiv 2022.09.02.506370
REFERENCE   2  (bases 1 to 23358)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.biofeng.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2022.09.02.506370"
COMMENT     Sequence Label: pBUE411-GGB
FEATURES             Location/Qualifiers
     source          1..23358
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    21..45
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     terminator      complement(80..332)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(366..455)
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     protein_bind    484..508
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      2417..2426
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(2423..2447)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     4651..4673
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4665..4682
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4666..4682
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(4733..4985)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    4999..5023
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      5064..5073
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     RBS             6210..6221
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     protein_bind    complement(7175..7199)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        8328..10320
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     CDS             10339..10404
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             10411..10431
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             10456..14556
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             10456..14556
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     RBS             14469..14477
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             14557..14604
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     primer_bind     complement(15261..15277)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(15261..15277)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(15274..15296)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    15285..15301
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(15309..15315,15316..15333,15334..15339))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    15354..15375
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        15566..16244
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     16181..16201
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             16289..16840
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     polyA_signal    16847..17021
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    17099..17123
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             17548..18342
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      18429..19017
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     18918..18937
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     19171..19188
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    19203..19343
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(19269..19288)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     19429..19451
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      19687..19881
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(19947..21020)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(21449..22078)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac tgatagtttc
       61 tgcaggcgcg ctaattcccg atctagtaac atagatgaca ccgcgcgcga taatttatcc
      121 tagtttgcgc gctatatttt gttttctatc gcgtattaaa tgtataattg cgggactcta
      181 atcataaaaa cccatctcat aaataacgtc atgcattaca tgttaattat tacatgctta
      241 acgtaattca acagaaatta tatgataatc atcgcaagac cggcaacagg attcaatctt
      301 aagaaacttt attgccaaat gtttgaacga tcggggaaat tcgagctcta agcgctgcac
      361 tgagcagcgt aatctggaac gtcatatgga taggatcctg catagtccgg gacgtcatag
      421 ggatagcccg catagtcagg aacatcgtat gggtaaaaga tgttaattaa cccgctgtta
      481 tcaaccactt tgtacaagaa agctgaacgc tagcttcctt cctcctcggt gcccttgagg
      541 tactccccgt cgccgctgtg cgcgccagag ttggcgccgc gagcgtcggc cgacagcgag
      601 gtcgacccct ccttggcgcc gctgccgccg cggccatagt ccgagaagac gctggcgttc
      661 atcatgctgt tgccagtggc gccgccgccg ccgcccatgc tggcctcacc gtgcaggagg
      721 ttgaacccgg tggtcgcgcc ggaggacatc cccagctggc cgtgcgcggc cgcctggtgc
      781 tgctgctgct gctgctgctg ctggagcggc gacatggcct gctgcgcgta catcatcgac
      841 gaccgcgccg ccatcagcga ctgcggcgac atcatctgcg ccgactgctg ctgcatgtac
      901 cgcggcccgg actgcatcat caggttggac gggtactgag acagcgatgc cgtctgcgga
      961 ggctggctgt cggcgatggc ggcgaggtac atgaggttct gctggagctt agcttggttc
     1021 cgtgcgcact cctccacctt gccgttgttc tggttgtcga ggatggccag gatcagctgc
     1081 ttgttctcat ccaggtactg ctgaatgaga tcagtggtgg cagtggtaga ggaagcgtag
     1141 ccccccatca tgctctggtt catctgcatc aggtgttgct gctgcatggc agcggccgcg
     1201 tacatttcgc cggcgaacag catgccgttg ggcgactggg agctggcggc ggagaagtcg
     1261 gaggacgtca tgggtatcga gatcgacagc tgggtggccg agaaggaggc gacgttcgag
     1321 ttgtcgtcgg tcaggtccga ccacgagtcc ctcgtcttcg gccactcgtc gaagaacggc
     1381 cgcagcgtct ggttctcctg cttcgcggag tcgccgctgc cgaagtcgcc gcaccccggg
     1441 aaggagagcg gctgctgctg ctgctgtccc tcggccttga cgccgctgtt gttgttgctg
     1501 ctgttgttct gacccaggtc actcgttgct ccaagctgag gagagtagct tgagagcggg
     1561 aatgtagtcg tttgagaaga tggcggcagg cgccatgggt tctccacgga ggtgttcatg
     1621 gcttctgtca tgagctgact ctgctcgtct gccaaagatc ttattccata tgcggtatac
     1681 ctgaaatctt tgcatgttcc ggcgccgccg agagccgcgt agggagaggc ggcattgttg
     1741 cccatgtgct gctgaggcgg ccccagcgcg gaggagaacg tgccgggcat ggacatgccg
     1801 ttgttccccc cgcctccacc accgttggtg ccgccgatgg cggggtagag ggagtggttg
     1861 tggaagccgg tggcgagggg cggcacgacg gaggcggccg gcggctggga gtgcgagacg
     1921 agctgcgttt ccacaggctt tcttgaacgg ttgcggccgc ggtgcatgtg gcgctcgcaa
     1981 tacttggagt cggaggcggc ctccttggcg caccgccact tcttgccgtc cgtgcgccgg
     2041 caccggcccg gctccggatc caccttcttg cccaggtacg atccgtaccc gatggcgagg
     2101 gggttgtggt agaagcgggc ggcgagggac tcgatgccgc ggcggatggg gagcacgaga
     2161 tccggcggga cggacacgcc ggccaccagg tacttgtata tgagcgcctg gtgctccagc
     2221 tcctcgtact gcgccgccgt gaagggcacc ggcctcgcca tccacctccc gtccatccgc
     2281 gcctcctccc ccatgccgcc attgccgccg gcggagaagg gggaggagcg gcagaagggg
     2341 aggagggagg cggtggcggt ggcggccggg gaggagcggc ggtcgcctgc cggggaaaga
     2401 gaggcatacg gcatcgccat ggtggcagct tttttgtaca aacttgttga taactctagt
     2461 ctgcagaagt aacaccaaac aacagggtga gcatcgacaa aagaaacagt accaagcaaa
     2521 taaatagcgt atgaaggcag ggctaaaaaa atccacatat agctgctgca tatgccatca
     2581 tccaagtata tcaagatcaa aataattata aaacatactt gtttattata atagataggt
     2641 actcaaggtt agagcatatg aatagatgct gcatatgcca tcatgtatat gcatcagtaa
     2701 aacccacatc aacatgtata cctatcctag atcgatattt ccatccatct taaactcgta
     2761 actatgaaga tgtatgacac acacatacag ttccaaaatt aataaataca ccaggtagtt
     2821 tgaaacagta ttctactccg atctagaacg aatgaacgac cgcccaacca caccacatca
     2881 tcacaaccaa gcgaacaaaa agcatctctg tatatgcatc agtaaaaccc gcatcaacat
     2941 gtatacctat cctagatcga tatttccatc catcatcttc aattcgtaac tatgaatatg
     3001 tatggcacac acatacagat ccaaaattaa taaatccacc aggtagtttg aaacagaatt
     3061 ctactccgat ctagaacgac cgcccaacca gaccacatca tcacaaccaa gacaaaaaaa
     3121 agcatgaaaa gatgacccga caaacaagtg cacggcatat attgaaataa aggaaaaggg
     3181 caaaccaaac cctatgcaac gaaacaaaaa aaatcatgaa atcgatcccg tctgcggaac
     3241 ggctagagcc atcccaggat tccccaaaga gaaacactgg caagttagca atcagaacgt
     3301 gtctgacgta caggtcgcat ccgtgtacga acgctagcag cacggatcta acacaaacac
     3361 ggatctaaca caaacatgaa cagaagtaga actaccgggc cctaaccatg gaccggaacg
     3421 ccgatctaga gaaggkagag aggggggggg ggggaggacg agcggcgtac cttgaagcgg
     3481 aggtgccgac gggtggattt gggggagatc tggttgtgtg tgtgtgcgct ccgaacaaca
     3541 cgaggttggg gaaagagggt gtggaggggg tgtctattta ttacggcggg cgaggaaggg
     3601 aaagcgaagg agcggtggga aaggaatccc ccgtagctgc cggtgccgtg agaggaggag
     3661 gaggccgcct gccgtgccgg ctcacgtctg ccgctccgcc acgcaatttc tggatgccga
     3721 cagcggagca agtccaacgg tggagcggaa ctctcgagag gggtccagag gcagcgacag
     3781 agatgccgtg ccgtctgctt cgcttggccc gacgcgacgc tgctggttcg ctggttggtg
     3841 tccgttagac tcgtcgacgg cgtttaacag gctggcatta tctactcgaa acaagaaaaa
     3901 tgtttcctta gtttttttaa tttcttaaag ggtatttgtt taatttttag tcactttatt
     3961 ttattctatt ttatatctaa attattaaat aaaaaaacta aaatagagtt ttagttttct
     4021 taatttagag gctaaaatag aataaaatag atgtactaaa aaaattagtc tataaaaacc
     4081 attaacccta aaccctaaat ggatgtacta ataaaatgga tgaagtatta tataggtgaa
     4141 gctatttgca aaaaaaaagg agaacacatg cacactaaaa agataaaact gtagagtcct
     4201 gttgtcaaaa tactcaattg tcctttagac catgtctaac tgttcattta tatgattctc
     4261 taaaacactg atattattgt agtactatag attatattat tcgtagagta aagtttaaat
     4321 atatgtataa agatagataa actgcacttc aaacaagtgt gacaaaaaaa atatgtggta
     4381 attttttata acttagacat gcaatgctca ttatctctag agaggggcac gaccgggtca
     4441 cgctgcacgc gcctgcagat ccccgggtac cgagctcaaa ctgaaggcgg gaaacgacaa
     4501 tctgatccaa gctcaagctg ctctagcatt cgccattcag gctgcgcaac tgttgggaag
     4561 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
     4621 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
     4681 gtgccaagct atggtgagac ctcggtctcg ctccactagt tcggtacccg gggatctagt
     4741 aacatagatg acaccgcgcg cgataattta tcctagtttg cgcgctatat tttgttttct
     4801 atcgcgtatt aaatgtataa ttgcgggact ctaatcataa aaacccatct cataaataac
     4861 gtcatgcatt acatgttaat tattacatgc ttaacgtaat tcaacagaaa ttatatgata
     4921 atcatcgcaa gaccggcaac aggattcaat cttaagaaac tttattgcca aatgtttgaa
     4981 cgatcttaat taaatcaaac cactttgtac aagaaagctg ggcccatgag gccagtgtcg
     5041 ttccagacac tgaagagctg cgcgccgcca tggccgacgt cggcggcaat gttgtcgttg
     5101 ctgcttgctg ctgccgccgc ggctgcagag acgacggtcc cccatgcaga catccttccg
     5161 ccaccattgt tctccgcgtt caccaggtag ctctcgtacc ccatctgagc agcctgcttg
     5221 gcttcgtcgt aggcccgtgc atgcatctgc tcgtggctca ccattgccga tgtagtggtt
     5281 gctccggcag cgctcatcgg catcatgtag ccaccgccgc tgccaacggc gctggcgccg
     5341 ttgctatcgc cgaccccgcc gttgtagacg acggagttgg agccggtgct gtgctcgagc
     5401 gacgcactgt cgatgctagc caggccgtgc atcgcagctg cggcggcggc ctgctgccct
     5461 gccgagaaaa agtcgtgcgc gccggccgcg cccaggttca agtggtggag gtcctgcagg
     5521 ctgtgcgcgg ccgcgatcac cgcgtggtcc tgctcctgct tgcaccaccc gccgccgcgc
     5581 attggctgct gcgcgtacgg gtggtacagg cctgtggtgg cggcgcccgg ctggaacgcg
     5641 atggtcggcc aggcggcgcc gtggtagtgc gcgccgtacg ccgccgctag ggctccgccg
     5701 tcgccgagct gcgaggcgat gcggccgacg tcgtagctca ccacgccggc gtggtggtgc
     5761 tgcgcggacg ctgcggcctc ggcctccttg agacgcttgg cggcgctgcc gatggggagg
     5821 gcgctgctgt ccaggatgct cttcacgtcg tagcggctca tgtcgaagtt ggtgacggcg
     5881 ttgaggccgc ggaacttgat cgccgcgatg tcgtacgcct ccgctgcctc ctcctgggtg
     5941 ctgaaggtgc ccaagtaaag atccttgttc cctgcaactc gtccaatccg tgcttgccat
     6001 cttccatgtt ggtgatgcct agtcactccc ctgtaaatgg atgcacctct ggagaaacca
     6061 ctgctcttcc ttctcagaga cgctacaaac tcctgccttg tcatgtgctt catgtcctcg
     6121 agctcctttt cgtagttact cactggaaaa tttgttgttg ttgtggcacc ccagtacttc
     6181 agagcagcaa gatcataagc cctagcagct ttctcctctt tatcatagcc acctaaatag
     6241 acttgacgac ccttacgagt ttgtccttcc cttctgcaac tgttatccca aagatgtgcc
     6301 tcatatctcc cagtccatct atgccttgtc acgccacggt aaatcgacgt gcgctgcccg
     6361 aacgtgtcca ccgtcttcct tgccgtgttg tcagccgacg cgccgccgct gccacccgtg
     6421 tccgtgctca cggctactag agcaccggca acaccgctgc caccgctatc ctccttcggc
     6481 gccgtgacga cgacggcacc accctgtgct gacgtcgata cactctcggg cgcccgtgcg
     6541 cgctctccag cgagaagtgg catgccagca gcgccttggg tcgtccccgc catgttcatg
     6601 gacaaagaga gcccctgcgc gccctcagcc gccgccgccc tcggctgcat gggcgccggt
     6661 tggctccgca gccagttctt gatcatggac agcccgatgc cgccgccgtt ggcactggca
     6721 gcgccagcga caccgttagc ggcggccgcg ctgagcatgg aaccgccgtc gtggacaccg
     6781 gccgaggagg ccaccatgac ggagtccgcg aagtggagcg cggagctggt gggctggtac
     6841 agctggtgat ggtagccggt gctagcagct gagctcgcgt agcaaactgt gctgctagtg
     6901 ctgggtatca agttgcagtt ggacttgtga tgctgctcgg agaaggagat gccgccgagg
     6961 aagtcctcca gcttcggctc cgcgacgagc gccgaaagct ctgatcccct catgctccaa
     7021 tcttggggga tgttgaagca gacatcgccg gagacatggt cggcggtggc ggccgagatg
     7081 agcgtggagt ccgtcgtctg ggagggcggc agctcctgcg gggagaggga gaaagcgagc
     7141 cagttgttca cagtggccat tggccttgac ggcccttctg cttttttgta caaacttgtt
     7201 tgatggatcc gttccttgag gagggaggtg cctgccgctc tgctagctcg tcgggctgtt
     7261 tggaatgcgg cggtcggtag atggcaagtg ctttgctaga gctagctggt gatgagtggt
     7321 gaggagagtt gcagtgggat gtcgctcctt aaataggcct gtgtacgtgt gatcggtgga
     7381 aaatggaaat aatggtggat ggatggatgc acgcgtatcg gcgtatggat gcaagcttgc
     7441 atggtggatg ttccgtcgtc gtcgtcgtct tcttccttgg atggcatcga tccgtgtcca
     7501 agttattaag agccgagcag gaggtggttg ggtgttggag ggaggcatgt acagtatata
     7561 tgcagagtta attcatcatc agcgcagtag tagtatacta actggctaga ctagatactc
     7621 ctatgtagct ggcgccgcct aacgcgactt tcgagcaggg tgatggcggc agcctgagcc
     7681 ggccggcaga cgtcgtatac gtacgtggct catctgcggc ggcagtcgtc ggatgtctct
     7741 ctttctctct atcagtgtgt cagtgtggag gtagtttgcg gttggatctg agtgcttatc
     7801 gcgcaattaa cacgtacatc ctccggccac ctacattaag agacaccaca tccatcatcc
     7861 atggacgatg cttccagtag tcttcgtcct cgtcccttcc tcccactttc ctccttagag
     7921 cgagtttaat taataataat aataatagag cctatatatt attagctcta actccttacc
     7981 atatcatcta aagccaacaa taaacacatt catataataa agttggctct tagataaatc
     8041 tactctcttc ctgtttcatt attttgtctt gaagctcttt tagcaccggg ccgtccgccg
     8101 ttgcatgaga accactctct ctaattttta tatgtctctt ccacaataaa aaatgtcatg
     8161 taagcagact taaaacctat tatcatacat actcttgcac tgttacacag gctacaaccg
     8221 caagacagag attgatgggg gacgcgtaca gcgcaggagc taggttacat gcacattcaa
     8281 aaaattcaag tagcagcagt agcagcgtgg cgcgccagct tgcatgcctg cagtgcagcg
     8341 tgacccggtc gtgcccctct ctagagataa tgagcattgc atgtctaagt tataaaaaat
     8401 taccacatat tttttttgtc acacttgttt gaagtgcagt ttatctatct ttatacatat
     8461 atttaaactt tactctacga ataatataat ctatagtact acaataatat cagtgtttta
     8521 gagaatcata taaatgaaca gttagacatg gtctaaagga caattgagta ttttgacaac
     8581 aggactctac agttttatct ttttagtgtg catgtgttct cctttttttt tgcaaatagc
     8641 ttcacctata taatacttca tccattttat tagtacatcc atttagggtt tagggttaat
     8701 ggtttttata gactaatttt tttagtacat ctattttatt ctattttagc ctctaaatta
     8761 agaaaactaa aactctattt tagttttttt atttaataat ttagatataa aatagaataa
     8821 aataaagtga ctaaaaatta aacaaatacc ctttaagaaa ttaaaaaaac taaggaaaca
     8881 tttttcttgt ttcgagtaga taatgccagc ctgttaaacg ccgtcgacga gtctaacgga
     8941 caccaaccag cgaaccagca gcgtcgcgtc gggccaagcg aagcagacgg cacggcatct
     9001 ctgtcgctgc ctctggaccc ctctcgagag ttccgctcca ccgttggact tgctccgctg
     9061 tcggcatcca gaaattgcgt ggcggagcgg cagacgtgag ccggcacggc aggcggcctc
     9121 ctcctcctct cacggcaccg gcagctacgg gggattcctt tcccaccgct ccttcgcttt
     9181 cccttcctcg cccgccgtaa taaatagaca ccccctccac accctctttc cccaacctcg
     9241 tgttgttcgg agcgcacaca cacacaacca gatctccccc aaatccaccc gtcggcacct
     9301 ccgcttcaag gtacgccgct cgtcctcccc ccccccccct ctctaccttc tctagatcgg
     9361 cgttccggtc catggttagg gcccggtagt tctacttctg ttcatgtttg tgttagatcc
     9421 gtgtttgtgt tagatccgtg ctgctagcgt tcgtacacgg atgcgacctg tacgtcagac
     9481 acgttctgat tgctaacttg ccagtgtttc tctttgggga atcctgggat ggctctagcc
     9541 gttccgcaga cgggatcgat ttcatgattt tttttgtttc gttgcatagg gtttggtttg
     9601 cccttttcct ttatttcaat atatgccgtg cacttgtttg tcgggtcatc ttttcatgct
     9661 tttttttgtc ttggttgtga tgatgtggtc tggttgggcg gtcgttctag atcggagtag
     9721 aattctgttt caaactacct ggtggattta ttaattttgg atctgtatgt gtgtgccata
     9781 catattcata gttacgaatt gaagatgatg gatggaaata tcgatctagg ataggtatac
     9841 atgttgatgc gggttttact gatgcatata cagagatgct ttttgttcgc ttggttgtga
     9901 tgatgtggtg tggttgggcg gtcgttcatt cgttctagat cggagtagaa tactgtttca
     9961 aactacctgg tgtatttatt aattttggaa ctgtatgtgt gtgtcataca tcttcatagt
    10021 tacgagttta agatggatgg aaatatcgat ctaggatagg tatacatgtt gatgtgggtt
    10081 ttactgatgc atatacatga tggcatatgc agcatctatt catatgctct aaccttgagt
    10141 acctatctat tataataaac aagtatgttt tataattatt ttgatcttga tatacttgga
    10201 tgatggcata tgcagcagct atatgtggat ttttttagcc ctgccttcat acgctattta
    10261 tttgcttggt actgtttctt ttgtcgatgc tcaccctgtt gtttggtgtt acttctgcag
    10321 ccctaggcct actagatgga ttacaaggac cacgacgggg attacaagga ccacgacatt
    10381 gattacaagg atgatgatga caagatggct ccgaagaaga agaggaaggt tggcatccac
    10441 ggggtgccag ctgctgacaa gaagtactcg atcggcctcg atattgggac taactctgtt
    10501 ggctgggccg tgatcaccga cgagtacaag gtgccctcaa agaagttcaa ggtcctgggc
    10561 aacaccgatc ggcattccat caagaagaat ctcattggcg ctctcctgtt cgacagcggc
    10621 gagacggctg aggctacgcg gctcaagcgc accgcccgca ggcggtacac gcgcaggaag
    10681 aatcgcatct gctacctgca ggagattttc tccaacgaga tggcgaaggt tgacgattct
    10741 ttcttccaca ggctggagga gtcattcctc gtggaggagg ataagaagca cgagcggcat
    10801 ccaatcttcg gcaacattgt cgacgaggtt gcctaccacg agaagtaccc tacgatctac
    10861 catctgcgga agaagctcgt ggactccaca gataaggcgg acctccgcct gatctacctc
    10921 gctctggccc acatgattaa gttcaggggc catttcctga tcgaggggga tctcaacccg
    10981 gacaatagcg atgttgacaa gctgttcatc cagctcgtgc agacgtacaa ccagctcttc
    11041 gaggagaacc ccattaatgc gtcaggcgtc gacgcgaagg ctatcctgtc cgctaggctc
    11101 tcgaagtctc ggcgcctcga gaacctgatc gcccagctgc cgggcgagaa gaagaacggc
    11161 ctgttcggga atctcattgc gctcagcctg gggctcacgc ccaacttcaa gtcgaatttc
    11221 gatctcgctg aggacgccaa gctgcagctc tccaaggaca catacgacga tgacctggat
    11281 aacctcctgg cccagatcgg cgatcagtac gcggacctgt tcctcgctgc caagaatctg
    11341 tcggacgcca tcctcctgtc tgatattctc agggtgaaca ccgagattac gaaggctccg
    11401 ctctcagcct ccatgatcaa gcgctacgac gagcaccatc aggatctgac cctcctgaag
    11461 gcgctggtca ggcagcagct ccccgagaag tacaaggaga tcttcttcga tcagtcgaag
    11521 aacggctacg ctgggtacat tgacggcggg gcctctcagg aggagttcta caagttcatc
    11581 aagccgattc tggagaagat ggacggcacg gaggagctgc tggtgaagct caatcgcgag
    11641 gacctcctga ggaagcagcg gacattcgat aacggcagca tcccacacca gattcatctc
    11701 ggggagctgc acgctatcct gaggaggcag gaggacttct accctttcct caaggataac
    11761 cgcgagaaga tcgagaagat tctgactttc aggatcccgt actacgtcgg cccactcgct
    11821 aggggcaact cccgcttcgc ttggatgacc cgcaagtcag aggagacgat cacgccgtgg
    11881 aacttcgagg aggtggtcga caagggcgct agcgctcagt cgttcatcga gaggatgacg
    11941 aatttcgaca agaacctgcc aaatgagaag gtgctcccta agcactcgct cctgtacgag
    12001 tacttcacag tctacaacga gctgactaag gtgaagtatg tgaccgaggg catgaggaag
    12061 ccggctttcc tgtctgggga gcagaagaag gccatcgtgg acctcctgtt caagaccaac
    12121 cggaaggtca cggttaagca gctcaaggag gactacttca agaagattga gtgcttcgat
    12181 tcggtcgaga tctctggcgt tgaggaccgc ttcaacgcct ccctggggac ctaccacgat
    12241 ctcctgaaga tcattaagga taaggacttc ctggacaacg aggagaatga ggatatcctc
    12301 gaggacattg tgctgacact cactctgttc gaggaccggg agatgatcga ggagcgcctg
    12361 aagacttacg cccatctctt cgatgacaag gtcatgaagc agctcaagag gaggaggtac
    12421 accggctggg ggaggctgag caggaagctc atcaacggca ttcgggacaa gcagtccggg
    12481 aagacgatcc tcgacttcct gaagagcgat ggcttcgcga accgcaattt catgcagctg
    12541 attcacgatg acagcctcac attcaaggag gatatccaga aggctcaggt gagcggccag
    12601 ggggactcgc tgcacgagca tatcgcgaac ctcgctggct cgccagctat caagaagggg
    12661 attctgcaga ccgtgaaggt tgtggacgag ctggtgaagg tcatgggcag gcacaagcct
    12721 gagaacatcg tcattgagat ggcccgggag aatcagacca cgcagaaggg ccagaagaac
    12781 tcacgcgaga ggatgaagag gatcgaggag ggcattaagg agctggggtc ccagatcctc
    12841 aaggagcacc cggtggagaa cacgcagctg cagaatgaga agctctacct gtactacctc
    12901 cagaatggcc gcgatatgta tgtggaccag gagctggata ttaacaggct cagcgattac
    12961 gacgtcgatc atatcgttcc acagtcattc ctgaaggatg actccattga caacaaggtc
    13021 ctcaccaggt cggacaagaa ccggggcaag tctgataatg ttccttcaga ggaggtcgtt
    13081 aagaagatga agaactactg gcgccagctc ctgaatgcca agctgatcac gcagcggaag
    13141 ttcgataacc tcacaaaggc tgagaggggc gggctctctg agctggacaa ggcgggcttc
    13201 atcaagaggc agctggtcga gacacggcag atcactaagc acgttgcgca gattctcgac
    13261 tcacggatga acactaagta cgatgagaat gacaagctga tccgcgaggt gaaggtcatc
    13321 accctgaagt caaagctcgt ctccgacttc aggaaggatt tccagttcta caaggttcgg
    13381 gagatcaaca attaccacca tgcccatgac gcgtacctga acgcggtggt cggcacagct
    13441 ctgatcaaga agtacccaaa gctcgagagc gagttcgtgt acggggacta caaggtttac
    13501 gatgtgagga agatgatcgc caagtcggag caggagattg gcaaggctac cgccaagtac
    13561 ttcttctact ctaacattat gaatttcttc aagacagaga tcactctggc caatggcgag
    13621 atccggaagc gccccctcat cgagacgaac ggcgagacgg gggagatcgt gtgggacaag
    13681 ggcagggatt tcgcgaccgt caggaaggtt ctctccatgc cacaagtgaa tatcgtcaag
    13741 aagacagagg tccagactgg cgggttctct aaggagtcaa ttctgcctaa gcggaacagc
    13801 gacaagctca tcgcccgcaa gaaggactgg gatccgaaga agtacggcgg gttcgacagc
    13861 cccactgtgg cctactcggt cctggttgtg gcgaaggttg agaagggcaa gtccaagaag
    13921 ctcaagagcg tgaaggagct gctggggatc acgattatgg agcgctccag cttcgagaag
    13981 aacccgatcg atttcctgga ggcgaagggc tacaaggagg tgaagaagga cctgatcatt
    14041 aagctcccca agtactcact cttcgagctg gagaacggca ggaagcggat gctggcttcc
    14101 gctggcgagc tgcagaaggg gaacgagctg gctctgccgt ccaagtatgt gaacttcctc
    14161 tacctggcct cccactacga gaagctcaag ggcagccccg aggacaacga gcagaagcag
    14221 ctgttcgtcg agcagcacaa gcattacctc gacgagatca ttgagcagat ttccgagttc
    14281 tccaagcgcg tgatcctggc cgacgcgaat ctggataagg tcctctccgc gtacaacaag
    14341 caccgcgaca agccaatcag ggagcaggct gagaatatca ttcatctctt caccctgacg
    14401 aacctcggcg cccctgctgc tttcaagtac ttcgacacaa ctatcgatcg caagaggtac
    14461 acaagcacta aggaggtcct ggacgcgacc ctcatccacc agtcgattac cggcctctac
    14521 gagacgcgca tcgacctgtc tcagctcggg ggcgacaagc ggccagcggc gacgaagaag
    14581 gcggggcagg cgaagaagaa gaagtgagct cagagctttc gttcgtatca tcggtttcga
    14641 caacgttcgt caagttcaat gcatcagttt cattgcgcac acaccagaat cctactgagt
    14701 ttgagtatta tggcattggg aaaactgttt ttcttgtacc atttgttgtg cttgtaattt
    14761 actgtgtttt ttattcggtt ttcgctatcg aactgtgaaa tggaaatgga tggagaagag
    14821 ttaatgaatg atatggtcct tttgttcatt ctcaaattaa tattatttgt tttttctctt
    14881 atttgttgtg tgttgaattt gaaattataa gagatatgca aacattttgt tttgagtaaa
    14941 aatgtgtcaa atcgtggcct ctaatgaccg aagttaatat gaggagtaaa acacttgtag
    15001 ttgtaccatt atgcttattc actaggcaac aaatatattt tcagacctag aaaagctgca
    15061 aatgttactg aatacaagta tgtcctcttg tgttttagac atttatgaac tttcctttat
    15121 gtaattttcc agaatccttg tcagattcta atcattgctt tataattata gttatactca
    15181 tggatttgta gttgagtatg aaaatatttt ttaatgcatt ttatgacttg ccaattgatt
    15241 gacaacgaat tcgtaatcat gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
    15301 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
    15361 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
    15421 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattggctag
    15481 agcagcttgc caacatggtg gagcacgaca ctctcgtcta ctccaagaat atcaaagata
    15541 cagtctcaga agaccaaagg gctattgaga cttttcaaca aagggtaata tcgggaaacc
    15601 tcctcggatt ccattgccca gctatctgtc acttcatcaa aaggacagta gaaaaggaag
    15661 gtggcaccta caaatgccat cattgcgata aaggaaaggc tatcgttcaa gatgcctctg
    15721 ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg
    15781 ttccaaccac gtcttcaaag cggctagagc agcttgccaa catggtggag cacgacactc
    15841 tcgtctactc caagaatatc aaagatacag tctcagaaga ccaaagggct attgagactt
    15901 ttcaacaaag ggtaatatcg ggaaacctcc tcggattcca ttgcccagct atctgtcact
    15961 tcatcaaaag gacagtagaa aaggaaggtg gcacctacaa atgccatcat tgcgataaag
    16021 gaaaggctat cgttcaagat gcctctgccg acagtggtcc caaagatgga cccccaccca
    16081 cgaggagcat cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat
    16141 gtgatatctc cactgacgta agggatgacg cacaatccca ctatccttcg caagaccctt
    16201 cctctatata aggaagttca tttcatttgg agaggacacg ctgaaatcac cagtctctct
    16261 ctacaaatct atctctctcg agtctaccat gagcccagaa cgacgcccgg ccgacatccg
    16321 ccgtgccacc gaggcggaca tgccggcggt ctgcaccatc gtcaaccact acatcgagac
    16381 aagcacggtc aacttccgta ccgagccgca ggaaccgcag gagtggacgg acgacctcgt
    16441 ccgtctgcgg gagcgctatc cctggctcgt cgccgaggtg gacggcgagg tcgccggcat
    16501 cgcctacgcg ggcccctgga aggcacgcaa cgcctacgac tggacggccg agtcgaccgt
    16561 gtacgtctcc ccccgccacc agcggacggg actgggctcc acgctctaca cccacctgct
    16621 gaagtccctg gaggcacagg gcttcaagag cgtggtcgct gtcatcgggc tgcccaacga
    16681 cccgagcgtg cgcatgcacg aggcgctcgg atatgccccc cgcggcatgc tgcgggcggc
    16741 cggcttcaag cacgggaact ggcatgacgt gggtttctgg cagctggact tcagcctgcc
    16801 ggtaccgccc cgtccggtcc tgcccgtcac cgagatttga ctcgagtttc tccataataa
    16861 tgtgtgagta gttcccagat aagggaatta gggttcctat agggtttcgc tcatgtgttg
    16921 agcatataag aaacccttag tatgtatttg tatttgtaaa atacttctat caataaaatt
    16981 tctaattcct aaaaccaaaa tccagtacta aaatccagat cccccgaatt aattcggcgt
    17041 taattcagta cattaaaaac gtccgcaatg tgttattaag ttgtctaagc gtcaatttgt
    17101 ttacaccaca atatatcctg ccaccagcca gccaacagct ccccgaccgg cagctcggca
    17161 caaaatcacc actcgataca ggcagcccat cagtccggga cggcgtcagc gggagagccg
    17221 ttgtaaggcg gcagactttg ctcatgttac cgatgctatt cggaagaacg gcaactaagc
    17281 tgccgggttt gaaacacgga tgatctcgcg gagggtagca tgttgattgt aacgatgaca
    17341 gagcgttgct gcctgtgatc accgcggttt caaaatcggc tccgtcgata ctatgttata
    17401 cgccaacttt gaaaacaact ttgaaaaagc tgttttctgg tatttaaggt tttagaatgc
    17461 aaggaacagt gaattggagt tcgtcttgtt ataattagct tcttggggta tctttaaata
    17521 ctgtagaaaa gaggaaggaa ataataaatg gctaaaatga gaatatcacc ggaattgaaa
    17581 aaactgatcg aaaaataccg ctgcgtaaaa gatacggaag gaatgtctcc tgctaaggta
    17641 tataagctgg tgggagaaaa tgaaaaccta tatttaaaaa tgacggacag ccggtataaa
    17701 gggaccacct atgatgtgga acgggaaaag gacatgatgc tatggctgga aggaaagctg
    17761 cctgttccaa aggtcctgca ctttgaacgg catgatggct ggagcaatct gctcatgagt
    17821 gaggccgatg gcgtcctttg ctcggaagag tatgaagatg aacaaagccc tgaaaagatt
    17881 atcgagctgt atgcggagtg catcaggctc tttcactcca tcgacatatc ggattgtccc
    17941 tatacgaata gcttagacag ccgcttagcc gaattggatt acttactgaa taacgatctg
    18001 gccgatgtgg attgcgaaaa ctgggaagaa gacactccat ttaaagatcc gcgcgagctg
    18061 tatgattttt taaagacgga aaagcccgaa gaggaacttg tcttttccca cggcgacctg
    18121 ggagacagca acatctttgt gaaagatggc aaagtaagtg gctttattga tcttgggaga
    18181 agcggcaggg cggacaagtg gtatgacatt gccttctgcg tccggtcgat cagggaggat
    18241 atcggggaag aacagtatgt cgagctattt tttgacttac tggggatcaa gcctgattgg
    18301 gagaaaataa aatattatat tttactggat gaattgtttt agtacctaga atgcatgacc
    18361 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
    18421 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
    18481 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
    18541 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
    18601 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
    18661 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
    18721 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
    18781 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
    18841 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
    18901 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
    18961 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
    19021 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
    19081 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
    19141 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
    19201 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc
    19261 actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc
    19321 tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac
    19381 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
    19441 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagggtgcc ttgatgtggg
    19501 cgccggcggt cgagtggcga cggcgcggct tgtccgcgcc ctggtagatt gcctggccgt
    19561 aggccagcca tttttgagcg gccagcggcc gcgataggcc gacgcgaagc ggcggggcgt
    19621 agggagcgca gcgaccgaag ggtaggcgct ttttgcagct cttcggctgt gcgctggcca
    19681 gacagttatg cacaggccag gcgggtttta agagttttaa taagttttaa agagttttag
    19741 gcggaaaaat cgcctttttt ctcttttata tcagtcactt acatgtgtga ccggttccca
    19801 atgtacggct ttgggttccc aatgtacggg ttccggttcc caatgtacgg ctttgggttc
    19861 ccaatgtacg tgctatccac aggaaacaga ccttttcgac ctttttcccc tgctagggca
    19921 atttgcccta gcatctgctc cgtacattag gaaccggcgg atgcttcgcc ctcgatcagg
    19981 ttgcggtagc gcatgactag gatcgggcca gcctgccccg cctcctcctt caaatcgtac
    20041 tccggcaggt catttgaccc gatcagcttg cgcacggtga aacagaactt cttgaactct
    20101 ccggcgctgc cactgcgttc gtagatcgtc ttgaacaacc atctggcttc tgccttgcct
    20161 gcggcgcggc gtgccaggcg gtagagaaaa cggccgatgc cgggatcgat caaaaagtaa
    20221 tcggggtgaa ccgtcagcac gtccgggttc ttgccttctg tgatctcgcg gtacatccaa
    20281 tcagctagct cgatctcgat gtactccggc cgcccggttt cgctctttac gatcttgtag
    20341 cggctaatca aggcttcacc ctcggatacc gtcaccaggc ggccgttctt ggccttcttc
    20401 gtacgctgca tggcaacgtg cgtggtgttt aaccgaatgc aggtttctac caggtcgtct
    20461 ttctgctttc cgccatcggc tcgccggcag aacttgagta cgtccgcaac gtgtggacgg
    20521 aacacgcggc cgggcttgtc tcccttccct tcccggtatc ggttcatgga ttcggttaga
    20581 tgggaaaccg ccatcagtac caggtcgtaa tcccacacac tggccatgcc ggccggccct
    20641 gcggaaacct ctacgtgccc gtctggaagc tcgtagcgga tcacctcgcc agctcgtcgg
    20701 tcacgcttcg acagacggaa aacggccacg tccatgatgc tgcgactatc gcgggtgccc
    20761 acgtcataga gcatcggaac gaaaaaatct ggttgctcgt cgcccttggg cggcttccta
    20821 atcgacggcg caccggctgc cggcggttgc cgggattctt tgcggattcg atcagcggcc
    20881 gcttgccacg attcaccggg gcgtgcttct gcctcgatgc gttgccgctg ggcggcctgc
    20941 gcggccttca acttctccac caggtcatca cccagcgccg cgccgatttg taccgggccg
    21001 gatggtttgc gaccgctcac gccgattcct cgggcttggg ggttccagtg ccattgcagg
    21061 gccggcaggc aacccagccg cttacgcctg gccaaccgcc cgttcctcca cacatggggc
    21121 attccacggc gtcggtgcct ggttgttctt gattttccat gccgcctcct ttagccgcta
    21181 aaattcatct actcatttat tcatttgctc atttactctg gtagctgcgc gatgtattca
    21241 gatagcagct cggtaatggt cttgccttgg cgtaccgcgt acatcttcag cttggtgtga
    21301 tcctccgccg gcaactgaaa gttgacccgc ttcatggctg gcgtgtctgc caggctggcc
    21361 aacgttgcag ccttgctgct gcgtgcgctc ggacggccgg cacttagcgt gtttgtgctt
    21421 ttgctcattt tctctttacc tcattaactc aaatgagttt tgatttaatt tcagcggcca
    21481 gcgcctggac ctcgcgggca gcgtcgccct cgggttctga ttcaagaacg gttgtgccgg
    21541 cggcggcagt gcctgggtag ctcacgcgct gcgtgatacg ggactcaaga atgggcagct
    21601 cgtacccggc cagcgcctcg gcaacctcac cgccgatgcg cgtgcctttg atcgcccgcg
    21661 acacgacaaa ggccgcttgt agccttccat ccgtgacctc aatgcgctgc ttaaccagct
    21721 ccaccaggtc ggcggtggcc catatgtcgt aagggcttgg ctgcaccgga atcagcacga
    21781 agtcggctgc cttgatcgcg gacacagcca agtccgccgc ctggggcgct ccgtcgatca
    21841 ctacgaagtc gcgccggccg atggccttca cgtcgcggtc aatcgtcggg cggtcgatgc
    21901 cgacaacggt tagcggttga tcttcccgca cggccgccca atcgcgggca ctgccctggg
    21961 gatcggaatc gactaacaga acatcggccc cggcgagttg cagggcgcgg gctagatggg
    22021 ttgcgatggt cgtcttgcct gacccgcctt tctggttaag tacagcgata accttcatgc
    22081 gttccccttg cgtatttgtt tatttactca tcgcatcata tacgcagcga ccgcatgacg
    22141 caagctgttt tactcaaata cacatcacct ttttagacgg cggcgctcgg tttcttcagc
    22201 ggccaagctg gccggccagg ccgccagctt ggcatcagac aaaccggcca ggatttcatg
    22261 cagccgcacg gttgagacgt gcgcgggcgg ctcgaacacg tacccggccg cgatcatctc
    22321 cgcctcgatc tcttcggtaa tgaaaaacgg ttcgtcctgg ccgtcctggt gcggtttcat
    22381 gcttgttcct cttggcgttc attctcggcg gccgccaggg cgtcggcctc ggtcaatgcg
    22441 tcctcacgga aggcaccgcg ccgcctggcc tcggtgggcg tcacttcctc gctgcgctca
    22501 agtgcgcggt acagggtcga gcgatgcacg ccaagcagtg cagccgcctc tttcacggtg
    22561 cggccttcct ggtcgatcag ctcgcgggcg tgcgcgatct gtgccggggt gagggtaggg
    22621 cgggggccaa acttcacgcc tcgggccttg gcggcctcgc gcccgctccg ggtgcggtcg
    22681 atgattaggg aacgctcgaa ctcggcaatg ccggcgaaca cggtcaacac catgcggccg
    22741 gccggcgtgg tggtgtcggc ccacggctct gccaggctac gcaggcccgc gccggcctcc
    22801 tggatgcgct cggcaatgtc cagtaggtcg cgggtgctgc gggccaggcg gtctagcctg
    22861 gtcactgtca caacgtcgcc agggcgtagg tggtcaagca tcctggccag ctccgggcgg
    22921 tcgcgcctgg tgccggtgat cttctcggaa aacagcttgg tgcagccggc cgcgtgcagt
    22981 tcggcccgtt ggttggtcaa gtcctggtcg tcggtgctga cgcgggcata gcccagcagg
    23041 ccagcggcgg cgctcttgtt catggcgtaa tgtctccggt tctagtcgca agtattctac
    23101 tttatgcgac taaaacacgc gacaagaaaa cgccaggaaa agggcagggc ggcagcctgt
    23161 cgcgtaactt aggacttgtg cgacatgtcg ttttcagaag acggctgcac tgaacgtcag
    23221 aagccgactg cactatagca gcggaggggt tggatcaaag tactttgatc ccgaggggaa
    23281 ccctgtggtt ggcatgcaca tacaaatgga cgaacggata aaccttttca cgccctttta
    23341 aatatccgat tattctaa
//