| 质粒名称: | pSF11-wmiRFP670-2-T2A-HO1 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000106 |
| 产品用途: | Expresses miRFP670-2 in C. elegans neurons |
| 存储实验室: | KIRYL PIATKEVICH Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pSF11 |
| 表达系统: | 线虫表达 |
| 启动子: |
tag168
|
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a，TOP10 |
|
插入基因信息 |
|
| 插入基因名称: | miRFP670-2-T2A-HO1 |
| 物种来源: | Synthetic, Homo sapiens |
| 密码子优化: | 蠕虫表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Quantitative assessment of near-infrared fluorescent proteins
Zhang H., Papadaki S., Sun X., Wang X., Drobizhev M., Yao L., Rehbock M., Köster R.W., Wu L., Namikawa K., Piatkevich K.D.
Nat. Methods 2023 2010 20, 1605–1616 (2023)
|
买家导航
pSF11-wmiRFP670-2-T2A-HO1载体质粒基本信息
pSF11-wmiRFP670-2-T2A-HO1质粒图谱载体图谱和pSF11-wmiRFP670-2-T2A-HO1载体序列质粒序列多克隆位点信息
pSF11-wmiRFP670-2-T2A-HO1质粒载体简介
pSF11-wmiRFP670-2-T2A-HO1质粒序列载体序列
LOCUS pSF11-wmiRFP670-2-T2A-HO1 8224 bp DNA circular SYN 21-APR-2023
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8224)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 23, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..8224
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 54..2717
/label=tag-168 promoter
primer_bind 2796..2816
/label=pSF11-Fw-Seq
primer_bind 2808..2861
/label=0914-693-F
misc_feature 2834..2837
/label=optimized sequence for ribosomal binding site
misc_feature 2834..2837
/label=codon adapted insert for smGFP-myc
/note="codon adapted by
https://worm.mpi-cbg.de/codons/cgi-bin/optimize.py
Intron * 2 (3 requested, but 1 is inside myc regions and
thus removed)
CAI set to 0.60
Check all 3 boxes
"
CDS 2838..3872
/codon_start=1
/label=wmiRFP670-2
/translation="MARKVDLTSCDREPIHIPGSIQPCGCLLACDAQAVRITRITENAG
AFFGRETPRVGELLADYFGETEAHALRNALAQSSDPKRPALIFGWRDGLTGRTFDISLH
RHDGTSIIEFEPAAAEQADNPLRLTRQIIARTKVSLNIYILTNPDYLNFQELKSLEEMA
ARVPRYLQAMLGYHRVMLYRFADDGSGMVIGEAKRSDLESFLGQHFPASLVPQQARLLY
LKVSLNSSVLTNHTYLNFQNAIRVVSDSRGISSRIVPEHDASGAALDLSFAHLRSISPC
HLEFLRNMGVSASMSLSIIIDGTLWGLIICHHYEPRAVPMAQRVAAKRFAERLSTHFTA
AHHQR"
misc_feature 3249..3299
/label=intron-1
misc_feature 3510..3560
/label=intron-2
CDS 3873..3893
/codon_start=1
/label=Linker
/translation="GYDIGSG"
CDS 3894..3947
/codon_start=1
/product="2A peptide from Thosea asigna virus capsid
protein"
/label=T2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="EGRGSLLTCGDVEENPGP"
CDS 3948..4967
/codon_start=1
/label=HO1
/translation="MERPQPDSMPQDLSEALKEATKEVHTQAENAEFMRNFQKGQVTRD
GFKLVMASLYHIYVALEEEIERNKVSLNIYILTNPDYLNFQESPVFAPVYFPEELHRKA
ALEQDLAFWYGPRWQEVIPYTPAMQRYVKRLHEVGRTEPELLVAHAYTRYLGDLSGGQV
LKVSLNSSVLTNHTYLNFQKIAQKALDLPSSGEGLAFFTFPNIASATKFKQLYRSRMNS
LEMTPAVRQRVIEEAKVSLNMILLTN*SDLNFQTAFLLNIQLFEELQELLTHDTKDQSP
SRAPGLRQRASNKVQDSAPVETPRGKPPLNTRSQAPLLRWVLTLSFLVATVAVGLYAM"
misc_feature 4155..4205
/label=Intron-1
primer_bind complement(4220..4242)
/label=wHO1-R
misc_feature 4443..4493
/label=Intron-2
misc_feature 4662..4712
/label=Intron-3
primer_bind complement(4944..4993)
/label=0914-693-R
primer_bind complement(5009..5025)
/label=pSF11-Rv-Seq
promoter 6103..6207
/gene="bla"
/label=AmpR promoter
CDS 6208..7068
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 7163..7845
/label=ColE1 origin
protein_bind 8115..8136
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 8151..8181
/label=lac promoter
/note="promoter for the E. coli lac operon"
misc_binding 8185..8207
/label=LacO
protein_bind 8189..8205
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind join(8213..8224,1..5)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
ORIGIN
1 atgaccatga ttacgccaag cttgcatgcg cggccgcact gactgggccg gccggatcca
61 taggtatgcc tgtcattctc tatttttaaa cgttcctgca gcagtagcac aacagagcca
121 gcagttttcc tcttcttctc catcttcttc tgcttcttcc tctcttccca aaaaaaaaaa
181 aaaaaaaaaa gtgagggggc tcactgcccc cccccccctc ccccccccct cttctccttg
241 aagctcatcc agacgtcgct gtcctcctcc tcctcgtccc cggggcgctg tgtgtgtgtg
301 tgtgtgctcc gtgagcatgt agcagcttca gcccatttaa aggcgcacac tctctgcatc
361 tctgcgtgtc tcgacactcg ttttttctct tcttttctgc tgcacacaca cacacacaca
421 cacacacgct catgccagag cacttcttgc tccggggggc tccgcgcgga gcccgagagg
481 acctcctcct cctttttctt cttcgctttt tagttcttct tgcctcggtc tcgccccttg
541 ttttgctctc catgtagtat atacatctgg gtgtgtgtgt gtgtgtgcac ctttggagca
601 ttttttgaac ttttccagaa ctttggagca acattttcag cttgttcagt gcacttttta
661 ggctgaaaat cctattttta ggttttcgaa agtttttttg gagcattttt cgtgcaattt
721 aggtgcaaaa ttaaaaaaaa gggttttgta gtgaattaaa atttttttga aactttcgcc
781 tttttaaaaa aatgtttcta atatctttaa aaattggaat attttggaaa acaatttgtt
841 ttcaattggc aatttattaa ttctgagctt ctgagcgtaa attttttttt attttggagc
901 aattctgaga tgtcttagtg catttttcgg gcatacataa aaaaaaattt atcttaataa
961 taaaaacctt aaatttaggc ttcaccgtct ggaaattcaa ttttttcagc tgaaaaactt
1021 tcaaaaaaaa aaaaattaaa ttttcacatt tattttctct ggaattttgg agcaatttca
1081 aagctgtctt agtgcatttt tagtacacaa atattttttc atttttctta aattttaata
1141 atctaagctt aaataaaggc ttcaccgtct gaaaatgcaa cttttttcag atgaaatatt
1201 aaaaaaaaaa acctctttta ttaattttgt gctccaaact ttcccacggt acgcaaatca
1261 tgtaatatgt acttttaaac attatttttc caatcaaaaa tatccaacag cttttcctta
1321 caatttgaaa atttttatta aaatttcaaa aaaatttttt ttttttttta atttcgaatt
1381 tttttaaact tgttgtaact tttttccctg tcggcgccaa ttaacaaaat acgtagaatt
1441 ttgcgcttat tgttttgtta gttaacaaat ttttgacaac tttcttatca gctgagattt
1501 aggtgtttca gttcgcaatc tgcgtctctc cttgctctcc tctattttaa aacccccata
1561 actttgttct tgtgagagtt atcaaaaaat tgtcaactaa caaaatatag agcacaaaaa
1621 tatgcacatt ttgtatgttg acaatttttt gatacctttt ttagttttcg agatatctta
1681 gttttagttt agctgtacct ttttctggca aaaaacttcc aaatttcggc aatttttcgg
1741 caaatttccc ggcaaatttc gccaagtaca tcgggctctc agacaagtgg tctgtgagtg
1801 gaagtcgata aaagtagttt acgtgctctc cgtccccccg cttcttctcc gtctctgctt
1861 ctatttctcc attgactagt cccactactt tttacggggc ggagggctct cttgcactcc
1921 agcttcttct ctttttgtgc tcttttttgc ccgtatgtgg gtgctcattt atttttcttt
1981 ggttttaaaa taaattcggt ttattaggct actcgtaaaa atgaagaaaa aacttcaaat
2041 tcaaacgaca ttaaaaaaaa atttaataaa aattttttga attgctcaaa agccccacct
2101 atatatatat atagctccgc ctacttctcg cacagacccc gcccactttt tgactccgcc
2161 cccttattgg caaaagttct tgaaaaaaat aaaaataaaa attcaaattg aaacaatttt
2221 tttttaatga agtagttttt ttctaaattg atgaaaattt ctaaacagtt tacaagcacc
2281 gcccactttt tgactccgcc cctttgttgg aaaaattctt gtaaaaaaaa ataaaaaata
2341 aaagcttctc tttcaaacga tttttagttg gtttttgtat gaaaagtttt tttcctaaaa
2401 tagaaatgtc tgaataattt gcaagctccg cccttttctt acccagactc cgccccttta
2461 ttgactaatt tttttttgtc catttacctg aagttcaaaa aaaaaattta cagtttcata
2521 gagtaataat ttttcaaaaa tctctttttc caatgtttaa tgtgtcatta taaacccgcc
2581 cagttttaag ctccgcccat ttaggccccg cccatttatg acccaaatat ttttttctct
2641 attcacccgt ggatttccct ccaacaagtt accccaaaaa taaaaataaa aattcctgca
2701 ggcgcccacc gggatccggc gcgcctctag aggatccccg ggattggcca aaggacccaa
2761 aggtatgttt cgaatgatac taacataaca tagaacattt tcaggaggac ccttggctag
2821 cgtcgacggt accaaaaatg gcccgtaagg tcgaccttac ctcctgcgac cgcgagccaa
2881 tccacatccc aggatccatc cagccatgcg gatgccttct tgcctgcgac gcccaagccg
2941 tccgcatcac ccgcatcacc gagaacgccg gagccttctt cggacgcgag accccacgtg
3001 tcggagagct tcttgccgac tacttcggag agaccgaggc ccacgccctt cgtaacgccc
3061 ttgcccaatc ctccgaccca aagcgtccag cccttatctt cggatggcgc gacggactta
3121 ccggacgcac cttcgacatc tcccttcacc gtcacgacgg aacctccatc atcgagttcg
3181 agccagccgc cgccgagcaa gccgacaacc cacttcgcct tacccgccaa atcatcgccc
3241 gcaccaaggt aagtttaaac atatatatac taactaaccc tgattattta aattttcagg
3301 agcttaagtc ccttgaggag atggccgccc gtgtcccacg ttaccttcaa gccatgcttg
3361 gataccaccg tgtcatgctt taccgtttcg ccgacgacgg atccggaatg gtcatcggag
3421 aggccaagcg ttccgacctt gagtccttcc ttggacaaca cttcccagcc tcccttgtcc
3481 cacaacaagc ccgtcttctt taccttaagg taagtttaaa cagttcggta ctaactaacc
3541 atacatattt aaattttcag aacgccatcc gtgtcgtctc cgactcccgt ggaatctcct
3601 cccgtatcgt cccagagcac gacgcctccg gagccgccct tgacctttcc ttcgcccacc
3661 ttcgttccat ctccccatgc caccttgagt tccttcgcaa catgggagtc tccgcctcca
3721 tgtccctttc catcatcatc gacggaaccc tttggggact tatcatctgc caccactacg
3781 agccacgtgc cgtcccaatg gcccaacgtg tcgccgccaa gcgtttcgcc gagcgtcttt
3841 ccacccactt caccgccgcc caccaccaac gtggatacga catcggatcc ggagagggac
3901 gcggatccct tcttacctgc ggagatgttg aggaaaaccc aggaccaatg gagcgcccac
3961 aaccagattc tatgccacaa gacctttccg aggcccttaa ggaggctacc aaggaggttc
4021 acacccaagc tgagaacgct gagttcatgc gcaacttcca aaagggacaa gttacccgtg
4081 atggattcaa gcttgtcatg gcttccctct accacatcta cgtcgctctt gaggaggaga
4141 tcgagcgcaa caaggtaagt ttaaacatat atatactaac taaccctgat tatttaaatt
4201 ttcaggagtc tccagttttc gctccagttt acttcccaga agagctccac cgtaaggctg
4261 cccttgagca agaccttgcc ttctggtatg gaccacgctg gcaagaagtt atcccataca
4321 ctccagctat gcaacgctac gttaagcgtc ttcacgaggt cggacgtacc gagccagagc
4381 ttcttgtcgc tcacgcttac acccgttacc ttggagacct ttccggagga caagtcctta
4441 aggtaagttt aaacagttcg gtactaacta accatacata tttaaatttt cagaagatcg
4501 ctcaaaaggc tcttgacctt ccatcttccg gagagggact tgccttcttc actttcccaa
4561 acatcgcttc tgccactaag ttcaagcaac tttaccgctc tcgtatgaac tctcttgaga
4621 tgaccccagc tgtccgccaa cgtgtcatcg aggaggctaa ggtaagttta aacatgattt
4681 tactaactaa ctaatctgat ttaaattttc agaccgcttt ccttcttaac atccaacttt
4741 tcgaagagct tcaagagctt cttacccacg acaccaagga ccaatctcca tcccgtgccc
4801 caggacttcg tcaacgtgcc tccaacaagg tccaagattc cgctccagtt gagactccac
4861 gtggaaagcc accacttaac acccgttccc aagccccact tcttcgttgg gtccttaccc
4921 tctccttcct tgtcgccacc gtcgctgtcg gactttacgc catgtaagag ctccgcatcg
4981 gccgctgtca tcagatcgcc atctcgcgcc cgtgcctctg acttctaagt ccaattactc
5041 ttcaacatcc ctacatgctc tttctccctg tgctcccacc ccctattttt gttattatca
5101 aaaaaacttc ttcttaattt ctttgttttt tagcttcttt taagtcacct ctaacaatga
5161 aattgtgtag attcaaaaat agaattaatt cgtaataaaa agtcgaaaaa aattgtgctc
5221 cctcccccca ttaataataa ttctatccca aaatctacac aatgttctgt gtacacttct
5281 tatgtttttt ttacttctga taaatttttt ttgaaacatc atagaaaaaa ccgcacacaa
5341 aataccttat catatgttac gtttcagttt atgaccgcaa tttttatttc ttcgcacgtc
5401 tgggcctctc atgacgtcaa atcatgctca tcgtgaaaaa gttttggagt atttttggaa
5461 tttttcaatc aagtgaaagt ttatgaaatt aattttcctg cttttgcttt ttgggggttt
5521 cccctattgt ttgtcaagag tttcgaggac ggcgtttttc ttgctaaaat cacaagtatt
5581 gatgagcacg atgcaagaaa gatcggaaga aggtttgggt ttgaggctca gtggaaggtg
5641 agtagaagtt gataatttga aagtggagta gtgtctatgg ggtttttgcc ttaaatgaca
5701 gaatacattc ccaatatacc aaacataact gtttcctact agtcggccgt acgggccctt
5761 tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
5821 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
5881 gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag
5941 agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
6001 gcggccttaa ttaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
6061 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
6121 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
6181 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
6241 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
6301 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
6361 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
6421 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
6481 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
6541 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
6601 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
6661 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
6721 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
6781 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
6841 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
6901 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
6961 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
7021 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
7081 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
7141 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
7201 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
7261 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
7321 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
7381 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
7441 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
7501 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
7561 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
7621 cagcgtgagc attgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
7681 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
7741 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
7801 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
7861 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
7921 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
7981 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
8041 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
8101 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
8161 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
8221 agct
//
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