| 质粒名称: | pAAVLINK-LAMA1-B |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001744 |
| 产品用途: | Body' AAVLINK plasmid for LAMA1 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | Promoterless |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | LAMA1 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-LAMA1-B
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-LAMA1-B载体质粒基本信息
pAAVLINK-LAMA1-B质粒图谱载体图谱和pAAVLINK-LAMA1-B载体序列质粒序列多克隆位点信息
pAAVLINK-LAMA1-B质粒载体简介
pAAVLINK-LAMA1-B质粒序列载体序列
LOCUS pAAVLINK-LAMA1-B 6676 bp DNA circular SYN 22-JUL-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6676)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 5, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6676
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..141
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..141
/label=AAV2 ITR
protein_bind 156..189
/label=loxJTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 190..281
/label=Splicing Acceptor
/note="Splicing acceptor"
misc_feature 282..3832
/label=LAMA1
misc_feature 1056..1061
/label=SDS
misc_feature 1335..1340
/label=SDS
intron 3833..3873
/label=Splicing Donor
misc_feature 3877..3910
/label=lox2272-JT1571
repeat_region 3939..4079
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3939..4068
/label=AAV2 ITR
rep_origin 4154..4609
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4891..4995
/gene="bla"
/label=AmpR promoter
CDS 4996..5856
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6027..6615
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
121 actccatcac taggggttcc tgcggccgca cgcgtataac ttcgtatagc atacattata
181 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
241 tattggtctt actgacatcc actttgcctt tctctccaca gggtctctgc ggctgtgtgg
301 aggaaaccgg ggcctgccct tgcaaggaaa atgtctttgg tcctcagtgc aacgaatgtc
361 gagagggcac cttcgctctc cgcgcagaca accccctggg ctgcagcccg tgcttctgct
421 ccgggctgtc ccacctctgc tcagagctgg aggactacgt gaggacccca gtaacgctgg
481 gctccgatca gcctcttctg cgtgtggttt ctcagagtaa cttgaggggc acgaccgagg
541 gggtttacta ccaggccccc gacttcctgc tggatgccgc caccgtccgg cagcacatcc
601 gtgcagagcc gttttactgg cggctgccgc agcagttcca aggagaccag ctcatggcct
661 atggtggcaa actgaagtac agcgtggcct tctattcttt ggatggcgtc ggcacctcca
721 attttgagcc tcaagttctc atcaaaggtg gtcggatcag aaagcaagtc atttacatgg
781 atgcaccagc cccagagaat ggagtgagac aggaacaaga agtagcaatg agagagaatt
841 tttggaaata ttttaactct gtttctgaaa aacctgtcac gcgagaggat tttatgtctg
901 tcctcagcga tattgagtac atcctcatca aggcatcgta tggtcaagga ttacagcaga
961 gcagaatctc agacatttca atggaggttg gcagaaaggc tgaaaagctg cacccagaag
1021 aagaggttgc atctctttta gagaattgtg tctgtcctcc tggcactgtg ggattctcat
1081 gtcaggactg cgcccctggg taccacagag ggaagctccc agcagggagt gacaggggac
1141 cacgccctct ggttgctcct tgtgttccct gcagttgcaa caaccacagt gacacctgtg
1201 accccaacac cgggaagtgt ctgaactgtg gcgataacac agcaggtgac cattgtgatg
1261 tgtgtacttc tggctactac gggaaggtga ctggctcagc aagtgactgt gctctgtgtg
1321 cctgtcctca cagccctcct gccagtttta gtcccacttg tgtcttggaa ggggaccacg
1381 atttccgttg tgacgcctgt ctcctgggct atgaaggaaa acactgtgaa aggtgctcct
1441 caagctatta tgggaaccct caaacaccag gtggcagttg ccagaagtgt gactgcaacc
1501 cgcacggctc tgtccacggt gactgtgacc gcacatctgg gcagtgcgtt tgcaggctgg
1561 gggcctcggg gctccggtgc gatgagtgtg aaccgaggca cattctgatg gaaacagatt
1621 gtgtttcctg tgatgatgag tgtgtaggtg tgctgctgaa tgacttggat gagattggtg
1681 atgccgttct ttctctgaac ctcactggca ttatccctgt cccatatgga attttgtcaa
1741 acctggaaaa tacaactaaa tatctccagg aatctttatt aaaagaaaat atgcaaaagg
1801 acctgggaaa aattaagctt gaaggtgttg cagaagaaac ggacaacctg caaaagaagc
1861 tcactaggat gttagcgagt acccaaaagg tgaatagggc aactgagaga atcttcaagg
1921 agagtcaaga cctggccata gccattgaga ggctgcagat gagcatcaca gaaattatgg
1981 aaaagacaac tttaaatcag actttggatg aagatttcct actacccaat tctactcttc
2041 agaacatgca acagaatggt acatctttgc tagaaatcat gcagataaga gacttcacac
2101 agttgcacca aaatgccacc cttgaactca aggctgctga agatttattg tcacaaattc
2161 aggaaaatta ccagaagccg ctggaagaat tggaggtatt gaaagaagca gcaagccacg
2221 tcctttcaaa gcacaacaat gaactaaagg cggctgaggc gctcgtgagg gaagctgagg
2281 caaagatgca ggaaagcaac cacctgctgc tcatggtcaa tgctaatctg agagaattca
2341 gtgataaaaa gctgcatgtt caagaagaac aaaatctgac ctcagagctc attgtccaag
2401 gaagaggatt gatagatgct gctgctgcac aaacagatgc tgtacaagat gctctagagc
2461 acttagagga tcaccaggat aagctacttt tatggtctgc caaaatcagg caccacatag
2521 atgacctggt catgcacatg tcccaaagga acgcagtcga cctggtctac agagctgagg
2581 accatgccgc tgagttccag agactagcag atgttctgta cagtggcctt gaaaacatca
2641 gaaatgtgtc cctgaatgcc accagtgcag cctatgtcca ttacaacatc cagagcctga
2701 ttgaagaatc ggaggaactg gccagagatg ctcacaggac tgtgactgag acgagcctgc
2761 tctcagaatc ccttgtttct aacgggaaag cggccgtgca gcgcagctcc agatttctaa
2821 aagaaggcaa caacctcagc aggaagcttc caggtattgc attggaactg agtgaattga
2881 gaaataagac aaacagattt caagagaatg ctgttgaaat taccaggcaa accaatgaat
2941 cactcttgat acttagagca attcctaaag gtataagaga caagggagcc aaaaccaaag
3001 agctggccac gtctgcaagc cagagcgcgg tgagcacgct gagggacgtg gcggggctga
3061 gccaggagct gctgaacaca tctgccagcc tgtccagggt caacaccaca ttacgagaga
3121 cacaccagct tctgcaggac tccaccatgg ccactctgtt ggctggaaga aaagtcaaag
3181 acgtggaaat tcaagccaac cttttgtttg atcggttgaa gcctttgaag atgttagagg
3241 agaatctgag cagaaaccta tcagaaatta aactgttgat cagccaggcc cgcaaacaag
3301 cagcttctat taaagtcgcc gtgtctgcag acagagattg catccgggcc taccagcctc
3361 agatttcctc taccaactac aataccttaa cactaaatgt taagacacag gaacccgata
3421 atcttctctt ctacctcggt agcagcaccg cttctgattt ccttgcagtg gagatgcggc
3481 gagggagagt ggccttcctg tgggacctgg gctccgggtc cacacgcttg gagtttccag
3541 actttcccat tgatgacaac agatggcaca gtatccatgt agccagattt ggaaacattg
3601 gttcactgag tgtaaaggaa atgagctcaa atcaaaagtc accaacaaaa acaagtaaat
3661 cccctgggac agctaatgtt ctggatgtaa acaattcaac actcatgttt gttggaggtc
3721 ttggaggaca aatcaagaaa tctcctgctg tgaaggttac tcattttaaa ggctgcttgg
3781 gggaggcctt cctgaatgga aaatccatag gcctatggaa ctatattgaa aggtaagtat
3841 caaggttaca agacaggttt aaggagacca ataagctatt attcgtatag gatactttat
3901 acgaagttat gaattccacg tgcggaccga gcggccgcag gaacccctag tgatggagtt
3961 ggccactccc tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg
4021 acgcccgggc tttgcccggg cggcctcagt gagcgagcga gcgcgcagct gcctgcaggg
4081 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatacgtca
4141 aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
4201 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
4261 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
4321 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgattt gggtgatggt
4381 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
4441 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcgggctat
4501 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
4561 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt atggtgcact
4621 ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc
4681 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
4741 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga
4801 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
4861 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
4921 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
4981 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
5041 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
5101 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
5161 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
5221 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
5281 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
5341 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
5401 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
5461 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
5521 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
5581 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
5641 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
5701 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
5761 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
5821 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
5881 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
5941 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
6001 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
6061 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
6121 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
6181 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
6241 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
6301 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
6361 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
6421 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
6481 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
6541 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
6601 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
6661 ccttttgctc acatgt
//
