pAR18_StrepI_mFASm_H8_pET22b

LOCUS        pAR18_StrepI_mFASm_H8_pET22b       12983 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Expresses murine type I fatty acid synthase (FASN) in Escherichia 
            coli. N-terminal Twin-Strep tag; C-terminal H8-tag; in pET22b 
            vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12983)
  AUTHORS   Rittner A, Paithankar KS, Drexler DJ, Himmler A, Grininger M
  TITLE     Probing the modularity of megasynthases by rational engineering of a
            fatty acid synthase Type I.
  JOURNAL   Protein Sci. 2019 Feb;28(2):414-428. doi: 10.1002/pro.3550. Epub 
            2018 Dec 20.
  PUBMED    30394635
REFERENCE   2  (bases 1 to 12983)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.biofeng.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/pro.3550"; 
            journalName: "Protein Sci"; date: "2019-02"; volume: "28"; issue: 
            "2"; pages: "414-428"
COMMENT     Sequence Label: pAR18_StrepI_mFASm_H8_pET22b
FEATURES             Location/Qualifiers
     source          1..12983
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     154..173
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     257..276
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        257..275
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    276..300
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             315..337
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             join(348..353,354..377,378..413,414..437)
                     /codon_start=1
                     /product="two Strep-Tag(R)II moieties connected by a linker
                     for enhanced binding to Strep-Tactin(R), an engineered form
                     of streptavidin (Schmidt et al., 2013)"
                     /label=Twin-Strep-tag
                     /translation="SAWSHPQFEKGGGSGGGSGGSAWSHPQFEK"
     CDS             7965..7988
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     primer_bind     complement(8041..8059)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      8055..8102
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      8139..8594
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8226..8245)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8436..8457
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8620..8724
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8725..8793,8794..9585)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8943..8962)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9756..10344
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10245..10264
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10498..10515
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    10530..10669
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(10595..10614)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10755..10777
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(10771..10962)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(11771..12853)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     12815..12834
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(12854..12931)
                     /gene="lacI"
                     /label=lacI promoter
ORIGIN
        1 tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc
       61 gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat
      121 gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc
      181 aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat
      241 ctcgatcccg cgaaattaat acgactcact ataggggaat tgtgagcgga taacaattcc
      301 cctctagaaa taattttgtt taactttaag aaggagatat acatatgagc gcttggagcc
      361 atccacaatt tgagaagggt ggaggttctg gcggtggatc gggaggttca gcgtggagcc
      421 acccgcagtt cgaaaaaggc gccggatccg aggaggtggt gatagccggt atgtcgggga
      481 agttgcccga gtcagagaac ctacaggagt tctgggccaa cctcattggt ggtgtggaca
      541 tggtcacaga tgatgacagg agatggaagg ctgggctcta tggattaccc aagcggtctg
      601 gaaagctgaa ggatctctcc aagttcgacg cctccttttt tggggtccac cccaagcagg
      661 cacacacaat ggacccccag cttcggctgc tgttggaagt cagctatgaa gcaattgtgg
      721 atggaggtat caacccagcc tcactccgag gaacgaacac tggcgtctgg gtgggtgtga
      781 gtggttcaga ggcatccgag gcccttagca gagatcccga gacgcttctg ggctacagca
      841 tggtgggctg ccagcgtgca atgatggcca accggctctc tttcttcttc gacttcaaag
      901 gaccaagcat tgccctggac acagcctgct cctccagctt gctggcacta cagaatgcct
      961 accaggccat ccgtagtggg gaatgccccg cggcccttgt gggtgggatc aacctgctcc
     1021 tgaagccgaa cacctctgtg cagttcatga agctgggcat gctcagcccg gacggcacct
     1081 gcagatcctt tgatgattca gggagtggat attgtcgctc tgaggctgtt gtagcagttc
     1141 tgctgactaa gaagtccctg gctcggcggg tctatgccac gattctgaat gccggcacca
     1201 atacagatgg cagcaaggag caaggtgtaa cattcccctc tggagaagtc caagaacaac
     1261 tcatctgctc tctgtatcag ccagctggtc tggccccgga gtcgcttgag tatattgaag
     1321 cccatggcac gggcaccaag gtgggtgacc cccaggaact gaatggcatt actcggtccc
     1381 tgtgcgcctt ccgccaggcc cctctgttaa ttggctccac caaatccaac atgggacacc
     1441 ctgagcctgc ctctgggctt gcagccctga ccaaggtgct gttatccctg gagcatgggg
     1501 tctgggcccc taacctgcac ttccacaacc ccaaccctga gatcccagca cttcttgatg
     1561 ggcggctgca ggtggtcgat aggcccctgc ctgttcgtgg tggcaacgtg ggcatcaact
     1621 catttggctt cggaggctcc aatgttcatg tcatcctcca gcccaacaca cggcaggccc
     1681 ctgcgcccac tgcacacgct gcccttcccc atttgctgca cgccagtgga cgcaccttag
     1741 aggcagtgca ggacctgctg gaacagggcc gccagcacag ccaggacctg gcctttgtga
     1801 gcatgctcaa tgacattgcg gcaaccccta cagcagccat gcccttcagg ggttacactg
     1861 tgctaggtgt tgagggccgt gtccaagaag tgcagcaagt gtccaccaac aagcgcccac
     1921 tctggttcat ctgctcaggg atgggcacgc agtggcgcgg gatggggctg agcctcatgc
     1981 gcctggacag cttccgtgag tctatcctgc gctccgatga ggctgtgaag ccgttgggag
     2041 tgaaagtgtc agatctgctg ttgagcacag atgagcgcac ctttgatgac atcgtgcatg
     2101 cctttgtgag cctcactgcc atccagattg ccctcatcga cctactgact tctgtgggac
     2161 tgaaacctga cggcatcatt gggcactcct tgggagaggt tgcctgtggc tatgcagatg
     2221 gctgtctctc ccagagagag gctgtgcttg cagcttactg gcgaggccag tgcatcaaag
     2281 atgcccacct cccgcctgga tccatggcag ctgttggttt gtcctgggag gaatgtaaac
     2341 agcgctgccc cgctggcgtg gtgcctgcct gccacaactc tgaggacacc gtgaccatct
     2401 ctggacctca ggctgcagtg aatgaatttg tggagcagct aaagcaagaa ggtgtgtttg
     2461 ccaaggaggt acgaacagga ggcctggctt tccactccta cttcatggaa ggaattgccc
     2521 ccacattgct gcaggctctc aagaaggtga tccgggaacc acggccgcgc tcggctcgat
     2581 ggctcagcac ctctatccct gaggcccagt ggcagagcag cctggcccgc acatcttctg
     2641 ccgagtacaa tgtcaacaac ctggtgagcc ctgtgctctt ccaggaagca ctgtggcaca
     2701 tccctgagca tgccgtggtg ctggagattg cgccccacgc actgttgcag gctgtcctga
     2761 agcgaggcgt gaagtccagc tgcaccatca ttcccttgat gaagagggat cataaagata
     2821 acttggagtt ctttctcacc aaccttggca aggtgcacct cacaggcatc aatgtcaacc
     2881 ctaacgcctt gttcccacct gtggagttcc cggctccccg agggactcct ctcatctccc
     2941 ctcacatcaa gtgggaccac agtcagactt gggatgtccc ggttgctgag gacttcccaa
     3001 acggctccag ctcctcctct gctacagtct acagcatcga cgccagtcct gagtcgcccg
     3061 accactacct ggtagaccac tgcattgacg gccgggtcat cttccctggc actggctacc
     3121 tgtgcctggt gtggaagaca ctggctcgca gcctgggctt gtccctagaa gagacccctg
     3181 tggtatttga gaatgtgtcg tttcatcagg ccactatact acccaagaca ggaaccgtgg
     3241 cgctggaggt gaggctgcta gaggcctccc atgcctttga ggtgtctgac actggcaatc
     3301 tgattgtgag cggaaaagtg tacctgtggg aagacccgaa ctccaagtta ttcgaccacc
     3361 cagaagtccc aacaccccct gagtctgcat cggtctcccg cctgacccag ggagaagtat
     3421 acaaggagct gcggctgcgt ggctatgatt atggccctca gttccagggc atctgtgagg
     3481 ccacccttga aggtgaacaa ggcaagctgc tctggaaaga taactgggtg accttcatgg
     3541 acacaatgct gcaggtatcc attctgggtt ctagccagca gagtctacag ctacctaccc
     3601 gtgtgaccgc catctatatc gaccctgcca cccaccgtca gaaggtgtac aggctgaagg
     3661 aggacactca agtggctgat gtgacaacga gccgctgtct gggcataacg gtctctggtg
     3721 gtatccacat ctcaagacta cagacgacag caacctcacg gcggcagcaa gaacagctgg
     3781 tccccacctt ggaaaagttc gttttcacac cgcacatgga ggctgagtgc ctgtctgaga
     3841 gcactgccct gcagaaggag ctgcaactgt gcaagggtct ggcacgggct ctgcagacca
     3901 aggccaccca gcaagggctg aaggcggcaa tgcttgggca agaggaccct ccacagcacg
     3961 ggctgcctcg actcctggca gctgcttgcc agttgcagct caacgggaac ctgcagctgg
     4021 agctgggaga agcgctggct caagagaggc tcctgctgcc agaagaccct ctgatcagtg
     4081 gcctcctcaa ctcccaggcc ctcaaggcct gcgtagacac agccctggag aacttgtcta
     4141 ctctcaagat gaaggtggca gaggtgctgg ctggagaagg ccacttgtat tcccgaatcc
     4201 cggcactgct caacacccag cccatgctac aactggaata cacagccacc gaccggcacc
     4261 cccaggccct gaaggatgtt cagaccaaac tgcagcagca tgatgtggcg cagggccagt
     4321 ggaacccttc cgaccctgcg cccagcagcc tgggtgccct tgaccttctg gtgtgcaact
     4381 gtgcattagc caccctgggg gatccagcct tggccctgga caacatggta gctgccctca
     4441 aggaaggtgg tttcctgcta gtgcacacag tgctcaaagg acatgccctt ggggagaccc
     4501 tggcctgcct accctctgag gtgcagcctg cgcccagcct cctaagccag gaggagtggg
     4561 agagcctgtt ctcgaggaag gcactacacc tggtgggcct taaaaggtcc ttctacggta
     4621 ctgcgctgtt cctgtgccgg cgagccatcc cacaggagaa acctatcttc ctgtctgtgg
     4681 aggataccag cttccagtgg gtggactctc tgaagagcac tctggccacg tcctcctccc
     4741 agcctgtgtg gctaacggcc atggactgcc ccacctcggg tgtggtgggt ttggtgaatt
     4801 gtctccgaaa agagccgggt ggacaccgga ttcggtgtat cctgctgtcc aacctcagca
     4861 acacatctca cgcccccaag ttggaccctg gctctccaga gctacagcag gtgctaaagc
     4921 atgacctcgt gatgaacgtg taccgggacg gggcctgggg tgccttccgt cacttccagt
     4981 tagagcagga caagcccaag gagcagacag cgcatgcctt tgtaaacgtc ctcacccgag
     5041 gggacctcgc ctccatccgc tgggtctcct cccccctgaa gcacacgcag ccctcgagct
     5101 caggagcaca gctctgcact gtctactacg cctcactgaa cttccgagac atcatgctgg
     5161 ccacgggcaa gctgtcccct gatgccattc caggtaaatg ggccagccga gactgcatgc
     5221 tcggcatgga gttctcaggc cgggataggt gtggccggcg tgtgatgggg ctggttcctg
     5281 cagaaggcct ggccacctca gtcctgctat catctgactt cctctgggat gtaccctcca
     5341 gctggaccct ggaggaggcg gcctctgtgc ccgtcgtcta taccactgct tactactcgt
     5401 tagtggttcg cgggcgcatc cagcgtgggg agaccgtgct catccactca ggttcaggtg
     5461 gtgtgggcca agcggccatt tccattgccc tcagtctggg ctgccgcgtc ttcaccactg
     5521 tgggctctgc agagaagcga gcatacctcc aggccaggtt ccctcagctt gatgacacca
     5581 gctttgccaa ctcgagggac acatcatttg agcagcacgt gttactgcac acaggtggca
     5641 aaggggtcga cctggtcctc aactcactgg cagaagagaa gctgcaggcc agtgtgcggt
     5701 gcttggctca gcatggtcgc ttcttagaga ttggcaaatt tgatctttct aacaaccacc
     5761 ctctgggcat ggctatcttc ttgaagaacg tcactttcca tgggatcctg ctggacgccc
     5821 tttttgagga ggccaatgac agctggcggg aggtggcggc actcctgaag gctggcattc
     5881 gtgatggagt cgtgaagccc ctcaagtgca cagtgtttcc caaggcccag gtggaagatg
     5941 ccttccgcta catggctcag gggaaacaca ttggcaaagt ccttgtccag gtacgggagg
     6001 aggagcctga ggctgtgctg ccaggggctc agcccaccct gatttctgcc atctccaaga
     6061 ccttctgccc agcccataag agttacatca tcactggtgg cctaggtggc tttggcctgg
     6121 agctggcccg gtggctcgtg cttcgcggag cccagaggct tgtgctgact tcccgatctg
     6181 gaatccgcac cggctaccaa gccaagcaca ttcgggagtg gagacgccag ggcatccaag
     6241 tgctcgtgtc aacaagcaac gtgagctcac tggagggggc ccgtgctctc atcgccgaag
     6301 ccacaaagct ggggcccgtt gggggtgtct tcaacctggc catggttttg agggatgcca
     6361 tgctggagaa ccagacccca gagctcttcc aggatgtcaa caagcccaaa tacaatggca
     6421 ccctgaacct tgacagggca acccgggaag cctgccctga gctggactac tttgtggcct
     6481 tctcctctgt aagctgcggg cgtggtaatg ctggccaaac taactacggc ttcgccaact
     6541 ctaccatgga gcgtatatgt gaacagcgca ggcacgatgg cctcccaggc cttgccgtgc
     6601 agtggggtgc cattggtgac gtgggcattg tcctggaagc gatgggcacc aatgacacag
     6661 tcatcggagg tacgctgcct cagcgcatct cctcctgcat ggaggtactg gacctcttcc
     6721 tgaatcagcc ccacgcagtc ctgagcagct ttgtgctggc agagaagaaa gctgtggccc
     6781 atggggacgg ggacacccag agggatctgg tgaaagctgt agcacacatc ctaggcatcc
     6841 gagacctcgc aggtattaac ctggacagca cgctggcaga cctcggcctg gactcgctca
     6901 tgggtgtgga agttcgtcag atcctggaac gagaacacga tctggtgctg cccatgcgtg
     6961 aggtgcggca gctcacgctg cggaaacttc aggaaatgtc ctccaagact gactcggcta
     7021 ctgacacgac agcccccaag tccaggagtg acacgtctct gaagcagaac caactgaacc
     7081 tgagcacact gctggtgaac cctgagggtc ctaccctaac ccagctcaac tcggtgcaga
     7141 gctctgagcg gcctctgttc cttgtgcacc ccattgaggg ttccaccacc gtgttccaca
     7201 gtctggctgc caagctcagt gtgcccacct acggcctgca gtgcacccaa gctgcccccc
     7261 tggatagcat tccgaacctg gctgcctact acatagattg catcaagcaa gtgcagcctg
     7321 agggacccta ccgcatagct gggtactcat ttggagcctg tgtagccttc gagatgtgct
     7381 cccagctgca ggcccagcag ggcccagccc cgacccacaa caacctcttc ctgtttgacg
     7441 gctcacacac ctacgtgttg gcctacaccc agagctaccg ggcaaagatg accccaggct
     7501 gtgaagccga ggccgaggct gaggccttat gcttcttcat aaagcagttt cttgatgtgg
     7561 aacacagcaa ggtgctggag gccctgctgc cactgaagag cctggaagat cgggtggctg
     7621 cctccgtgga ccttatcact aagagtcacc acagcctgga ccgccgagag ctgagctttg
     7681 ctgccgtgtc cttctaccac aagctccggg cagctgatca gtataagccc aaggccaagt
     7741 accatggcaa cgtgacactg ctgcgtgcca agacaggcgg cacctatggc gaggacttgg
     7801 gtgctgacta caacctctcc caggtgtgtg acgggaaggt gtctgtgcac atcattgagg
     7861 gtgaccaccg cacactgctg gagggcagtg gcctggaatc catcatcaac atcatccata
     7921 gctccctggc tgagccacga gtgagtgtac gggagggcct cgagcatcat caccaccacc
     7981 accaccactg agatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca
     8041 ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt
     8101 tgctgaaagg aggaactata tccggattgg cgaatgggac gcgccctgta gcggcgcatt
     8161 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     8221 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     8281 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     8341 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     8401 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     8461 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     8521 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
     8581 aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     8641 tttttctaaa tacattcaaa tatgtattcg ctcatgagac aataaccctg ataaatgctt
     8701 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     8761 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     8821 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     8881 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     8941 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     9001 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     9061 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     9121 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     9181 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     9241 aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta
     9301 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     9361 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     9421 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     9481 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     9541 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     9601 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     9661 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     9721 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     9781 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     9841 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     9901 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     9961 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
    10021 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
    10081 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
    10141 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
    10201 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
    10261 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
    10321 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
    10381 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
    10441 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
    10501 gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg
    10561 tgcggtattt cacaccgcaa tggtgcactc tcagtacaat ctgctctgat gccgcatagt
    10621 taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc
    10681 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
    10741 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
    10801 cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc
    10861 ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat
    10921 aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg
    10981 gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg
    11041 ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt
    11101 atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac
    11161 agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat
    11221 ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat
    11281 tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg
    11341 tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa
    11401 cgacaggagc acgatcatgc gcacccgtgg ggccgccatg ccggcgataa tggcctgctt
    11461 ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat
    11521 tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc
    11581 gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt
    11641 cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa
    11701 ggctctcaag ggcatcggtc gagatcccgg tgcctaatga gtgagctaac ttacattaat
    11761 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
    11821 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgccagggtg gtttttcttt
    11881 tcaccagtga gacgggcaac agctgattgc ccttcaccgc ctggccctga gagagttgca
    11941 gcaagcggtc cacgctggtt tgccccagca ggcgaaaatc ctgtttgatg gtggttaacg
    12001 gcgggatata acatgagctg tcttcggtat cgtcgtatcc cactaccgag atatccgcac
    12061 caacgcgcag cccggactcg gtaatggcgc gcattgcgcc cagcgccatc tgatcgttgg
    12121 caaccagcat cgcagtggga acgatgccct cattcagcat ttgcatggtt tgttgaaaac
    12181 cggacatggc actccagtcg ccttcccgtt ccgctatcgg ctgaatttga ttgcgagtga
    12241 gatatttatg ccagccagcc agacgcagac gcgccgagac agaacttaat gggcccgcta
    12301 acagcgcgat ttgctggtga cccaatgcga ccagatgctc cacgcccagt cgcgtaccgt
    12361 cttcatggga gaaaataata ctgttgatgg gtgtctggtc agagacatca agaaataacg
    12421 ccggaacatt agtgcaggca gcttccacag caatggcatc ctggtcatcc agcggatagt
    12481 taatgatcag cccactgacg cgttgcgcga gaagattgtg caccgccgct ttacaggctt
    12541 cgacgccgct tcgttctacc atcgacacca ccacgctggc acccagttga tcggcgcgag
    12601 atttaatcgc cgcgacaatt tgcgacggcg cgtgcagggc cagactggag gtggcaacgc
    12661 caatcagcaa cgactgtttg cccgccagtt gttgtgccac gcggttggga atgtaattca
    12721 gctccgccat cgccgcttcc actttttccc gcgttttcgc agaaacgtgg ctggcctggt
    12781 tcaccacgcg ggaaacggtc tgataagaga caccggcata ctctgcgaca tcgtataacg
    12841 ttactggttt cacattcacc accctgaatt gactctcttc cgggcgctat catgccatac
    12901 cgcgaaaggt tttgcgccat tcgatggtgt ccgggatctc gacgctctcc cttatgcgac
    12961 tcctgcatta ggaagcagcc cag
//