PABE-3

LOCUS       PABE-3               10342 bp ds-DNA     circular SYN 10-JAN-2019
DEFINITION  Targeted A to G in wheat plants or monocotyledons protoplasts .
ACCESSION   .
VERSION     .
KEYWORDS    PABE-3
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10342)
  AUTHORS   Li C, Zong Y, Wang Y, Jin S, Zhang D, Song Q, Zhang R, Gao C
  TITLE     Expanded base editing in rice and wheat using a Cas9-adenosine 
            deaminase fusion.
  JOURNAL   Genome Biol. 2018 May 29;19(1):59. doi: 10.1186/s13059-018-1443-z.
  PUBMED    29807545
REFERENCE   2  (bases 1 to 10342)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 10, 2019 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10342
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             2212..2232
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2245..6345
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     RBS             6258..6266
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             6346..6393
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     polyA_signal    6451..6627
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     primer_bind     complement(7181..7197)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7181..7197)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7194..7216)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7205..7221
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7229..7259)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7274..7295
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7412..7429)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7583..8171)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7663..7682)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8342..9202)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8965..8984
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9203..9307)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccgctcgtcc tccccccccc cccctctcta ccttctctag atcggcgttc cggtccatgg
       61 ttagggcccg gtagttctac ttctgttcat gtttgtgtta gatccgtgtt tgtgttagat
      121 ccgtgctgct agcgttcgta cacggatgcg acctgtacgt cagacacgtt ctgattgcta
      181 acttgccagt gtttctcttt ggggaatcct gggatggctc tagccgttcc gcagacggga
      241 tcgatttcat gatttttttt gtttcgttgc atagggtttg gtttgccctt ttcctttatt
      301 tcaatatatg ccgtgcactt gtttgtcggg tcatcttttc atgctttttt ttgtcttggt
      361 tgtgatgatg tggtctggtt gggcggtcgt tctagatcgg agtagaatta attctgtttc
      421 aaactacctg gtggatttat taattttgga tctgtatgtg tgtgccatac atattcatag
      481 ttacgaattg aagatgatgg atggaaatat cgatctagga taggtataca tgttgatgcg
      541 ggttttactg atgcatatac agagatgctt tttgttcgct tggttgtgat gatgtggtgt
      601 ggttgggcgg tcgttcattc gttctagatc ggagtagaat actgtttcaa actacctggt
      661 gtatttatta attttggaac tgtatgtgtg tgtcatacat cttcatagtt acgagtttaa
      721 gatggatgga aatatcgatc taggataggt atacatgttg atgtgggttt tactgatgca
      781 tatacatgat ggcatatgca gcatctattc atatgctcta accttgagta cctatctatt
      841 ataataaaca agtatgtttt ataattattt tgatcttgat atacttggat gatggcatat
      901 gcagcagcta tatgtggatt tttttagccc tgccttcata cgctatttat ttgcttggta
      961 ctgtttcttt tgtcgatgct caccctgttg tttggtgtta cttctgcaaa gcttatgtcc
     1021 gaggtggagt tctctcacga gtattggatg aggcacgctc ttacacttgc taagagagct
     1081 tgggacgaaa gagaagtgcc agttggcgcc gttcttgtgc ataataatag ggtgatcggc
     1141 gagggttgga atagaccaat tggaaggcat gatccaacag ctcacgcaga gattatggct
     1201 ctcagacaag gcggcctcgt tatgcagaac tacaggctca ttgacgctac actctacgtg
     1261 acactcgaac cttgcgttat gtgcgccgga gctatgattc attctaggat tggcagggtc
     1321 gtgtttggag ctagggacgc taaaacagga gccgccggat ctcttatgga cgtgttgcat
     1381 catccaggca tgaaccatag ggtggagatt acagagggca ttcttgcaga cgagtgcgct
     1441 gctcttcttt ccgatttctt caggatgaga aggcaggaga ttaaggccca gaagaaggct
     1501 cagtcttcta cagatagcgg aggatcttcc ggaggatcta gcggctccga gacaccagga
     1561 acatccgaaa gcgctacacc agaatctagc ggaggctctt ccggaggatc ttctgaagtg
     1621 gagttctccc acgagtattg gatgaggcac gctcttacac ttgctaaaag ggctagggac
     1681 gaaagggaag ttccagttgg agctgttctc gtgctcaata acagggtgat tggcgagggt
     1741 tggaatagag ccattggact ccatgatcca acagctcacg cagagattat ggctcttaga
     1801 caaggcggcc tcgttatgca gaattacaga ctcatcgacg ccacactcta cgttaccttc
     1861 gaaccttgcg ttatgtgcgc cggagctatg atccattcta ggattggcag ggtcgtgttc
     1921 ggcgttagaa acgctaagac aggagctgca ggctctctta tggacgttct tcattaccca
     1981 ggcatgaatc atagagtgga gatcacagaa ggcattcttg cagacgagtg cgcagctctc
     2041 ctttgctatt tcttcaggat gccgaggcaa gttttcaacg ctcagaagaa ggcccagtct
     2101 tctacagatt ccggcggatc ttctggagga tctagcggct ccgagacacc aggaacatcc
     2161 gaatccgcta caccagagtc ttctggagga tctagcggag gatctcttaa gccaaaaaag
     2221 aagagaaagg tttcaggcgg ctccgacaag aagtactcga tcggcctcgc cattgggact
     2281 aactctgttg gctgggccgt gatcaccgac gagtacaagg tgccctcaaa gaagttcaag
     2341 gtcctgggca acaccgatcg gcattccatc aagaagaatc tcattggcgc tctcctgttc
     2401 gacagcggcg agacggctga ggctacgcgg ctcaagcgca ccgcccgcag gcggtacacg
     2461 cgcaggaaga atcgcatctg ctacctgcag gagattttct ccaacgagat ggcgaaggtt
     2521 gacgattctt tcttccacag gctggaggag tcattcctcg tggaggagga taagaagcac
     2581 gagcggcatc caatcttcgg caacattgtc gacgaggttg cctaccacga gaagtaccct
     2641 acgatctacc atctgcggaa gaagctcgtg gactccacag ataaggcgga cctccgcctg
     2701 atctacctcg ctctggccca catgattaag ttcaggggcc atttcctgat cgagggggat
     2761 ctcaacccgg acaatagcga tgttgacaag ctgttcatcc agctcgtgca gacgtacaac
     2821 cagctcttcg aggagaaccc cattaatgcg tcaggcgtcg acgcgaaggc tatcctgtcc
     2881 gctaggctct cgaagtctcg gcgcctcgag aacctgatcg cccagctgcc gggcgagaag
     2941 aagaacggcc tgttcgggaa tctcattgcg ctcagcctgg ggctcacgcc caacttcaag
     3001 tcgaatttcg atctcgctga ggacgccaag ctgcagctct ccaaggacac atacgacgat
     3061 gacctggata acctcctggc ccagatcggc gatcagtacg cggacctgtt cctcgctgcc
     3121 aagaatctgt cggacgccat cctcctgtct gatattctca gggtgaacac cgagattacg
     3181 aaggctccgc tctcagcctc catgatcaag cgctacgacg agcaccatca ggatctgacc
     3241 ctcctgaagg cgctggtcag gcagcagctc cccgagaagt acaaggagat cttcttcgat
     3301 cagtcgaaga acggctacgc tgggtacatt gacggcgggg cctctcagga ggagttctac
     3361 aagttcatca agccgattct ggagaagatg gacggcacgg aggagctgct ggtgaagctc
     3421 aatcgcgagg acctcctgag gaagcagcgg acattcgata acggcagcat cccacaccag
     3481 attcatctcg gggagctgca cgctatcctg aggaggcagg aggacttcta ccctttcctc
     3541 aaggataacc gcgagaagat cgagaagatt ctgactttca ggatcccgta ctacgtcggc
     3601 ccactcgcta ggggcaactc ccgcttcgct tggatgaccc gcaagtcaga ggagacgatc
     3661 acgccgtgga acttcgagga ggtggtcgac aagggcgcta gcgctcagtc gttcatcgag
     3721 aggatgacga atttcgacaa gaacctgcca aatgagaagg tgctccctaa gcactcgctc
     3781 ctgtacgagt acttcacagt ctacaacgag ctgactaagg tgaagtatgt gaccgagggc
     3841 atgaggaagc cggctttcct gtctggggag cagaagaagg ccatcgtgga cctcctgttc
     3901 aagaccaacc ggaaggtcac ggttaagcag ctcaaggagg actacttcaa gaagattgag
     3961 tgcttcgatt cggtcgagat ctctggcgtt gaggaccgct tcaacgcctc cctggggacc
     4021 taccacgatc tcctgaagat cattaaggat aaggacttcc tggacaacga ggagaatgag
     4081 gatatcctcg aggacattgt gctgacactc actctgttcg aggaccggga gatgatcgag
     4141 gagcgcctga agacttacgc ccatctcttc gatgacaagg tcatgaagca gctcaagagg
     4201 aggaggtaca ccggctgggg gaggctgagc aggaagctca tcaacggcat tcgggacaag
     4261 cagtccggga agacgatcct cgacttcctg aagagcgatg gcttcgcgaa ccgcaatttc
     4321 atgcagctga ttcacgatga cagcctcaca ttcaaggagg atatccagaa ggctcaggtg
     4381 agcggccagg gggactcgct gcacgagcat atcgcgaacc tcgctggctc gccagctatc
     4441 aagaagggga ttctgcagac cgtgaaggtt gtggacgagc tggtgaaggt catgggcagg
     4501 cacaagcctg agaacatcgt cattgagatg gcccgggaga atcagaccac gcagaagggc
     4561 cagaagaact cacgcgagag gatgaagagg atcgaggagg gcattaagga gctggggtcc
     4621 cagatcctca aggagcaccc ggtggagaac acgcagctgc agaatgagaa gctctacctg
     4681 tactacctcc agaatggccg cgatatgtat gtggaccagg agctggatat taacaggctc
     4741 agcgattacg acgtcgatca tatcgttcca cagtcattcc tgaaggatga ctccattgac
     4801 aacaaggtcc tcaccaggtc ggacaagaac cggggcaagt ctgataatgt tccttcagag
     4861 gaggtcgtta agaagatgaa gaactactgg cgccagctcc tgaatgccaa gctgatcacg
     4921 cagcggaagt tcgataacct cacaaaggct gagaggggcg ggctctctga gctggacaag
     4981 gcgggcttca tcaagaggca gctggtcgag acacggcaga tcactaagca cgttgcgcag
     5041 attctcgact cacggatgaa cactaagtac gatgagaatg acaagctgat ccgcgaggtg
     5101 aaggtcatca ccctgaagtc aaagctcgtc tccgacttca ggaaggattt ccagttctac
     5161 aaggttcggg agatcaacaa ttaccaccat gcccatgacg cgtacctgaa cgcggtggtc
     5221 ggcacagctc tgatcaagaa gtacccaaag ctcgagagcg agttcgtgta cggggactac
     5281 aaggtttacg atgtgaggaa gatgatcgcc aagtcggagc aggagattgg caaggctacc
     5341 gccaagtact tcttctactc taacattatg aatttcttca agacagagat cactctggcc
     5401 aatggcgaga tccggaagcg ccccctcatc gagacgaacg gcgagacggg ggagatcgtg
     5461 tgggacaagg gcagggattt cgcgaccgtc aggaaggttc tctccatgcc acaagtgaat
     5521 atcgtcaaga agacagaggt ccagactggc gggttctcta aggagtcaat tctgcctaag
     5581 cggaacagcg acaagctcat cgcccgcaag aaggactggg atccgaagaa gtacggcggg
     5641 ttcgacagcc ccactgtggc ctactcggtc ctggttgtgg cgaaggttga gaagggcaag
     5701 tccaagaagc tcaagagcgt gaaggagctg ctggggatca cgattatgga gcgctccagc
     5761 ttcgagaaga acccgatcga tttcctggag gcgaagggct acaaggaggt gaagaaggac
     5821 ctgatcatta agctccccaa gtactcactc ttcgagctgg agaacggcag gaagcggatg
     5881 ctggcttccg ctggcgagct gcagaagggg aacgagctgg ctctgccgtc caagtatgtg
     5941 aacttcctct acctggcctc ccactacgag aagctcaagg gcagccccga ggacaacgag
     6001 cagaagcagc tgttcgtcga gcagcacaag cattacctcg acgagatcat tgagcagatt
     6061 tccgagttct ccaagcgcgt gatcctggcc gacgcgaatc tggataaggt cctctccgcg
     6121 tacaacaagc accgcgacaa gccaatcagg gagcaggctg agaatatcat tcatctcttc
     6181 accctgacga acctcggcgc ccctgctgct ttcaagtact tcgacacaac tatcgatcgc
     6241 aagaggtaca caagcactaa ggaggtcctg gacgcgaccc tcatccacca gtcgattacc
     6301 ggcctctacg agacgcgcat cgacctgtct cagctcgggg gcgacaagcg gccagcggcg
     6361 acgaagaagg cggggcaggc gaagaagaag aagtaggaat tcggtacgct gaaatcacca
     6421 gtctctctct acaaatctat ctctctctat tttctccata aataatgtgt gagtagtttc
     6481 ccgataaggg aaattagggt tcttataggg tttcgctcat gtgttgagca tataagaaac
     6541 ccttagtatg tatttgtatt tgtaaaatac ttctatcaat aaaatttcta attcctaaaa
     6601 ccaaaatcca gtactaaaat ccagatctcc taaagtccct atagatcttt gtcgtgaata
     6661 taaaccagac acgagacgac taaacctgga gcccagacgc cgttcgaagc tagaagtacc
     6721 gcttaggcag gaggccgtta gggaaaagat gctaaggcag ggttggttac gttgactccc
     6781 ccgtaggttt ggtttaaata tgatgaagtg gacggaagga aggaggaaga caaggaagga
     6841 taaggttgca ggccctgtgc aaggtaagaa gatggaaatt tgatagaggt acgctactat
     6901 acttatacta tacgctaagg gaatgcttgt atttataccc tataccccct aataacccct
     6961 tatcaattta agaaataatc cgcataagcc cccgcttaaa aattggtatc agagccatga
     7021 ataggtctat gaccaaaact caagaggata aaacctcacc aaaatacgaa agagttctta
     7081 actctaaaga taaaagatct ttcaagatca aaactagttc cctcacaccg gtgacgggga
     7141 tcgcatgcga tatctcgaga tctagcttgg cgtaatcatg gtcatagctg tttcctgtgt
     7201 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     7261 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     7321 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     7381 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     7441 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     7501 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     7561 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     7621 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     7681 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     7741 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     7801 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     7861 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     7921 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     7981 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     8041 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     8101 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     8161 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     8221 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     8281 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     8341 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     8401 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     8461 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     8521 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     8581 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     8641 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     8701 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     8761 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     8821 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     8881 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     8941 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     9001 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     9061 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     9121 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     9181 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     9241 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     9301 ttccgcgcac atttccccga aaagtgccac ctgccagtgc caagctaatt cgagctcggt
     9361 acctgacccg gtcgtgcccc tctctagaga taatgagcat tgcatgtcta agttataaaa
     9421 aattaccaca tatttttttt gtcacacttg tttgaagtgc agtttatcta tctttataca
     9481 tatatttaaa ctttactcta cgaataatat aatctatagt actacaataa tatcagtgtt
     9541 ttagagaatc atataaatga acagttagac atggtctaaa ggacaattga gtattttgac
     9601 aacaggactc tacagtttta tctttttagt gtgcatgtgt tctccttttt ttttgcaaat
     9661 agcttcacct atataatact tcatccattt tattagtaca tccatttagg gtttagggtt
     9721 aatggttttt atagactaat ttttttagta catctatttt attctatttt agcctctaaa
     9781 ttaagaaaac taaaactcta ttttagtttt tttatttaat aatttagata taaaatagaa
     9841 taaaataaag tgactaaaaa ttaaacaaat accctttaag aaattaaaaa aactaaggaa
     9901 acatttttct tgtttcgagt agataatgcc agcctgttaa acgccgtcga cgagtctaac
     9961 ggacaccaac cagcgaacca gcagcgtcgc gtcgggccaa gcgaagcaga cggcacggca
    10021 tctctgtcgc tgcctctgga cccctctcga tcgagagttc cgctccaccg ttggacttgc
    10081 tccgctgtcg gcatccagaa attgcgtggc ggagcggcag acgtgagccg gcacggcagg
    10141 cggcctcctc ctcctctcac ggcaccggca gctacggggg attcctttcc caccgctcct
    10201 tcgctttccc ttcctcgccc gccgtaataa atagacaccc cctccacacc ctctttcccc
    10261 aacctcgtgt tgttcggagc gcacacacac acaaccagat ctcccccaaa tccacccgtc
    10321 ggcacctccg cttcaaggta cg
//