pMS2M-PH-gagpol-D64V

LOCUS       pMS2M-PH-gagpol-D64               10711 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Expression of bacteriophage-derived MS2 coat protein fused HIV-1 
            Lentiviral Gag-Pol at N terminal with a HIV-1 protease cleavage 
            signal between MS2 and Gag-Pol. .
ACCESSION   .
VERSION     .
KEYWORDS    pMS2M-PH-gagpol-D64V
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10711)
  AUTHORS   Ling S, Yang S, Hu X, Yin D, Dai Y, Qian X, Wang D, Pan X, Hong J, 
            Sun X, Yang H, Paludan SR, Cai Y
  TITLE     Lentiviral delivery of co-packaged Cas9 mRNA and a Vegfa-targeting 
            guide RNA prevents wet age-related macular degeneration in mice.
  JOURNAL   Nat Biomed Eng. 2021 Jan 4. pii: 10.1038/s41551-020-00656-y. doi: 
            10.1038/s41551-020-00656-y.
  PUBMED    33398131
REFERENCE   2  (bases 1 to 10711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10711
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      857..866
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             866..1252
                     /codon_start=1
                     /product="bacteriophage MS2 coat protein"
                     /label=MS2-N55K
                     /note="binds with high affinity to a specific stem-loop 
                     structure in the viral RNA (Lim et al., 1994)"
                     /translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
                     CSVRQSSAQKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
                     VKAMQGLLKDGNPIPSAIAANSGIY"
     misc_feature    1277..1342
                     /label=MCS
                     /note="multiple cloning site"
     promoter        complement(6265..6283)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6266..6283)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6303..6320)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6309..6533
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6579..7006
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6666..6685)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6875..6896
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7015..7035)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7020..7349
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7200..7335
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7262..7281
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7416..8210
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7470..7489)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8080..8099
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8384..8505
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8421..8440)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8475..8494
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8554..8570)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8554..8570)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8567..8589)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8578..8594
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8602..8632)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8647..8668
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8785..8802)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8956..9544)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9036..9055)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9715..10575)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10338..10357
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10576..10680)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc
      841 gctagcgcta ccggtcgcca ccatggcctc taattttact caatttgtgc ttgtcgataa
      901 tggggggacg ggagatgtga ccgttgcccc tagcaatttc gcaaatggcg ttgcagaatg
      961 gatctctagc aacagcagaa gccaagcgta caaagtaacg tgttccgttc gccaaagctc
     1021 cgcccaaaaa cggaagtata caataaaggt tgaggtgccg aaagtagcca ctcaaacagt
     1081 tggtggggta gaattgcccg tagcggcatg gcggtcatat ctcaatatgg aactcactat
     1141 cccaatcttc gccacgaata gcgattgtga gctgatagtt aaggctatgc aaggtcttct
     1201 caaagatgga aaccctattc catctgctat cgccgccaac agcgggatat acagccagaa
     1261 ctatccgatt gtgcagtccg gactcagatc tcgagctcaa gcttcgaatt ctgcagtcga
     1321 cggtaccgcg ggcccgggat ccatggactc gggccgggac ttcctgaccc tgcacggcct
     1381 acaggatgat gaggatctac aggcgctgct gaagggcagc cagctcctga aggtgaagtc
     1441 cagctcatgg aggagagagc gcttctacaa gttgcaggag gactgcaaga ccatctggca
     1501 ggagtcccgc aaggtcatgc ggaccccgga gtcccagctg ttctccatcg aggacattca
     1561 ggaggtgcga atggggcacc gcacggaggg tctggagaag ttcgcccgtg atgtgcccga
     1621 ggaccgctgc ttctccattg tcttcaagga ccagcgcaat acactagacc tcatcgcccc
     1681 atcgccagct gatgcccagc actgggtgct ggggctgcac aagatcatcc accactcagg
     1741 ctccatggac cagcgtcaga agctacagca ctggattcac tcctgcttgc gaaaagctga
     1801 caaaaacaag gacaacaaga tgagcttcaa ggagctgcag aacttcctga aggagctcaa
     1861 catccagcct cgagccgaat tcaccctggc cgccagggcc agcgtgctga gcggcggcga
     1921 gctggacagg tgggagaaga tcaggctgag gcccggcggc aagaagaagt ataagctgaa
     1981 gcacatcgtg tgggccagca gggagctgga gaggttcgcc gtgaaccccg gcctgctgga
     2041 gaccagcgag ggctgcaggc agatcctggg ccagctgcag cccagcctgc agaccggcag
     2101 cgaggagctg aggagcctgt acaacaccgt ggccaccctg tactgcgtgc accagaggat
     2161 cgagatcaag gacaccaagg aggccctgga caagatcgag gaggagcaga acaagtccaa
     2221 gaagaaggcc cagcaggccg ccgccgacac cggccacagc agccaggtga gccagaacta
     2281 ccccatcgtg cagaacatcc agggccagat ggtgcaccag gccatcagcc ccaggaccct
     2341 gaacgcctgg gtgaaggtgg tggaggagaa ggccttcagc cccgaggtga tccccatgtt
     2401 cagcgccctg agcgagggag ccacccccca ggacctgaac accatgctga acaccgtggg
     2461 cggccaccag gccgccatgc agatgctgaa ggagaccatc aacgaggagg ccgccgagtg
     2521 ggacagggtg caccccgtgc acgccggccc catcgccccc ggccagatga gggagccccg
     2581 cggcagcgac atcgccggca ccaccagcac cctgcaggag cagatcggct ggatgaccaa
     2641 caaccccccc atccccgtgg gcgaaatcta caagaggtgg atcatcctgg gcctgaacaa
     2701 gatcgtgagg atgtacagcc ccaccagcat cctggatatc aggcagggcc ccaaagagcc
     2761 cttcagggac tacgtggaca ggttctacaa gaccctgcgc gccgagcagg ccagccagga
     2821 ggtgaagaac tggatgaccg agaccctgct ggtgcagaac gccaaccccg actgcaagac
     2881 catcctgaag gccctgggac ccgccgccac cctggaggag atgatgaccg cctgccaggg
     2941 cgtgggcggc cccggccaca aggccagggt gctggccgag gccatgagcc aggtgaccaa
     3001 caccgccacc atcatgatgc agaggggcaa cttcaggaac cagaggaaga tggtgaagtg
     3061 cttcaactgc ggcaaggagg gccacaccgc caggaactgc cgcgccccca ggaagaaggg
     3121 ctgctggaag tgcggcaagg agggccacca gatgaaggac tgcaccgaga ggcaggctaa
     3181 ttttttaggg aagatctggc cttcctacaa gggaaggcca gggaattttc ttcagagcag
     3241 accagagcca acagccccac catttcttca gagcagacca gagccaacag ccccaccaga
     3301 agagagcttc aggtctgggg tagagacaac aactccccct cagaagcagg agccgataga
     3361 caaggaactg tatcctttaa cttccctcag atcactcttt ggcaacgacc cctcgtcaca
     3421 ataaagatcg gtggccagct gaaggaggcc ctgctggaca ccggcgccga cgacaccgtg
     3481 ctggaggaga tgagcctgcc cggcaggtgg aagcccaaga tgatcggcgg catcggcggc
     3541 ttcatcaagg tgaggcagta cgaccagatc ctgatcgaga tctgcggcca caaggccatc
     3601 ggcaccgtgc tggtgggacc tacacctgtg aacatcatcg gcaggaacct gctgacccag
     3661 atcggctgca ccctgaactt ccccatcagc cccatcgaga ccgtgcccgt gaagctgaag
     3721 cccggcatgg acggccctaa ggtgaagcag tggcccctga ccgaggagaa gatcaaggcc
     3781 ctggtggaga tctgcaccga gatggagaag gagggcaaga tcagcaagat cggccccgag
     3841 aacccctaca acacccccgt gttcgccatc aagaagaagg acagcaccaa gtggaggaag
     3901 ctggtggact tcagggagct gaacaagagg acccaggact tctgggaggt gcagctgggc
     3961 atcccccacc ccgccggcct gaagaagaag aagagcgtga ccgtgctgga cgtgggcgac
     4021 gcctacttca gcgtgcccct ggacgaggac ttcaggaagt ataccgcctt caccatcccc
     4081 agcatcaaca acgagacccc cggcatccgc taccagtaca acgtgctgcc ccagggctgg
     4141 aagggcagcc ccgccatctt ccagagcagc atgacaaaga tcctggagcc cttcaagaag
     4201 cagaaccccg acatcgtgat ctatcagtac atggacgacc tgtacgtggg cagcgacctg
     4261 gagatcggcc agcacaggac caagatcgag gagctgaggc agcacctgct gaggtggggc
     4321 ctgaccaccc ccgacaagaa gcaccagaag gagcccccat tcctgtggat gggctacgag
     4381 ctgcaccccg acaagtggac cgtgcagccc atcgtgctgc ccgagaagga cagctggacc
     4441 gtgaacgaca ttcagaagct ggtgggcaag ctgaactggg ccagccagat ctaccctggc
     4501 atcaaggtga ggcagctgtg caagctgctg aggggcacaa aggctctgac cgaggtgatc
     4561 cccctgaccg aggaggccga gctggagctg gccgagaaca gggagatcct gaaggagccc
     4621 gtgcacggcg tgtactacga ccccagcaag gacctgatcg ccgagatcca gaagcagggc
     4681 cagggccagt ggacctacca gatctaccag gagcccttca agaacctgaa gaccggcaag
     4741 tacgcccgca tgcgcggcgc ccacaccaac gacgtgaagc agctgaccga ggccgtgcag
     4801 aagatcacca ccgagagcat cgtgatctgg ggcaagactc ctaagttcaa gctgcccatc
     4861 cagaaggaga cctgggagac ctggtggacc gagtactggc aggccacctg gattcccgag
     4921 tgggagttcg tgaacacccc tcccctggtg aagctgtggt atcagctgga gaaggagccc
     4981 atcgtgggcg ccgagacctt ctacgtggac ggcgccgcca acagggagac caagctgggc
     5041 aaggccggct acgtgaccaa caagggccgc cagaaggtgg tgcccctgac caacaccacc
     5101 aaccagaaga ccgagctgca ggctatctac ctggccctgc aggactcagg cctggaggtg
     5161 aacatcgtga ccgacagcca gtacgccctg ggcatcatcc aggcccagcc cgacaagagc
     5221 gagagcgagc tggtgaacca gatcatcgag cagctgatca agaaggagaa ggtgtacctg
     5281 gcctgggtgc ccgcccacaa gggcatcggc ggcaacgagc aggtggacaa gctggtgagc
     5341 gccggcatca ggaagatcct gttcctggac ggcatcgaca aggcccagga cgagcacgag
     5401 aagtaccaca gcaactggag ggctatggct agcgacttca acctgcctcc cgtggtggct
     5461 aaggagatcg tggccagctg cgacaagtgc cagctgaagg gcgaggccat gcacggccag
     5521 gtggactgca gccccggcat ctggcagctg gtttgcaccc acctggaggg caaggtgatc
     5581 ctggtggccg tgcacgtggc ctccggctac atcgaggccg aggtgatccc cgccgagacc
     5641 ggccaggaga ccgcctactt cctgctgaag ctggccggcc gctggcccgt gaagaccatc
     5701 cacaccgaca acggcagcaa cttcaccagc gccaccgtga aggctgcctg ctggtgggcc
     5761 ggcatcaagc aggagttcgg catcccctac aacccccagt ctcagggcgt ggtggagagc
     5821 atgaacaagg agctgaagaa gatcatcggc caggtgaggg accaggccga gcacctgaag
     5881 accgccgtgc agatggccgt gttcatccac aacttcaaga ggaagggcgg catcggcggc
     5941 tacagcgccg gcgagaggat cgtggacatc atcgccaccg acatccagac caaggagctg
     6001 cagaagcaga tcaccaagat ccagaacttc agggtgtact acagggacag caggaaccct
     6061 ctgtggaagg gccccgccaa gctgctgtgg aagggcgagg gcgccgtggt gatccaggac
     6121 aacagcgaca tcaaggtggt gcccaggagg aaggccaaga tcatcaggga ctacggcaag
     6181 cagatggccg gcgacgactg cgtggcctcc aggcaggacg aggactgata aggatccctc
     6241 gagcatgcat ctagagggcc ctattctata gtgtcaccta aatgctagag ctcgctgatc
     6301 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
     6361 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
     6421 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
     6481 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga
     6541 ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt
     6601 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     6661 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     6721 agctctaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     6781 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     6841 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     6901 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     6961 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg
     7021 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     7081 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     7141 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     7201 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     7261 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag
     7321 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg
     7381 gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg
     7441 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
     7501 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
     7561 tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt
     7621 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
     7681 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
     7741 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
     7801 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
     7861 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
     7921 aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg
     7981 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
     8041 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
     8101 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
     8161 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct
     8221 ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac
     8281 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat
     8341 cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc
     8401 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     8461 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc
     8521 gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     8581 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     8641 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     8701 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     8761 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     8821 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     8881 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     8941 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     9001 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     9061 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     9121 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     9181 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     9241 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     9301 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     9361 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     9421 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     9481 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     9541 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     9601 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     9661 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     9721 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     9781 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     9841 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     9901 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     9961 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
    10021 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
    10081 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
    10141 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
    10201 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
    10261 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
    10321 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
    10381 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
    10441 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
    10501 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
    10561 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
    10621 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
    10681 cacatttccc cgaaaagtgc cacctgacgt c
//