| 质粒名称: | pJYS1Peftu |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 85546 |
| 存储实验室: | Sheng Yang |
|
载体骨架基本信息 |
|
| 载体名称: | pXMJ19 |
| 载体出品公司: | -- |
| 载体抗性: | 卡那霉素 |
| 空载体大小: | 5600 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 细菌表达，CRISPR；穿梭载体谷氨酸棒杆菌/大肠杆菌 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Cpf1 |
| 物种来源: | Francisella tularensis subsp. novicida |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | etfu |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pJYS1Peftu was a gift from Sheng Yang (Addgene plasmid # 85546 ; http://n2t.net/addgene:85546 ; RRID:Addgene_85546) |
| 参考文献部分: | CRISPR-Cpf1 assisted genome editing of Corynebacterium glutamicum. Jiang Y, Qian F, Yang J, Liu Y, Dong F, Xu C, Sun B, Chen B, Xu X, Li Y, Wang R, Yang S. Nat Commun. 2017 May 4;8:15179. doi: 10.1038/ncomms15179. 10.1038/ncomms15179 PubMed 28469274 |
买家导航
pJYS1Peftu载体质粒基本信息
pJYS1Peftu质粒图谱载体图谱和pJYS1Peftu载体序列质粒序列多克隆位点信息
pJYS1Peftu质粒载体简介
pJYS1Peftu质粒序列载体序列
LOCUS pJYS1Peftu 10947 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Constitutive expression of FnCpf1 and recT in C.glutamitum, etfu
promoter.
ACCESSION .
VERSION .
KEYWORDS pJYS1Peftu
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10947)
AUTHORS Jiang Y, Qian F, Yang J, Liu Y, Dong F, Xu C, Sun B, Chen B, Xu X,
Li Y, Wang R, Yang S
TITLE CRISPR-Cpf1 assisted genome editing of Corynebacterium glutamicum.
JOURNAL Nat Commun. 2017 May 4;8:15179. doi: 10.1038/ncomms15179.
PUBMED 28469274
REFERENCE 2 (bases 1 to 10947)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..10947
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 3671..4465
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(3725..3744)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 4335..4354
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
terminator 8504..8531
/label=rrnB T2 terminator
/note="transcription terminator T2 from the E. coli rrnB
gene"
terminator 8623..8708
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
CDS complement(9930..10880)
/codon_start=1
/gene="rep101"
/product="RepA protein needed for replication with the
pSC101 origin"
/label=Rep101
/translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
LSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
rep_origin 10928..203
/label=pSC101 ori
/note="low-copy replication origin that requires the Rep101
protein"
ORIGIN
1 ctagtccttt tcctttgagt tgtgggtatc tgtaaattct gctagacctt tgctggaaaa
61 cttgtaaatt ctgctagacc ctctgtaaat tccgctagac ctttgtgtgt tttttttgtt
121 tatattcaag tggttataat ttatagaata aagaaagaat aaaaaaagat aaaaagaata
181 gatcccagcc ctgtgtataa ctcactactt tagtcagttc cgcagtatta caaaaggatg
241 tcgcaaacgc tgtttgctcc tctacaaaac agaccttaaa accctaaagg cttaagtagc
301 accctcgcaa gctcgggcaa atcgctgaat attccttttg tctccgacca tcaggcacct
361 gagtcgctgt ctttttcgtg acattcagtt cgctgcgctc acggctctgg cagtgaatgg
421 gggtaaatgg cactacaggc gccttttatg gattcatgca aggaaactac ccataataca
481 agaaaagccc gtcacgggct tctcagggcg ttttatggcg ggtctgctat gtggtgctat
541 ctgacttttt gctgttcagc agttcctgcc ctctgatttt ccagtctgac cacttcggat
601 tatcccgtga caggtcattc agactggcta atgcacccag taaggcagcg gtatcatcaa
661 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
721 aaaggatctt cacctagatc ctttacgctc cgggacgcca acaacaagac ccatcatagt
781 ttgcccccgc gacattgacc ataaattcat cgcacaaaat atcgaacggg gtttatgccg
841 cttttagtgg gtgcgaagaa tagtctgctc attacccgcg aacaccgccg cattcagatc
901 acgcttagta gcgtccccat gagtaggcag aaccgcgtcc aagtccacat catccataac
961 gatcatgcac ggggtggaat ccacacccag acttgccagc acctcattag cgacacgttg
1021 cgcagcggcc acgtccttag ccttatccac gcaatcgaga acgtactgcc taaccgcgaa
1081 atcagactga atcagtttcc aatcatcggg cttcaccaaa gcaacagcaa cgcgggttga
1141 ttcgacccgt tccggtgctt ccagaccggc gagcttgtac agttcttctt ccatttcacg
1201 acgtacatca gcgtctatgt aatcaatgcc caaagcacgc ttagccccac gtgaccagga
1261 cgaacgcagg tttttagaac caacctcata ctcacgccac cgagccacca aaacagcgtc
1321 catatcctcg ccggcgtcgc tttgatcggc caacatatcc aacatctgaa acggcgtgta
1381 cgacccctta gacgcggttt tagtagcgga gccagtcagt tcctgagaca tgcccttagc
1441 gaggtaggtt gccattttcg cagcgtctcc accccaggta gacacctgat caagtttgac
1501 cccgtgctca cgcagtggcg cgtccatacc ggccttaacc acaccagcag accagcggga
1561 aaacatggaa tcctcaaacg ccttgagttc atcgtcagac agtggacgat ccaagaacaa
1621 cagcatgttg cggtgcaagt gccaaccgtt cgcccaagag tctgtgacct catagtcact
1681 ataggtgtgc tccaccccgt accgtgcacg ttctttcttc cactgagatg ttttcaccat
1741 cgaagagtac gcagtcttaa tacccgcttc aacctgcgca aatgactgtg agcggttgtg
1801 tcgaacagtg cccacaaaca tcatgagcgc gccacccgcc gccaagtgat tcttagtagc
1861 aatagccagc tcaatgcggc gttcgcccat gacttccaat tcagccagag atgaccccca
1921 gcgagagtga gagttttgca gaccctcaaa ctgcgaagca ccgttagacg accaggacac
1981 cgcaacagct tcgtccctgc gccacctatg gcaccccgcc agagccttac tattggtgat
2041 cttgtacatg acgttttgcc tacgccacgc cctagcgcga gtgaccttag aaccctcatt
2101 gacctgcggt tccttagagg tgttcacttc tatttcagtg ttacctagac ccgatgttgt
2161 gcggggttgc gcagtgcgag tttgtgcggg tgttgtgccc gttgtcttag ctagtgctat
2221 ggttgtcaat tgaaacccct tcgggttatg tggcccccgt gcatatgagt tggtagctcg
2281 cacgggggtt tgtcttgtct agggactatt aatttttagt ggtgtttggt ggccgcctag
2341 cttggctatg cgtgccagct tacccgtact caatgttaaa gatttgcatc gacatgggag
2401 ggttacgtgt ccgataccta gggggggtat ccgcgactag gtgccccggt gctcactgtc
2461 tgtaccggcg gggcaagccc cacaccccgc atggacaggg tggctccgcc ccctgcaccc
2521 ccagcaatct gcatgtacat gttttacaca ttagcacgac atgactgcat gtgcatgcac
2581 tgcatgcaga ctaggtaaat atgagtatgt acgactagta acaggagcac tgcacataat
2641 gaatgagttg caggacaatg tttgctacgc atgcgcatga catatcgcag gaaagctact
2701 agagtcttaa agcatggcaa ccaaggcaca gctagaacag caactacaag aagctcaaca
2761 ggcactacag gcgcagcaag cgcaggcaca agccaccatc gaagcactag aagcgcaggc
2821 aaaggctaag cccgtcgtgg tcaccgcacg cgttcctttg gcactacgtg aggacatgaa
2881 gcgcgcaggc atgcagaacg gtgaaaacct ccaagagttc atgatcgccg cgtttaccga
2941 gcggctagaa aagctcacca ccaccgacaa cgaggaaaac aatgtctaac ccactagttc
3001 tctttgccca ccgtgacccg gtaaatgacg tgacgttcga gtgcattgag cacgccacct
3061 acgacacact ttcacacgct aaagaccaga tcaccgccca aatgcaagcc ctagacgaag
3121 aagccgccct actgccctaa tgggtgtttc atgggtgttt ccctagtgtt tcatggtgtt
3181 ttcacctaag ctagggaatt gcgcgagaag tctcgcaaaa atcagcaacc cccggaacca
3241 cacagttcac gggggttctt ctatgccaga aatcagaaag gggaaccagt gaacgacccc
3301 gaatggctgg atgatcctcc ttgggccacg cccacccggc gatcatcgag gcggtcaccg
3361 gaagcggaac acgtagaaag ccagtccgca gaaacggtgc tgaccccgga tgaatgtcag
3421 ctactgggct atctggacaa gggaaaacgc aagcgcaaag agaaagcagg tagcttgcag
3481 tgggcttaca tggcgatagc tagactgggc ggttttatgg acagcaagcg aaccggaatt
3541 gccagctggg gcgccctctg gtaaggttgg gaagccctgc aaagtaaact ggatggcttt
3601 cttgccgcca aggatctgat ggcgcagggg atcaagatct gatcaagaga caggatgagg
3661 atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga
3721 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt
3781 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct
3841 gaatgaactc caagacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg
3901 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt
3961 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc
4021 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc
4081 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga
4141 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg
4201 gatgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat
4261 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg
4321 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc
4381 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta
4441 tcgccttctt gacgagttct tctgagcggg actctggggt tcgcggaatc atgacctgag
4501 ctgtttacaa ttaatcatcg tgtggtacca tgtgtggaat tggaaaggac ttgaacgatg
4561 tccatctacc aagagtttgt gaataaatac tccctgtcca agaccctccg ttttgagctg
4621 atcccccaag gcaagaccct cgaaaacatc aaggcacgcg gcctcatcct ggatgacgaa
4681 aagcgcgcta aggattacaa gaaggcaaag cagatcatcg acaagtacca ccagttcttc
4741 atcgaagaga tcctgtcctc cgtgtgcatc tccgaggacc tgctccagaa ctactccgat
4801 gtctacttca agctcaagaa gtccgatgac gataacctgc agaaggactt caagtccgct
4861 aaggatacca tcaagaagca gatctccgaa tacatcaagg attccgagaa gttcaagaac
4921 ctcttcaacc agaacctgat cgacgcaaag aagggccagg aatccgatct catcctgtgg
4981 ctcaagcagt ccaaggataa cggcatcgag ctcttcaagg ccaactccga catcaccgac
5041 atcgatgaag ctctggagat catcaagtcc ttcaagggct ggaccaccta cttcaagggc
5101 ttccacgaaa accgcaagaa cgtgtactcc tccaacgata tcccaacctc tatcatctac
5161 cgcatcgtcg acgataacct gccaaagttc ctcgaaaaca aggcaaagta cgagtccctg
5221 aaggataagg ccccagaagc tatcaactac gagcagatca agaaggacct ggccgaagag
5281 ctcaccttcg acatcgatta caagacctct gaagtgaacc agcgcgtctt ctccctcgat
5341 gaagtgttcg agatcgccaa cttcaacaac tacctgaacc agtccggcat caccaagttc
5401 aacaccatca tcggcggcaa gttcgtcaac ggcgaaaaca ccaagcgcaa gggcatcaac
5461 gagtacatca acctctactc ccagcagatc aacgataaga ccctgaagaa gtacaagatg
5521 tccgtgctct tcaagcagat cctgtccgac accgaatcca agtccttcgt catcgacaag
5581 ctggaggacg attccgatgt ggtcaccacc atgcagtcct tctacgaaca gatcgcagcc
5641 ttcaagaccg tggaagagaa gtccatcaag gagaccctct ccctgctctt cgacgatctg
5701 aaggctcaga agctggatct ctccaagatc tacttcaaga acgacaagtc cctgaccgat
5761 ctctcccagc aggtcttcga cgattactcc gtgatcggca ccgcagtcct ggaatacatc
5821 acccagcaga tcgccccaaa gaacctcgat aacccatcca agaaggaaca ggagctgatc
5881 gccaagaaga ccgaaaaggc taagtacctg tccctcgaga ccatcaagct ggctctcgaa
5941 gagttcaaca agcaccgcga catcgataag cagtgccgct tcgaagagat cctcgcaaac
6001 ttcgctgcaa tcccaatgat cttcgacgaa atcgcacaga acaaggataa cctggcccag
6061 atctccatca agtaccagaa ccagggcaag aaggatctgc tccaggcctc cgctgaggac
6121 gatgtgaagg caatcaagga cctgctcgat cagaccaaca acctgctcca caagctgaag
6181 atcttccaca tctcccagtc cgaagacaag gccaacatcc tcgacaagga tgagcacttc
6241 tacctggtgt tcgaagagtg ctacttcgaa ctcgctaaca tcgtcccact gtacaacaag
6301 atccgcaact acatcaccca gaagccatac tccgatgaaa agttcaagct caacttcgag
6361 aactccaccc tggcaaacgg ctgggacaag aacaaggaac cagataacac cgccatcctc
6421 ttcatcaagg acgataagta ctacctgggc gtgatgaaca agaagaacaa caagatcttc
6481 gacgataagg ccatcaagga aaacaagggc gagggctaca agaagatcgt gtacaagctg
6541 ctcccaggcg ctaacaagat gctcccaaag gtcttcttct ccgcaaagtc catcaagttc
6601 tacaacccat ccgaagatat cctgcgcatc cgcaaccact ccacccacac caagaacggc
6661 tccccacaga agggctacga aaagttcgag ttcaacatcg aagactgccg caagttcatc
6721 gatttctaca agcagtccat ctccaagcac ccagagtgga aggacttcgg cttccgcttc
6781 tccgataccc agcgctacaa ctccatcgat gaattctacc gcgaagtgga gaaccagggc
6841 tacaagctga ccttcgaaaa catctccgag tcctacatcg attccgtggt caaccagggc
6901 aagctgtacc tcttccagat ctacaacaag gacttctccg cttactccaa gggccgccca
6961 aacctgcaca ccctctactg gaaggcactc ttcgacgaac gcaacctgca ggatgtggtc
7021 tacaagctca acggcgaagc agagctgttc taccgcaagc agtccatccc aaagaagatc
7081 acccacccag ccaaggaagc aatcgccaac aagaacaagg ataacccaaa gaaggaatcc
7141 gtgttcgagt acgacctgat caaggataag cgcttcaccg aggacaagtt cttcttccac
7201 tgcccaatca ccatcaactt caagtcctcc ggcgccaaca agttcaacga tgaaatcaac
7261 ctgctcctga aggagaaggc taacgacgtg cacatcctgt ccatcgatcg cggcgaacgc
7321 cacctcgcct actacaccct ggtcgacggc aagggcaaca tcatcaagca ggacaccttc
7381 aacatcatcg gcaacgatcg catgaagacc aactaccacg acaagctggc cgctatcgag
7441 aaggaccgcg attccgctcg caaggattgg aagaagatca acaacatcaa ggaaatgaag
7501 gaaggctacc tctcccaggt ggtccacgaa atcgctaagc tggtgatcga gtacaacgca
7561 atcgtggtct tcgaagacct gaacttcggc ttcaagcgcg gccgcttcaa ggtggagaag
7621 caggtctacc agaagctgga aaagatgctc atcgagaagc tgaactacct cgtgttcaag
7681 gacaacgaat tcgataagac cggcggcgtc ctccgtgcat accagctgac cgccccattc
7741 gagaccttca agaagatggg caagcagacc ggcatcatct actacgtgcc agctggcttc
7801 acctctaaga tctgcccagt gaccggcttc gtcaaccagc tctacccaaa gtacgaatcc
7861 gtctccaagt cccaggagtt cttctccaag ttcgacaaga tctgctacaa cctggataag
7921 ggctacttcg aattctcctt cgactacaag aacttcggcg ataaggcagc caagggcaag
7981 tggaccatcg catccttcgg ctcccgcctc atcaacttcc gcaactccga caagaaccac
8041 aactgggata cccgcgaagt gtacccaacc aaggaactgg agaagctcct gaaggattac
8101 tccatcgaat acggccacgg cgagtgcatc aaggctgcaa tctgcggcga atccgacaag
8161 aagttcttcg caaagctgac ctctgtgctc aacaccatcc tgcagatgcg caactccaag
8221 accggcaccg agctggatta cctcatctcc ccagtggccg acgtcaacgg caacttcttc
8281 gattcccgcc aggctccaaa gaacatgcca caggacgctg atgcaaacgg cgcctaccac
8341 atcggtctga agggtctcat gctcctgggt cgcatcaaga acaaccagga aggcaagaag
8401 ctgaatctcg tcattaagaa cgaagaatac tttgaatttg tccagaaccg caataactaa
8461 gtcgacctgc aggcatgcaa gcttaagagt ttgtagaaac gcaaaaaggc catccgtcag
8521 gatggccttc tgcttaattt gatgcctggc agtttatggc gggcgtcctg cccgccaccc
8581 tccgggccgt tgcttcgcaa cgttcaaatc cgctcccggc ggatttgtcc tactcaggag
8641 agcgttcacc gacaaacaac agataaaacg aaaggcccag tctttcgact gagcctttcg
8701 ttttatttaa attctagatc agtaggcgcg tagggtaagt ggggtagcgg cttgttagat
8761 atcttgaaat cggctttcaa cagcattgat ttcgatgtat ttagctggcc gttaccctgc
8821 gaatgtccac agggtagctg gtagtttgaa aatcaacgcc gttgccctta ggattcagta
8881 actggcacat tttgtaatgc gctagatctg tgtgctcagt cttccaggct gcttatcaca
8941 gtgaaagcaa aaccaattcg tggctgcgaa agtcgtagcc accacgaagt ccaggaggac
9001 atacaatgac taagcaacca ccaatcgcaa aagccgatct gcaaaaaact cagggaaacc
9061 gtgcaccagc agcagttaaa aatagcgacg tgattagttt tattaaccag ccatcaatga
9121 aagagcaact ggcagcagct cttccacgcc atatgacggc tgaacgtatg atccgtatcg
9181 ccaccacaga aattcgtaaa gttccggcgt taggaaactg tgacactatg agttttgtca
9241 gtgcgatcgt acagtgttca cagctcggac ttgagccagg tagcgccctc ggtcatgcat
9301 atttactgcc ttttggtaat aaaaacgaaa agagcggtaa aaagaacgtt cagctaatca
9361 ttggctatcg cggcatgatt gatctggctc gccgttctgg tcaaatcgcc agcctgtcag
9421 cccgtgttgt ccgtgaaggt gacgagttta gcttcgaatt tggccttgat gaaaagttaa
9481 tacaccgccc gggagaaaac gaagatgccc cggttaccca cgtctatgct gtcgcaagac
9541 tgaaagacgg aggtactcag tttgaagtta tgacgcgcaa acagattgag ctggtgcgca
9601 gcctgagtaa agctggtaat aacgggccgt gggtaactca ctgggaagaa atggcaaaga
9661 aaacggctat tcgtcgcctg ttcaaatatt tgcccgtatc aattgagatc cagcgtgcag
9721 tatcaatgga tgaaaaggaa ccactgacaa tcgatcctgc agattcctct gtattaaccg
9781 gggaatacag tgtaatcgat aattcagagg aataattcag cctggcggtg taatgcaccg
9841 ccaacttgaa agttaacagg tgaacagttg ttctactttt gtttgttagt cttgatgctt
9901 cactgataga tacaagagcc ataagaacct cagatccttc cgtatttagc cagtatgttc
9961 tctagtgtgg ttcgttgttt ttgcgtgagc catgagaacg aaccattgag atcatgctta
10021 ctttgcatgt cactcaaaaa ttttgcctca aaactggtga gctgaatttt tgcagttaaa
10081 gcatcgtgta gtgtttttct tagtccgtta tgtaggtagg aatctgatgt aatggttgtt
10141 ggtattttgt caccattcat ttttatctgg ttgttctcaa gttcggttac gagatccatt
10201 tgtctatcta gttcaacttg gaaaatcaac gtatcagtcg ggcggcctcg cttatcaacc
10261 accaatttca tattgctgta agtgtttaaa tctttactta ttggtttcaa aacccattgg
10321 ttaagccttt taaactcatg gtagttattt tcaagcatta acatgaactt aaattcatca
10381 aggctaatct ctatatttgc cttgtgagtt ttcttttgtg ttagttcttt taataaccac
10441 tcataaatcc tcatagagta tttgttttca aaagacttaa catgttccag attatatttt
10501 atgaattttt ttaactggaa aagataaggc aatatctctt cactaaaaac taattctaat
10561 ttttcgcttg agaacttggc atagtttgtc cactggaaaa tctcaaagcc tttaaccaaa
10621 ggattcctga tttccacagt tctcgtcatc agctctctgg ttgctttagc taatacacca
10681 taagcatttt ccctactgat gttcatcatc tgagcgtatt ggttataagt gaacgatacc
10741 gtccgttctt tccttgtagg gttttcaatc gtggggttga gtagtgccac acagcataaa
10801 attagcttgg tttcatgctc cgttaagtca tagcgactaa tcgctagttc atttgctttg
10861 aaaacaacta attcagacat acatctcaat tggtctaggt gattttaatc actataccaa
10921 ttgagatggg ctagtcaatg ataatta
//
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pJYS1Peftu质粒载体应用举例
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