| 质粒名称: |
pAAVLINK-EF1alpha-5'-NEO1
|
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001844 |
| 产品用途: | 5' AAVLINK plasmid for NEO1 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | EF1alpha |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | NEO1 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-EF1alpha-5'-NEO1
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-EF1alpha-5'-NEO1载体质粒基本信息
pAAVLINK-EF1alpha-5'-NEO1质粒图谱载体图谱和pAAVLINK-EF1alpha-5'-NEO1载体序列质粒序列多克隆位点信息
pAAVLINK-EF1alpha-5'-NEO1质粒载体简介
pAAVLINK-EF1alpha-5'-NEO1质粒序列载体序列
LOCUS pAAVLINK-EF1alpha-5'-NEO1 5664 bp DNA circular SYN 10-AUG-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5664)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 10, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5664
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
/note="color: #ffe4c4"
promoter 158..700
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 187..398
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 411..679
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 505..510
/label=SDS
regulatory 707..716
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
regulatory 711..720
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 713..2804
/codon_start=1
/gene="NEO1"
/gene_synonym="IGDCC2; NGN; NTN1R2"
/product="neogenin isoform 1 precursor"
/label=NEO1
/note="isoform 1 precursor is encoded by transcriptvariant
1; immunoglobulin superfamily DCC subclass member 2;
neogenin homolog 1"
/db_xref="GI:157311649"
/db_xref="CCDS:CCDS10247.1"
/db_xref="GeneID:4756"
/db_xref="HGNC:HGNC:7754"
/db_xref="MIM:601907"
/protein_id="NP_002490.2"
/translation="MAAERGARRLLSTPSFWLYCLLLLGRRAPGAAAARSGSAPQSPGA
SIRTFTPFYFLVEPVDTLSVRGSSVILNCSAYSEPSPKIEWKKDGTFLNLVSDDRRQLL
PDGSLFISNVVHSKHNKPDEGYYQCVATVESLGTIISRTAKLIVAGLPRFTSQPEPSSV
YAGNNAILNCEVNADLVPFVRWEQNRQPLLLDDRVIKLPSGMLVISNATEGDGGLYRCV
VESGGPPKYSDEVELKVLPDPEVISDLVFLKQPSPLVRVIGQDVVLPCVASGLPTPTIK
WMKNEEALDTESSERLVLLAGGSLEISDVTEDDAGTYFCIADNGNETIEAQAELTVQAQ
PEFLKQPTNIYAHESMDIVFECEVTGKPTPTVKWVKNGDMVIPSDYFKIVKEHNLQVLG
LVKSDEGFYQCIAENDVGNAQAGAQLIILEHAPATTGPLPSAPRDVVASLVSTRFIKLT
WRTPASDPHGDNLTYSVFYTKEGIARERVENTSHPGEMQVTIQNLMPATVYIFRVMAQN
KHGSGESSAPLRVETQPEVQLPGPAPNLRAYAASPTSITVTWETPVSGNGEIQNYKLYY
MEKGTDKEQDVDVSSHSYTINGLKKYTEYSFRVVAYNKHGPGVSTPDVAVRTLSDVPSA
APQNLSLEVRNSKSIMIHWQPPAPATQNGQITGYKIRYRKASRKSDVTETLVSGTQLSQ
LIE"
misc_feature 753..758
/label=SDS
misc_feature 1569..1574
/label=SDS
intron 2805..2845
/label=Splicing Donor
misc_feature 2849..2882
/label=lox2272-JT1571
repeat_region 2927..3067
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 2927..3056
/label=AAV2 ITR
repeat_region 2928..3068
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
rep_origin 3142..3597
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 3879..3983
/gene="bla"
/label=AmpR promoter
CDS 3984..4844
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5015..5603
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtcgac tctagaggat ctgcgatcgc tccggtgccc
181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
241 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
361 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
421 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
481 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
541 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
601 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
661 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac cgtacggcca ccatggcggc
721 ggagcgggga gcccggcgac tcctcagcac cccctccttc tggctctact gcctgctgct
781 gctcgggcgc cgggcgccgg gcgccgcggc cgccaggagc ggctccgcgc cgcagtcccc
841 aggagccagc attcgaacgt tcactccatt ttattttctg gtggagccgg tggatacact
901 ctcagttaga ggctcttctg ttatattaaa ctgttcagca tattctgagc cttctccaaa
961 aattgaatgg aaaaaagatg gaactttttt aaacttagta tcagatgatc gacgccagct
1021 tctcccggat ggatctttat ttatcagcaa tgtggtgcat tccaaacaca ataaacctga
1081 tgaaggttat tatcagtgtg tggccactgt tgagagtctt ggaactatta tcagtagaac
1141 agcgaagctc atagtagcag gtcttccaag atttaccagc caaccagaac cttcctcagt
1201 ttatgctggg aacaatgcaa ttctgaattg tgaagttaat gcagatttgg tcccatttgt
1261 gaggtgggaa cagaacagac aaccccttct tctggatgat agagttatca aacttccaag
1321 tggaatgctg gttatcagca atgcaactga aggagatggc gggctttatc gctgcgtagt
1381 ggaaagtggt gggccaccaa agtatagtga tgaagttgaa ttgaaggttc ttccagatcc
1441 tgaggtgata tcagacttgg tatttttgaa acagccttct cccttagtca gagtcattgg
1501 tcaggatgta gtgttgccat gtgttgcttc aggacttcct actccaacca ttaaatggat
1561 gaaaaatgag gaggcacttg acacagaaag ctctgaaaga ttggtattgc tggcaggtgg
1621 tagcctggag atcagtgatg ttactgagga tgatgctggg acttattttt gtatagctga
1681 taatggaaat gagacaattg aagctcaagc agagcttaca gtgcaagctc aacctgaatt
1741 cctgaagcag cctactaata tatatgctca cgaatctatg gatattgtat ttgaatgtga
1801 agtgactgga aaaccaactc caactgtgaa gtgggtcaaa aatggggata tggttatccc
1861 aagtgattat tttaagattg taaaggaaca taatcttcaa gttttgggtc tggtgaaatc
1921 agatgaaggg ttctatcagt gcattgctga aaatgatgtt ggaaatgcac aagctggagc
1981 ccaactgata atccttgaac atgcaccagc cacaacggga ccactgcctt cagctcctcg
2041 ggatgtcgtg gcctccctgg tctctacccg cttcatcaaa ttgacgtggc ggacacctgc
2101 atcagatcct cacggagaca accttaccta ctctgtgttc tacaccaagg aagggattgc
2161 tagggaacgt gttgagaata ccagtcaccc aggagagatg caagtaacca ttcaaaacct
2221 aatgccagcg accgtgtaca tctttagagt tatggctcaa aataagcatg gctcaggaga
2281 gagttcagct ccactgcgag tagaaacaca acctgaggtt cagctccctg gcccagcacc
2341 taaccttcgt gcatatgcag cttcgcctac ctccatcact gttacgtggg aaacaccagt
2401 gtctggcaat ggggaaattc agaattataa gttgtactac atggaaaagg ggactgataa
2461 agaacaggat gttgatgttt caagtcactc ttacaccatt aatgggttga aaaaatatac
2521 agagtatagt ttccgagtgg tggcctacaa taaacatggt cctggagttt ccacaccaga
2581 tgttgctgtt cgaacattgt cagatgttcc cagtgctgct cctcagaatc tgtccttgga
2641 agtgagaaat tcaaagagta ttatgattca ctggcagcca cctgctccag ccacacaaaa
2701 tgggcagatt actggctaca agattcgcta ccgaaaggcc tcccgaaaga gtgatgtcac
2761 tgagaccttg gtaagcggga cacagctgtc tcagctgatt gaaggtaagt atcaaggtta
2821 caagacaggt ttaaggagac caataagcta ttattcgtat aggatacttt atacgaagtt
2881 attaagttat ggagaccggt gtggcacgtg cggaccgagc ggccgcagga acccctagtg
2941 atggagttgg ccactccctc tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag
3001 gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc gcgcagctgc
3061 ctgcaggggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc
3121 atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
3181 tggttacgcg cagcgtgacc gctacacttg ccagcgcctt agcgcccgct cctttcgctt
3241 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
3301 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgatttgg
3361 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
3421 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aactctatct
3481 cgggctattc ttttgattta taagggattt tgccgatttc ggtctattgg ttaaaaaatg
3541 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaattttat
3601 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc
3661 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
3721 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
3781 cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
3841 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
3901 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
3961 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
4021 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
4081 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
4141 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
4201 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
4261 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
4321 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
4381 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
4441 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
4501 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
4561 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
4621 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
4681 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
4741 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
4801 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
4861 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
4921 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
4981 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
5041 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
5101 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
5161 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
5221 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
5281 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
5341 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
5401 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
5461 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
5521 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
5581 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
5641 tttgctggcc ttttgctcac atgt
//
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