| 出品人: | Bob Weinberg |
|---|---|
| 载体名称: | pBABE-neo， pBABEneo, addgene#1767 |
| 质粒类型: | 逆病毒载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | MSCV |
| 载体大小: | 5277 bp |
| 5' 测序引物及序列: | pBABE 5' primer 5'CTTTATCCAGCCCTCAC3' |
| 3' 测序引物及序列: | pBABE 3' primer 5'ACCCTAACTGACACACATTCC3' |
| 载体标签: | 无 |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | Neomycin |
| 克隆菌株: | DH5α、HB101等 |
| 宿主细胞（系）: | 常规哺乳动物细胞系 |
| 备注: |
推荐使用逆病毒包装载体：pUMVC、pCMV-VSV-G 。 |
| 产品目录号: | 1767 |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 逆转录病毒 |
买家导航
pBABE-neo载体 pBABEneo质粒基本信息
pBABE-neo质粒图谱 pBABEneo载体图谱和pBABE-neo载体序列 pBABEneo质粒序列多克隆位点信息
pBABE-neo质粒 pBABEneo载体简介
pBABE-neo质粒序列 pBABEneo载体序列
LOCUS pbabe-neo 5277 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Retroviral vector for cloning and expressing your gene of interest.
Neomycin selection..
ACCESSION .
VERSION .
KEYWORDS pBABE-neo
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5277)
AUTHORS Morgenstern JP, Land H
TITLE Advanced mammalian gene transfer: high titre retroviral vectors with
multiple drug selection markers and a complementary helper-free
packaging cell line.
JOURNAL Nucleic Acids Res. 1990 Jun 25;18(12):3587-96.
PUBMED 2194165
REFERENCE 2 (bases 1 to 5277)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..5277
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 8..477
/note="long terminal repeat from Moloney murine leukemia
virus"
misc_feature 542..741
/label=MMLV Psi
/note="packaging signal of Moloney murine leukemia virus
(MMLV)"
CDS 942..1358
/label=gag (truncated)
/note="truncated Moloney murine leukemia virus (MMLV) gag
gene lacking the start codon"
primer_bind 1288..1310
/label=pLXSN 5'
/note="Murine stem cell virus, forward primer. Also called
MSCV"
primer_bind 1326..1342
/label=pBABE 5'
/note="Psi packaging signal, forward primer for pBABE
vectors"
primer_bind complement(1416..1436)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 1421..1750
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 1601..1736
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 1663..1682
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 2107..2901
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(2161..2180)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 2771..2790
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind 3424..3443
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
LTR 3705..4174
/note="long terminal repeat from Moloney murine leukemia
virus"
CDS complement(4289..5149)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 4912..4931
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(5150..5254)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 ctgcagcctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca
61 gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt
121 tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag
181 tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc
241 ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga
301 gctcaataaa agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc
361 gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc
421 tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcattt
481 gggggctcgt ccgggatcgg gagacccctg cccagggacc accgacccac caccgggagg
541 caagctggcc agcaacttat ctgtgtctgt ccgattgtct agtgtctatg actgatttta
601 tgcgcctgcg tcggtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa
661 ctgacgagtt ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc
721 gtttttgtgg cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg
781 tggttctggt aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt
841 cggtttggaa ccgaagccgc gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt
901 gtctctgtct gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc
961 ccttaagttt gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg
1021 tagatgtcaa gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg
1081 tcggatggcc gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg
1141 tcttttcacc tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag
1201 ccttggcttt tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc
1261 ctcttcctcc atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat
1321 cctcccttta tccagccctc actccttctc taggcgccgg ccggatccca gtgtggtggt
1381 acgtaggaat tcgccagcac agtggtcgac cctgtggaat gtgtgtcagt tagggtgtgg
1441 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
1501 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
1561 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
1621 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
1681 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
1741 cttttgcaaa aagcttcacg ctgccgcaag cactcagggc gcaagggctg ctaaaggaag
1801 cggaacacgt agaaagccag tccgcagaaa cggtgctgac cccggatgaa tgtcagctac
1861 tgggctatct ggacaaggga aaacgcaagc gcaaagagaa agcaggtagc ttgcagtggg
1921 cttacatggc gatagctaga ctgggcggtt ttatggacag caagcgaacc ggaattgcca
1981 gctggggcgc cctctggtaa ggttgggaag ccctgcaaag taaactggat ggctttcttg
2041 ccgccaagga tctgatggcg caggggatca agatctgatc aagagacagg atgaggatcg
2101 tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg
2161 ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg
2221 ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat
2281 gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca
2341 gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg
2401 gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat
2461 gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa
2521 catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg
2581 gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg
2641 cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg
2701 gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat
2761 caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac
2821 cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc
2881 cttcttgacg agttcttctg agcgggactc tggggttcgc atcgatgcat cgatgcggcg
2941 ggtgtggtgg ttacgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
3001 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
3061 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
3121 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
3181 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
3241 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
3301 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
3361 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
3421 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
3481 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcatc
3541 gataaaataa aagattttat ttagtctcca gaaaaagggg ggaatgaaag accccacctg
3601 taggtttggc aagctagctt aagtaacgcc attttgcaag gcatggaaaa atacataact
3661 gagaatagag aagttcagat caaggtcagg aacagatgga acagctgaat atgggccaaa
3721 caggatatct gtggtaagca gttcctgccc cggctcaggg ccaagaacag atggaacagc
3781 tgaatatggg ccaaacagga tatctgtggt aagcagttcc tgccccggct cagggccaag
3841 aacagatggt ccccagatgc ggtccagccc tcagcagttt ctagagaacc atcagatgtt
3901 tccagggtgc cccaaggacc tgaaatgacc ctgtgcctta tttgaactaa ccaatcagtt
3961 cgcttctcgc ttctgttcgc gcgcttctgc tccccgagct caataaaaga gcccacaacc
4021 cctcactcgg ggcgccagtc ctccgattga ctgagtcgcc cgggtacccg tgtatccaat
4081 aaaccctctt gcagttgcat ccgacttgtg gtctcgctgt tccttgggag ggtctcctct
4141 gagtgattga ctacccgtca gcgggggtct ttcacatgca gcatgtatca aaattaattt
4201 ggtttttttt cttaagtatt tacattaaat ggccatagtg cggccgcaaa tcaatctaaa
4261 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
4321 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
4381 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
4441 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
4501 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
4561 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
4621 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
4681 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
4741 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
4801 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
4861 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
4921 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
4981 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
5041 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
5101 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
5161 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
5221 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccac
//
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