| 质粒名称: | pHR-tdPP7-3xmCherry |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 74926 |
| 存储实验室: | Marvin Tanenbaum |
|
载体骨架基本信息 |
|
| 载体名称: | pHR |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，慢病毒 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | PP7-3xmCherry |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pHR-tdPP7-3xmCherry was a gift from Marvin Tanenbaum (Addgene plasmid # 74926 ; http://n2t.net/addgene:74926 ; RRID:Addgene_74926) |
| 参考文献部分: | Dynamics of Translation of Single mRNA Molecules In Vivo. Yan X, Hoek TA, Vale RD, Tanenbaum ME. Cell. 2016 May 5;165(4):976-89. doi: 10.1016/j.cell.2016.04.034. 10.1016/j.cell.2016.04.034 PubMed 27153498 |
买家导航
pHR-tdPP7-3xmCherry载体质粒基本信息
pHR-tdPP7-3xmCherry质粒图谱载体图谱和pHR-tdPP7-3xmCherry载体序列质粒序列多克隆位点信息
pHR-tdPP7-3xmCherry质粒载体简介
pHR-tdPP7-3xmCherry质粒序列载体序列
LOCUS pHR-tdPP7-3xmCherry 11752 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Expresses tdPP7-3xmCherry.
ACCESSION .
VERSION .
KEYWORDS pHR-tdPP7-3xmCherry
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11752)
AUTHORS Yan X, Hoek TA, Vale RD, Tanenbaum ME
TITLE Dynamics of Translation of Single mRNA Molecules In Vivo.
JOURNAL Cell. 2016 May 5;165(4):976-89. doi: 10.1016/j.cell.2016.04.034.
PUBMED 27153498
REFERENCE 2 (bases 1 to 11752)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..11752
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(302..321)
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
primer_bind 655..677
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(715..733)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 801..905
/gene="bla"
/label=AmpR promoter
CDS 906..1766
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(1124..1143)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 1937..2525
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 2426..2445
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 2679..2696
/label=L4440
/note="L4440 vector, forward primer"
primer_bind complement(2766..2786)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 2771..3100
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 2951..3086
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 3013..3032
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
intron 4234..4299
/label=small t intron
/note="SV40 (simian virus 40) small t antigen intron"
CDS 4429..4449
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
polyA_signal 4874..5008
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(4911..4930)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 4965..4984
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
LTR 5177..5810
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
misc_feature 5857..5982
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 6474..6707
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 6892..6936
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 7221..7338
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 7491..7898
/label=SFFV promoter
/note="spleen focus-forming virus long terminal repeat
(LTR) promoter"
CDS 8797..9501
/codon_start=1
/product="monomeric derivative of DsRed fluorescent protein
(Shaner et al., 2004)"
/label=mCherry
/note="mammalian codon-optimized"
/translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
GRHSTGGMDELYK"
CDS 9547..9708
/codon_start=1
/product="B1 domain of Streptococcal protein G "
/label=GB1
/note="effective as a solubilizing fusion partner (Cheng
and Patel, 2004)"
/translation="YKLILNGKTLKGETTTEAVDAATAEKVFKQYANDNGVDGEWTYDD
ATKTFTVTE"
CDS 9733..10437
/codon_start=1
/product="monomeric derivative of DsRed fluorescent protein
(Shaner et al., 2004)"
/label=mCherry
/note="mammalian codon-optimized"
/translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
GRHSTGGMDELYK"
CDS 10483..10644
/codon_start=1
/product="B1 domain of Streptococcal protein G "
/label=GB1
/note="effective as a solubilizing fusion partner (Cheng
and Patel, 2004)"
/translation="YKLILNGKTLKGETTTEAVDAATAEKVFKQYANDNGVDGEWTYDD
ATKTFTVTE"
CDS 10702..11406
/codon_start=1
/product="monomeric derivative of DsRed fluorescent protein
(Shaner et al., 2004)"
/label=mCherry
/note="mammalian codon-optimized"
/translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
GRHSTGGMDELYK"
LTR 11488..11721
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
promoter complement(11746..12)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(11747..12)
/label=SP6
/note="SP6 promoter, forward primer"
ORIGIN
1 tgtcacctaa atcgtatgtg tatgatacat aaggttatgt attaattgta gccgcgttct
61 aacgacaata tgtacaagcc taattgtgta gcatctggct tactgaagca gaccctatca
121 tctctctcgt aaactgccgt cagagtcggt ttggttggac gaaccttctg agtttctggt
181 aacgccgtcc cgcacccgga aatggtcagc gaaccaatca gcagggtcat cgctagccag
241 atcctctacg ccggacgcat cgtggccggc atcaccggcg ccacaggtgc ggttgctggc
301 gcctatatcg ccgacatcac cgatggggaa gatcgggctc gccacttcgg gctcatgagc
361 gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt gggcgccatc
421 tccttgcatg caccattcct tgcggcggcg gtgctcaacg gcctcaacct actactgggc
481 tgcttcctaa tgcaggagtc gcataaggga gagcgtcgaa tggtgcactc tcagtacaat
541 ctagctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc
601 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
661 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg
721 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
781 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
841 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
901 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
961 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
1021 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
1081 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
1141 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
1201 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
1261 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
1321 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
1381 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
1441 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
1501 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
1561 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
1621 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
1681 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
1741 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
1801 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
1861 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
1921 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
1981 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
2041 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
2101 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
2161 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
2221 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
2281 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
2341 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
2401 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
2461 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
2521 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
2581 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
2641 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
2701 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
2761 gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
2821 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
2881 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
2941 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
3001 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
3061 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagcttggac acaagacagg
3121 cttgcgagat atgtttgaga ataccacttt atcccgcgtc agggagaggc agtgcgtaaa
3181 aagacgcgga ctcatgtgaa atactggttt ttagtgcgcc agatctctat aatctcgcgc
3241 aacctatttt cccctcgaac actttttaag ccgtagataa acaggctggg acacttcaca
3301 tgagcgaaaa atacatcgtc acctgggaca tgttgcagat ccatgcacgt aaactcgcaa
3361 gccgactgat gccttctgaa caatggaaag gcattattgc cgtaagccgt ggcggtctgt
3421 accgggtgcg ttactggcgc gtgaactggg tattcgtcat gtcgataccg tttgtatttc
3481 cagctacgat cacgacaacc agcgcgagct taaagtgctg aaacgcgcag aaggcgatgg
3541 cgaaggcttc atcgttattg atgacctggt ggataccggt ggtactgcgg ttgcgattcg
3601 tgaaatgtat ccaaaagcgc actttgtcac catcttcgca aaaccggctg gtcgtccgct
3661 ggttgatgac tatgttgttg atatcccgca agatacctgg attgaacagc cgtgggatat
3721 gggcgtcgta ttcgtcccgc caatctccgg tcgctaatct tttcaacgcc tggcactgcc
3781 gggcgttgtt ctttttaact tcaggcgggt tacaatagtt tccagtaagt attctggagg
3841 ctgcatccat gacacaggca aacctgagcg aaaccctgtt caaaccccgc tttaaacatc
3901 ctgaaacctc gacgctagtc cgccgcttta atcacggcgc acaaccgcct gtgcagtcgg
3961 cccttgatgg taaaaccatc cctcactggt atcgcatgat taaccgtctg atgtggatct
4021 ggcgcggcat tgacccacgc gaaatcctcg acgtccaggc acgtattgtg atgagcgatg
4081 ccgaacgtac cgacgatgat ttatacgata cggtgattgg ctaccgtggc ggcaactgga
4141 tttatgagtg ggccccggat ctttgtgaag gaaccttact tctgtggtgt gacataattg
4201 gacaaactac ctacagagat ttaaagctct aaggtaaata taaaattttt aagtgtataa
4261 tgtgttaaac tactgattct aattgtttgt gtattttaga ttccaaccta tggaactgat
4321 gaatgggagc agtggtggaa tgcctttaat gaggaaaacc tgttttgctc agaagaaatg
4381 ccatctagtg atgatgaggc tactgctgac tctcaacatt ctactcctcc aaaaaagaag
4441 agaaaggtag aagaccccaa ggactttcct tcagaattgc taagtttttt gagtcatgct
4501 gtgtttagta atagaactct tgcttgcttt gctatttaca ccacaaagga aaaagctgca
4561 ctgctataca agaaaattat ggaaaaatat tctgtaacct ttataagtag gcataacagt
4621 tataatcata acatactgtt ttttcttact ccacacaggc atagagtgtc tgctattaat
4681 aactatgctc aaaaattgtg tacctttagc tttttaattt gtaaaggggt taataaggaa
4741 tatttgatgt atagtgcctt gactagagat cataatcagc cataccacat ttgtagaggt
4801 tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc
4861 aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
4921 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
4981 catcaatgta tcttatcatg tctggatcaa ctggataact caagctaacc aaaatcatcc
5041 caaacttccc accccatacc ctattaccac tgccaattac ctagtggttt catttactct
5101 aaacctgtga ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt taaatatgta
5161 ctacaaactt agtagttgga agggctaatt cactcccaaa gaagacaaga tatccttgat
5221 ctgtggatct accacacaca aggctacttc cctgattagc agaactacac accagggcca
5281 ggggtcagat atccactgac ctttggatgg tgctacaagc tagtaccagt tgagccagat
5341 aaggtagaag aggccaataa aggagagaac accagcttgt tacaccctgt gagcctgcat
5401 gggatggatg acccggagag agaagtgtta gagtggaggt ttgacagccg cctagcattt
5461 catcacgtgg cccgagagct gcatccggag tacttcaaga actgctgata tcgagcttgc
5521 tacaagggac tttccgctgg ggactttcca gggaggcgtg gcctgggcgg gactggggag
5581 tggcgagccc tcagatcctg catataagca gctgcttttt gcctgtactg ggtctctctg
5641 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
5701 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
5761 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
5821 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
5881 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
5941 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
6001 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
6061 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
6121 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
6181 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
6241 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
6301 aagaccaccg cacagcaagc ggccggtgat cttcagacct ggacgatata tatgagggac
6361 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
6421 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
6481 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
6541 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
6601 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
6661 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
6721 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
6781 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
6841 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
6901 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
6961 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
7021 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
7081 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
7141 ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg gtatcgccaa
7201 atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg
7261 gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt
7321 acaaaaattc aaaattttcg ggtttattac agggacagca gagatccagt ttggatcgat
7381 aagcttgata tcgaattcct gcagccccga taaaataaaa gattttattt agtctccaga
7441 aaaagggggg aatgaaagac cccacctgta ggtttggcaa gctagctgca gtaacgccat
7501 tttgcaaggc atggaaaaat accaaaccaa gaatagagaa gttcagatca agggcgggta
7561 catgaaaata gctaacgttg ggccaaacag gatatctgcg gtgagcagtt tcggccccgg
7621 cccggggcca agaacagatg gtcaccgcag tttcggcccc ggcccgaggc caagaacaga
7681 tggtccccag atatggccca accctcagca gtttcttaag acccatcaga tgtttccagg
7741 ctcccccaag gacctgaaat gaccctgcgc cttatttgaa ttaaccaatc agcctgcttc
7801 tcgcttctgt tcgcgcgctt ctgcttcccg agctctataa aagagctcac aacccctcac
7861 tcggcgcgcc agtcctccga cagactgagt cgcccggggg ggatctggag ctctcgagaa
7921 ttctcacgcg ccggatccgc caccatgtcc aaaaccatcg ttctttcggt cggcgaggct
7981 actcgcactc tgactgagat ccagtccacc gcagaccgtc agatcttcga agagaaggtc
8041 gggcctctgg tgggtcggct gcgcctcacg gcttcgctcc gtcaaaacgg agccaagacc
8101 gcgtatcgcg tcaacctaaa actggatcag gcggacgtcg ttgattgctc caccagcgtc
8161 tgcggcgagc ttccgaaagt gcgctacact caggtatggt cgcacgacgt gacaatcgtt
8221 gcgaatagca ccgaggcctc gcgcaaatcg ttgtacgatt tgaccaagtc cctcgtcgcg
8281 acctcgcagg tcgaagatct tgtcgtcaac cttgtgccgc tgggccgtcg tgcggacccg
8341 ctagcctcct gcggccgctc caaaaccatc gttctttcgg tcggcgaggc tactcgcact
8401 ctgactgaga tccagtccac cgcagaccgt cagatcttcg aagagaaggt cgggcctctg
8461 gtgggtcggc tgcgcctcac ggcttcgctc cgtcaaaacg gagccaagac cgcgtatcgc
8521 gtcaacctaa aactggatca ggcggacgtc gttgattgct ccaccagcgt ctgcggcgag
8581 cttccgaaag tgcgctacac tcaggtatgg tcgcacgacg tgacaatcgt tgcgaatagc
8641 accgaggcct cgcgcaaatc gttgtacgat ttgaccaagt ccctcgtcgc gacctcgcag
8701 gtcgaagatc ttgtcgtcaa ccttgtgccg ctgggccgtc gtgcggaccc gctagcctcc
8761 acgcgtgatt cgactagtgg aggaagcgga ggaggagtga gcaagggcga ggaggataac
8821 atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc
8881 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc
8941 aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag
9001 ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag
9061 ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg
9121 gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg
9181 cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag
9241 gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg
9301 ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag
9361 aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac
9421 aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc
9481 ggcatggacg agctgtacaa gcatggcatg gatgagctct acaaaggtgg aggtcggacc
9541 gaagagtaca agcttatcct gaacggtaaa accctgaaag gtgaaaccac caccgaagct
9601 gttgacgctg ctaccgcgga aaaagttttc aaacagtacg ctaacgacaa cggtgttgac
9661 ggtgaatgga cctacgacga cgctaccaaa accttcacgg taaccgaagc tagtggagga
9721 agcggaggag gagtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg
9781 cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag
9841 ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc
9901 cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac
9961 gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag
10021 tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc
10081 ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac
10141 ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc
10201 gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac
10261 tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc
10321 tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa
10381 cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaagcat
10441 ggcatggatg agctctacaa aggtggaggt cggaccgaag agtacaagct tatcctgaac
10501 ggtaaaaccc tgaaaggtga aaccaccacc gaagctgttg acgctgctac cgcggaaaaa
10561 gttttcaaac agtacgctaa cgacaacggt gttgacggtg aatggaccta cgacgacgct
10621 accaaaacct tcacggtaac cgaagctagt ggtggtggtg gtagcggtgg tggtagcagc
10681 ttaccggtcg ccgaattcgg tgtgagcaag ggcgaggagg ataacatggc catcatcaag
10741 gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc
10801 gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc
10861 aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc
10921 aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag
10981 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag
11041 gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc
11101 ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg
11161 atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac
11221 ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg
11281 cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc
11341 atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg
11401 tacaagcata tgtaagcggc cctttaagac caatgactta caaggcagct gtagatctta
11461 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag
11521 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct
11581 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
11641 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
11701 cagtgtggaa aatctctagc agcatctaga attaattccg tgtattctat ag
//
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