| 质粒名称: | pLU-CMV-Vimentin-NeonOxIrr_C199S |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000165 |
| 产品用途: | Expresses the Vimentin-NeonOxIrr/C199S fusion of the mutant of the irreversible green indicator for hydrogen peroxide with blocked sensitivity to hydrogen peroxide with vimentin in mammalian cells |
| 存储实验室: | Laboratory of Molecular Design |
|
载体骨架基本信息 |
|
| 载体名称: |
pLU
|
| 表达系统: | 慢病毒，哺乳动物表达 |
| 启动子: | CMV |
| 拷贝数: | 低拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Mach1 |
|
插入基因信息 |
|
| 插入基因名称: | Vimentin-NeonOxIrr/C199S |
| 物种来源: | Synthetic |
| 密码子优化: | 细菌表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Slowly Reducible Genetically Encoded Green Fluorescent Indicator for In Vivo and Ex Vivo Visualization of Hydrogen Peroxide
Subach O.M., Kunitsyna T.A., Mineyeva O., Lazutkin A.A., Bezryadnov D., Barykina N.V., Piatkevich K.D., Ermakova Y., Bilan D., Belousov V., Anokhin K.V., Enikolopov G.N., Subach F.V.
Int. J. Mol. Sci. 2019, Vol. 20, Page 3138 20, 3138 (2019)
|
买家导航
pLU-CMV-Vimentin-NeonOxIrr_C199S载体质粒基本信息
pLU-CMV-Vimentin-NeonOxIrr_C199S质粒图谱载体图谱和pLU-CMV-Vimentin-NeonOxIrr_C199S载体序列质粒序列多克隆位点信息
pLU-CMV-Vimentin-NeonOxIrr_C199S质粒载体简介
pLU-CMV-Vimentin-NeonOxIrr_C199S质粒序列载体序列
LOCUS pLU-CMV-Vimentin-NeonOxIrr_C199S 9339 bp DNA circular SYN 22-APR-2023
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9339)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 23, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..9339
/mol_type="other DNA"
/organism="synthetic DNA construct"
LTR 234..414
/label=5' LTR
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 458..583
/label=HIV-1 psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1076..1309
/label=HIV-1 RRE
/note="The Rev response element (RRE) of human
immunodeficiency virus type 1 allows for Rev-dependent mRNA
export from the nucleus to the cytoplasm."
misc_feature 1798..1915
/label=HIV-1 cPPT
/note="central polypurine tract from pol gene of human
immunodeficiency virus type 1"
enhancer 1936..2241
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 2242..2445
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
CDS 2527..3924
/codon_start=1
/label=Vimentin
/translation="MSTRSVSSSSYRRMFGGPGTASRPSSSRSYVTTSTRTYSLGSALR
PSTSRSLYASSPGGVYATRSSAVRLRSSVPGVRLLQDSVDFSLADAINTEFKNTRTNEK
VELQELNDRFANYIDKVRFLEQQNKILLAELEQLKGQGKSRLGDLYEEEMRELRRQVDQ
LTNDKARVEVERDNLAEDIMRLREKLQEEMLQREEAENTLQSFRQDVDNASLARLDLER
KVESLQEEIAFLKKLHEEEIQELQAQIQEQHVQIDVDVSKPDLTAALRDVRQQYESVAA
KNLQEAEEWYKSKFADLSEAANRNNDALRQAKQESTEYRRQVQSLTCEVDALKGTNESL
ERQMREMEENFAVEAANYQDTIGRLQDEIQNMKEEMARHLREYQDLLNVKMALDIEIAT
YRKLLEGEESRISLPLPNFSSLNLRETNLDSLPLVDTHSKRTLLIKTVETRDGQVINET
SQHHDDLE"
CDS 3946..5379
/codon_start=1
/label=NeonOxIrr/C199S
/translation="MEMASQQGETMSGPLHIGLISTVGPYLLPHIIPMLHQTFPKLEMY
LHEAQTHQLLAQLDSGKLDCVILAPVKESEAFIEVPLFDEPMLLAIYEDHPWANRECVP
MADLAGEKLLMLEDGHSLRDQAMAYFADWCRSKKTYPNDKTIISTFKWSYTTGNGKRYR
STARTTYTFAKPMAANYLKNQPMYVVRKTELKHSKTELNFKEWQKAFTDVMGMDELYKV
DGGSGGTGMASKGEEDNMASLPATHELHIFGSINGADFDMVGQGTGNPIDGYEELNLKS
TKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFEDGASLTV
NYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAIDNGFCFEAGADEDTHFRATSLET
LRNMVAAGSGITLLPALAVPPERKRDGVVYLPCTKPEPRRTIGLVYRPGSPLRSRYEQL
AEAIRARMDGHFDKVLKQAV"
primer_bind 4275..4294
/label=WKG165-F
misc_feature 5426..6014
/label=WPRE
/note="woodchuck posttranscriptional regulatory element"
CDS complement(5897..5908)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
misc_feature 6101..6334
/label=3'LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
LTR 6154..6334
/label=5' LTR
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
polyA_signal 6406..6527
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin 6546..6681
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind complement(6714..6730)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 6738..6754
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(6762..6792)
/label=lac promoter
/note="promoter for the E. coli lac operon"
rep_origin complement(7116..7704)
/direction=LEFT
/label=ori
/note="high-copy-number colE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(7875..8735)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(8736..8840)
/gene="bla"
/label=AmpR promoter
primer_bind 9314..9330
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
ORIGIN
1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
901 taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg
961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
1921 taagcttggg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
1981 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
2041 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
2101 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
2161 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
2221 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
2281 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
2341 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
2401 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctcgttt agtgaaccgt
2461 cagatcgcct ggagacgcca tccacgctgt tttgacctcc atagaagaca ccgacggcgc
2521 gccaccatgt ccaccaggtc cgtgtcctcg tcctcctacc gcaggatgtt cggcggcccg
2581 ggcaccgcga gccggccgag ctccagccgg agctacgtga ctacgtccac ccgcacctac
2641 agcctgggca gcgcgctgcg ccccagcacc agccgcagcc tctacgcctc gtccccgggc
2701 ggcgtgtatg ccacgcgctc ctctgccgtg cgcctgcgga gcagcgtgcc cggggtgcgg
2761 ctcctgcagg actcggtgga cttctcgctg gccgacgcca tcaacaccga gttcaagaac
2821 acccgcacca acgagaaggt ggagctgcag gagctgaatg accgcttcgc caactacatc
2881 gacaaggtgc gcttcctgga gcagcagaat aagatcctgc tggccgagct cgagcagctc
2941 aagggccaag gcaagtcgcg cctgggggac ctctacgagg aggagatgcg ggagctgcgc
3001 cggcaggtgg accagctaac caacgacaaa gcccgcgtcg aggtggagcg cgacaacctg
3061 gccgaggaca tcatgcgcct ccgggagaaa ttgcaggagg agatgcttca gagagaggaa
3121 gccgaaaaca ccctgcaatc tttcagacag gatgttgaca atgcgtctct ggcacgtctt
3181 gaccttgaac gcaaagtgga atctttgcaa gaagagattg cctttttgaa gaaactccac
3241 gaagaggaaa tccaggagct gcaggctcag attcaggaac agcatgtcca aatcgatgtg
3301 gatgtttcca agcctgacct cacggctgcc ctgcgtgacg tacgtcagca atatgaaagt
3361 gtggctgcca agaacctgca ggaggcagaa gaatggtaca aatccaagtt tgctgacctc
3421 tctgaggctg ccaaccggaa caatgacgcc ctgcgccagg caaagcagga gtccactgag
3481 taccggagac aggtgcagtc cctcacctgt gaagtggatg cccttaaagg aaccaatgag
3541 tccctggaac gccagatgcg tgaaatggaa gagaactttg ccgttgaagc tgctaactac
3601 caagacacta ttggccgcct gcaggatgag attcagaata tgaaggagga aatggctcgt
3661 caccttcgtg aataccaaga cctgctcaat gttaagatgg cccttgacat tgagattgcc
3721 acctacagga agctgctgga aggcgaggag agcaggattt ctctgcctct tccaaacttt
3781 tcctccctga acctgaggga aactaatctg gattcactcc ctctggttga tacccactca
3841 aaaaggacac ttctgattaa gacggttgaa actagagatg gacaggttat caacgaaact
3901 tctcagcatc acgatgacct tgaaggggat ccagctagcc tcgagatgga gatggcaagc
3961 cagcagggcg agacgatgtc cggaccgctg cacattggtt tgatttccac agttggaccg
4021 tacctgctac cgcatattat ccctatgctg caccagacct ttccaaagct ggaaatgtat
4081 ctgcatgaag cgcagaccca ccagttactg gcgcaactgg acagcggcaa actcgattgc
4141 gtgatcctcg cgccggtgaa agagagcgaa gcattcattg aagtgccgtt gtttgatgag
4201 ccaatgttgc tggctatcta tgaagatcac ccgtgggcga accgcgagtg cgtaccgatg
4261 gccgatctgg caggggaaaa actgctgatg ctggaagatg gccactcttt gcgcgatcag
4321 gcaatggcgt acttcgctga ctggtgtaga tccaagaaga cttaccccaa tgataagact
4381 atcatttcca catttaagtg gtcttatacc accggcaacg gcaaacgcta ccggtctacc
4441 gctcgcacca catacacatt cgccaaacca atggccgcaa attacctcaa aaatcagccg
4501 atgtatgtcg ttcggaaaac agagctgaaa cattctaaga ccgaactgaa ttttaaagag
4561 tggcaaaagg cctttacgga cgtgatgggc atggacgagc tgtataaggt ggatggcggt
4621 agcggtggca ccggcatggc ctcaaaggga gaggaggaca acatggctag tctgcctgct
4681 acccacgaac tgcatatttt tggctccata aatggcgccg atttcgacat ggtaggccaa
4741 ggcaccggta atccaatcga cggttatgag gagctgaacc tgaagtccac caagggcgat
4801 ctgcagttca gtccctggat cctggtgccg cacattgggt acgggtttca tcagtatctt
4861 ccctatccag acgggatgtc accatttcag gctgcaatgg tcgacgggtc cggatatcag
4921 gtgcaccgca ctatgcagtt tgaggacggt gcttcactga ccgtgaatta cagatacacc
4981 tacgaaggat cacatatcaa aggggaagct caggtgaagg gcactgggtt cccagctgac
5041 ggccctgtga tgacaaattc cctgacagcc atcgacaacg gtttctgttt tgaagccggg
5101 gcggatgaag atacacactt ccgcgcgact agcctggaaa ctctgcgcaa catggtagcg
5161 gcaggtagcg ggatcacttt actgccagcg ctggctgtgc cgccggagcg caaacgcgat
5221 ggggttgttt atctgccgtg cactaagccg gaaccacgcc gcactattgg cctggtttat
5281 cgtcctggct caccgctgcg cagccgctat gagcagctgg ctgaggccat ccgcgcaaga
5341 atggatggcc atttcgataa agttttaaaa caggcggttt aaaccggtat ccgtctagat
5401 gtacaagtaa agcggccgcg tcgacaatca acctctggat tacaaaattt gtgaaagatt
5461 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
5521 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
5581 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
5641 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
5701 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
5761 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
5821 gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
5881 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
5941 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
6001 ggccgcctcc ccgcctggaa ttcgagctcg gtacctttaa gaccaatgac ttacaaggca
6061 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc
6121 caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga
6181 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
6241 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
6301 agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt
6361 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca
6421 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
6481 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc
6541 tagctatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
6601 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
6661 gaggaggctt ttttggaggc ctaggctttt gcgggcccaa attcgtaatc atggtcatag
6721 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
6781 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
6841 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
6901 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
6961 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
7021 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
7081 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
7141 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
7201 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
7261 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
7321 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
7381 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
7441 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
7501 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
7561 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
7621 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
7681 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
7741 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
7801 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
7861 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
7921 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
7981 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
8041 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
8101 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
8161 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
8221 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
8281 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
8341 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
8401 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
8461 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
8521 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
8581 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
8641 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
8701 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
8761 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
8821 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
8881 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg
8941 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
9001 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc
9061 gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat
9121 atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg
9181 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
9241 cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc
9301 cagtcacgac gttgtaaaac gacggccagt gccaagctg
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