pCambia1300-bk1-EYFP-bk2

LOCUS       pCambia1300-bk1-EYFP-bk2              12578 bp DNA     circular SYN 24-MAR-2026
DEFINITION  pCAMBIA1300-nLUC.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12578)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 24, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..12578
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             337..1404
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      1470..1664
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     misc_feature    2008..2148
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(2334..2922)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4347..5141)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    5566..5590
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    5668..5842
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(5883..6908)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDDLMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(6975..7651)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(7330..7347)
                     /label=seq
     primer_bind     complement(7655..7672)
                     /label=seq
     protein_bind    7842..7863
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7878..7908
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7916..7932
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7940..7956
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8444..8461
                     /label=seq
     promoter        8465..8810
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             8863..8865
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="M"
     CDS             8866..9579
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     misc_feature    9586..9606
                     /label=同源臂
     misc_feature    9619..9639
                     /label=同源臂
     CDS             9640..9648
                     /codon_start=1
                     /label=Feature 22
                     /translation="EFP"
     terminator      9658..10299
                     /label=E9 terminator
                     /note="terminator and polyadenylation signal from the pea 
                     rbcS-E9 gene"
     primer_bind     complement(10307..10323)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    10526..10550
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             11851..12480
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 gcagacacgc cagccatgaa gcgggtcaac tttcagttgc cggcggagga tcacaccaag
       61 ctgaagatgt acgcggtacg ccaaggcaag accattaccg agctgctatc tgaatacatc
      121 gcgcagctac cagagtaaat gagcaaatga ataaatgagt agatgaattt tagcggctaa
      181 aggaggcggc atggaaaatc aagaacaacc aggcaccgac gccgtggaat gccccatgtg
      241 tggaggaacg ggcggttggc caggcgtaag cggctgggtt gcctgccggc cctgcaatgg
      301 cactggaacc cccaagcccg aggaatcggc gtgagcggtc gcaaaccatc cggcccggta
      361 caaatcggcg cggcgctggg tgatgacctg gtggagaagt tgaaggccgc gcaggccgcc
      421 cagcggcaac gcatcgaggc agaagcacgc cccggtgaat cgtggcaagc ggccgctgat
      481 cgaatccgca aagaatcccg gcaaccgccg gcagccggtg cgccgtcgat taggaagccg
      541 cccaagggcg acgagcaacc agattttttc gttccgatgc tctatgacgt gggcacccgc
      601 gatagtcgca gcatcatgga cgtggccgtt ttccgtctgt cgaagcgtga ccgacgagct
      661 ggcgaggtga tccgctacga gcttccagac gggcacgtag aggtttccgc agggccggcc
      721 ggcatggcca gtgtgtggga ttacgacctg gtactgatgg cggtttccca tctaaccgaa
      781 tccatgaacc gataccggga agggaaggga gacaagcccg gccgcgtgtt ccgtccacac
      841 gttgcggacg tactcaagtt ctgccggcga gccgatggcg gaaagcagaa agacgacctg
      901 gtagaaacct gcattcggtt aaacaccacg cacgttgcca tgcagcgtac gaagaaggcc
      961 aagaacggcc gcctggtgac ggtatccgag ggtgaagcct tgattagccg ctacaagatc
     1021 gtaaagagcg aaaccgggcg gccggagtac atcgagatcg agctagctga ttggatgtac
     1081 cgcgagatca cagaaggcaa gaacccggac gtgctgacgg ttcaccccga ttactttttg
     1141 atcgatcccg gcatcggccg ttttctctac cgcctggcac gccgcgccgc aggcaaggca
     1201 gaagccagat ggttgttcaa gacgatctac gaacgcagtg gcagcgccgg agagttcaag
     1261 aagttctgtt tcaccgtgcg caagctgatc gggtcaaatg acctgccgga gtacgatttg
     1321 aaggaggagg cggggcaggc tggcccgatc ctagtcatgc gctaccgcaa cctgatcgag
     1381 ggcgaagcat ccgccggttc ctaatgtacg gagcagatgc tagggcaaat tgccctagca
     1441 ggggaaaaag gtcgaaaagg tctctttcct gtggatagca cgtacattgg gaacccaaag
     1501 ccgtacattg ggaaccggaa cccgtacatt gggaacccaa agccgtacat tgggaaccgg
     1561 tcacacatgt aagtgactga tataaaagag aaaaaaggcg atttttccgc ctaaaactct
     1621 ttaaaactta ttaaaactct taaaacccgc ctggcctgtg cataactgtc tggccagcgc
     1681 acagccgaag agctgcaaaa agcgcctacc cttcggtcgc tgcgctccct acgccccgcc
     1741 gcttcgcgtc ggcctatcgc ggccgctggc cgctcaaaaa tggctggcct acggccaggc
     1801 aatctaccag ggcgcggaca agccgcgccg tcgccactcg accgccggcg cccacatcaa
     1861 ggcaccctgc ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc
     1921 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc
     1981 gtcagcgggt gttggcgggt gtcggggcgc agccatgacc cagtcacgta gcgatagcgg
     2041 agtgtatact ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg
     2101 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct
     2161 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     2221 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     2281 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     2341 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     2401 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     2461 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     2521 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     2581 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     2641 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     2701 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     2761 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     2821 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     2881 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     2941 tctacggggt ctgacgctca gtgctgatga atcccctaat gattttggta aaaatcatta
     3001 agttaaggtg gatacacatc ttgtcatatg atcaaatggt ttcgcgaaaa atcaataatc
     3061 agacaacaag atgtgcgaac tcgatatttt acacgactct ctttaccaat tctgccccga
     3121 attacactta aaacgactca acagcttaac gttggcttgc cacgcattac ttgactgtaa
     3181 aactctcact cttaccgaac ttggccgtaa cctgccaacc aaagcgagaa caaaacataa
     3241 catcaaacga atcgaccgat tgttaggtaa tcgtcacctc cacaaagagc gactcgctgt
     3301 ataccgttgg catgctagct ttatctgttc gggcaatacg atgcccattg tacttgttga
     3361 ctggtctgat attcgtgagc aaaaacgact tatggtattg cgagcttcag tcgcactaca
     3421 cggtcgttct gttactcttt atgagaaagc gttcccgctt tcagagcaat gttcaaagaa
     3481 agctcatgac caatttctag ccgaccttgc gagcattcta ccgagtaaca ccacaccgct
     3541 cattgtcagt gatgctggct ttaaagtgcc atggtataaa tccgttgaga agctgggttg
     3601 gtactggtta agtcgagtaa gaggaaaagt acaatatgca gacctaggag cggaaaactg
     3661 gaaacctatc agcaacttac atgatatgtc atctagtcac tcaaagactt taggctataa
     3721 gaggctgact aaaagcaatc caatctcatg ccaaattcta ttgtataaat ctcgctctaa
     3781 aggccgaaaa aatcagcgct cgacacggac tcattgtcac cacccgtcac ctaaaatcta
     3841 ctcagcgtcg gcaaaggagc catgggttct agcaactaac ttacctgttg aaattcgaac
     3901 acccaaacaa cttgttaata tctattcgaa gcgaatgcag attgaagaaa ccttccgaga
     3961 cttgaaaagt cctgcctacg gactaggcct acgccatagc cgaacgagca gctcagagcg
     4021 ttttgatatc atgctgctaa tcgccctgat gcttcaacta acatgttggc ttgcgggcgt
     4081 tcatgctcag aaacaaggtt gggacaagca cttccaggct aacacagtca gaaatcgaaa
     4141 cgtactctca acagttcgct taggcatgga agttttgcgg cattctggct acacaataac
     4201 aagggaagac ttactcgtgg ctgcaaccct actagctcaa aatttattca cacatggtta
     4261 cgctttgggg aaattatgag gggatctctc agcgctcagt ggaacgaaaa ctcacgttaa
     4321 gggattttgg tcatgcattc taggtactaa aacaattcat ccagtaaaat ataatatttt
     4381 attttctccc aatcaggctt gatccccagt aagtcaaaaa atagctcgac atactgttct
     4441 tccccgatat cctccctgat cgaccggacg cagaaggcaa tgtcatacca cttgtccgcc
     4501 ctgccgcttc tcccaagatc aataaagcca cttactttgc catctttcac aaagatgttg
     4561 ctgtctccca ggtcgccgtg ggaaaagaca agttcctctt cgggcttttc cgtctttaaa
     4621 aaatcataca gctcgcgcgg atctttaaat ggagtgtctt cttcccagtt ttcgcaatcc
     4681 acatcggcca gatcgttatt cagtaagtaa tccaattcgg ctaagcggct gtctaagcta
     4741 ttcgtatagg gacaatccga tatgtcgatg gagtgaaaga gcctgatgca ctccgcatac
     4801 agctcgataa tcttttcagg gctttgttca tcttcatact cttccgagca aaggacgcca
     4861 tcggcctcac tcatgagcag attgctccag ccatcatgcc gttcaaagtg caggaccttt
     4921 ggaacaggca gctttccttc cagccatagc atcatgtcct tttcccgttc cacatcatag
     4981 gtggtccctt tataccggct gtccgtcatt tttaaatata ggttttcatt ttctcccacc
     5041 agcttatata ccttagcagg agacattcct tccgtatctt ttacgcagcg gtatttttcg
     5101 atcagttttt tcaattccgg tgatattctc attttagcca tttattattt ccttcctctt
     5161 ttctacagta tttaaagata ccccaagaag ctaattataa caagacgaac tccaattcac
     5221 tgttccttgc attctaaaac cttaaatacc agaaaacagc tttttcaaag ttgttttcaa
     5281 agttggcgta taacatagta tcgacggagc cgattttgaa accgcggtga tcacaggcag
     5341 caacgctctg tcatcgttac aatcaacatg ctaccctccg cgagatcatc cgtgtttcaa
     5401 acccggcagc ttagttgccg ttcttccgaa tagcatcggt aacatgagca aagtctgccg
     5461 ccttacaacg gctctcccgc tgacgccgtc ccggactgat gggctgcctg tatcgagtgg
     5521 tgattttgtg ccgagctgcc ggtcggggag ctgttggctg gctggtggca ggatatattg
     5581 tggtgtaaac aaattgacgc ttagacaact taataacaca ttgcggacgt ttttaatgta
     5641 ctgaattaac gccgaattaa ttcgggggat ctggatttta gtactggatt ttggttttag
     5701 gaattagaaa ttttattgat agaagtattt tacaaataca aatacatact aagggtttct
     5761 tatatgctca acacatgagc gaaaccctat aggaacccta attcccttat ctgggaacta
     5821 ctcacacatt attatggaga aactcgagct tgtcgatcga cagatcccgg tcggcatcta
     5881 ctctatttct ttgccctcgg acgagtgctg gggcgtcggt ttccactatc ggcgagtact
     5941 tctacacagc catcggtcca gacggccgcg cttctgcggg cgatttgtgt acgcccgaca
     6001 gtcccggctc cggatcggac gattgcgtcg catcgaccct gcgcccaagc tgcatcatcg
     6061 aaattgccgt caaccaagct ctgatagagt tggtcaagac caatgcggag catatacgcc
     6121 cggagtcgtg gcgatcctgc aagctccgga tgcctccgct cgaagtagcg cgtctgctgc
     6181 tccatacaag ccaaccacgg cctccagaag aagatgttgg cgacctcgta ttgggaatcc
     6241 ccgaacatcg cctcgctcca gtcaatgacc gctgttatgc ggccattgtc cgtcaggaca
     6301 ttgttggagc cgaaatccgc gtgcacgagg tgccggactt cggggcagtc ctcggcccaa
     6361 agcatcagat catcgagagc ctgcgcgacg gacgcactga cggtgtcgtc catcacagtt
     6421 tgccagtgat acacatgggg atcagcaatc gcgcatatga aatcacgcca tgtagtgtat
     6481 tgaccgattc cttgcggtcc gaatgggccg aacccgctcg tctggctaag atcggccgca
     6541 gcgatcgcat ccatagcctc cgcgaccggt tgtagaacag cgggcagttc ggtttcaggc
     6601 aggtcttgca acgtgacacc ctgtgaacgg cgggagatgc aataggtcag gctctcgcta
     6661 aactccccaa tgtcaagcac ttccggaatc gggagcgcgg ccgatgcaaa gtgccgataa
     6721 acataacgat ctttgtagaa accatcggcg cagctattta cccgcaggac atatccacgc
     6781 cctcctacat cgaagctgaa agcacgagat tcttcgccct ccgagagctg catcaggtcg
     6841 gagacgctgt cgaacttttc gatcagaaac ttctcgacag acgtcgcggt gagttcaggc
     6901 tttttcatat ctcattgccc cccggatctg cgaaagctcg agagagatag atttgtagag
     6961 agagactggt gatttcagcg tgtcctctcc aaatgaaatg aacttcctta tatagaggaa
     7021 gggtcttgcg aaggatagtg ggattgtgcg tcatccctta cgtcagtgga gatatcacat
     7081 caatccactt gctttgaaga cgtggttgga acgtcttctt tttccacgat gctcctcgtg
     7141 ggtgggggtc catctttggg accactgtcg gcagaggcat cttgaacgat agcctttcct
     7201 ttatcgcaat gatggcattt gtaggtgcca ccttcctttt ctactgtcct tttgatgaag
     7261 tgacagatag ctgggcaatg gaatccgagg aggtttcccg atattaccct ttgttgaaaa
     7321 gtctcaatag ccctttggtc ttctgagact gtatctttga tattcttgga gtagacgaga
     7381 gtgtcgtgct ccaccatgtt cacatcaatc cacttgcttt gaagacgtgg ttggaacgtc
     7441 ttctttttcc acgatgctcc tcgtgggtgg gggtccatct ttgggaccac tgtcggcaga
     7501 ggcatcttga acgatagcct ttcctttatc gcaatgatgg catttgtagg tgccaccttc
     7561 cttttctact gtccttttga tgaagtgaca gatagctggg caatggaatc cgaggaggtt
     7621 tcccgatatt accctttgtt gaaaagtctc aatagccctt tggtcttctg agactgtatc
     7681 tttgatattc ttggagtaga cgagagtgtc gtgctccacc atgttggcaa gctgctctag
     7741 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     7801 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
     7861 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
     7921 agcggataac aatttcacac aggaaacagc tatgacatga ttacgaattc ggtccccaga
     7981 ttagcctttt caatttcaga aagaatgcta acccacagat ggttagagag gcttacgcag
     8041 caggtctcat caagacgatc tacccgagca ataatctcca ggaaatcaaa taccttccca
     8101 agaaggttaa agatgcagtc aaaagattca ggactaactg catcaagaac acagagaaag
     8161 atatatttct caagatcaga agtactattc cagtatggac gattcaaggc ttgcttcaca
     8221 aaccaaggca agtaatagag attggagtct ctaaaaaggt agttcccact gaatcaaagg
     8281 ccatggagtc aaagattcaa atagaggacc taacagaact cgccgtaaag actggcgaac
     8341 agttcataca gagtctctta cgactcaatg acaagaagaa aatcttcgtc aacatggtgg
     8401 agcacgacac acttgtctac tccaaaaata tcaaagatac agtctcagaa gaccaaaggg
     8461 caattgagac ttttcaacaa agggtaatat ccggaaacct cctcggattc cattgcccag
     8521 ctatctgtca ctttattgtg aagatagtgg aaaaggaagg tggctcctac aaatgccatc
     8581 attgcgataa aggaaaggcc atcgttgaag atgcctctgc cgacagtggt cccaaagatg
     8641 gacccccacc cacgaggagc atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc
     8701 aagtggattg atgtgatatc tccactgacg taagggatga cgcacaatcc cactatcctt
     8761 cgcaagaccc ttcctctata taaggaagtt catttcattt ggagagaaca cgggggacgt
     8821 cgactccgag gatccggtac ccggtatcga tttaaaaggc ctatggtgag caagggcgag
     8881 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
     8941 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
     9001 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccttcggc
     9061 tacggcctgc agtgcttcgc ccgctacccc gaccacatga agcagcacga cttcttcaag
     9121 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac
     9181 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
     9241 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac
     9301 aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc
     9361 aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac
     9421 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag ctaccagtcc
     9481 gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
     9541 gccgccggga tcactctcgg catggacgag ctgtacaaga ggcctcaggt cgactctcga
     9601 ggatcaacta gtgagctcag tacccggtat cgatttaaag aatttccctg actgcagagc
     9661 tttcgttcgt atcatcggtt tcgacaacgt tcgtcaagtt caatgcatca gtttcattgc
     9721 gcacacacca gaatcctact gagtttgagt attatggcat tgggaaaact gtttttcttg
     9781 taccatttgt tgtgcttgta atttactgtg ttttttattc ggttttcgct atcgaactgt
     9841 gaaatggaaa tggatggaga agagttaatg aatgatatgg tccttttgtt cattctcaaa
     9901 ttaatattat ttgttttttc tcttatttgt tgtgtgttga atttgaaatt ataagagata
     9961 tgcaaacatt ttgttttgag taaaaatgtg tcaaatcgtg gcctctaatg accgaagtta
    10021 atatgaggag taaaacactt gtagttgtac cattatgctt attcactagg caacaaatat
    10081 attttcagac ctagaaaagc tgcaaatgtt actgaataca agtatgtcct cttgtgtttt
    10141 agacatttat gaactttcct ttatgtaatt ttccagaatc cttgtcagat tctaatcatt
    10201 gctttataat tatagttata ctcatggatt tgtagttgag tatgaaaata ttttttaatg
    10261 cattttatga cttgccaatt gattgacaac atgcatcaag cttggcactg gccgtcgttt
    10321 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
    10381 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
    10441 tgcgcagcct gaatggcgaa tgctagagca gcttgagctt ggatcagatt gtcgtttccc
    10501 gccttcagtt taaactatca gtgtttgaca ggatatattg gcgggtaaac ctaagagaaa
    10561 agagcgttta ttagaataat cggatattta aaagggcgtg aaaaggttta tccgttcgtc
    10621 catttgtatg tgcatgccaa ccacagggtt cccctcggga tcaaagtact ttgatccaac
    10681 ccctccgctg ctatagtgca gtcggcttct gacgttcagt gcagccgtct tctgaaaacg
    10741 acatgtcgca caagtcctaa gttacgcgac aggctgccgc cctgcccttt tcctggcgtt
    10801 ttcttgtcgc gtgttttagt cgcataaagt agaatacttg cgactagaac cggagacatt
    10861 acgccatgaa caagagcgcc gccgctggcc tgctgggcta tgcccgcgtc agcaccgacg
    10921 accaggactt gaccaaccaa cgggccgaac tgcacgcggc cggctgcacc aagctgtttt
    10981 ccgagaagat caccggcacc aggcgcgacc gcccggagct ggccaggatg cttgaccacc
    11041 tacgccctgg cgacgttgtg acagtgacca ggctagaccg cctggcccgc agcacccgcg
    11101 acctactgga cattgccgag cgcatccagg aggccggcgc gggcctgcgt agcctggcag
    11161 agccgtgggc cgacaccacc acgccggccg gccgcatggt gttgaccgtg ttcgccggca
    11221 ttgccgagtt cgagcgttcc ctaatcatcg accgcacccg gagcgggcgc gaggccgcca
    11281 aggcccgagg cgtgaagttt ggcccccgcc ctaccctcac cccggcacag atcgcgcacg
    11341 cccgcgagct gatcgaccag gaaggccgca ccgtgaaaga ggcggctgca ctgcttggcg
    11401 tgcatcgctc gaccctgtac cgcgcacttg agcgcagcga ggaagtgacg cccaccgagg
    11461 ccaggcggcg cggtgccttc cgtgaggacg cattgaccga ggccgacgcc ctggcggccg
    11521 ccgagaatga acgccaagag gaacaagcat gaaaccgcac caggacggcc aggacgaacc
    11581 gtttttcatt accgaagaga tcgaggcgga gatgatcgcg gccgggtacg tgttcgagcc
    11641 gcccgcgcac gtctcaaccg tgcggctgca tgaaatcctg gccggtttgt ctgatgccaa
    11701 gctggcggcc tggccggcca gcttggccgc tgaagaaacc gagcgccgcc gtctaaaaag
    11761 gtgatgtgta tttgagtaaa acagcttgcg tcatgcggtc gctgcgtata tgatgcgatg
    11821 agtaaataaa caaatacgca aggggaacgc atgaaggtta tcgctgtact taaccagaaa
    11881 ggcgggtcag gcaagacgac catcgcaacc catctagccc gcgccctgca actcgccggg
    11941 gccgatgttc tgttagtcga ttccgatccc cagggcagtg cccgcgattg ggcggccgtg
    12001 cgggaagatc aaccgctaac cgttgtcggc atcgaccgcc cgacgattga ccgcgacgtg
    12061 aaggccatcg gccggcgcga cttcgtagtg atcgacggag cgccccaggc ggcggacttg
    12121 gctgtgtccg cgatcaaggc agccgacttc gtgctgattc cggtgcagcc aagcccttac
    12181 gacatatggg ccaccgccga cctggtggag ctggttaagc agcgcattga ggtcacggat
    12241 ggaaggctac aagcggcctt tgtcgtgtcg cgggcgatca aaggcacgcg catcggcggt
    12301 gaggttgccg aggcgctggc cgggtacgag ctgcccattc ttgagtcccg tatcacgcag
    12361 cgcgtgagct acccaggcac tgccgccgcc ggcacaaccg ttcttgaatc agaacccgag
    12421 ggcgacgctg cccgcgaggt ccaggcgctg gccgctgaaa ttaaatcaaa actcatttga
    12481 gttaatgagg taaagagaaa atgagcaaaa gcacaaacac gctaagtgcc ggccgtccga
    12541 gcgcacgcag cagcaaggct gcaacgttgg ccagcctg
//