| 质粒名称: | pGL3-ASXL1-Nicking/EpegRNA-PGK-puroR-mRFP1 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002199 |
| 产品用途: | The hCtRNA simultaneously drives the expression of both the targeting ASXL1 gene NickingRNA and EpegRNA, harboring dual selection markers of puroR and RFP. |
| 存储实验室: | Zhuobin Liang Lab 梁卓斌课题组 |
|
载体骨架基本信息 |
|
| 载体名称: | pGL3 |
| 表达系统: | 哺乳动物表达 |
| 启动子: | hCtRNA |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | ASXL1-nicking-sgRNA-EpegRNA |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
TRU-PE: A Universal, Trackable Prime Editor Toolkit for Robust Single-and Multi-Locus Genome Engineering
Zhichao Qiu, Keke Sun, Qingwei Zeng, Ziwei Luo, Xinran Liu, Yaping Li, Xiang Lei, Ruilin Zhao, Zhen Zhang, Damia Romero-Moya, Yuan Yang, Xuan Wang, Ehsan Hashemi, Joe Z. Zhang, Xiaolong Wang, Alessandra Giorgetti, Zhuobin Liang
bioRxiv 2026.02.20.706938
|
pGL3-ASXL1-Nicking/EpegRNA-PGK-puroR-mRFP1
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
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pGL3-ASXL1-Nicking/EpegRNA-PGK-puroR-mRFP1载体质粒基本信息
pGL3-ASXL1-Nicking/EpegRNA-PGK-puroR-mRFP1质粒图谱载体图谱和pGL3-ASXL1-Nicking/EpegRNA-PGK-puroR-mRFP1载体序列质粒序列多克隆位点信息
pGL3-ASXL1-Nicking/EpegRNA-PGK-puroR-mRFP1质粒载体简介
pGL3-ASXL1-Nicking/EpegRNA-PGK-puroR-mRFP1质粒序列载体序列
LOCUS pGL3-ASXL1-Nicking/EpegRNA-PGK-puroR-mRFP1 5716 bp DNA circular SYN 07-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5716)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 20, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5716
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 1..20
/label=OL2714
primer_bind 4..23
/label=OL1915
primer_bind 4..21
/label=OL1400
primer_bind 12..36
/label=OL2631
primer_bind complement(12..36)
/label=OL2637
primer_bind 17..48
/label=OL2785
primer_bind complement(17..44)
/label=OL2746
primer_bind 18..40
/label=OL2707
primer_bind 21..55
/label=OL2640
primer_bind 24..61
/label=OL2745
primer_bind 37..61
/label=OL1451
misc_feature 41..115
/label=hCtRNA
primer_bind 41..115
/label=OL477
primer_bind complement(98..132)
/label=OL2606
primer_bind complement(98..115)
/label=OL2595
primer_bind complement(98..115)
/label=OL2596
primer_bind complement(98..115)
/label=OL2600
primer_bind complement(98..115)
/label=OL2602
primer_bind complement(98..115)
/label=OL2608
misc_feature 116..135
/label=ASXL1 nickingRNA
primer_bind 126..171
/label=OL2607
primer_bind 133..171
/label=OL2601
primer_bind 135..171
/label=OL2609
primer_bind complement(136..215)
/label=OL476
misc_RNA 136..211
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
primer_bind 136..207
/label=OL2116
primer_bind 136..171
/label=OL2597
primer_bind 136..171
/label=OL2603
primer_bind complement(140..211)
/label=OL2117
primer_bind 140..177
/label=OL1453
primer_bind complement(183..202)
/label=OL1927
primer_bind complement(188..207)
/label=OL1940
primer_bind complement(194..212)
/label=OL2619
primer_bind complement(194..211)
/label=OL2615
primer_bind complement(194..211)
/label=OL2611
primer_bind complement(194..211)
/label=OL769
primer_bind complement(201..211)
/label=OL2116
primer_bind 208..286
/label=OL477
misc_feature 212..286
/label=hCtRNA
primer_bind 212..232
/label=OL1451
primer_bind 212..232
/label=OL2745
primer_bind 212..226
/label=OL2640
primer_bind complement(269..302)
/label=OL2608
primer_bind complement(269..288)
/label=OL2595
primer_bind complement(269..287)
/label=OL2596
primer_bind complement(269..286)
/label=OL2600
primer_bind complement(269..286)
/label=OL2602
primer_bind complement(269..286)
/label=OL2606
misc_feature 287..306
/label=ASXL1 spacer
primer_bind 296..308
/label=OL2764
primer_bind 297..342
/label=OL2609
primer_bind 306..342
/label=OL2601
primer_bind 306..342
/label=OL2607
primer_bind complement(307..383)
/label=OL476
misc_RNA 307..382
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
primer_bind 307..378
/label=OL2116
primer_bind 307..342
/label=OL2597
primer_bind 307..342
/label=OL2603
primer_bind complement(311..382)
/label=OL2117
primer_bind 311..348
/label=OL1453
primer_bind complement(354..373)
/label=OL1927
primer_bind complement(359..378)
/label=OL1940
primer_bind complement(365..406)
/label=OL2619
primer_bind complement(365..382)
/label=OL2615
primer_bind complement(365..382)
/label=OL2611
primer_bind complement(365..382)
/label=OL769
primer_bind complement(372..382)
/label=OL2116
misc_feature 383..396
/label=RTT
primer_bind complement(388..441)
/label=OL2635
primer_bind complement(392..414)
/label=OL2764
primer_bind 395..414
/label=OL2765
primer_bind 396..436
/label=OL2748
primer_bind complement(396..414)
/label=OL2749
misc_feature 397..404
/label=PBS
primer_bind 402..454
/label=OL2620
misc_feature 405..412
/label=Linker
primer_bind complement(406..458)
/label=OL2621
misc_feature 413..449
/label=motif
primer_bind complement(413..447)
/label=OL2614
primer_bind 413..443
/label=OL2613
primer_bind complement(413..441)
/label=OL2634
primer_bind complement(413..440)
/label=OL2618
primer_bind complement(413..439)
/label=OL2632
primer_bind 413..436
/label=OL2617
primer_bind 418..472
/label=OL2633
primer_bind 421..479
/label=OL2636
primer_bind complement(462..481)
/label=OL1916
primer_bind complement(476..492)
/label=OL1168
misc_feature 493..610
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
primer_bind complement(547..566)
/label=OL1936
primer_bind complement(553..577)
/label=OL1571
primer_bind complement(629..648)
/label=OL1088
promoter 659..1169
/label=hPGK promoter
/note="human phosphoglycerate kinase 1 promoter"
primer_bind complement(753..772)
/label=OL1068
primer_bind 901..923
/label=OL1709
primer_bind 943..959
/label=OL446
primer_bind 1088..1106
/label=hPGK-F
/note="Human PGK promoter, forward primer"
primer_bind complement(1171..1190)
/label=OL2256
primer_bind complement(1172..1193)
/label=OL1702
CDS 1191..1787
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(1191..1210)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 1194..1213
/label=OL2264
primer_bind 1687..1707
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
primer_bind 1687..1706
/label=OL2294
primer_bind 1687..1705
/label=OL2339
primer_bind 1758..1812
/label=OL901
primer_bind complement(1764..1787)
/label=OL306
primer_bind complement(1773..1795)
/label=OL384
primer_bind 1780..1811
/label=OL379
CDS 1788..1844
/codon_start=1
/product="2A peptide from porcine teschovirus-1
polyprotein"
/label=P2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="ATNFSLLKQAGDVEENPGP"
primer_bind 1788..1844
/label=OL866
primer_bind complement(1788..1844)
/label=OL867
primer_bind 1788..1814
/label=OL2251
primer_bind 1825..1868
/label=OL902
primer_bind complement(1825..1852)
/label=OL966
RBS 1827..1834
/note="strong bacterial ribosome binding site (Elowitz and
Leibler, 2000)"
CDS 1845..2522
/codon_start=1
/product="monomeric derivative of DsRed (Campbell et al.,
2002)"
/label=mRFP1
/translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
TGA"
primer_bind 1845..1863
/label=OL2300
primer_bind 2076..2095
/label=OL1123
primer_bind complement(2085..2111)
/label=OL1728
primer_bind complement(2117..2143)
/label=OL2455
primer_bind complement(2500..2547)
/label=OL903
primer_bind complement(2500..2522)
/label=OL2353
primer_bind complement(2501..2522)
/label=OL2708
primer_bind 2521..2539
/label=OL965
primer_bind 2523..2545
/label=OL305
polyA_signal 2669..2790
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind 2669..2705
/label=OL1698
primer_bind complement(2680..2699)
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind 2734..2753
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind complement(2774..2806)
/label=OL2209
primer_bind 2791..2824
/label=OL1692
primer_bind complement(2804..2830)
/label=OL1941
primer_bind complement(2979..3002)
/label=OL1043
primer_bind 2980..3002
/label=OL1044
primer_bind complement(3038..3055)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(3209..3797)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(3289..3308)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind complement(3391..3410)
/label=OL1041
primer_bind complement(3524..3543)
/label=OL631
primer_bind 3580..3599
/label=OL798
primer_bind complement(3821..3860)
/label=OL660
primer_bind 3841..3860
/label=OL659
CDS complement(3968..4828)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 4425..4444
/label=OL1922
primer_bind 4591..4610
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
primer_bind complement(4591..4610)
/label=OL1923
primer_bind 4744..4763
/label=OL1033
primer_bind complement(4744..4763)
/label=OL1034
promoter complement(4829..4933)
/gene="bla"
/label=AmpR promoter
rep_origin 4960..5415
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(4985..4994)
/label=OL305
primer_bind complement(5047..5066)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 5257..5278
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 5468..5487
/label=OL1469
primer_bind 5477..5493
/label=OL1109
polyA_signal 5546..5594
/note="synthetic polyadenylation signal"
misc_feature 5608..5699
/label=pause site
/note="RNA polymerase II transcriptional pause signal from
the human alpha-2 globin gene"
primer_bind 5608..5624
/label=OL1167
primer_bind 5654..5673
/label=OL1087
primer_bind 5678..5692
/label=OL1935
primer_bind join(5708..5716,1..17)
/label=OL1700
ORIGIN
1 agtgaacgga tctcgacggt atcgatcacg agactagcct agagggggta tagctcagtg
61 gtagagcatt tgactgcaga tcaagaggtc cccggttcaa atccgggtgc cccctccccc
121 cccctccgat ggcaggtttt agagctagaa atagcaagtt aaaataaggc tagtccgtta
181 tcaacttgaa aaagtggcac cgagtcggtg cagagggggt atagctcagt ggtagagcat
241 ttgactgcag atcaagaggt ccccggttca aatccgggtg ccccctactg ccatcggagg
301 gggggggttt tagagctaga aatagcaagt taaaataagg ctagtccgtt atcaacttga
361 aaaagtggca ccgagtcggt gcacccgggc cacccccccc ctccactttc tccgcggttc
421 tatctagtta cgcgttaaac caactagaat ttttttctcg acctcgagac aaatggcagt
481 attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
541 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat
601 tcaaaatttt cgggtttatt acagggacag cagagatcca ctttggccgc ggctcgaggg
661 ggttggggtt gcgccttttc caaggcagcc ctgggtttgc gcagggacgc ggctgctctg
721 ggcgtggttc cgggaaacgc agcggcgccg accctgggac tcgcacattc ttcacgtccg
781 ttcgcagcgt cacccggatc ttcgccgcta cccttgtggg ccccccggcg acgcttcctg
841 ctccgcccct aagtcgggaa ggttccttgc ggttcgcggc gtgccggacg tgacaaacgg
901 aagccgcacg tctcactagt accctcgcag acggacagcg ccagggagca atggcagcgc
961 gccgaccgcg atgggctgtg gccaatagcg gctgctcagc agggcgcgcc gagagcagcg
1021 gccgggaagg ggcggtgcgg gaggcggggt gtggggcggt agtgtgggcc ctgttcctgc
1081 ccgcgcggtg ttccgcattc tgcaagcctc cggagcgcac gtcggcagtc ggctccctcg
1141 ttgaccgaat caccgacctc tctccccagg gggatccacc ggagcttacc atgaccgagt
1201 acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg
1261 ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg
1321 agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg
1381 tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag
1441 cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg
1501 ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt
1561 tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg
1621 tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gaaacctccg
1681 cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg
1741 tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgccgct actaacttca
1801 gcctgctgaa gcaggctgga gacgtggagg agaaccctgg acctatggcg agtagcgaag
1861 acgttatcaa agagttcatg cgtttcaaag ttcgtatgga aggttccgtt aacggtcacg
1921 agttcgaaat cgaaggtgaa ggtgaaggtc gtccgtacga aggtacccag accgctaaac
1981 tgaaagttac caaaggtggt ccgctgccgt tcgcttggga catcctgtcc ccgcagttcc
2041 agtacggttc caaagcttac gttaaacacc cggctgacat cccggactac ctgaaactgt
2101 ccttcccgga aggtttcaaa tgggaacgtg ttatgaactt cgaagacggt ggtgttgtta
2161 ccgttaccca ggactcctcc ctgcaagacg gtgagttcat ctacaaagtt aaactgcgtg
2221 gtaccaactt cccgtccgac ggtccggtta tgcagaaaaa aaccatgggt tgggaagctt
2281 ccaccgaacg tatgtacccg gaagacggtg ctctgaaagg tgaaatcaaa atgcgtctga
2341 aactgaaaga cggtggtcac tacgacgctg aagttaaaac cacctacatg gctaaaaaac
2401 cggttcagct gccgggtgct tacaaaaccg acatcaaact ggacatcacc tcccacaacg
2461 aagactacac catcgttgaa cagtacgaac gtgctgaagg tcgtcactcc accggtgctt
2521 aacgcccgcc ccacgacccg cagcgcccga ccgaaaggag cgcacgaccc catgcatcgg
2581 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ctagagtcgg
2641 ggcggccggc cgcttcgagc agacatgata agatacattg atgagtttgg acaaaccaca
2701 actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt
2761 gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt
2821 caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt
2881 aaaatcgata aggatccgtc gaccgatgcc cttgagagcc ttcaacccag tcagctcctt
2941 ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg actgtcttct ttatcatgca
3001 actcgtagga caggtgccgg cagcgctctt ccgcttcctc gctcactgac tcgctgcgct
3061 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
3121 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
3181 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
3241 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
3301 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
3361 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
3421 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
3481 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
3541 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
3601 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
3661 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
3721 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
3781 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
3841 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
3901 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
3961 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
4021 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
4081 ctggccccag tgctgcaatg ataccgcggg acccacgctc accggctcca gatttatcag
4141 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
4201 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
4261 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
4321 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
4381 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
4441 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
4501 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
4561 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
4621 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
4681 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
4741 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
4801 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
4861 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
4921 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgcgccctgt agcggcgcat
4981 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
5041 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
5101 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
5161 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
5221 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
5281 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
5341 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
5401 taacgcttac aatttgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg
5461 tgcgggcctc ttcgctatta cgccagccca agctaccatg ataagtaagt aatattaagg
5521 tacgggaggt acttggagcg gccgcaataa aatatcttta ttttcattac atctgtgtgt
5581 tggttttttg tgtgaatcga tagtactaac atacgctctc catcaaaaca aaacgaaaca
5641 aaacaaacta gcaaaatagg ctgtccccag tgcaagtgca ggtgccagaa catttctcta
5701 tcgataggta ccgatt
//
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