pCytERM-mBiyu-N1

LOCUS       pCytERM-mBiyu-N1      4842 bp DNA     circular SYN 12-MAY-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 4842)
  AUTHORS   ustc
  TITLE     Direct Submission
  JOURNAL   Exported Sep 18, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..4842
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             620..706
                     /codon_start=1
                     /label=p450(29)
                     /translation="MDPVVVLGLCLSCLLLLSLWKQSYGGGKL"
     regulatory      755..757
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             758..1504
                     /codon_start=1
                     /label=mBiyu
                     /translation="MVSKGEEDNMASYGALLFREKIPYVVEMEGDVEGMKFSVRGKGHG
                     DANTGKIEASFICTTGELPVPWSSILTTVTYGAQCFAKYPNDIKDYLKSTMPEGYVQER
                     TITFENDGIYKTRAEVTYEKGSVYNRVTLNGSGFKKGGNILGKKLEFNFNASRTYVLPD
                     VENNGIKCYINIRHNVIGGGQIRAEHQQLNTPLGGGPVDIPHYHAIQTHTILSKDPKET
                     RDHMNVVEVFRAIDCKTAYAGMDELYK"
     polyA_signal    1630..1751
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1758..2213)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        2240..2344
                     /gene="bla"
                     /label=AmpR promoter
     promoter        2346..2703
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2554..2689
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2738..3532
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    3764..3811
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4140..4728
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctccacca tggatcctgt ggtggtgctg gggctctgtc tctcctgttt
      661 gcttctcctt tcactctgga aacagagcta tgggggaggg aagcttcgaa ttctgcagtc
      721 gacggtaccg cgggcccggg atccaccggt cgccaccatg gtgagcaagg gcgaggagga
      781 caacatggcc tcctacggag ccctgctgtt tagagagaag atcccctacg tggtggagat
      841 ggagggcgat gtggagggaa tgaagttctc cgtgagaggc aagggccacg gcgatgctaa
      901 caccggaaag atcgaggcca gctttatctg caccaccggc gagctgcccg tgccttggag
      961 cagcatcctg acaaccgtga catacggcgc ccagtgcttc gctaagtacc ctaatgatat
     1021 caaggactac ctgaagagca ccatgcccga gggctacgtg caggaaagaa ccatcacatt
     1081 tgagaacgat ggcatctaca agaccagggc cgaggtgacc tacgagaagg gaagcgtgta
     1141 caacagggtg acactgaacg gcagcggctt caagaagggc ggaaacatcc tgggcaagaa
     1201 gctggagttc aattttaacg cctccaggac atacgtgctg cctgacgtgg agaacaatgg
     1261 catcaagtgt tacatcaaca tcaggcacaa cgtgatcggc ggcggccaaa tcagggctga
     1321 acaccaacag ctgaataccc ccctgggcgg aggacctgtt gacattccac actaccacgc
     1381 catccagacc cacaccatcc tgtccaagga ccccaaggag acaagggacc acatgaacgt
     1441 ggtggaggtg tttagagcca tcgattgcaa gacagcctac gccggcatgg atgagctgta
     1501 caagtaaagc ggccgcgact ctagatcata atcagccata ccacatttgt agaggtttta
     1561 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt
     1621 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     1681 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     1741 aatgtatctt aaggcgtaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt
     1801 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc
     1861 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
     1921 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
     1981 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg
     2041 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
     2101 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
     2161 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg
     2221 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     2281 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     2341 gagtcctgag gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc
     2401 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
     2461 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     2521 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     2581 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     2641 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     2701 aaagatcgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca
     2761 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
     2821 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
     2881 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc
     2941 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
     3001 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
     3061 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
     3121 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
     3181 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
     3241 cgaactgttc gccaggctca aggcgagcat gcccgacggc gaggatctcg tcgtgaccca
     3301 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
     3361 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
     3421 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
     3481 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact
     3541 ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc
     3601 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     3661 atcctccagc gcggggatct catgctggag ttcttcgccc accctagggg gaggctaact
     3721 gaaacacgga aggagacaat accggaagga acccgcgcta tgacggcaat aaaaagacag
     3781 aataaaacgc acggtgttgg gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg
     3841 cactctgtcg ataccccacc gagaccccat tggggccaat acgcccgcgt ttcttccttt
     3901 tccccacccc accccccaag ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg
     3961 gcaggccctg ccatagcctc aggttactca tatatacttt agattgattt aaaacttcat
     4021 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     4081 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     4141 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     4201 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     4261 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     4321 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     4381 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     4441 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     4501 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     4561 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     4621 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     4681 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     4741 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     4801 ttatcccctg attctgtgga taaccgtatt accgccatgc at
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