| 质粒名称: | pGL3-NMB-Nicking/PegRNA-PGK-puroR |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002175 |
| 产品用途: | The hCtRNA simultaneously drives the expression of both the targeting NMB gene NickingRNA and pegRNA, harboring selection markers of puroR. |
| 存储实验室: | Zhuobin Liang Lab 梁卓斌课题组 |
|
载体骨架基本信息 |
|
| 载体名称: | pGL3 |
| 表达系统: | 哺乳动物表达 |
| 启动子: | hCtRNA |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | NMB-Nicking-PegRNA |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
TRU-PE: A Universal, Trackable Prime Editor Toolkit for Robust Single-and Multi-Locus Genome Engineering
Zhichao Qiu, Keke Sun, Qingwei Zeng, Ziwei Luo, Xinran Liu, Yaping Li, Xiang Lei, Ruilin Zhao, Zhen Zhang, Damia Romero-Moya, Yuan Yang, Xuan Wang, Ehsan Hashemi, Joe Z. Zhang, Xiaolong Wang, Alessandra Giorgetti, Zhuobin Liang
bioRxiv 2026.02.20.706938
|
pGL3-NMB-Nicking/PegRNA-PGK-puroR
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pGL3-NMB-Nicking/PegRNA-PGK-puroR载体质粒基本信息
pGL3-NMB-Nicking/PegRNA-PGK-puroR质粒图谱载体图谱和pGL3-NMB-Nicking/PegRNA-PGK-puroR载体序列质粒序列多克隆位点信息
pGL3-NMB-Nicking/PegRNA-PGK-puroR质粒载体简介
pGL3-NMB-Nicking/PegRNA-PGK-puroR质粒序列载体序列
LOCUS pGL3-NMB-Nicking/PegRNA-PGK-puroR 4942 bp DNA circular SYN 07-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4942)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 19, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..4942
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 14..31
/label=OL1400
primer_bind complement(31..54)
/label=OL459
primer_bind 46..70
/label=OL535
misc_feature 51..125
/label=hCtRNA
primer_bind 66..89
/label=OL464
primer_bind complement(106..145)
/label=OL541
primer_bind complement(106..126)
/label=OL543
primer_bind complement(108..126)
/label=OL465
primer_bind 126..169
/label=OL542
misc_feature 126..145
/label=NMB-nickingRNA
primer_bind 145..186
/label=OL462
misc_RNA 146..221
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
primer_bind complement(204..240)
/label=OL463
misc_feature 222..296
/label=hCtRNA
primer_bind 222..241
/label=OL535
primer_bind 237..260
/label=OL464
primer_bind complement(277..316)
/label=OL543
primer_bind complement(277..297)
/label=OL541
primer_bind complement(279..297)
/label=OL465
primer_bind 297..326
/label=OL544
misc_feature 297..316
/label=NMB spacer
misc_RNA 317..392
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
primer_bind 317..357
/label=OL462
primer_bind 317..340
/label=OL542
primer_bind complement(375..395)
/label=OL463
misc_feature 393..406
/label=RTT
primer_bind complement(397..426)
/label=OL545
misc_feature 407..417
/label=PBS
primer_bind complement(409..435)
/label=OL1402
primer_bind 423..442
/label=OL458
misc_feature 461..578
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 627..1137
/label=hPGK promoter
/note="human phosphoglycerate kinase 1 promoter"
CDS 1159..1758
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
polyA_signal 1905..2026
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(2445..3033)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(3204..4064)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(4065..4169)
/gene="bla"
/label=AmpR promoter
rep_origin 4196..4651
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
polyA_signal 4782..4830
/note="synthetic polyadenylation signal"
misc_feature 4844..4935
/label=pause site
/note="RNA polymerase II transcriptional pause signal from
the human alpha-2 globin gene"
ORIGIN
1 ggtaccgatt agtgaacgga tctcgacggt atcgatcacg agactagcct agagggggta
61 tagctcagtg gtagagcatt tgactgcaga tcaagaggtc cccggttcaa atccgggtgc
121 ccccttcctt tccaggtcac ttcatgtttt agagctagaa atagcaagtt aaaataaggc
181 tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg cagagggggt atagctcagt
241 ggtagagcat ttgactgcag atcaagaggt ccccggttca aatccgggtg cccccttggt
301 ccctcaggga ggtgtggttt tagagctaga aatagcaagt taaaataagg ctagtccgtt
361 atcaacttga aaaagtggca ccgagtcggt gcggacagct acccacacct ccctgagttt
421 ttttctcgac ctcgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
481 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
541 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
601 gagatccact ttggccgcgg ctcgaggggg ttggggttgc gccttttcca aggcagccct
661 gggtttgcgc agggacgcgg ctgctctggg cgtggttccg ggaaacgcag cggcgccgac
721 cctgggactc gcacattctt cacgtccgtt cgcagcgtca cccggatctt cgccgctacc
781 cttgtgggcc ccccggcgac gcttcctgct ccgcccctaa gtcgggaagg ttccttgcgg
841 ttcgcggcgt gccggacgtg acaaacggaa gccgcacgtc tcactagtac cctcgcagac
901 ggacagcgcc agggagcaat ggcagcgcgc cgaccgcgat gggctgtggc caatagcggc
961 tgctcagcag ggcgcgccga gagcagcggc cgggaagggg cggtgcggga ggcggggtgt
1021 ggggcggtag tgtgggccct gttcctgccc gcgcggtgtt ccgcattctg caagcctccg
1081 gagcgcacgt cggcagtcgg ctccctcgtt gaccgaatca ccgacctctc tccccagggg
1141 gatccaccgg agcttaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac
1201 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc
1261 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc
1321 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg
1381 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc
1441 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg
1501 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac
1561 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc
1621 ggggtgcccg ccttcctgga aacctccgcg ccccgcaacc tccccttcta cgagcggctc
1681 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc
1741 cgcaagcccg gtgcctgacg cccgccccac gacccgcagc gcccgaccga aaggagcgca
1801 cgaccccatg catcggtacc tttaagacca atgacttaca aggcagctgt agatcttagc
1861 cactttctag agtcggggcg gccggccgct tcgagcagac atgataagat acattgatga
1921 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
1981 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg
2041 cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa
2101 cctctacaaa tgtggtaaaa tcgataagga tccgtcgacc gatgcccttg agagccttca
2161 acccagtcag ctccttccgg tgggcgcggg gcatgactat cgtcgccgca cttatgactg
2221 tcttctttat catgcaactc gtaggacagg tgccggcagc gctcttccgc ttcctcgctc
2281 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
2341 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
2401 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
2461 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
2521 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
2581 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
2641 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
2701 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
2761 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
2821 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
2881 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
2941 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
3001 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
3061 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
3121 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
3181 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
3241 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
3301 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgggaccc acgctcaccg
3361 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
3421 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
3481 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
3541 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
3601 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
3661 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
3721 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
3781 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
3841 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
3901 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
3961 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
4021 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
4081 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
4141 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgcg
4201 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
4261 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
4321 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
4381 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
4441 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
4501 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
4561 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
4621 aattttaaca aaatattaac gcttacaatt tgccattcgc cattcaggct gcgcaactgt
4681 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agcccaagct accatgataa
4741 gtaagtaata ttaaggtacg ggaggtactt ggagcggccg caataaaata tctttatttt
4801 cattacatct gtgtgttggt tttttgtgtg aatcgatagt actaacatac gctctccatc
4861 aaaacaaaac gaaacaaaac aaactagcaa aataggctgt ccccagtgca agtgcaggtg
4921 ccagaacatt tctctatcga ta
//
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