| 质粒名称: | pTargetF-FRT |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 113637 |
| 存储实验室: | Olivier Lichtarge |
|
载体骨架基本信息 |
|
| 载体名称: | pTargetF |
| 完整质粒大小: | 2116 bp |
| 载体类型: | CRISPR表达 |
| 载体抗性: | 大观霉素和链霉素，50 & 50 μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 低拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | sgRNA-FRT |
| 物种来源: | Synthetic |
| 启动子: | pJ23119 |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | SpeI（未销毁） |
| 3’克隆位点: | SpeI（未销毁） |
| 5’测序引物 | pTarget-1 |
| 3’测序引物 | pTarget-2 |
|
文献引用方法 |
|
| 材料和方法部分: | pTargetF-FRT was a gift from Olivier Lichtarge (Addgene plasmid # 113637 ; http://n2t.net/addgene:113637 ; RRID:Addgene_113637) |
| 参考文献部分: | CRISPR-FRT targets shared sites in a knock-out collection for off-the-shelf genome editing. Swings T, Marciano DC, Atri B, Bosserman RE, Wang C, Leysen M, Bonte C, Schalck T, Furey I, Van den Bergh B, Verstraeten N, Christie PJ, Herman C, Lichtarge O, Michiels J. Nat Commun. 2018 Jun 8;9(1):2231. doi: 10.1038/s41467-018-04651-5. 10.1038/s41467-018-04651-5 [pii] PubMed 29884781 |
买家导航
pTargetF-FRT载体质粒基本信息
pTargetF-FRT质粒图谱载体图谱和pTargetF-FRT载体序列质粒序列多克隆位点信息
pTargetF-FRT质粒载体简介
pTargetF-FRT质粒序列载体序列
LOCUS pTargetF-FRT 2115 bp DNA circular SYN 15-DEC-2025
DEFINITION Derivative of pTargetF with gRNA targeting FRT site.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 2115)
AUTHORS Swings T, Marciano DC, Atri B, Bosserman RE, Wang C, Leysen M, Bonte
C, Schalck T, Furey I, Van den Bergh B, Verstraeten N, Christie PJ,
Herman C, Lichtarge O, Michiels J
TITLE CRISPR-FRT targets shared sites in a knock-out collection for
off-the-shelf genome editing.
JOURNAL Nat Commun. 2018 Jun 8;9(1):2231. doi: 10.1038/s41467-018-04651-5.
PUBMED 29884781
REFERENCE 2 (bases 1 to 2115)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Dec 15, 2025 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Nat
Commun."; date: "2018-06-8"; volume: "9(1):2231. doi"; pages: "
10.1038/s41467-018-04651-5"
COMMENT Sequence Label: pTargetF-FRT
FEATURES Location/Qualifiers
source 1..2115
/mol_type="other DNA"
/organism="synthetic DNA construct"
CDS 124..915
/codon_start=1
/gene="aadA"
/product="aminoglycoside adenylyltransferase (Murphy,
1985)"
/label=SmR
/note="confers resistance to spectinomycin and
streptomycin"
/translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
rep_origin 1089..1677
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 1578..1597
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 1831..1848
/label=L4440
/note="L4440 vector, forward primer"
promoter 1925..1959
/label=J23119(SpeI) promoter
/note="bacterial promoter (Registry of Standard Biological
Parts BBa_J23119) modified to end with an SpeI site"
misc_RNA 1978..2053
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
ORIGIN
1 tcgagttcat gtgcagctcc ataagcaaaa ggggatgata agtttatcac caccgactat
61 ttgcaacagt gccgttgatc gtgctatgat cgactgatgt catcagcggt ggagtgcaat
121 gtcatgaggg aagcggtgat cgccgaagta tcgactcaac tatcagaggt agttggcgtc
181 atcgagcgcc atctcgaacc gacgttgctg gccgtacatt tgtacggctc cgcagtggat
241 ggcggcctga agccacacag tgatattgat ttgctggtta cggtgaccgt aaggcttgat
301 gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa cttcggcttc ccctggagag
361 agcgagattc tccgcgctgt agaagtcacc attgttgtgc acgacgacat cattccgtgg
421 cgttatccag ctaagcgcga actgcaattt ggagaatggc agcgcaatga cattcttgca
481 ggtatcttcg agccagccac gatcgacatt gatctggcta tcttgctgac aaaagcaaga
541 gaacatagcg ttgccttggt aggtccagcg gcggaggaac tctttgatcc ggttcctgaa
601 caggatctat ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg
661 gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca tttggtacag cgcagtaacc
721 ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct gccggcccag
781 tatcagcccg tcatacttga agctagacag gcttatcttg gacaagaaga agatcgcttg
841 gcctcgcgcg cagatcagtt ggaagaattt gtccactacg tgaaaggcga gatcaccaag
901 gtagtcggca aataagatgc cgctcgccag tcgattggct gagctcataa gttcctattc
961 cgaagttccg cgaacgcgta aaggatctag gtgaagatcc tttttgataa tctcatgacc
1021 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
1081 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
1141 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
1201 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
1261 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
1321 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
1381 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
1441 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
1501 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
1561 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
1621 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
1681 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
1741 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
1801 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
1861 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatgctgg
1921 atccttgaca gctagctcag tcctaggtat aatactagtc tatactttct agagaatgtt
1981 ttagagctag aaatagcaag ttaaaataag gctagtccgt tatcaacttg aaaaagtggc
2041 accgagtcgg tgcttttttt gaattctcta gagtcgacct gcagaagctt agatctatta
2101 ccctgttatc cctac
//
pTargetF-FRT载体图谱质粒图谱pdf版和pTargetF-FRT载体序列质粒序列等相关资料下载
pTargetF-FRT质粒载体应用举例
- 上一篇：pCMV-Mm.cargo(Cre)
- 下一篇：没有了
