| 质粒名称: | pAAV-CAG-eRFP2-P2A-EGFP |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000711 |
| 产品用途: | AAV, experess eRFP2 and EGFP in cytoplasm of mammalian cells |
| 存储实验室: | KIRYL PIATKEVICH Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV |
| 表达系统: | 腺病毒，哺乳动物表达 |
| 启动子: | CAG |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | TOP10 |
|
插入基因信息 |
|
| 插入基因名称: | eRFP2, EGFP |
| 物种来源: | Synthetic |
| 密码子优化: | 未优化 |
买家导航
pAAV-CAG-eRFP2-P2A-EGFP载体质粒基本信息
pAAV-CAG-eRFP2-P2A-EGFP质粒图谱载体图谱和pAAV-CAG-eRFP2-P2A-EGFP载体序列质粒序列多克隆位点信息
pAAV-CAG-eRFP2-P2A-EGFP质粒载体简介
pAAV-CAG-eRFP2-P2A-EGFP质粒序列载体序列
LOCUS pAAV-CAG-eRFP2-P2A-EGFP 6244 bp DNA circular SYN 22-MAR-2023
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6244)
AUTHORS Triple Threat
TITLE Direct Submission
JOURNAL Exported Apr 30, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6244
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 279..556
/label=chicken beta-actin promoter
primer_bind 919..941
/label=Seq-F
primer_bind 955..992
/label=eRFP2-F2
primer_bind 966..1002
/label=eRFP2-F
regulatory 977..982
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 983..1693
/codon_start=1
/label=eRFP2
/translation="MVSKGEELLKENMRMKVDMEGSINGHQFKCTGEGEGRPYEGVQTM
RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPADIPDFFKQSFPEGFTWERVTRYEDGG
VVTVTQDTSLEDGELVYNVKVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTD
IALKVDDGGHLHCNFVTTYRSKKTVGNIKMPGVHAVDHRLERIEESDNETYVVQREVAV
AKYSNLGGGMDELYK"
primer_bind complement(1674..1710)
/label=eRFP2-R
primer_bind complement(1676..1716)
/label=eRFP2-R2
CDS 1718..1774
/codon_start=1
/product="2A peptide from porcine teschovirus-1
polyprotein"
/label=P2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="ATNFSLLKQAGDVEENPGP"
CDS 1787..2503
/codon_start=1
/product="the original enhanced GFP (Yang et al., 1996)"
/label=EGFP
/note="mammalian codon-optimized"
/translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
FVTAAGITLGMDELYK"
primer_bind complement(2481..2500)
/label=eRFP2-R
primer_bind complement(2483..2500)
/label=eRFP2-R2
misc_feature 2528..3116
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
CDS complement(2999..3010)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
polyA_signal 3159..3280
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(3808..4396)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(4567..5427)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(5428..5532)
/gene="bla"
/label=AmpR promoter
rep_origin complement(5558..6013)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
ORIGIN
1 tctacgtagc catgctctag gaagagtacc attgacgtca ataatgacgt atgttcccat
61 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
121 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
181 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
241 gcagtacatc tacgtattag tcatcgctat taccatggtc gaggtgagcc ccacgttctg
301 cttcactctc cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta
361 attattttgt gcagcgatgg gggcgggggg gggggggggg cgcgcgccag gcggggcggg
421 gcggggcgag gggcggggcg gggcgaggcg gagaggtgcg gcggcagcca atcagagcgg
481 cgcgctccga aagtttcctt ttatggcgag gcggcggcgg cggcggccct ataaaaagcg
541 aagcgcgcgg cgggcgggag tcgctgcgcg ctgccttcgc cccgtgcccc gctccgccgc
601 cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta ctcccacagg tgagcgggcg
661 ggacggccct tctcctccgg gctgtaatta gcgcttggtt taatgacggc ttgtttcttt
721 tctgtggctg cgtgaaagcc ttgaggggct ccgggagggc cctttgtgcg gggggagcgg
781 ctcggggctg tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg
841 gcttctggcg tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct
901 ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat tttggcaaag
961 aattggatcc ggtaccgcca ccatggtgtc taagggcgaa gagctgctca aggaaaatat
1021 gcgtatgaag gtggacatgg aaggttcgat caacggccac caatttaaat gcacaggtga
1081 aggagaaggc agaccgtacg agggagtgca aaccatgagg atcaaagtca tcgagggagg
1141 acccctgcca tttgcctttg acattcttgc cacgtcgttc atgtacggca gcaggacctt
1201 tatcaagtac ccggccgaca tccctgattt ctttaaacag tcctttcctg agggttttac
1261 ttgggaaaga gttacgagat acgaagatgg tggagtcgtc accgtcacgc aggacaccag
1321 ccttgaggat ggcgagctcg tctacaacgt caaggtcaga ggggtaaact ttccctccaa
1381 tggtcccgtg atgcagaaga agaccaaggg ttgggagcct aacacagaga tgatgtatcc
1441 agcagatggt ggtctgagag gatacaccga catcgcactg aaagttgatg atggtggcca
1501 tctgcactgc aacttcgtga caacttacag gtcaaaaaag accgtcggga acatcaagat
1561 gcccggtgtc catgccgttg atcaccgcct ggaaaggatc gaggagagtg acaatgaaac
1621 ctacgtagtg caaagagaag tggcagttgc caaatacagc aacctcggtg gtggcatgga
1681 cgagctgtac aagcgaccgg ttcacgtggg aagtggggct acaaacttct cactgctgaa
1741 acaggccggg gatgtggagg aaaacccagg accacacgtg actagtgtga gcaagggcga
1801 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
1861 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
1921 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
1981 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
2041 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
2101 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
2161 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
2221 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
2281 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
2341 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
2401 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
2461 cgccgccggg atcactctcg gcatggacga gctgtacaag taagaattcg atatcaagct
2521 tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
2581 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
2641 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
2701 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
2761 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
2821 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
2881 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg
2941 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
3001 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
3061 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg
3121 ataccgtcga cccgggcggc cgcttcgagc agacatgata agatacattg atgagtttgg
3181 acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat
3241 tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca
3301 ttttatgttt caggttcagg gggagatgtg ggaggttttt taaagcaagt aaaacctcta
3361 caaatgtggt aaaatcgata aggatcttcc tagagcatgg ctacgtagat aagtagcatg
3421 gcgggttaat cattaactac aaggaacccc tagtgatgga gttggccact ccctctctgc
3481 gcgctcgctc gctcactgag gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc
3541 gggcggcctc agtgagcgag cgagcgcgca gctgcattaa tgaatcggcc aacgcgcggg
3601 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
3661 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
3721 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
3781 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
3841 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
3901 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
3961 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
4021 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
4081 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
4141 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
4201 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
4261 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
4321 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
4381 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
4441 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
4501 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
4561 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
4621 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
4681 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
4741 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
4801 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
4861 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
4921 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
4981 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
5041 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
5101 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
5161 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
5221 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
5281 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
5341 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
5401 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
5461 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
5521 aggggttccg cgcacatttc cccgaaaagt gccacctaaa ttgtaagcgt taatattttg
5581 ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc
5641 ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt
5701 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc
5761 tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg
5821 tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga
5881 aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg
5941 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg
6001 ctacagggcg cgtcccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
6061 cgggcctctt cgctattacg ccagctgcgc gctcgctcgc tcactgaggc cgcccgggca
6121 aagcccgggc gtcgggcgac ctttggtcgc ccggcctcag tgagcgagcg agcgcgcaga
6181 gagggagtgg ccaactccat cactaggggt tccttgtagt taatgattaa cccgccatgc
6241 tact
//
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