| 质粒名称: | pPD49_83 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 1448 |
| 存储实验室: | Andrew Fire |
|
载体骨架基本信息 |
|
| 载体名称: | -- |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 3826 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 蠕虫表达式 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | -- |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | hsp16-25', hsp16-415', IVSA, rf2128 |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pPD49_83 was a gift from Andrew Fire (Addgene plasmid # 1448 ; http://n2t.net/addgene:1448 ; RRID:Addgene_1448) |
买家导航
pPD49_83载体 pPD4983质粒基本信息
pPD49_83质粒图谱 pPD4983载体图谱和pPD49_83载体序列 pPD4983质粒序列多克隆位点信息
pPD49_83质粒 pPD4983载体简介
pPD49_83质粒序列 pPD4983载体序列
LOCUS pPD49_83 3818 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pPD49_83
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3818)
AUTHORS Fire A et al
TITLE Fire Lab C. Elegans Vector Kit 1995
REFERENCE 2 (bases 1 to 3818)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..3818
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(1392..1414)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 1514..1533
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind complement(1611..1629)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 1697..1801
/gene="bla"
/label=AmpR promoter
CDS 1802..2662
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(2020..2039)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 2833..3421
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 3322..3341
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 3575..3592
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 3709..3730
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 3745..3775
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 3783..3799
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 3788..3810
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 3807..5
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 3807..5
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
ORIGIN
1 atgaccatga ttacgccaag cttgcatgcc tgcaggtcga ctctagagga tcaccaaaaa
61 cggaacgttg agctggacgg aaatagtggt aaagtgacat gattatagtt tgaagatttc
121 taatttcaca attagagcaa atgttgttcg gtatttattt tcaacggtat ttatactatt
181 ttccaccttt ttctagaaca ttcgagctgc ttgttgcaaa aggagggcga ctcacattcg
241 gtacatggaa aagtagtgta cacaataaag agacccagat acattttccg tctgcgtctc
301 tttgcaccca ccgggagtat tttcaaacga atgcatctag gaccttctag aacattctgt
361 aaggctgcag aatgcgggta tataaggaaa gcgggctcag aggaagccaa cacgctttgt
421 tctagtgcat ctaaaaaact tcgaaaatcc tcatcggatc cccgggattg gccaaaggac
481 ccaaaggtat gtttcgaatg atactaacat aacatagaac attttcagga ggacccttgg
541 ctagcgtcga cggtaccatg gtattgatat ctgagctccg catcggccgc tgtcatcaga
601 tcgccatctc gcgcccgtgc ctctgacttc taagtccaat tactcttcaa catccctaca
661 tgctctttct ccctgtgctc ccacccccta tttttgttat tatcaaaaaa cttctcttaa
721 tttctttgtt ttttagcttc ttttaagtca cctctaacaa tgaaattgtg tagattcaaa
781 aatagaatta attcgtaata aaaagtcgaa aaaaattgtg ctccctcccc ccattaataa
841 taattctatc ccaaaatcta cacaatgttc tgtgtacact tcttatgttt tttacttctg
901 ataaattttt ttgaaacatc atagaaaaaa ccgcacacaa aataccttat catatgttac
961 gtttcagttt atgaccgcaa tttttatttc ttcgcacgtc tgggcctctc atgacgtcaa
1021 atcatgctca tcgtgaaaaa gttttggagt atttttggaa tttttcaatc aagtgaaagt
1081 ttatgaaatt aattttcctg cttttgcttt ttggggtttc ccctattgtt tgtcaagatt
1141 tcgaggacgg cgtttttctt gctaaaatca caagtattga tgagcacgat gcaagaaaga
1201 tcggaagaag gtttgggttt gaggctcagt ggaaggtgag tagaagttga taatttgaaa
1261 gtggagtagt gtctatgggg tttttgcctt aaatgacaga atacattccc aatataccaa
1321 acataactgt ttcctactag tcggccgtac gggccctttc gtctcgcgcg tttcggtgat
1381 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg
1441 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc
1501 tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa
1561 ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc ggccttaagg gcctcgtgat
1621 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
1681 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
1741 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
1801 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
1861 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
1921 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
1981 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
2041 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
2101 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
2161 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
2221 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
2281 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
2341 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
2401 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
2461 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
2521 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
2581 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
2641 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
2701 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
2761 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
2821 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
2881 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
2941 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt
3001 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
3061 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
3121 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
3181 ggagcgaacg acctacaccg aactgagata cctacagcgt gagcattgag aaagcgccac
3241 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
3301 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
3361 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
3421 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
3481 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
3541 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
3601 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
3661 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
3721 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
3781 aattgtgagc ggataacaat ttcacacagg aaacagct
//
