pRN114

LOCUS        pRN114               17232 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  BeYDV replicon with constitutive Luc expression, WUS2 and AtSTM.
ACCESSION   .
VERSION     .
KEYWORDS    pRN114
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17232)
  AUTHORS   Maher MF, Nasti RA, Vollbrecht M, Starker CG, Clark MD, Voytas DF
  TITLE     Plant gene editing through de novo induction of meristems.
  JOURNAL   Nat Biotechnol. 2019 Dec 16. pii: 10.1038/s41587-019-0337-2. doi: 
            10.1038/s41587-019-0337-2.
  PUBMED    31844292
REFERENCE   2  (bases 1 to 17232)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..17232
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..25
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    103..277
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(334..1131)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(445..464)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     1055..1074
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(1194..1870)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     protein_bind    2061..2082
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2097..2127
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2135..2151
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2140..2162
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2159..2175
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2159..2175
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        3080..3425
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             3443..5095
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(3509..3527)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     promoter        5499..5678
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        5531..5714
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     polyA_signal    9097..9271
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    10953..10977
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             12278..12907
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             13336..14409
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      14475..14669
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(14905..14927)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    15013..15153
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     15068..15087
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(15168..15185)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(15339..15927)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(15419..15438)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(16014..16808)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtactgaa ttaacgccga attaattcgg gggatctgga ttttagtact
      121 ggattttggt tttaggaatt agaaatttta ttgatagaag tattttacaa atacaaatac
      181 atactaaggg tttcttatat gctcaacaca tgagcgaaac cctataggaa ccctaattcc
      241 cttatctggg aactactcac acattattat ggagaaactc gagcttgtcg atcgactcta
      301 gctagaggat cgatccgaac cccagagtcc cgctcagaag aactcgtcaa gaaggcgata
      361 gaaggcgatg cgctgcgaat cgggagcggc gataccgtaa agcacgagga agcggtcagc
      421 ccattcgccg ccaagttctt cagcaatatc acgggtagcc aacgctatgt cctgatagcg
      481 gtccgccaca cccagccggc cacagtcgat gaatccagaa aagcggccat tttccaccat
      541 gatattcggc aagcaggcat cgccatgtgt cacgacgaga tcctcgccgt cgggcatgcg
      601 cgccttgagc ctggcgaaca gttcggctgg cgcgagcccc tgatgttctt cgtccagatc
      661 atcctgatcg acaagaccgg cttccatccg agtacgtgct cgctcgatgc gatgtttcgc
      721 ttggtggtcg aatgggcagg tagccggatc aagcgtatgc agccgccgca ttgcatcagc
      781 catgatggat actttctcgg caggagcaag gtgagatgac aggagatcct gccccggcac
      841 ttcgcccaat agcagccagt cccttcccgc ttcagtgaca acgtcgagca cagctgcgca
      901 aggaacgccc gtcgtggcca gccacgatag ccgcgctgcc tcgtcctgga gttcattcag
      961 ggcaccggac aggtcggtct tgacaaaaag aaccgggcgc ccctgcgctg acagccgaaa
     1021 cacggcggca tcagagcagc cgattgtctg ttgtgcccag tcatagccga atagcctctc
     1081 cacccaagcg gccggagaac ctgcgtgcaa tccatcttgt tcaatcccca tggtcgatcg
     1141 acagatctgc gaaagctcga gagagataga tttgtagaga gagactggtg atttcagcgt
     1201 gtcctctcca aatgaaatga acttccttat atagaggaag ggtcttgcga aggatagtgg
     1261 gattgtgcgt catcccttac gtcagtggag atatcacatc aatccacttg ctttgaagac
     1321 gtggttggaa cgtcttcttt ttccacgatg ctcctcgtgg gtgggggtcc atctttggga
     1381 ccactgtcgg cagaggcatc ttgaacgata gcctttcctt tatcgcaatg atggcatttg
     1441 taggtgccac cttccttttc tactgtcctt ttgatgaagt gacagatagc tgggcaatgg
     1501 aatccgagga ggtttcccga tattaccctt tgttgaaaag tctcaatagc cctttggtct
     1561 tctgagactg tatctttgat attcttggag tagacgagag tgtcgtgctc caccatgttc
     1621 acatcaatcc acttgctttg aagacgtggt tggaacgtct tctttttcca cgatgctcct
     1681 cgtgggtggg ggtccatctt tgggaccact gtcggcagag gcatcttgaa cgatagcctt
     1741 tcctttatcg caatgatggc atttgtaggt gccaccttcc ttttctactg tccttttgat
     1801 gaagtgacag atagctgggc aatggaatcc gaggaggttt cccgatatta ccctttgttg
     1861 aaaagtctca atagcccttt ggtcttctga gactgtatct ttgatattct tggagtagac
     1921 gagagtgtcg tgctccacca tgttggcaag ctgctctagc caatacgcaa accgcctctc
     1981 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     2041 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
     2101 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
     2161 ggaaacagct atgacatgat tacgaattcg agctcaaagt ttaacgcgtt agcagaaggc
     2221 atgttgttgt gactccgagg ggttgcctca aactctatct tataaccggc gtggaggcat
     2281 ggaggcaggg gtattttggt cattttaata gatagtggaa aatgacgtgg aatttactta
     2341 aagacgaagt ctttgcgaca agggggggcc cacgccgaat ttaatattac cggcgtggcc
     2401 cccccttatc gcgagtgctt tagcacgagc ggtccagatt taaagtagaa aatttcccgc
     2461 ccactagggt taaaggtgtt cacactataa aagcatatac gatgtgatgg tatttgatgg
     2521 agcgtatatt gtatcaggta tttccgttgg atacgaatta ttcgtacgac cctcggtacc
     2581 gatcggcgcg ccagatttgc cttttcaatt tcagaaagaa tgctaaccca cagatggtta
     2641 gagaggctta cgcagcaggt atcatcaaga cgatctaccc gagcaataat ctccaggaaa
     2701 tcaaatacct tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca
     2761 agaacacaga gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc
     2821 aaggcttgct tcacaaacca aggcaagtaa tagagattgg agtctctaaa aaggtagttc
     2881 ccactgaatc aaaggccatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg
     2941 taaagactgg cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct
     3001 tcgtcaacat ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct
     3061 cagaagacca aagggcaatt gagacttttc aacaaagggt aatatccgga aacctcctcg
     3121 gattccattg cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct
     3181 cctacaaatg ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca
     3241 gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa
     3301 ccacgtcttc aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac
     3361 aatcccacta tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga
     3421 gaacacgggg gactcctgca ggatggaaga cgccaaaaac ataaagaaag gcccggcgcc
     3481 attctatccg ctggaagatg gaaccgctgg agagcaactg cataaggcta tgaagagata
     3541 cgccctggtt cctggaacaa ttgcttttac agatgcacat atcgaggtgg acatcactta
     3601 cgctgagtac ttcgaaatgt ccgttcggtt ggcagaagct atgaaacgat atgggctgaa
     3661 tacaaatcac agaatcgtcg tatgcagtga aaactctctt caattcttta tgccggtgtt
     3721 gggcgcgtta tttatcggag ttgcagttgc gcccgcgaac gacatttata atgaacgtga
     3781 attgctcaac agtatgggca tttcgcagcc taccgtggtg ttcgtttcca aaaaggggtt
     3841 gcaaaaaatt ttgaacgtgc aaaaaaagct cccaatcatc caaaaaatta ttatcatgga
     3901 ttctaaaacg gattaccagg gatttcagtc gatgtacacg ttcgtcacat ctcatctacc
     3961 tcccggtttt aatgaatacg attttgtgcc agagtccttc gatagggaca agacaattgc
     4021 actgatcatg aactcctctg gatctactgg tctgcctaaa ggtgtcgctc tgcctcatag
     4081 aactgcctgc gtgagattct cgcatgccag agatcctatt tttggcaatc aaatcattcc
     4141 ggatactgcg attttaagtg ttgttccatt ccatcacggt tttggaatgt ttactacact
     4201 cggatatttg atatgtggat ttcgagtcgt cttaatgtat agatttgaag aagagctgtt
     4261 tctgaggagc cttcaggatt acaagattca aagtgcgctg ctggtgccaa ccctattctc
     4321 cttcttcgcc aaaagcactc tgattgacaa atacgattta tctaatttac acgaaattgc
     4381 ttctggtggc gctcccctct ctaaggaagt cggggaagcg gttgccaaga ggttccatct
     4441 gccaggtatc aggcaaggat atgggctcac tgagactaca tcagctattc tgattacacc
     4501 cgagggggat gataaaccgg gcgcggtcgg taaagttgtt ccattttttg aagcgaaggt
     4561 tgtggatctg gataccggga aaacgctggg cgttaatcaa agaggcgaac tgtgtgtgag
     4621 aggtcctatg attatgtccg gttatgtaaa caatccggaa gcgaccaacg ccttgattga
     4681 caaggatgga tggctacatt ctggagacat agcttactgg gacgaagacg aacacttctt
     4741 catcgttgac cgcctgaagt ctctgattaa gtacaaaggc tatcaggtgg ctcccgctga
     4801 attggaatcc atcttgctcc aacaccccaa catcttcgac gctggtgtcg caggtcttcc
     4861 cgacgatgac gccggtgaac ttcccgccgc cgttgttgtt ttggagcacg gaaagacgat
     4921 gacggaaaaa gagatcgtgg attacgtcgc cagtcaagta acaaccgcga aaaagttgcg
     4981 cggaggagtt gtgtttgtgg acgaagtacc gaaaggtctt accggaaaac tcgacgcaag
     5041 aaaaatcaga gagatcctca taaaggccaa gaagggcgga aagatcgccg tgtgacgtcg
     5101 acgatatgaa gatgaagatg aaatatttgg tgtgtcaaat aaaaagcttg tgtgcttaag
     5161 tttgtgtttt tttcttggct tgttgtgtta tgaatttgtg gctttttcta atattaaatg
     5221 aatgtaagat cacattataa tgaataaaca aatgtttcta taatccattg tgaatgtttt
     5281 gttggatctc ttctgcagca tataactact gtatgtgcta tggtatggac tatggaatat
     5341 gattaaagat aagccagagc tctggtgacg gacggcgcga ctagttttac gtacgttaat
     5401 taacccgggc gcgccgatca tgagcggaga attaagggag tcacgttatg acccccgccg
     5461 atgacgcggg acaagccgtt ttacgtttgg aactgacaga accgcaacgt tgaaggagcc
     5521 actcagccgc gggtttctgg agtttaatga gctaagcaca tacgtcagaa accattattg
     5581 cgcgttcaaa agtcgcctaa ggtcactatc agctagcaaa tatttcttgt caaaaatgct
     5641 ccactgacgt tccataaatt cccctcggta tccaattaga gtctcatatt cactctcaat
     5701 ccaaataatc tgcaccgtac ctgcagggtc cgagctaggt cacagaagcg ctcaggaagg
     5761 ccgctgagat agaggcatgg cggccaatgc gggcggcggt ggagcgggag gaggcagcgg
     5821 cagcggcagc gtggctgcgc cggcggtgtg ccgccccagc ggctcgcggt ggacgccgac
     5881 gccggagcag atcaggatgc tgaaggagct gtactacggc tgcggcatcc ggtcgcccag
     5941 ctcggagcag atccagcgca tcaccgccat gctgcggcag cacggcaaga tcgagggcaa
     6001 gaacgtcttc tactggttcc agaaccacaa ggcccgcgag cgccagaagc gccgcctcac
     6061 cagcctcgac gtgaacgtgc ccgccgccgg cgcggccgac gccaccacca gccaactcgg
     6121 cgtcctctcg ctgtcgtcgc cgccgccttc aggcgcggcg cctccctcgc ccaccctcgg
     6181 cttctacgcc gccggcaatg gcggcggatc ggctgtgctg ctggacacga gttccgactg
     6241 gggcagcagc ggcgctgcga tggccaccga gacatgcttc ctccaggact acatgggcgt
     6301 gacggacacg ggcagctcgt cgcagtggcc acgcttctcg tcgtcggaca cgataatggc
     6361 ggcggccgcg gcgcgggcgg cgacgacgcg ggcgcccgag actctccctc tcttcccgac
     6421 ctgcggcgac gacggcggca gcggtagcag cagctacttg ccgttctggg gtgccgcgtc
     6481 cacaactgcc ggcgccactt cttccgttgc gatccagcag caacaccagc tgcaggagca
     6541 gtacagcttt tacagcaaca gcaacagcac ccagctggcc ggcaccggca accaagacgt
     6601 atcggcaaca gcagcagcag ccgccgccct ggagctgagc ctcagctcat ggtgctcccc
     6661 ttaccctgct gcagggagta tgtgagagca acgcgagctg ccactgctct tcacttatgt
     6721 ctctggaatg gaaggaggag gaagtgagca tagcgttggt gcgttgctgt cattgtccta
     6781 ggttagtagc tagtgccagt tactagtaag catcaggcat aggagtatgt agtagaagca
     6841 tgcacgttgc cggccagcca ggctttagac gggaaaagaa tttggtgcag ccggctgcaa
     6901 aacaggatgt ttacagcccc cccctcgagc cctagacttg tccatcttct ggattggcca
     6961 agttaattaa tgtatgaaat aaaaggatgc acacatagtg acatgctaat cactataatg
     7021 tgggcatcaa agttgtgtgt tatgtgtaat tactaattat ctgaataaga gaaagagatc
     7081 atccatattt cttatcctaa atgaatgtca cgtgtcttta taattctttg atgaaccaga
     7141 tgcattttat taaccaattc catatacata taaatattaa tcatatataa ttaatatcaa
     7201 ttgggttagc aaaacaaatc tagtctaggt gtgttttgct aattattggg ggatagtgca
     7261 aaaagaaatc tacgttctca ataattcaga tagaaaactt aataaagtga gataatttac
     7321 atagattgct tttatccttt gatatatgtg aaaccatgca tgatataagg aaaatagata
     7381 gagaaataat tttttacatc gttgaatatg taaacaattt aattcaagaa gctaggaata
     7441 taaatattga ggagtttatg attagagctc tcccgctggc agacatactg tcccacaaat
     7501 gaagatggaa tctgtaaaag aaaacgcgtg aaataatgcg tctgacaaag gttaggtcgg
     7561 ctgcctttaa tcaataccaa agtggtccct accacgatgg aaaaactgtg cagtcggttt
     7621 ggctttttct gacgaacaaa taagattcgt ggccgacagg tgggggtcca ccatgtgaag
     7681 gcatcttcag actccaataa tggagcaatg acgtaagggc ttacgaaata agtaagggta
     7741 gtttgggaaa tgtccactca cccgtcagtc tataaatact tagcccctcc ctcattgtta
     7801 agggagcaaa atctcagaga gatagtccta gagagagaaa gagagcaagt agcctagaag
     7861 tagtcaaggc ggcgaagtat tcaggcacgt ggccaggaag aagaaaagcc aagacgacga
     7921 aaacaggtaa gagctaagct tatggagagt ggttccaaca gcacttcttg tccaatggct
     7981 tttgccgggg ataatagtga tggtccgatg tgtcctatga tgatgatgat gccgcccatc
     8041 atgacatcac atcaacatca tggtcatgat catcaacatc aacaacaaga acatgatggt
     8101 tatgcatatc agtcacacca ccaacaaagt agttcccttt ttcttcaatc actagctcct
     8161 ccccaaggaa ctaagaacaa agttgcttct tcttcttctc cttcctcttg tgctcctgcc
     8221 tattctctaa tggagatcca tcataacgaa atcgttgcag gaggaatcaa cccttgctcc
     8281 tcttcctctt cttcagcctc tgtcaaggcc aagatcatgg ctcatcctca ctaccaccgc
     8341 ctcttggccg cttatgtcaa ttgtcagaag gttggagcac caccggaggt tgtggcgagg
     8401 ctagaggagg catgctcgtc tgccgcagcc gctgccgcat ctatgggacc aacaggatgt
     8461 ctaggtgaag atccagggct tgatcaattc atggaagctt actgtgaaat gctcgttaag
     8521 tatgagcaag agctctccaa acctttcaag gaagctatgg tcttccttca acgtgtcgag
     8581 tgtcaattca aatccctctc tctatcctca ccttcctctt tctccggtta tggagagaca
     8641 gcaattgata ggaacaataa tgggtcatcc gaggaagaag tcgatatgaa caatgaattt
     8701 gtagatccac aagctgagga tagagagctt aaaggacagc tcttgcgcaa gtacagtggt
     8761 tacttaggga gcctcaagca agagttcatg aagaagagga agaaaggaaa gctccctaaa
     8821 gaagctcgtc aacaactgct tgattggtgg agccgtcact acaaatggcc ttacccttcg
     8881 gagcaacaaa agctcgccct tgcggaatca acggggctgg accagaaaca gataaacaat
     8941 tggttcataa accagaggaa acggcattgg aagccgtcgg aggacatgca gtttgtagta
     9001 atggacgcaa cacatcctca ccattacttc atggataatg tcttgggcaa tcctttccca
     9061 atggatcaca tctcctccac catgctttga ctcgagtttc tccataataa tgtgtgagta
     9121 gttcccagat aagggaatta gggttcctat agggtttcgc tcatgtgttg agcatataag
     9181 aaacccttag tatgtatttg tatttgtaaa atacttctat caataaaatt tctaattcct
     9241 aaaaccaaaa tccagtacta aaatccagat cccccgaatt aagtgtttga tcgccggcgg
     9301 taccgagtgt acttcaagtc agtgggaaat caataaaatg attattttat gaatatattt
     9361 cattgtgcaa gtagatagaa attacatatg ttacataaca cacgaaataa acaaaaaaag
     9421 acaatccaaa aacaaacacc ccaaaaaaaa taatcacttt agataaactc gtatgaggag
     9481 aggcacgttc agtgactcga cgattcccga gcaaaaaaag tctccccgtc acacatgtag
     9541 tgggtgacgc aattatcttt aaagtaatcc ttctgttgac ttgtcattga taacatccag
     9601 tcttcgtcag gattgcaaag aattatagaa gggatcccac cttttatttt cttctttttt
     9661 ccatatttag ggttgacagt gaaatcagac tggcaaccta ttaattgctt ccacaatggg
     9721 acgaacttga aggggatgtc gtcgatgata ttataggtgg cgtgttcatc gtagttggtg
     9781 aaatcgatgg taccgttcca atagttgtgt cgtccgagac ttctagccca ggtggtcttt
     9841 ccggtacgag ttggtccgca gatgtagagg ctggggtgtc ggattccatt ccttccattg
     9901 tccttgttaa atcggccatc cattcaaggt cagattgagc ttgttggtat gagacaggat
     9961 gtatgtaagt ataagcgtct atgcttacat ggtatagatg ggtttccctc caggagtgta
    10021 gatcttcgtg gcagcgaaga tctgattctg tgaagggcga cacatacggt tcaggttgtg
    10081 gagggaataa tttgttggct gaatattcca gccattgaag ctttgttgcc cattcatgag
    10141 ggaattcttc cttgatcatg tcaagatatt cctccttaga cgttgcagtc tggataatag
    10201 ttctccatcg tgcgtcagat ttgcgaggag aaaccttatg atctcggaaa tctcctctgg
    10261 ttttaatatc tccgtccttt gatatgtaat caaggacttg tttagagttt ctagctggct
    10321 ggatattagg gtgatttcct tcaaaatcga aaaaagaagg atccctaata caaggttttt
    10381 tatcaagctg gagaagagca tgatagtggg tagtgccatc ttgatgaagc tcagaagcaa
    10441 caccaaggaa gaaaataaga aaaggtgtga gtttctccca gagaaactgg aataaatcat
    10501 ctctttgaga tgagcacttg ggataggtaa ggaaaacata tttagattgg agtctgaagt
    10561 tcttactagc agaaggcatg ttgttgtgac tccgaggggt tgcctcaaac tctatcttat
    10621 aaccggcgtg gaggcatgga ggcaggggta ttttggtcat tttaatagat agtggaaaat
    10681 gacgtggaat ttacttaaag acgaagtctt tgcgacaagg gggggcccac gccgaattta
    10741 atattaccgg cgtggccccc ccttatcgcg agtgctttag cacgagcggt ccagatttaa
    10801 agtagaaaat ttcccgccca ctagggttaa aggtgttcac actataaaag catatacgat
    10861 gtgatggtat ttgatggagc gtatattgta tcaggtattt ccgttggata cgaattattc
    10921 gtacgaccct catagtttaa actatcagtg tttgacagga tatattggcg ggtaaaccta
    10981 agagaaaaga gcgtttatta gaataatcgg atatttaaaa gggcgtgaaa aggtttatcc
    11041 gttcgtccat ttgtatgtgc atgccaacca cagggttccc ctcgggatca aagtactttg
    11101 atccaacccc tccgctgcta tagtgcagtc ggcttctgac gttcagtgca gccgtcttct
    11161 gaaaacgaca tgtcgcacaa gtcctaagtt acgcgacagg ctgccgccct gcccttttcc
    11221 tggcgttttc ttgtcgcgtg ttttagtcgc ataaagtaga atacttgcga ctagaaccgg
    11281 agacattacg ccatgaacaa gagcgccgcc gctggcctgc tgggctatgc ccgcgtcagc
    11341 accgacgacc aggacttgac caaccaacgg gccgaactgc acgcggccgg ctgcaccaag
    11401 ctgttttccg agaagatcac cggcaccagg cgcgaccgcc cggagctggc caggatgctt
    11461 gaccacctac gccctggcga cgttgtgaca gtgaccaggc tagaccgcct ggcccgcagc
    11521 acccgcgacc tactggacat tgccgagcgc atccaggagg ccggcgcggg cctgcgtagc
    11581 ctggcagagc cgtgggccga caccaccacg ccggccggcc gcatggtgtt gaccgtgttc
    11641 gccggcattg ccgagttcga gcgttcccta atcatcgacc gcacccggag cgggcgcgag
    11701 gccgccaagg cccgaggcgt gaagtttggc ccccgcccta ccctcacccc ggcacagatc
    11761 gcgcacgccc gcgagctgat cgaccaggaa ggccgcaccg tgaaagaggc ggctgcactg
    11821 cttggcgtgc atcgctcgac cctgtaccgc gcacttgagc gcagcgagga agtgacgccc
    11881 accgaggcca ggcggcgcgg tgccttccgt gaggacgcat tgaccgaggc cgacgccctg
    11941 gcggccgccg agaatgaacg ccaagaggaa caagcatgaa accgcaccag gacggccagg
    12001 acgaaccgtt tttcattacc gaagagatcg aggcggagat gatcgcggcc gggtacgtgt
    12061 tcgagccgcc cgcgcacggc tcaaccgtgc ggctgcatga aatcctggcc ggtttgtctg
    12121 atgccaagct ggcggcctgg ccggccagct tggccgctga agaaaccgag cgccgccgtc
    12181 taaaaaggtg atgtgtattt gagtaaaaca gcttgcgtca tgcggtcgct gcgtatatga
    12241 tgcgatgagt aaataaacaa atacgcaagg ggaacgcatg aaggttatcg ctgtacttaa
    12301 ccagaaaggc gggtcaggca agacgaccat cgcaacccat ctagcccgcg ccctgcaact
    12361 cgccggggcc gatgttctgt tagtcgattc cgatccccag ggcagtgccc gcgattgggc
    12421 ggccgtgcgg gaagatcaac cgctaaccgt tgtcggcatc gaccgcccga cgattgaccg
    12481 cgacgtgaag gccatcggcc ggcgcgactt cgtagtgatc gacggagcgc cccaggcggc
    12541 ggacttggct gtgtccgcga tcaaggcagc cgacttcgtg ctgattccgg tgcagccaag
    12601 cccttacgac atatgggcca ccgccgacct ggtggagctg gttaagcagc gcattgaggt
    12661 cacggatgga aggctacaag cggcctttgt cgtgtcgcgg gcgatcaaag gcacgcgcat
    12721 cggcggtgag gttgccgagg cgctggccgg gtacgagctg cccattcttg agtcccgtat
    12781 cacgcagcgc gtgagctacc caggcactgc cgccgccggc acaaccgttc ttgaatcaga
    12841 acccgagggc gacgctgccc gcgaggtcca ggcgctggcc gctgaaatta aatcaaaact
    12901 catttgagtt aatgaggtaa agagaaaatg agcaaaagca caaacacgct aagtgccggc
    12961 cgtccgagcg cacgcagcag caaggctgca acgttggcca gcctggcaga cacgccagcc
    13021 atgaagcggg tcaactttca gttgccggcg gaggatcaca ccaagctgaa gatgtacgcg
    13081 gtacgccaag gcaagaccat taccgagctg ctatctgaat acatcgcgca gctaccagag
    13141 taaatgagca aatgaataaa tgagtagatg aattttagcg gctaaaggag gcggcatgga
    13201 aaatcaagaa caaccaggca ccgacgccgt ggaatgcccc atgtgtggag gaacgggcgg
    13261 ttggccaggc gtaagcggct gggttgcctg ccggccctgc aatggcactg gaacccccaa
    13321 gcccgaggaa tcggcgtgag cggtcgcaaa ccatccggcc cggtacaaat cggcgcggcg
    13381 ctgggtgatg acctggtgga gaagttgaag gccgcgcagg ccgcccagcg gcaacgcatc
    13441 gaggcagaag cacgccccgg tgaatcgtgg caagcggccg ctgatcgaat ccgcaaagaa
    13501 tcccggcaac cgccggcagc cggtgcgccg tcgattagga agccgcccaa gggcgacgag
    13561 caaccagatt ttttcgttcc gatgctctat gacgtgggca cccgcgatag tcgcagcatc
    13621 atggacgtgg ccgttttccg tctgtcgaag cgtgaccgac gagctggcga ggtgatccgc
    13681 tacgagcttc cagacgggca cgtagaggtt tccgcagggc cggccggcat ggccagtgtg
    13741 tgggattacg acctggtact gatggcggtt tcccatctaa ccgaatccat gaaccgatac
    13801 cgggaaggga agggagacaa gcccggccgc gtgttccgtc cacacgttgc ggacgtactc
    13861 aagttctgcc ggcgagccga tggcggaaag cagaaagacg acctggtaga aacctgcatt
    13921 cggttaaaca ccacgcacgt tgccatgcag cgtacgaaga aggccaagaa cggccgcctg
    13981 gtgacggtat ccgagggtga agccttgatt agccgctaca agatcgtaaa gagcgaaacc
    14041 gggcggccgg agtacatcga gatcgagcta gctgattgga tgtaccgcga gatcacagaa
    14101 ggcaagaacc cggacgtgct gacggttcac cccgattact ttttgatcga tcccggcatc
    14161 ggccgttttc tctaccgcct ggcacgccgc gccgcaggca aggcagaagc cagatggttg
    14221 ttcaagacga tctacgaacg cagtggcagc gccggagagt tcaagaagtt ctgtttcacc
    14281 gtgcgcaagc tgatcgggtc aaatgacctg ccggagtacg atttgaagga ggaggcgggg
    14341 caggctggcc cgatcctagt catgcgctac cgcaacctga tcgagggcga agcatccgcc
    14401 ggttcctaat gtacggagca gatgctaggg caaattgccc tagcagggga aaaaggtcga
    14461 aaaggcctct ttcctgtgga tagcacgtac attgggaacc caaagccgta cattgggaac
    14521 cggaacccgt acattgggaa cccaaagccg tacattggga accggtcaca catgtaagtg
    14581 actgatataa aagagaaaaa aggcgatttt tccgcctaaa actctttaaa acttattaaa
    14641 actcttaaaa cccgcctggc ctgtgcataa ctgtctggcc agcgcacagc ccaagagctg
    14701 caaaaagcgc ctacccttcg gtcgctgcgc tccctacgcc ccgccgcttc gcgtcggcct
    14761 atcgcggccg ctggccgctc aaaaatggct ggcctacggc caggcaatct accagggcgc
    14821 ggacaagccg cgccgtcgcc actcgaccgc cggcgcccac atcaaggcac cctgcctcgc
    14881 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaaa cggtcacagc
    14941 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
    15001 cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt atactggctt
    15061 aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg
    15121 cacagatgcg taaggagaaa ataccgcatc aggccctctt ccgcttcctc gctcactgac
    15181 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
    15241 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
    15301 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
    15361 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
    15421 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
    15481 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
    15541 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
    15601 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
    15661 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
    15721 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
    15781 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
    15841 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
    15901 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
    15961 gctcagtgga acgaaaactc acgttaaggg attttggtca tgcattctag gtactaaaac
    16021 aattcatcca gtaaaatata atattttatt ttctcccaat caggcttgat ccccagtaag
    16081 tcaaaaaata gctcgacata ctgttcttcc ccgatatcct ccctgatcga ccggacgcag
    16141 aaggcaatgt cataccactt gtccgccctg ccgcttctcc caagatcaat aaagccactt
    16201 actttgccat ctttcacaaa gatgttgctg tctcccaggt cgccgtggga aaagacaagt
    16261 tcctcttcgg gcttttccgt ctttaaaaaa tcatacagct cgcgcggatc tttaaatgga
    16321 gtgtcttctt cccagttttc gcaatccaca tcggccagat cgttattcag taagtaatcc
    16381 aattcggcta agcggctgtc taagctattc gtatagggac aatccgatat gtcgatggag
    16441 tgaaagagcc tgatgcactc cgcatacagc tcgataatct tttcagggct ttgttcatct
    16501 tcatactctt ccgagcaaag gacgccatcg gcctcactca tgagcagatt gctccagcca
    16561 tcatgccgtt caaagtgcag gacctttgga acaggcagct ttccttccag ccatagcatc
    16621 atgtcctttt cccgttccac atcataggtg gtccctttat accggctgtc cgtcattttt
    16681 aaatataggt tttcattttc tcccaccagc ttatatacct tagcaggaga cattccttcc
    16741 gtatctttta cgcagcggta tttttcgatc agttttttca attccggtga tattctcatt
    16801 ttagccattt attatttcct tcctcttttc tacagtattt aaagataccc caagaagcta
    16861 attataacaa gacgaactcc aattcactgt tccttgcatt ctaaaacctt aaataccaga
    16921 aaacagcttt ttcaaagttg ttttcaaagt tggcgtataa catagtatcg acggagccga
    16981 ttttgaaacc gcggtgatca caggcagcaa cgctctgtca tcgttacaat caacatgcta
    17041 ccctccgcga gatcatccgt gtttcaaacc cggcagctta gttgccgttc ttccgaatag
    17101 catcggtaac atgagcaaag tctgccgcct tacaacggct ctcccgctga cgccgtcccg
    17161 gactgatggg ctgcctgtat cgagtggtga ttttgtgccg agctgccggt cggggagctg
    17221 ttggctggct gg
//