| 质粒名称: | Xlone-mT2(T203G)-Gem1(1-110)-IRES-Cdt1(1-100)(Cy-)-mT2(T62C)-IRES-H1.0-mT2(V68H) |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001815 |
| 产品用途: | For constructing a tr-Fucci(CA)-cyan+H1.0 stably transfected cell line in mammalian cells. |
| 存储实验室: | Xin Zhang Lab |
|
载体骨架基本信息 |
|
| 载体名称: | Xlone |
| 表达系统: | 哺乳动物表达 |
| 启动子: | TRE3GS |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | mT2(T203G)-Gem1(1-110), Cdt1(1-100)(Cy-)-mT2(T62C), H1.0-mT2(V68H) |
| 物种来源: |
synthetic
|
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Time-resolved fluorescent proteins expand fluorescent microscopy in temporal and spectral domains.
Tan Z., Hsiung C-H., Feng J., Zhang Y., Wan Y., Chen J., Sun K., Lu P., Zang J., Yang W., Gao Y., Yin J., Zhu T., Lu Y., Pan Z., Zou Y., Liao C., Li X., Ye Y., Liu Y., Zhang X.
Cell 0, (2025)
|
Xlone-mT2(T203G)-Gem1(1-110)-IRES-Cdt1(1-100)(Cy-)-mT2(T62C)
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
Xlone-mT2(T203G)-Gem1(1-110)-IRES-Cdt1(1-100)(Cy-)-mT2(T62C)载体质粒基本信息
Xlone-mT2(T203G)-Gem1(1-110)-IRES-Cdt1(1-100)(Cy-)-mT2(T62C)质粒图谱载体图谱和Xlone-mT2(T203G)-Gem1(1-110)-IRES-Cdt1(1-100)(Cy-)-mT2(T62C)载体序列质粒序列多克隆位点信息
Xlone-mT2(T203G)-Gem1(1-110)-IRES-Cdt1(1-100)(Cy-)-mT2(T62C)质粒载体简介
Xlone-mT2(T203G)-Gem1(1-110)-IRES-Cdt1(1-100)(Cy-)-mT2(T62C)质粒序列载体序列
LOCUS Xlone-mT2(T203G)-Gem1(1-110)-IRES-Cdt1(1-100)(Cy-)-mT2(T62C)-IRES-H1.0-mT2(V68H) 10364 bp DNA circular SYN 28-JUL-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10364)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Aug 13, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..10364
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 9..376
/label=TRE3GS promoter
/note="3rd-generation Tet-responsive promoter that can be
activated by binding of Tet-On(R) 3G, modified to eliminate
binding sites for endogenous mammalian transcription
factors"
protein_bind 17..35
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 53..71
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 89..107
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 125..143
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 161..179
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 197..215
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 233..251
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
regulatory 403..412
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 409..1125
/codon_start=1
/product="enhanced monomeric variant of CFP (Goedhart et
al., 2012)"
/label=mTurquoise2
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSGQSKLSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
CDS 409..1125
/codon_start=1
/product="enhanced monomeric variant of CFP (Goedhart et
al., 2010)"
/label=mTurquoise
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSGQSKLSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
misc_feature 803..808
/label=SDS
CDS 1156..1485
/codon_start=1
/product="degron consisting of residues 1-110 of human
Geminin (Zielke and Edgar, 2015)"
/label=Gem1 (1-110)
/translation="MNPSMKQKQEEIKENIKNSSVPRRTLKMIQPSASGSLVGRENELS
AGLSKRKHRNDHLTSTTSSPGVIVPESSENKNLGGVTQESFDLMIKENPSSQYWKEVAE
KRRKAL"
misc_feature 3008..3013
/label=SDS
misc_feature 3191..3777
/label=IRES2
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
misc_feature 5227..5232
/label=SDS
polyA_signal 5326..5460
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(5781..5797)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 6271..6375
/gene="bla"
/label=AmpR promoter
CDS 6376..7236
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
misc_feature 7237..8177
/label=ColE1
/note="ColE1"
rep_origin 7407..7995
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
protein_bind 8283..8304
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 8319..8349
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 8357..8373
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 8381..8397
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
polyA_signal 8661..8742
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
CDS complement(8859..9257)
/codon_start=1
/gene="Aspergillus terreus BSD"
/product="blasticidin S deaminase"
/label=BSD
/note="confers resistance to blasticidin"
/translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
KAIVKDSDGQPTAVGIRELLPSGYVWEG"
CDS complement(9258..9314)
/codon_start=1
/product="2A peptide from porcine teschovirus-1
polyprotein"
/label=P2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="ATNFSLLKQAGDVEENPGP"
CDS complement(9324..10067)
/codon_start=1
/product="modified rtTA protein that binds tightly to
promoters containing the tet operator in the presence of
doxycycline"
/label=Tet-On(R) 3G
/translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
DALDDFDLDMLPADALDDFDLDMLPG"
CDS complement(10068..10091)
/codon_start=1
/product="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
/label=FLAG
/translation="DYKDDDDK"
regulatory 10091..10100
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
promoter complement(10120..10331)
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
ORIGIN
1 atcacctcga gtttactccc tatcagtgat agagaacgta tgaagagttt actccctatc
61 agtgatagag aacgtatgca gactttactc cctatcagtg atagagaacg tataaggagt
121 ttactcccta tcagtgatag agaacgtatg accagtttac tccctatcag tgatagagaa
181 cgtatctaca gtttactccc tatcagtgat agagaacgta tatccagttt actccctatc
241 agtgatagag aacgtataag ctttgcttat gtaaaccagg gcgcctataa aagagtgctg
301 attttttgag taaacttcaa ttccacaaca cttttgtctt ataccaactt tccgtaccac
361 ttcctaccct cgtaaaggta ccttggtacc gagctcggat ccgccaccat ggtgagcaag
421 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
481 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
541 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
601 ctgtcctggg gcgtgcagtg cttcgcccgc taccccgacc acatgaagca gcacgacttc
661 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
721 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
781 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
841 aactacttca gcgacaacgt ctatatcacc gccgacaagc agaagaacgg catcaaggcc
901 aacttcaaga tccgccacaa catcgaggac ggcggcgtgc agctcgccga ccactaccag
961 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcggt
1021 cagtccaagc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
1081 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagggcta ccccagccac
1141 tggcggcctc tggaaatgaa ccccagcatg aagcagaagc aggaagagat caaagagaac
1201 atcaagaaca gcagcgtgcc caggcggacc ctgaagatga tccagcccag cgccagcgga
1261 tctctggtgg gccgggagaa tgagctgtct gccggcctga gcaagcggaa gcaccggaac
1321 gaccacctga ccagcaccac ctctagccca ggcgtgatcg tgcccgagag cagcgagaac
1381 aagaacctgg gcggcgtgac ccaagaaagc ttcgacctga tgatcaaaga aaaccccagc
1441 agccagtact ggaaagaggt ggccgagaag cggcggaagg ccctgtaagg taccgagctc
1501 ggatcctact ccggtattgc ggtacccttg tacgcctgtt ttatactccc ttcccgtaac
1561 ttagacgcac aaaaccaagt tcaatagaag ggggtacaaa ccagtaccac cacgaacaag
1621 cacttctgtt tccccggtga tgtcgtatag actgcttgcg tggttgaaag cgacggatcc
1681 gttatccgct tatgtacttc gagaagccca gtaccacctc ggaatcttcg atgcgttgcg
1741 ctcagcactc aaccccagag tgtagcttag gctgatgagt ctggacatcc ctcaccggtg
1801 acggtggtcc aggctgcgtt ggcggcctac ctatggctaa cgccatggga cgctagttgt
1861 gaacaaggtg tgaagagcct attgagctac ataagaatcc tccggcccct gaatgcggct
1921 aatcccaacc tcggagcagg tggtcacaaa ccagtgattg gcctgtcgta acgcgcaagt
1981 ccgtggcgga accgactact ttgggtgtcc gtgtttcctt ttattttatt gtggctgctt
2041 atggtgacaa tcacagattg ttatcataaa gcgaattgga tactagtcca gtgtggtgga
2101 aatggtcagc aaaggagagg agctcttcac aggcgtggtc cccattctgg ttgagctcga
2161 cggggacgtc aatgggcaca agttctcagt gtcaggagaa ggcgaggggg atgccaccta
2221 tggcaaactt acactcaagt ttatctgcac tacaggtaag ctgccagtgc cttggccaac
2281 cctggtctgc actttgtcat ggggtgtgca atgttttgca cggtatcccg atcatatgaa
2341 acaacacgat ttctttaagt ctgccatgcc agagggctat gtgcaagaaa gaaccatctt
2401 tttcaaggac gatggcaact acaaaactag ggccgaggtg aagttcgaag gagacacatt
2461 ggtgaacagg atcgaactca aagggattga cttcaaagag gatggaaaca ttcttggaca
2521 caaactcgag tataactatt tttctgacaa cgtgtacatt actgctgaca aacaaaagaa
2581 tgggatcaag gccaatttta aaatccgcca caacattgaa gatggaggtg ttcagcttgc
2641 cgatcattac cagcaaaaca cacccatcgg tgacggtcct gtgcttttgc ctgacaacca
2701 ctatctttca acccaatcta agttgtccaa agatcctaat gagaaaagag atcatatggt
2761 cttgctcgag ttcgtgaccg cagcaggtat caccctgggc atggacgagc tctacaaggg
2821 gcatggcacg ggctcgggca ccgcctcgtc gatggaacag agaagagtga ccgacttttt
2881 cgccaggaga agacctggcc caccccggat cgcccctcca aaactggcct gcagaacccc
2941 ctctcctgcc agacctgccc tgagagcccc tgcctccgcc acctccggca gcagaaagag
3001 agccagacct cctgccgccc ctggcagaga tcaggccagg ccccctgccc gcgcggccgc
3061 gcggctgtcc gtggatgagg tgtccagccc tagcaccccc gaggcccccg atatccctgc
3121 ctgtcccagc cctggccaga aaatcaagaa gtaaacagtg gcggtgaaga ggcactttta
3181 agcggccgcg cccctctccc tccccccccc ctaacgttac tggccgaagc cgcttggaat
3241 aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg
3301 tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc
3361 tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt
3421 cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg
3481 acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac
3541 cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg
3601 tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg
3661 ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc
3721 cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg ataatatggc cacaaccccg
3781 ctcgagatga ccgagaactc cacctccgcc ccggcggcga agcccaaacg ggccaaggct
3841 tccaagaagt ccacggacca ccccaagtat tcagacatga tcgtggctgc tatccaggca
3901 gagaagaacc gtgccggctc ctcgcgccag tccatccaaa agtatatcaa gagccactac
3961 aaggtgggtg agaacgccga ctcccagatc aagttgtcca tcaagcgcct agtgaccacc
4021 ggtgttctca agcaaaccaa aggggtgggc gcctcggggt ccttcaggct ggccaagggc
4081 gatgagccca aaaggtcggt ggctttcaag aagaccaaga aggaagtcaa gaaagtggcc
4141 actccaaaga aggcagccaa gcccaagaag gctgcctcca aagccccaag caagaaaccc
4201 aaagccaccc ctgtcaagaa ggccaagaag aagccggctg ccacgcccaa gaaagccaaa
4261 aagcccaagg ttgtcaaagt caaaccagtc aaggcctcca aacccaagaa ggccaaaacc
4321 gtgaagccca aagccaagtc gagtgccaag agggggagca agaagaagtc cggactcaga
4381 tctggaggct ccggaggcat ggtctcaaag ggggaagaat tgtttaccgg tgtcgtgccc
4441 atcctcgttg agcttgatgg tgatgttaat ggtcacaagt tttccgtgtc aggggagggc
4501 gaaggggacg ccacatacgg gaaactgacc cttaagttta tctgtacaac cggaaaactg
4561 cccgtgccat ggccaacact tgtcacaaca ctttcctggg gacatcagtg ttttgccagg
4621 taccccgatc atatgaagca gcacgacttt ttcaagtccg ccatgcctga gggttatgtg
4681 caggagcgca caatcttttt taaggatgat ggtaactaca aaactcgggc agaggttaag
4741 ttcgagggtg acaccttggt caataggatt gagcttaagg gaatcgattt caaggaagac
4801 ggcaacattc tcgggcataa gctggagtac aactacttta gtgataacgt ttatatcacc
4861 gcagataagc agaaaaatgg catcaaagct aactttaaga ttagacataa cattgaggac
4921 ggcggggtgc agctggcaga tcattatcaa caaaacacac caatcggtga cggcccagtg
4981 ttgctcccag ataaccatta cttgtcaaca caatcaaaac tctcaaaaga cccaaatgag
5041 aaacgcgatc acatggttct tctggagttt gttaccgcag ccggaattac tctcggtatg
5101 gacgagctgt ataagtaaga attctgcaga tatccagcac agtggactag tagaccacct
5161 cccctgcgag ctaagctgga cagccaatga cgggtaagag agtgacattt ttcactaacc
5221 taagacagga gggccgtcag agctactgcc taatccaaag acgggtaaaa gtgataaaaa
5281 tgtatcactc caacctaaga caggcgcagc ttccgaggga tttgagatcc agacatgata
5341 agatacattg atgagtttgg acaaaccaaa actagaatgc agtgaaaaaa atgccttatt
5401 tgtgaaattt gtgatgctat tgccttattt gtaaccatta taagctgcaa taaacaagtt
5461 tgatatctat aacaagaaaa tatatatata ataagttatc acgtaagtag aacatgaaat
5521 aacaatataa ttatcgtatg agttaaatct taaaagtcac gtaaaagata atcatgcgtc
5581 attttgactc acgcggtcgt tatagttcaa aatcagtgac acttaccgca ttgacaagca
5641 cgcctcacgg gagctccaag cggcgactga gatgtcctaa atgcacagcg acggattcgc
5701 gctatttaga aagagagagc aatatttcaa gaatgcatgc gtcaatttta cgcagactat
5761 ctttctaggg ttaagaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
5821 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
5881 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc
5941 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact
6001 ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc
6061 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
6121 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga
6181 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
6241 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
6301 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
6361 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
6421 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
6481 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
6541 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
6601 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
6661 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
6721 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
6781 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
6841 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
6901 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
6961 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
7021 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
7081 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
7141 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
7201 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
7261 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
7321 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
7381 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
7441 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
7501 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
7561 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
7621 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
7681 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
7741 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
7801 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
7861 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
7921 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
7981 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
8041 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
8101 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
8161 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
8221 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
8281 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
8341 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat
8401 gattacgcca aggtcgactt aaccctagaa agataatcat attgtgacgt acgttaaaga
8461 taatcatgcg taaaattgac gcatgtgttt tatcggtctg tatatcgagg tttatttatt
8521 aatttgaata gatattaagt tttattatat ttacacttac atactaataa taaattcaac
8581 aaacaattta tttatgttta tttatttatt aaaaaaaaac aaaaactcaa aatttcttct
8641 ataaagtaac aaaactttta gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct
8701 attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt
8761 cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc
8821 tacaaatgtg gtatggctga ttatgatcct ctggagattt agccctccca cacataacca
8881 gagggcagca attcacgaat cccaactgcc gtcggctgtc catcactgtc cttcactatg
8941 gctttgatcc caggatgcag atcgagaagc acctgtcggc accgtccgca ggggctcaag
9001 atgcccctgt tctcatttcc gatcgcgacg atacaagtca ggttgccagc tgccgcagca
9061 gcagcagtgc ccagcaccac gagttctgca caaggtcccc cagtaaaatg atatacattg
9121 acaccagtga agatgcggcc gtcgctagag agagctgcgc tggcgacgct gtagtcttca
9181 gagatgggga tgctgttgat tgtagccgtt gctctttcaa tgagggtgga ttcttcttga
9241 gacaaaggct tggccatggg gccggggttc tcctccacgt cgccggcctg cttcagcagg
9301 ctgaagttgg tggcgccgct gcccccgggg agcatgtcaa ggtcaaaatc gtcaagagcg
9361 tcagcaggca gcatatcaag gtcaaagtcg tcaagggcat cggctgggag catgtctaag
9421 tcaaaatcgt caagggcgtc ggtcggcccg ccgctttcgc actttagctg tttctccagg
9481 ccacatatga ttagttccag gccgaaaagg aaggcaggtt cggctccctg ccggtcgaac
9541 agctcaattg cttgtttcag aagtgggggc atagaatcgg tggtaggtgt ctctctttcc
9601 tcttttgcta cttgatgctc ctgttcctcc aatacgcagc ccagtgtaaa gtggcccacg
9661 gcggacagag cgtacagtgc gttctccagg gagaagcctt gctgacacag gaacgcgagc
9721 tgattttcca gggtttcgta ctgtttctct gttgggcggg tgccgagatg cactttagcc
9781 ccgtcgcgat gtgagaggag agcacagcgg tatgacttgg cgttgttccg cagaaagtct
9841 tgccatgact cgccttccag ggggcaggag tgggtatgat gcctgtccag catctcgatt
9901 ggcagggcat cgagcagggc ccgcttgttc ttcacgtgcc agtacagggt aggctgctca
9961 actcccagct tttgagcgag tttccttgtc gtcaggcctt cgataccgac tccattgagt
10021 aattccagag cagagtttat gactttgctc ttgtccagtc tagacatctt atcgtcatcg
10081 tctttgtaat ccatggtggc ggatcccgcg tcacgacacc tgtgttctgg cggcaaaccc
10141 gttgcgaaaa agaacgttca cggcgactac tgcacttata tacggttctc ccccaccctc
10201 gggaaaaagg cggagccagt acacgacatc actttcccag tttaccccgc gccaccttct
10261 ctaggcaccg gttcaattgc cgacccctcc ccccaacttc tcggggactg tgggcgatgt
10321 gcgctctgcc cactgacggg caccggagcc actcgagtgg aatt
//
Xlone-mT2(T203G)-Gem1(1-110)-IRES-Cdt1(1-100)(Cy-)-mT2(T62C)载体图谱质粒图谱pdf版和Xlone-mT2(T203G)-Gem1(1-110)-IRES-Cdt1(1-100)(Cy-)-mT2(T62C)载体序列质粒序列等相关资料下载
Xlone-mT2(T203G)-Gem1(1-110)-IRES-Cdt1(1-100)(Cy-)-mT2(T62C)质粒载体应用举例
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