| 质粒名称: | pCF159_BRL |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 225962 |
| 存储实验室: | Christof Fellmann |
|
载体骨架基本信息 |
|
| 载体名称: | pCF159 |
| 载体出品公司: | Clontech |
| 完整质粒大小: | 6527bp |
| 载体类型: | 哺乳细胞表达 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | CMV-Intron-BRL (env protein) |
| 物种来源: | Baboon endogenous retrovirus env protein BRL (BaEVRLess) |
|
文献引用方法 |
|
| 材料和方法部分: | pCF159_BRL was a gift from Christof Fellmann (Addgene plasmid # 225962 ; http://n2t.net/addgene:225962 ; RRID:Addgene_225962) |
| 参考文献部分: | Neuronal DNA repair reveals strategies to influence CRISPR editing outcomes. Ramadoss GN, Namaganda SJ, Hamilton JR, Sharma R, Chow KG, Macklin BL, Sun M, Liu JC, Fellmann C, Watry HL, Jin J, Perez BS, Sandoval Espinoza CR, Matia MP, Lu SH, Judge LM, Nussenzweig A, Adamson B, Murthy N, Doudna JA, Kampmann M, Conklin BR. bioRxiv [Preprint]. 2024 Jun 26:2024.06.25.600517. doi: 10.1101/2024.06.25.600517. 10.1101/2024.06.25.600517 PubMed 38979269 |
买家导航
pCF159_BRL载体质粒基本信息
pCF159_BRL质粒图谱载体图谱和pCF159_BRL载体序列质粒序列多克隆位点信息
pCF159_BRL质粒载体简介
pCF159_BRL质粒序列载体序列
LOCUS pCF159_BRL 6527 bp DNA circular SYN 14-APR-2025
DEFINITION CMV-Intron-BRL (env protein). Expresses BRL (BaEVRLess) env for VLP
production..
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6527)
AUTHORS Ramadoss GN, Namaganda SJ, Hamilton JR, Sharma R, Chow KG, Macklin
BL, Sun M, Liu JC, Fellmann C, Watry HL, Jin J, Perez BS, Sandoval
Espinoza CR, Matia MP, Lu SH, Judge LM, Nussenzweig A, Adamson B,
Murthy N, Doudna JA, Kampmann M, Conklin BR
TITLE Neuronal DNA repair reveals strategies to influence CRISPR editing
outcomes.
JOURNAL bioRxiv [Preprint]. 2024 Jun 26:2024.06.25.600517. doi:
10.1101/2024.06.25.600517.
PUBMED 38979269
REFERENCE 2 (bases 1 to 6527)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 14, 2025 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv
[Preprint]. 2024 Jun 26:2024.06.25.600517. doi:
10.1101/2024.06.25.600517."
COMMENT Sequence Label: pCF159_BRL
FEATURES Location/Qualifiers
source 1..6527
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 97..476
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 477..680
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 631..651
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 677..701
/label=LNCX
/note="Human CMV promoter, forward primer"
intron 792..1364
/label=beta-globin intron
/note="intron from rabbit beta-globin gene"
primer_bind complement(3232..3251)
/label=Bglob-pA-R
/note="Rabbit beta-globin polyA region, reverse primer"
polyA_signal 3297..3352
/label=beta-globin poly(A) signal
/note="rabbit beta-globin polyadenylation signal (Gil and
Proudfoot, 1987)"
primer_bind complement(3351..3370)
/label=rbglobpA-R
/note="Rabbit beta-globin polyA, reverse primer. Also
called rb-glob-pA-term-R"
primer_bind complement(3710..3726)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(3710..3726)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(3723..3745)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 3734..3750
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(join(3758..3764,3765..3782,3783..3788))
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 3803..3824
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(3941..3958)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(4112..4700)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(4192..4211)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(join(4871..5662,5663..5731))
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 5494..5513
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(5732..5836)
/gene="bla"
/label=AmpR promoter
rep_origin complement(5862..6317)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(5999..6020)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 6211..6230
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 6444..6466
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 6458..6475
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 6459..6475
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 6485..6504
/label=T7
/note="T7 promoter, forward primer"
promoter 6485..6503
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
1 gcggccgctc tagagagctt ggcccattgc atacgttgta tccatatcat aatatgtaca
61 tttatattgg ctcatgtcca acattaccgc catgttgaca ttgattattg actagttatt
121 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
181 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
241 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
301 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
361 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
421 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
481 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
541 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
601 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
661 aggtctatat aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac
721 gctgttttga cctccataga agacaccggg accgatccag cctccggtcg accgatcctg
781 agaacttcag ggtgagtttg gggacccttg attgttcttt ctttttcgct attgtaaaat
841 tcatgttata tggagggggc aaagttttca gggtgttgtt tagaatggga agatgtccct
901 tgtatcacca tggaccctca tgataatttt gtttctttca ctttctactc tgttgacaac
961 cattgtctcc tcttattttc ttttcatttt ctgtaacttt ttcgttaaac tttagcttgc
1021 atttgtaacg aatttttaaa ttcacttttg tttatttgtc agattgtaag tactttctct
1081 aatcactttt ttttcaaggc aatcagggta tattatattg tacttcagca cagttttaga
1141 gaacaattgt tataattaaa tgataaggta gaatatttct gcatataaat tctggctggc
1201 gtggaaatat tcttattggt agaaacaact acaccctggt catcatcctg cctttctctt
1261 tatggttaca atgatataca ctgtttgaga tgaggataaa atactctgag tccaaaccgg
1321 gcccctctgc taaccatgtt catgccttct tctctttcct acagctcctg ggcaacgtgc
1381 tggttgttgt gctgtctcat cattttggca aagctcgagc tgtatttgtg ccaacatggg
1441 attcacaaca aagataatct tcttatacaa cctagtactg gtctacgcgg ggtttgacga
1501 ccctcgcaaa gccatagaac tagtacaaaa gcgatatggc cgaccatgcg attgcagcgg
1561 aggacaagtg tccgagcccc cgtcagacag ggtcagtcaa gtgacttgct caggcaagac
1621 agcttactta atgcccgacc aaagatggaa atgtaagtca attccaaaag acacctcccc
1681 aagcgggcca ctccaagagt gcccctgtaa ttcttaccag tcctcagtac acagttcttg
1741 ttatacctca taccaacaat gcagatcagg caataagaca tattatacgg ctactctgct
1801 aaaaacacaa actgggggca ccagtgatgt acaagtatta ggatccacca acaaacttat
1861 acaatctccc tgtaatggca taaaagggca gtctatttgc tggagcacta cagctcctat
1921 ccacgtctct gatggaggag gtccattaga caccacaaga attaaaagtg ttcagagaaa
1981 actggaagaa attcataaag ccctatatcc tgaacttcag tatcaccctt tggccatacc
2041 taaggttaga gataacctca tggtcgatgc ccagacttta aacattctca atgccactta
2101 caacttactc ctaatgtcca acacgagcct agtggacgac tgttggcttt gtttaaaatt
2161 aggtccccct actcccctcg caatacctaa cttcctatta tcctacgtga ctcgctcctc
2221 ggataatatc tcttgtttaa taattccccc ccttctagtt caaccgatgc agttttccaa
2281 ttcatcttgc ctcttttccc cctcctacaa cagtacagaa gaaatagatc taggccatgt
2341 tgccttcagc aactgtacct ccataaccaa tgtcaccggt cccatatgcg ctgtaaatgg
2401 ttcggtcttt ctctgtggca ataacatggc atacacttat ctacccacga actggacggg
2461 gctttgcgtc ctagcaactc tcctccccga cattgacatc attcccggag atgaaccggt
2521 ccccatccct gctattgatc attttatata tagacctaaa cgggccatac agtttattcc
2581 tttactagca gggctaggga tcaccgcagc cttcacaaca ggagctacag gcctaggtgt
2641 ctctgtgacc caatatacaa aattatctaa tcagctaatt tctgatgtac aaatcttatc
2701 tagcaccata caagatctgc aagatcaagt agactcatta gccgaagtgg ttctccagaa
2761 cagaaggggg ctagatctac ttacagcaga acaaggagga atctgtttag ccctgcaaga
2821 aaaatgctgc ttttatgtta acaagtcagg gattgtgaga gacaaaataa aaaccttaca
2881 agaagaacta gaaagacgta gaaaagatct agcttccaac ccactttgga ctgggcttca
2941 agggctcctc ccttacctcc tgccctttct tggccctcta cttaccctcc tgctcttact
3001 caccattggg ccgtgcattt ttaaccgtct aaccgctttt attaatgata agttaaacat
3061 aatacacgct atgtagttaa cccaacagta tcaggtgctc agaaccgatg aagaagctca
3121 agattgagcc tctaagacac aagaaaagga attcactcct caggtgcagg ctgcctatca
3181 gaaggtggtg gctggtgtgg ccaatgccct ggctcacaaa taccactgag atctttttcc
3241 ctctgccaaa aattatgggg acatcatgaa gccccttgag catctgactt ctggctaata
3301 aaggaaattt attttcattg caatagtgtg ttggaatttt ttgtgtctct cactcggaag
3361 gacatatggg agggcaaatc atttaaaaca tcagaatgag tatttggttt agagtttggc
3421 aacatatgcc catatgctgg ctgccatgaa caaaggttgg ctataaagag gtcatcagta
3481 tatgaaacag ccccctgctg tccattcctt attccataga aaagccttga cttgaggtta
3541 gatttttttt atattttgtt ttgtgttatt tttttcttta acatccctaa aattttcctt
3601 acatgtttta ctagccagat ttttcctcct ctcctgacta ctcccagtca tagctgtccc
3661 tcttctctta tggagatccc tcgacggatc ggccgcaatt cgtaatcatg tcatagctgt
3721 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
3781 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
3841 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
3901 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
3961 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
4021 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
4081 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
4141 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
4201 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
4261 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
4321 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
4381 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
4441 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
4501 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
4561 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
4621 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
4681 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
4741 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
4801 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
4861 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
4921 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
4981 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
5041 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
5101 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
5161 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
5221 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
5281 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
5341 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
5401 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
5461 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
5521 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
5581 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
5641 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
5701 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
5761 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
5821 aaataggggt tccgcgcaca tttccccgaa aagtgccacc taaattgtaa gcgttaatat
5881 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga
5941 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc
6001 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac
6061 cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc
6121 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg
6181 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag
6241 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc
6301 gccgctacag ggcgcgtccc attcgccatt caggctgcgc aactgttggg aagggcgatc
6361 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt
6421 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgagcg
6481 cgcgtaatac gactcactat agggcgaatt ggagctccac cgcggtg
//
