| 质粒名称: | pWZ853 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 208115 |
| 存储实验室: | Jef Boeke |
|
载体骨架基本信息 |
|
| 载体名称: | TOPO |
| 载体出品公司: | Invitrogen |
| 空载体大小: | 3500 bp |
| 完整质粒大小: | 7616bp |
| 载体类型: | Mammalian Expression, Bacterial Expression |
| 筛选标记: | Blasticidin |
| 载体抗性: | Kanamycin, 50 μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | NeonGreen-HPRT1-BSD、Marker cassette 2.2 |
| 物种来源: | H. sapiens (human), M. musculus (mouse) |
| 插入大小: | 4100bp |
| 基因突变: | T2A, S13L, A92T, V104T, V114I, E161K, Y193K, F198Y, D202T, I203V, Y258F, A283V, K288T, N290Y relative to RpBphP2 |
| GenBank编号 | NM_000194.3 |
| 启动子: | EF1alpha |
| 标签/融合蛋白: | T2A (C terminal on insert) |
| 克隆方法: | Gibson Cloning |
| 5’克隆位点: | AgeI(未破坏) |
| 3’克隆位点: | NotI (未破坏) |
| 5’测序引物 | M13F |
| 3’测序引物 | M13R |
|
文献引用方法 |
|
| 材料和方法部分: | pWZ853 was a gift from Jef Boeke (Addgene plasmid # 208115 ; http://n2t.net/addgene:208115 ; RRID:Addgene_208115) |
| 参考文献部分: | Mouse genome rewriting and tailoring of three important disease loci. Zhang W, Golynker I, Brosh R, Fajardo A, Zhu Y, Wudzinska AM, Ordonez R, Ribeiro-Dos-Santos AM, Carrau L, Damani-Yokota P, Yeung ST, Khairallah C, Vela Gartner A, Chalhoub N, Huang E, Ashe HJ, Khanna KM, Maurano MT, Kim SY, tenOever BR, Boeke JD. Nature. 2023 Nov 1. doi: 10.1038/s41586-023-06675-4. 10.1038/s41586-023-06675-4 PubMed 37914927 |
买家导航
pWZ853载体质粒基本信息
pWZ853质粒图谱载体图谱和pWZ853载体序列质粒序列多克隆位点信息
pWZ853质粒载体简介
pWZ853质粒序列载体序列
LOCUS pWZ853 7616 bp DNA circular SYN 25-AUG-2024
DEFINITION mSwAP-In marker cassette 2 with 5'PB-mNeongreen-HPRT1-BSD.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7616)
AUTHORS Zhang W, Golynker I, Brosh R, Fajardo A, Zhu Y, Wudzinska AM,
Ordonez R, Ribeiro-Dos-Santos AM, Carrau L, Damani-Yokota P, Yeung
ST, Khairallah C, Vela Gartner A, Chalhoub N, Huang E, Ashe HJ,
Khanna KM, Maurano MT, Kim SY, tenOever BR, Boeke JD
TITLE Mouse genome rewriting and tailoring of three important disease
loci.
JOURNAL Nature. 2023 Nov 1. doi: 10.1038/s41586-023-06675-4.
PUBMED 37914927
REFERENCE 2 (bases 1 to 7616)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 25, 2024 from SnapGene Server 7.0.3
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Nature.
2023 Nov 1. doi: 10.1038/s41586-023-06675-4."
FEATURES Location/Qualifiers
source 1..7616
/mol_type="other DNA"
/organism="synthetic DNA construct"
protein_bind 107..128
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 143..173
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 181..197
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 186..208
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 205..221
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 205..221
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
promoter 239..257
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind 239..256
/label=SP6
/note="SP6 promoter, forward primer"
repeat_region 341..375
/label=piggyBac left (5') inverted repeat
/note="piggyBac transposon-specific inverted terminal
repeat sequence (ITR)"
promoter 735..1913
/label=EF-1-alpha promoter
/note="strong constitutive promoter for human elongation
factor EF-1-alpha"
intron 966..1904
/label=EF-1-alpha intron A
/note="intron upstream of the start codon of human
EF-1-alpha"
primer_bind 1861..1881
/label=EF1a-F
/note="Human elongation factor-1a promoter, forward primer"
regulatory 1934..1943
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 1940..2647
/codon_start=1
/product="bright monomeric yellow-green fluorescent protein
derived from LanYFP (Shaner et al., 2013)"
/label=mNeonGreen
/translation="MVSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYE
ELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFED
GASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIS
TFKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQ
KAFTDVMGMDELYK"
regulatory 2195..2204
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
regulatory 2678..2687
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 3347..3400
/codon_start=1
/product="2A peptide from Thosea asigna virus capsid
protein"
/label=T2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="EGRGSLLTCGDVEENPGP"
CDS 3434..3829
/codon_start=1
/gene="Aspergillus terreus BSD"
/product="blasticidin S deaminase"
/label=BSD
/note="confers resistance to blasticidin"
/translation="AKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
AIVKDSDGQPTAVGIRELLPSGYVWEG"
polyA_signal 3987..4108
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(4024..4043)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
repeat_region 4371..4433
/label=piggyBac right (3') inverted repeat
/note="piggyBac transposon-specific inverted terminal
repeat sequence (ITR)"
primer_bind complement(4504..4523)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(4505..4523)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(4530..4547)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(4530..4546)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(4539..4561)
/label=M13/pUC Forward
/note="In lacZ gene"
CDS 4684..4986
/codon_start=1
/gene="ccdB"
/product="CcdB, a bacterial toxin that poisons DNA gyrase"
/label=ccdB
/note="Plasmids containing the ccdB gene cannot be
propagated in standard E. coli strains."
/translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
primer_bind 4865..4884
/label=ccdB-fwd
/note="ccdB gene, forward primer"
CDS 5335..6129
/codon_start=1
/product="aminoglycoside phosphotransferase"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
CDS 5335..6129
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(5389..5408)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 5999..6018
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
regulatory 6329..6338
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 6335..6709
/codon_start=1
/gene="Sh ble from Streptoalloteichus hindustanus"
/product="antibiotic-binding protein"
/label=BleoR
/note="confers resistance to bleomycin, phleomycin, and
Zeocin(TM)"
/translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
EFALRDPAGNCVHFVAEEQD"
rep_origin 6847..7435
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 7336..7355
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 7589..7606
/label=L4440
/note="L4440 vector, forward primer"
ORIGIN
1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctat
241 ttaggtgaca ctatagaata ctcaagctat gcatcaagct tggtaccgag ctcggatcca
301 ctagtaacgg ccgccagtgt gctggaattc gcccttttaa ccctagaaag atagtctgcg
361 taaaattgac gcatgcattc ttgaaatatt gctctctctt tctaaatagc gcgaatccgt
421 cgctgtgcat ttaggacatc tcagtcgccg cttggagctc ccgtgaggcg tgcttgtcaa
481 tgcggtaagt gtcactgatt ttgaactata acgaccgcgt gagtcaaaat gacgcatgat
541 tatcttttac gtgactttta agatttaact catacgataa ttatattgtt atttcatgtt
601 ctacttacgt gataacttat tatatatata ttttcttgtt atagatatct aagatacatt
661 gatgagtttg gacaaaccac tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
721 gcccaccctc gtgaggctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc
781 ccgagaagtt ggggggaggg gtcggcaatt gaaccggtgc ctagagaagg tggcgcgggg
841 taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac
901 cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa
961 cacaggtaag tgccgtgtgt ggttcccgcg ggcctggcct ctttacgggt tatggccctt
1021 gcgtgccttg aattacttcc acctggctgc agtacgtgat tcttgatccc gagcttcggg
1081 ttggaagtgg gtgggagagt tcgaggcctt gcgcttaagg agccccttcg cctcgtgctt
1141 gagttgaggc ctggcctggg cgctggggcc gccgcgtgcg aatctggtgg caccttcgcg
1201 cctgtctcgc tgctttcgat aagtctctag ccatttaaaa tttttgatga cctgctgcga
1261 cgcttttttt ctggcaagat agtcttgtaa atgcgggcca agatctgcac actggtattt
1321 cggtttttgg ggccgcgggc ggcgacgggg cccgtgcgtc ccagcgcaca tgttcggcga
1381 ggcggggcct gcgagcgcgg ccaccgagaa tcggacgggg gtagtctcaa gctggccggc
1441 ctgctctggt gcctagtctc gcgccgccgt gtatcgcccc gccctgggcg gcaaggctgg
1501 cccggtcggc accagttgcg tgagcggaaa gatggccgct tcccggccct gctgcaggga
1561 gctcaaaatg gaggacgcgg cgctcgggag agcgggcggg tgagtcaccc acacaaagga
1621 aaagggcctt tccgtcctca gccgtcgctt catgtgactc cacggagtac cgggcgctgt
1681 ccaggcacct cgattagttc tcgagctttt ggagtacgtc gtctttaggt tggggggagg
1741 ggttttatgc gatggagttt ccccacactg agtgggtgga gactgaagtt aggccagctt
1801 ggcacttgat gtaattctcc ttggaatttg ccctttttga gtttggatct tggttcattc
1861 tcaagcctca gacagtggtt caaagttttt ttcttccatt tcaggtgtcg tgagtgaacc
1921 gtcagatccg ctagccacca tggtgagcaa gggcgaggag gataacatgg cctctctccc
1981 agcgacacat gagttacaca tctttggctc catcaacggt gtggactttg acatggtggg
2041 tcagggcacc ggcaatccaa atgatggtta tgaggagtta aacctgaagt ccaccaaggg
2101 tgacctccag ttctccccct ggattctggt ccctcatatc gggtatggct tccatcagta
2161 cctgccctac cctgacggga tgtcgccttt ccaggccgcc atggtagatg gctccggcta
2221 ccaagtccat cgcacaatgc agtttgaaga tggtgcctcc cttactgtta actaccgcta
2281 cacctacgag ggaagccaca tcaaaggaga ggcccaggtg aaggggactg gtttccctgc
2341 tgacggtcct gtgatgacca actcgctgac cgctgcggac tggtgcaggt cgaagaagac
2401 ttaccccaac gacaaaacca tcatcagtac ctttaagtgg agttacacca ctggaaatgg
2461 caagcgctac cggagcactg cgcggaccac ctacaccttt gccaagccaa tggcggctaa
2521 ctatctgaag aaccagccga tgtacgtgtt ccgtaagacg gagctcaagc actccaagac
2581 cgagctcaac ttcaaggagt ggcaaaaggc ctttaccgat gtgatgggca tggacgagct
2641 gtacaagtcc ggactcagat cttcggatcc accggtcgcc accatggcga cccgcagccc
2701 tggcgtcgtg attagtgatg atgaaccagg ttatgacctt gatttatttt gcatacctaa
2761 tcattatgct gaggatttgg aaagggtgtt tattcctcat ggactaatta tggacaggac
2821 tgaacgtctt gctcgagatg tgatgaagga gatgggaggc catcacattg tagccctctg
2881 tgtgctcaag gggggctata aattctttgc tgacctgctg gattacatca aagcactgaa
2941 tagaaatagt gatagatcca ttcctatgac tgtagatttt atcagactga agagctattg
3001 taatgaccag tcaacagggg acataaaagt aattggtgga gatgatctct caactttaac
3061 tggaaagaat gtcttgattg tggaagatat aattgacact ggcaaaacaa tgcagacttt
3121 gctttccttg gtcaggcagt ataatccaaa gatggtcaag gtcgcaagct tgctggtgaa
3181 aaggacccca cgaagtgttg gatataagcc agactttgtt ggatttgaaa ttccagacaa
3241 gtttgttgta ggatatgccc ttgactataa tgaatacttc agggatttga atcatgtttg
3301 tgtcattagt gaaactggaa aagcaaaata caaagccgga tccggagagg gcagaggaag
3361 tctgctaaca tgcggtgacg tcgaggagaa tcctggccca atgctccaga agaagctgga
3421 ggagctggag ctggccaagc ctttgtctca agaagaatcc accctcattg aaagagcaac
3481 ggctacaatc aacagcatcc ccatctctga agactacagc gtcgccagcg cagctctctc
3541 tagcgacggc cgcatcttca ctggtgtcaa tgtatatcat tttactgggg gaccttgtgc
3601 agaactcgtg gtgctgggca ctgctgctgc tgcggcagct ggcaacctga cttgtatcgt
3661 cgcgatcgga aatgagaaca ggggcatctt gagcccctgc ggacggtgcc gacaggtgct
3721 tctcgatctg catcctggga tcaaagccat agtgaaggac agtgatggac agccgacggc
3781 agttgggatt cgtgaattgc tgccctctgg ttatgtgtgg gagggctaag cacttcgtgg
3841 ccgaggagca ggactgacac gtgctacgag atttcgattc caccgccgcc ttctatgaaa
3901 ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc
3961 tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat
4021 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
4081 gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgagt gaagacgaaa
4141 gggctggggg aggtgtggga ggttttttga attagtctct aattgaatta gatcctaaaa
4201 gttttgttac tttatagaag aaattttgag tttttgtttt tttttaataa ataaataaac
4261 ataaataaat tgtttgttga atttattatt agtatgtaag tgtaaatata ataaaactta
4321 atatctattc aaattaataa ataaacctcg atatacagac cgataaaaca catgcgtcaa
4381 ttttacgcat gattatcttt aacgtacgtc acaatatgat tatctttcta gggtaagggc
4441 gaattctgca gatatccatc acactggcgg ccgctcgagc atgcatctag agggcccaat
4501 tcgccctata gtgagtcgta ttacaattca ctggccgtcg ttttacaacg tcgtgactgg
4561 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
4621 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctatacgta
4681 cggcagttta aggtttacac ctataaaaga gagagccgtt atcgtctgtt tgtggatgta
4741 cagagtgata ttattgacac gccggggcga cggatggtga tccccctggc cagtgcacgt
4801 ctgctgtcag ataaagtctc ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc
4861 tggcgcatga tgaccaccga tatggccagt gtgccggtct ccgttatcgg ggaagaagtg
4921 gctgatctca gccaccgcga aaatgacatc aaaaacgcca ttaacctgat gttctgggga
4981 atataaatgt caggcatgag attatcaaaa aggatcttca cctagatcct tttcacgtag
5041 aaagccagtc cgcagaaacg gtgctgaccc cggatgaatg tcagctactg ggctatctgg
5101 acaagggaaa acgcaagcgc aaagagaaag caggtagctt gcagtgggct tacatggcga
5161 tagctagact gggcggtttt atggacagca agcgaaccgg aattgccagc tggggcgccc
5221 tctggtaagg ttgggaagcc ctgcaaagta aactggatgg ctttcttgcc gccaaggatc
5281 tgatggcgca ggggatcaag ctctgatcaa gagacaggat gaggatcgtt tcgcatgatt
5341 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat
5401 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag
5461 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaagac
5521 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac
5581 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc
5641 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg
5701 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag
5761 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat
5821 caggggctcg cgccagccga actgttcgcc aggctcaagg cgagcatgcc cgacggcgag
5881 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc
5941 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg
6001 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg
6061 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag
6121 ttcttctgaa ttattaacgc ttacaatttc ctgatgcggt attttctcct tacgcatctg
6181 tgcggtattt cacaccgcat caggtggcac ttttcgggga aatgtgcgcg gaacccctat
6241 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
6301 aatgcttcaa taatagcacg tgaggagggc caccatggcc aagttgacca gtgccgttcc
6361 ggtgctcacc gcgcgcgacg tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt
6421 ctcccgggac ttcgtggagg acgacttcgc cggtgtggtc cgggacgacg tgaccctgtt
6481 catcagcgcg gtccaggacc aggtggtgcc ggacaacacc ctggcctggg tgtgggtgcg
6541 cggcctggac gagctgtacg ccgagtggtc ggaggtcgtg tccacgaact tccgggacgc
6601 ctccgggccg gccatgaccg agatcggcga gcagccgtgg gggcgggagt tcgccctgcg
6661 cgacccggcc ggcaactgcg tgcacttcgt ggccgaggag caggactgac acgtgctaaa
6721 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
6781 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
6841 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
6901 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
6961 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
7021 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
7081 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
7141 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
7201 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
7261 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
7321 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
7381 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
7441 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
7501 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
7561 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaag
//
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pWZ853质粒载体应用举例
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