| 质粒名称: | pAAV-CAG-AausFP1-P2A-FusionRed |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000280 |
| 产品用途: | Expresses AausFP1 and FusionRed in mammalian cells culture |
| 存储实验室: | KIRYL PIATKEVICH Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CAG |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a，TOP10 |
|
插入基因信息 |
|
| 插入基因名称: |
AausFP1, FusionRed
|
| 物种来源: | Synthetic, synthetic |
| 密码子优化: | 哺乳动物表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Bright and stable monomeric green fluorescent protein derived from StayGold
Zhang H., Lesnov G.D., Subach O.M., Zhang W., Kuzmicheva T.P., Vlaskina A.V., Samygina V.R., Chen L., Ye X., Nikolaeva A.Y., Gabdulkhakov A., Papadaki S., Qin W., Borshchevskiy V., Perfilov M.M., Gavrikov A.S., Drobizhev M., Mishin A.S., Piatkevich K.D., Subach F.V.
Nat. Methods 2024 214 21, 657–665 (2024)
|
pAAV-CAG-AausFP1-P2A-FusionRed
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAV-CAG-AausFP1-P2A-FusionRed载体质粒基本信息
pAAV-CAG-AausFP1-P2A-FusionRed质粒图谱载体图谱和pAAV-CAG-AausFP1-P2A-FusionRed载体序列质粒序列多克隆位点信息
pAAV-CAG-AausFP1-P2A-FusionRed质粒载体简介
pAAV-CAG-AausFP1-P2A-FusionRed质粒序列载体序列
LOCUS pAAV-CAG-AausFP1-P2A-FusionRed 6211 bp DNA circular SYN 01-NOV-2023
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6211)
AUTHORS Triple Threat
TITLE Direct Submission
JOURNAL Exported Jul 2, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6211
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 279..556
/label=chicken beta-actin promoter
primer_bind 951..1007
/label=0901-AAUSFP-F
misc_feature 977..982
/label=Kozak sequence
CDS 983..1678
/codon_start=1
/label=AausFP1
/translation="MSYGALLFREKIPYVVEMEGDVEGMKFSVRGKGHGDANTGKIEAS
FICTTGELPVPWSSILTTVTYGAQCFAKYPNDIKDYPKSAMPEGYVQERTITFENDGVY
KTRAEVTYEKGSVYNRVTLNGSGFKKGGNILGKKLEFNYNPHCIYVLPDVQNNGIKCYI
NIVHDVIGGGQIIAAHQQLNTPLGGGPVDIPHYHHIQAHTILSKDPKETRDHMNVVEVF
RAIDCKTAYA"
primer_bind complement(1657..1711)
/label=0901-AAUSFP-R
CDS 1703..1759
/codon_start=1
/product="2A peptide from porcine teschovirus-1
polyprotein"
/label=P2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="ATNFSLLKQAGDVEENPGP"
CDS 1772..2467
/codon_start=1
/product="monomeric red fluorescent protein optimized for
use as a fusion tag (Shemiakina et al., 2012)"
/label=FusionRed
/note="mammalian codon-optimized"
/translation="MVSELIKENMPMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
VVEGGPLPFAFDILATSFMYGSRTFIKHPPGIPDFFKQSFPEGFTWERVTTYEDGGVLT
ATQDTSLQDGCLIYNVKVRGVNFPANGPVMQKKTLGWEASTETMYPADGGLEGACDMAL
KLVGGGHLICNLETTYRSKKPATNLKMPGVYNVDHRLERIKEADDETYVEQHEVAVARY
STGGAGDGGK"
primer_bind complement(2447..2491)
/label=0714-FusionRed-R
misc_feature 2495..3083
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
CDS complement(2966..2977)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
polyA_signal 3126..3247
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
repeat_region 3409..3538
/label=AAV2 ITR
rep_origin complement(3775..4363)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(4534..5394)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(5395..5499)
/gene="bla"
/label=AmpR promoter
rep_origin complement(5525..5980)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
repeat_region 6052..6181
/label=AAV2 ITR
ORIGIN
1 tctacgtagc catgctctag gaagagtacc attgacgtca ataatgacgt atgttcccat
61 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
121 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
181 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
241 gcagtacatc tacgtattag tcatcgctat taccatggtc gaggtgagcc ccacgttctg
301 cttcactctc cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta
361 attattttgt gcagcgatgg gggcgggggg gggggggggg cgcgcgccag gcggggcggg
421 gcggggcgag gggcggggcg gggcgaggcg gagaggtgcg gcggcagcca atcagagcgg
481 cgcgctccga aagtttcctt ttatggcgag gcggcggcgg cggcggccct ataaaaagcg
541 aagcgcgcgg cgggcgggag tcgctgcgcg ctgccttcgc cccgtgcccc gctccgccgc
601 cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta ctcccacagg tgagcgggcg
661 ggacggccct tctcctccgg gctgtaatta gcgcttggtt taatgacggc ttgtttcttt
721 tctgtggctg cgtgaaagcc ttgaggggct ccgggagggc cctttgtgcg gggggagcgg
781 ctcggggctg tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg
841 gcttctggcg tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct
901 ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat tttggcaaag
961 aattggatcc ggtaccgcca ccatgagtta cggagcactt ttgttcagag aaaaaatccc
1021 ctatgttgtt gagatggaag gtgatgttga gggaatgaag ttttcagtaa gaggtaaagg
1081 acatggtgat gctaacactg gtaaaattga agccagtttc atctgcacaa ctggtgaact
1141 accagtacct tggtcaagca ttctaacaac tgtcacatac ggtgcacagt gttttgcaaa
1201 atatccaaat gatatcaaag actacccaaa aagtgctatg ccagaaggtt acgttcaaga
1261 gcgaacaatc acttttgaaa acgacggcgt ttataagaca cgcgctgaag tcacgtacga
1321 gaaaggatca gtttataacc gagttacgtt gaacggttca ggatttaaaa aaggcggtaa
1381 catcttgggc aaaaaattgg aatttaacta caaccctcat tgcatctatg ttcttccaga
1441 tgtacaaaat aatggaatca aatgctacat caacattgtt catgacgtaa ttggaggagg
1501 acaaataatt gctgcgcacc aacaattaaa tactcctctt ggcggtggac cagtcgacat
1561 tccacactac catcatatac aagcgcacac aatactttct aaagatccca aagaaacaag
1621 agatcatatg aacgtagtag aggtgttcag agctatcgat tgcaaaaccg catatgcacg
1681 accggttcac gtgggaagtg gggctacaaa cttctcactg ctgaaacagg ccggggatgt
1741 ggaggaaaac ccaggaccac acgtgactag tatggtgagc gagctgatta aggagaacat
1801 gcccatgaag ctgtacatgg agggcaccgt gaacaaccac cacttcaagt gcacatccga
1861 gggcgaaggc aagccctacg agggcaccca gaccatgaga atcaaggtcg tcgagggcgg
1921 ccctctcccc ttcgccttcg acatcctggc taccagcttc atgtacggca gcagaacctt
1981 catcaagcac cctccgggca tccccgactt ctttaagcag tccttccctg agggcttcac
2041 atgggagaga gtcaccacat acgaagacgg gggcgtgctg accgctaccc aggacaccag
2101 cctccaggac ggctgcctca tctacaacgt caaggttaga ggggtgaact tcccagccaa
2161 cggccctgtg atgcagaaga aaacactcgg ctgggaggcc tccaccgaga cgatgtaccc
2221 tgctgacgga ggactggaag gcgcatgtga catggctctg aagctcgtgg gaggaggaca
2281 cctgatctgc aaccttgaga caacatacag atccaagaaa cccgctacga acctcaagat
2341 gcccggcgtc tacaacgtgg accacagact ggaaagaatc aaggaggccg acgatgagac
2401 atacgtcgag cagcacgagg tggctgtggc cagatactct actggtggcg ctggtgatgg
2461 aggtaaataa gaattcgata tcaagcttat cgataatcaa cctctggatt acaaaatttg
2521 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
2581 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
2641 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
2701 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
2761 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
2821 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
2881 gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg
2941 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
3001 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
3061 ctccctttgg gccgcctccc cgcatcgata ccgtcgaccc gggcggccgc ttcgagcaga
3121 catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg
3181 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa
3241 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg agatgtggga
3301 ggttttttaa agcaagtaaa acctctacaa atgtggtaaa atcgataagg atcttcctag
3361 agcatggcta cgtagataag tagcatggcg ggttaatcat taactacaag gaacccctag
3421 tgatggagtt ggccactccc tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa
3481 aggtcgcccg acgcccgggc tttgcccggg cggcctcagt gagcgagcga gcgcgcagct
3541 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
3601 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
3661 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
3721 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
3781 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
3841 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
3901 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
3961 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
4021 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
4081 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
4141 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
4201 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
4261 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
4321 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
4381 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
4441 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
4501 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
4561 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
4621 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
4681 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
4741 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
4801 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
4861 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
4921 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
4981 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
5041 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
5101 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
5161 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
5221 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
5281 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
5341 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
5401 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
5461 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
5521 acctaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc
5581 tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc
5641 gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac
5701 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca
5761 ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg
5821 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag
5881 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc
5941 accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt cccattcgcc attcaggctg
6001 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctgcgcgct
6061 cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc gggcgacctt tggtcgcccg
6121 gcctcagtga gcgagcgagc gcgcagagag ggagtggcca actccatcac taggggttcc
6181 ttgtagttaa tgattaaccc gccatgctac t
//
pAAV-CAG-AausFP1-P2A-FusionRed载体图谱质粒图谱pdf版和pAAV-CAG-AausFP1-P2A-FusionRed载体序列质粒序列等相关资料下载
pAAV-CAG-AausFP1-P2A-FusionRed质粒载体应用举例
- 上一篇：pAAV-CAG-td8ox2StayGold(c4)-P2A-FusionRed
- 下一篇：没有了
