| 质粒名称: | pYK250 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000808 |
| 产品用途: | Mammalian PhCMV-driven constitutive NLS-TetR-3xANR expression vector (PhCMV-NLS-TetR-3xANR-pA). |
| 存储实验室: | Anping ZENG Lab |
|
载体骨架基本信息 |
|
| 载体名称: |
pCMV
|
| 表达系统: | 哺乳动物表达 |
| 启动子: | CMV |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | NLS-TetR-3xANR |
| 物种来源: |
synthetic
|
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Programmable solid-state condensates for spatiotemporal control of mammalian gene expression
Wang Y., Jiang J., Xiong Q., Li S., Shao J., Xie M., Zeng A.P.
Nat. Chem. Biol. 2025 1–10 (2025)
|
pYK250
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pYK250载体质粒基本信息
pYK250质粒图谱载体图谱和pYK250载体序列质粒序列多克隆位点信息
pYK250质粒载体简介
pYK250质粒序列载体序列
LOCUS pYK250 6532 bp DNA circular SYN 11-FEB-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6532)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jul 27, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6532
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 232..819
/label=CMV
primer_bind 769..789
/label=CMV-F
primer_bind 863..882
/label=T7
primer_bind 881..993
/label=oYK248_NLStetR_F
primer_bind 881..900
/label=oYK232_tTA-F
primer_bind 881..900
/label=oYK06-ANR-mEGFP-F
CDS 905..964
/codon_start=1
/label=NLS
/translation="MPKKKRKVEASASPKKKRKV"
primer_bind 907..925
/label=oYK648_NLS_F
primer_bind 932..999
/label=oYK232_tTA-F
CDS 965..1583
/label=Translation 2573-5638
misc_feature 965..1582
/label=tetR
primer_bind complement(1564..1582)
/label=oYK278_tetR_R
primer_bind complement(1565..1602)
/label=oYK248_TetR-R
primer_bind 1582..1602
/label=oLZ048 NSP3-F
misc_feature 1583..1618
/label=linker
primer_bind 1583..1612
/label=oSCL388
misc_feature 1583..1603
/label=linker
primer_bind 1583..1602
/label=oSCL1192
primer_bind 1583..1602
/label=oSCL1129
primer_bind 1583..1600
/label=oLZ147 NS3a F
primer_bind 1583..1599
/label=oYF0033
primer_bind 1583..1598
/label=oYK28-mCherry-NS3a-F
primer_bind complement(1593..1618)
/label=oSCL1119
primer_bind complement(1593..1612)
/label=oSCL075
primer_bind complement(1597..1632)
/label=oYK92_L7Ae_R
primer_bind complement(1601..1619)
/label=oYK75_NLS_mcp_R
primer_bind complement(1607..1661)
/label=oSCL388
CDS 1613..1687
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
primer_bind complement(1613..1658)
/label=oYK215_Mcp_B_R
primer_bind complement(1613..1632)
/label=oYK195_ANR_R
primer_bind 1613..1627
/label=oYK82_LinkANR_F
primer_bind complement(1614..1633)
/label=test2
CDS 1624..1631
/codon_start=1
/label=NS3a H1
/translation="GS"
primer_bind 1639..1658
/label=oYK215_ANR1rep_F
primer_bind complement(1639..1658)
/label=oYK215_ANR1rep_R
primer_bind 1668..1687
/label=test1
primer_bind complement(1671..1687)
/label=TEST3
CDS 1688..1750
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
primer_bind 1688..1703
/label=Primer 4
primer_bind complement(1727..1770)
/label=oYK195_ANR_R
primer_bind complement(1732..1750)
/label=oYK82_LinkANR_R
primer_bind 1735..1749
/label=Primer 3
primer_bind complement(1746..1796)
/label=oYK215_Mcp_B_R
CDS 1751..1825
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
primer_bind complement(1751..1770)
/label=oYK92_L7Ae_R
primer_bind 1751..1765
/label=oYK82_LinkANR_F
primer_bind complement(1752..1771)
/label=test2
primer_bind 1777..1796
/label=oYK215_ANR1rep_F
primer_bind complement(1777..1796)
/label=oYK215_ANR1rep_R
primer_bind 1806..1825
/label=test1
primer_bind complement(1809..1825)
/label=TEST3
CDS 1826..1888
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
primer_bind 1826..1841
/label=Primer 4
primer_bind complement(1865..1908)
/label=oYK195_ANR_R
primer_bind complement(1870..1888)
/label=oYK82_LinkANR_R
primer_bind 1873..1887
/label=Primer 3
primer_bind complement(1884..1934)
/label=oYK215_Mcp_B_R
CDS 1889..1963
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
primer_bind complement(1889..1908)
/label=oYK92_L7Ae_R
primer_bind 1889..1903
/label=oYK82_LinkANR_F
primer_bind complement(1890..1909)
/label=test2
primer_bind 1915..1934
/label=oYK215_ANR1rep_F
primer_bind complement(1915..1934)
/label=oYK215_ANR1rep_R
primer_bind 1944..1963
/label=test1
primer_bind complement(1947..1963)
/label=TEST3
CDS 1964..2026
/codon_start=1
/label=ANR
/translation="GELDELVYLLDGPGYDPIHSD"
primer_bind 1964..1979
/label=Primer 4
primer_bind complement(2003..2026)
/label=oYK195_ANR_R
primer_bind complement(2008..2049)
/label=oYK82_LinkANR_R
primer_bind 2011..2025
/label=Primer 3
primer_bind complement(2027..2053)
/label=oYK278_tetR_R
primer_bind complement(2030..2052)
/label=oYK57-MCP-mCherry-R
terminator 2126..2353
/label=bGH PA
misc_feature 2399..2827
/label=f1 origin
misc_feature 2832..3175
/label=SV40 early promoter and origin
misc_feature 3021..3098
/label=SV40 ORI
CDS 3240..4031
/label=Kan/neoR
misc_feature 4205..4335
/label=SV40 early polyadenylation signal
primer_bind complement(4371..4391)
/label=M13-rev
misc_binding complement(4397..4419)
/label=LacO
promoter complement(4424..4453)
/label=lac
misc_feature complement(4718..5391)
/label=pUC origin
CDS complement(5539..6198)
/label=AmpR
promoter complement(6438..6466)
/label=Amp prom
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 caccatgccc aagaagaagc gcaaggtgga agctagcgct tccccgaaga aaaagcggaa
961 agtcatgtct agactggaca agagcaaagt cataaactct gctctggaat tactcaatga
1021 agtcggtatc gaaggcctga cgacaaggaa actcgctcaa aagctgggag ttgagcagcc
1081 taccctgtac tggcacgtga agaacaagcg ggccctgctc gatgccctgg caatcgagat
1141 gctggacagg catcataccc acttctgccc cctggaaggc gagtcatggc aagactttct
1201 gcggaacaac gccaagtcat tccgctgtgc tctcctctca catcgcgacg gggctaaagt
1261 gcatctcggc acccgcccaa cagagaaaca gtacgaaacc ctggaaaatc agctcgcgtt
1321 cctgtgtcag caaggcttct ccctggagaa cgcactgtac gctctgtccg ccgtgggcca
1381 ctttacactg ggctgcgtat tggaggatca ggagcatcaa gtagcaaaag aggaaagaga
1441 gacacctacc accgattcta tgcccccact tctgagacaa gcaattgagc tgttcgacca
1501 tcagggagcc gaacctgcct tccttttcgg cctggaacta atcatatgtg gcctggagaa
1561 acagctaaag tgcgaaagcg gcgccagcgg aagtggcgga ggaggcgacg tcggctccgg
1621 aacgggcagc gggaccggaa gtggcaccgg aaccacttcc ggaaccggta ctggcgggag
1681 taccggcggt gagctagacg aactcgtgta cctgttggat ggccccggtt acgacccaat
1741 ccacagcgat ggctccggaa cgggcagcgg gaccggaagt ggcaccggaa ccacttccgg
1801 aaccggtact ggcgggagta ccggcggtga gctagacgaa ctcgtgtacc tgttggatgg
1861 ccccggttac gacccaatcc acagcgatgg ctccggaacg ggcagcggga ccggaagtgg
1921 caccggaacc acttccggaa ccggtactgg cgggagtacc ggcggtgagc tagacgaact
1981 cgtgtacctg ttggatggcc ccggttacga cccaatccac agcgattaag gatccactag
2041 tccagtgtgg tggaattctg cagatatcca gcacagtggc ggccgctcga gtctagaggg
2101 cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
2161 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
2221 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
2281 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
2341 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac
2401 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
2461 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
2521 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
2581 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
2641 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
2701 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
2761 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
2821 gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
2881 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
2941 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
3001 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
3061 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc
3121 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg
3181 gagcttgtat atccattttc ggatctgatc aagagacagg atgaggatcg tttcgcatga
3241 ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct
3301 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc
3361 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg
3421 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg
3481 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc
3541 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc
3601 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg
3661 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc
3721 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg
3781 aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc
3841 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag
3901 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg
3961 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg
4021 agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc
4081 atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
4141 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
4201 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
4261 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
4321 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata
4381 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
4441 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
4501 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
4561 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
4621 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
4681 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
4741 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
4801 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
4861 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
4921 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
4981 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
5041 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
5101 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
5161 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
5221 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
5281 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
5341 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
5401 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
5461 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
5521 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
5581 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
5641 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
5701 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
5761 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
5821 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
5881 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
5941 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
6001 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
6061 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
6121 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
6181 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
6241 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
6301 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
6361 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
6421 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
6481 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tc
//
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