| 质粒名称: | LEGO-AP1x3-GM55 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 217414 |
| 存储实验室: | Peter Cockerill |
|
载体骨架基本信息 |
|
| 载体名称: | LeGO-iG |
| 载体出品公司: | Boris Fehse (Addgene #27358) |
| 完整质粒大小: | 9767bp |
| 载体类型: | 哺乳细胞表达, Lentiviral, Luciferase |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | NEB Stable |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | Luciferase, GFP、SV40 PolyA sites, 3x AP-1 sites, Human -55 to +28 minimal CSF2 promoter, Promega pGL3 LUC+, IRES-EGFP |
| 物种来源: |
H. sapiens (human); Firefly
Promoter3 AP-1 sites, Human -55 to +28 minimal CSF2 promoter
|
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | unknown (unknown if destroyed) |
| 3’克隆位点: | unknown (unknown if destroyed) |
| 5’测序引物 | n/a |
|
文献引用方法 |
|
| 材料和方法部分: | LEGO-AP1x3-GM55 was a gift from Peter Cockerill (Addgene plasmid # 217414 ; http://n2t.net/addgene:217414 ; RRID:Addgene_217414) |
买家导航
LEGO-AP1x3-GM55载体质粒基本信息
LEGO-AP1x3-GM55质粒图谱载体图谱和LEGO-AP1x3-GM55载体序列质粒序列多克隆位点信息
LEGO-AP1x3-GM55质粒载体简介
LEGO-AP1x3-GM55质粒序列载体序列
LOCUS LEGO-AP1x3-GM55 9767 bp DNA circular SYN 27-AUG-2024
DEFINITION Luciferase expression vector with 3 AP-1 sites and the minimal
GM-CSF promoter. Alias: LEGO-GM55-AP-1x3.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9767)
TITLE Cockerill Lab Luciferase Plasmids 2024
REFERENCE 2 (bases 1 to 9767)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 27, 2024 from SnapGene Server 7.0.3
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..9767
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind complement(47..66)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 238..617
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 619..817
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 772..792
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
LTR 835..1015
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 1062..1187
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1684..1917
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 2102..2146
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 2444..2561
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
primer_bind 2721..2743
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
polyA_signal 2874..3008
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
polyA_signal 3102..3236
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
regulatory 3518..3527
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 3524..5176
/codon_start=1
/gene="luc+"
/product="firefly luciferase"
/label=luciferase
/note="enhanced luc+ version of the luciferase gene"
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
primer_bind complement(3590..3608)
/label=LucNrev
/note="Firefly luciferase, reverse primer"
misc_feature 5214..5800
/label=IRES2
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
primer_bind complement(5381..5398)
/label=IRES reverse
/note="IRES internal ribosome entry site, reverse primer.
Also called pCDH-rev"
primer_bind 5608..5627
/label=IRES-F
/note="IRES internal ribosome entry site, forward primer"
CDS 5801..6520
/codon_start=1
/product="the original enhanced GFP (Yang et al., 1996)"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
CDS 5801..6517
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(5846..5867)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(6107..6126)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 6454..6475
/label=EGFP-C
/note="EGFP, forward primer"
protein_bind complement(6567..6600)
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (ATGTATGC) (Shaw et al., 2021)."
misc_feature 6656..7244
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(6709..6729)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(7127..7138)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
primer_bind complement(7247..7263)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(7248..7264)
/label=pBluescriptKS
/note="For pBluescript vector"
LTR 7773..7953
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
rep_origin complement(8015..8603)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(8095..8114)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(8774..9634)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 9397..9416
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(9635..9739)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
1501 aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg gaggaggaga
1561 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
1621 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
1681 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
1741 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
1801 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
1861 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
1921 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
1981 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
2041 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
2101 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
2161 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
2221 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
2281 attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga
2341 agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg gatcggcact
2401 gcgtgcgcca attctgcaga caaatggcag tattcatcca caattttaaa agaaaagggg
2461 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
2521 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
2581 gcagagatcc agtttggtta gtaccgggcc cgctctagac cgggtcatgg ctgcgccccg
2641 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
2701 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
2761 gaaacgcgcg aggcaggatc agccatatca catttgtaga ggttttactt gctttaaaaa
2821 acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttaacttgt
2881 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
2941 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
3001 tctggatcag ccatatcaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc
3061 tccccctgaa cctgaaacat aaaatgaatg caattgttgt taacttgttt attgcagctt
3121 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
3181 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatccaag
3241 ctcagatctc gagatctgca aggatgagtc aagctgcggg tgatccagat ctgcaaggat
3301 gagtcaagct gcgggtgatc cagatctgca aggatgagtc aagctgcggg tgatccagat
3361 ctgggcccgg atcctgcagg gggtcgacaa gcttcgcgag gtcaccatta atcatttcct
3421 ctgtgtattt aagagctctt ttgccagtga gcccagtaca cagagagaaa ggctaaagtt
3481 ctgtcgacaa gcttggcatt ccggtactgt tggtaaagcc accatggaag acgccaaaaa
3541 cataaagaaa ggcccggcgc cattctatcc gctggaagat ggaaccgctg gagagcaact
3601 gcataaggct atgaagagat acgccctggt tcctggaaca attgctttta cagatgcaca
3661 tatcgaggtg gacatcactt acgctgagta cttcgaaatg tccgttcggt tggcagaagc
3721 tatgaaacga tatgggctga atacaaatca cagaatcgtc gtatgcagtg aaaactctct
3781 tcaattcttt atgccggtgt tgggcgcgtt atttatcgga gttgcagttg cgcccgcgaa
3841 cgacatttat aatgaacgtg aattgctcaa cagtatgggc atttcgcagc ctaccgtggt
3901 gttcgtttcc aaaaaggggt tgcaaaaaat tttgaacgtg caaaaaaagc tcccaatcat
3961 ccaaaaaatt attatcatgg attctaaaac ggattaccag ggatttcagt cgatgtacac
4021 gttcgtcaca tctcatctac ctcccggttt taatgaatac gattttgtgc cagagtcctt
4081 cgatagggac aagacaattg cactgatcat gaactcctct ggatctactg gtctgcctaa
4141 aggtgtcgct ctgcctcata gaactgcctg cgtgagattc tcgcatgcca gagatcctat
4201 ttttggcaat caaatcattc cggatactgc gattttaagt gttgttccat tccatcacgg
4261 ttttggaatg tttactacac tcggatattt gatatgtgga tttcgagtcg tcttaatgta
4321 tagatttgaa gaagagctgt ttctgaggag ccttcaggat tacaagattc aaagtgcgct
4381 gctggtgcca accctattct ccttcttcgc caaaagcact ctgattgaca aatacgattt
4441 atctaattta cacgaaattg cttctggtgg cgctcccctc tctaaggaag tcggggaagc
4501 ggttgccaag aggttccatc tgccaggtat caggcaagga tatgggctca ctgagactac
4561 atcagctatt ctgattacac ccgaggggga tgataaaccg ggcgcggtcg gtaaagttgt
4621 tccatttttt gaagcgaagg ttgtggatct ggataccggg aaaacgctgg gcgttaatca
4681 aagaggcgaa ctgtgtgtga gaggtcctat gattatgtcc ggttatgtaa acaatccgga
4741 agcgaccaac gccttgattg acaaggatgg atggctacat tctggagaca tagcttactg
4801 ggacgaagac gaacacttct tcatcgttga ccgcctgaag tctctgatta agtacaaagg
4861 ctatcaggtg gctcccgctg aattggaatc catcttgctc caacacccca acatcttcga
4921 cgcaggtgtc gcaggtcttc ccgacgatga cgccggtgaa cttcccgccg ccgttgttgt
4981 tttggagcac ggaaagacga tgacggaaaa agagatcgtg gattacgtcg ccagtcaagt
5041 aacaaccgcg aaaaagttgc gcggaggagt tgtgtttgtg gacgaagtac cgaaaggtct
5101 taccggaaaa ctcgacgcaa gaaaaatcag agagatcctc ataaaggcca agaagggcgg
5161 aaagatcgcc gtgtaattct agggcggccg ctacgtaaat tccgcccccc ccccccctct
5221 ccctcccccc cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt
5281 gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct
5341 ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa
5401 ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg
5461 tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc
5521 caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg
5581 agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg
5641 aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtgcacatgc
5701 tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg
5761 gttttccttt gaaaaacacg atgataatat ggccacaacc atggtgagca agggcgagga
5821 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
5881 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
5941 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta
6001 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc
6061 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
6121 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
6181 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
6241 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa
6301 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
6361 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc
6421 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
6481 cgccgggatc actctcggca tggacgagct gtacaagtaa agcggccggc cgccagcaca
6541 gtggtcgaaa ttcgtcgagg gacctaataa cttcgtatag catacattat acgaagttat
6601 acatgtttaa gggttccggt tccactaggt acaattcgat atcaagctta tcgataatca
6661 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
6721 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
6781 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
6841 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
6901 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
6961 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
7021 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg
7081 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
7141 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
7201 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgat accgtcgacc
7261 tcgatcgaga cctagaaaaa catggagcaa tcacaagtag caatacagca gctaccaatg
7321 ctgattgtgc ctggctagaa gcacaagagg aggaggaggt gggttttcca gtcacacctc
7381 aggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
7441 aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatatc cttgatctgt
7501 ggatctacca cacacaaggc tacttccctg attggcagaa ctacacacca gggccaggga
7561 tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag caagagaagg
7621 tagaagaagc caatgaagga gagaacaccc gcttgttaca ccctgtgagc ctgcatggga
7681 tggatgaccc ggagagagaa gtattagagt ggaggtttga cagccgccta gcatttcatc
7741 acatggcccg agagctgcat ccggactgta ctgggtctct ctggttagac cagatctgag
7801 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
7861 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
7921 gaccctttta gtcagtgtgg aaaatctcta gcagcatgtg agcaaaaggc cagcaaaagg
7981 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
8041 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
8101 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
8161 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
8221 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
8281 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
8341 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
8401 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
8461 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
8521 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
8581 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
8641 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
8701 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
8761 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
8821 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
8881 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
8941 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
9001 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
9061 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
9121 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
9181 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
9241 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
9301 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
9361 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
9421 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
9481 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
9541 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
9601 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
9661 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
9721 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgac
//
