| 质粒名称: | pSL1242 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002428 |
| 产品用途: | Mammalian PhCMV-driven constitutive LOV2(2)-L7Ae expression vector (PhCMV-LOV2(2)-L7Ae-pA). |
| 存储实验室: | Mingqi XIE Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3.1 |
| 表达系统: | 哺乳动物表达 |
| 启动子: | PhCMV |
| 拷贝数: | 未拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | LOV2(2)-L7Ae |
| 物种来源: | synthetic |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Rationally designed light-inducible RNA-releasing protein for translational regulation and optogenetic control of gene therapies
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NA
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pSL1242
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pSL1242载体质粒基本信息
pSL1242质粒图谱载体图谱和pSL1242载体序列质粒序列多克隆位点信息
pSL1242质粒载体简介
pSL1242质粒序列载体序列
LOCUS pSL1242 6132 bp DNA circular SYN 05-MAR-2026
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6132)
AUTHORS Trial User
TITLE Direct Submission
JOURNAL Exported Aug 28, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6132
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 232..819
/label=CMV
primer_bind 863..882
/label=T7
primer_bind 881..929
/label=oSCL2253
misc_feature 909..1299
/label=LOV2
primer_bind complement(1279..1322)
/label=oSCL2254
primer_bind complement(1281..1299)
/label=oSCL1758
primer_bind complement(1281..1299)
/label=oSCL1994
misc_feature 1303..1629
/label=L7Ae
misc_feature 1303..1629
/direction=RIGHT
/note="note=""Fragment extracted from PCR(pWH132.xdna) [363
nt] : (BamHI[6] / XbaI[373])"
primer_bind 1303..1322
/label=oSCL2255
primer_bind complement(1605..1629)
/label=DDL7AeR
/note="EcoRI"
primer_bind complement(1609..1638)
/label=L7Ae RLE
primer_bind complement(1611..1652)
/label=oSCL2256
primer_bind complement(1703..1721)
/label=oYW306
terminator 1729..1956
/label=bGH PA
polyA_signal 1732..1956
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 2002..2430
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2444..2773
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 2624..2759
/label=SV40 ori
/note="SV40 origin of replication"
misc_feature 2624..2701
/label=SV40 ORI
CDS 2840..3634
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 3808..3929
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(3974..3994)
/label=M13-rev
primer_bind complement(3978..3994)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
misc_binding complement(4000..4022)
/label=LacO
protein_bind 4002..4018
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(4026..4056)
/label=lac promoter
/note="promoter for the E. coli lac operon"
promoter complement(4027..4056)
/label=lac
protein_bind 4071..4092
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(4380..4965)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(5136..5996)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(5997..6101)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 gccaccatga attttgtcat cacggacccg cgtctgcccg acaatccgat tatcttcgcg
961 tcagactctt tcttacaact gactgagtat agtagagagg agatattggg gcgtaactgt
1021 agatttcttc aggggccaga aactgatcgg gctaccgttc gcaagatacg tgacgcaata
1081 gacaaccaga ccgaggtgac ggtgcagctg attaactaca caaagtctgg gaagaagttc
1141 tggaacctgt ttcatttaca acctatgagg gaccaaaaag gtgacgttca atatttcatc
1201 ggggttcagt tagatgggac tgagcacgtg agagatgcag cagaaagaga gggtgtaatg
1261 cttattaaaa aaacagccga gaatatcgac gaagccgcta agatgcagaa cgaggccctg
1321 agcctgctgg aaaaagtgcg cgagagcggc aaagtgaaga agggcaccaa cgaaaccacc
1381 aaggccgtgg aacggggcct ggccaagctg gtgtatatcg ccgaggacgt ggaccccccc
1441 gagattgtgg cccatctgcc cctgctgtgc gaagagaaga acgtgcccta catctacgtg
1501 aagtccaaga acgacctggg cagagccgtg ggcatcgagg tgccatgtgc ctctgccgcc
1561 atcatcaacg agggcgagct gcggaaagaa ctgggcagcc tggtggaaaa gatcaagggc
1621 ctgcagaagt aaggatccac tagtccagtg tggtggaatt ctgcagatat ccagcacagt
1681 ggcggccgct cgagtctaga gggcccgttt aaacccgctg atcagcctcg actgtgcctt
1741 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
1801 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
1861 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca
1921 atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct
1981 ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
2041 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
2101 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
2161 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
2221 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
2281 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
2341 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
2401 agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg
2461 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
2521 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
2581 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
2641 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
2701 cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
2761 aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcaagagac
2821 aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc
2881 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc
2941 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc
3001 cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg
3061 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt
3121 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc
3181 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga
3241 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga
3301 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct
3361 caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc
3421 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt
3481 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg
3541 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat
3601 cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc
3661 gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa
3721 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat
3781 ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa
3841 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
3901 ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag
3961 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
4021 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
4081 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
4141 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
4201 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
4261 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
4321 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
4381 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
4441 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
4501 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
4561 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
4621 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
4681 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
4741 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
4801 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
4861 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtttt
4921 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
4981 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
5041 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
5101 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
5161 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
5221 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
5281 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
5341 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
5401 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
5461 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
5521 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
5581 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
5641 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
5701 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
5761 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
5821 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
5881 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
5941 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
6001 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
6061 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
6121 ccacctgacg tc
//
