| 质粒名称: | pAAVLINK-3'-NCOR1-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001718 |
| 产品用途: | 3' AAVLINK plasmid for NCOR1 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | Promoterless |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | NCOR1 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-3'-NCOR1-FLAG
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-3'-NCOR1-FLAG载体质粒基本信息
pAAVLINK-3'-NCOR1-FLAG质粒图谱载体图谱和pAAVLINK-3'-NCOR1-FLAG载体序列质粒序列多克隆位点信息
pAAVLINK-3'-NCOR1-FLAG质粒载体简介
pAAVLINK-3'-NCOR1-FLAG质粒序列载体序列
LOCUS pAAVLINK-3'-NCOR1-FLAG 7350 bp DNA circular SYN 20-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7350)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 4, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7350
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
protein_bind 145..178
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 179..270
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 271..4089
/codon_start=1
/label=NCOR1
/translation="GTPALPQTGIPTEALVKGSISRMPIEDSSPEKGREEAASKGHVIY
EGKSGHILSYDNIKNAREGTRSPRTAHEISLKRSYESVEGNIKQGMSMRESPVSAPLEG
LICRALPRGSPHSDLKERTVLSGSIMQGTPRATTESFEDGLKYPKQIKRESPPIRAFEG
AITKGKPYDGITTIKEMGRSIHEIPRQDILTQESRKTPEVVQSTRPIIEGSISQGTPIK
FDNNSGQSAIKHNVKSLITGPSKLSRGMPPLEIVPENIKVVERGKYEDVKAGETVRSRH
TSVVSSGPSVLRSTLHEAPKAQLSPGIYDDTSARRTPVSYQNTMSRGSPMMNRTSDVTI
SSNKSTNHERKSTLTPTQRESIPAKSPVPGVDPVVSHSPFDPHHRGSTAGEVYRSHLPT
HLDPAMPFHRALDPAAAAYLFQRQLSPTPGYPSQYQLYAMENTRQTILNDYITSQQMQV
NLRPDVARGLSPREQPLGLPYPATRGIIDLTNMPPTILVPHPGGTSTPPMDRITYIPGT
QITFPPRPYNSASMSPGHPTHLAAAASAEREREREREKERERERIAAASSDLYLRPGSE
QPGRPGSHGYVRSPSPSVRTQETMLQQRPSVFQGTNGTSVITPLDPTAQLRIMPLPAGG
PSISQGLPASRYNTAADALAALVDAAASAPQMDVSKTKESKHEAARLEENLRSRSAAVS
EQQQLEQKTLEVEKRSVQCLYTSSAFPSGKPQPHSSVVYSEAGKDKGPPPKSRYEEELR
TRGKTTITAANFIDVIITRQIASDKDARERGSQSSDSSSSLSSHRYETPSDAIEVISPA
SSPAPPQEKLQTYQPEVVKANQAENDPTRQYEGPLHHYRPQQESPSPQQQLPPSSQAEG
MGQVPRTHRLITLADHICQIITQDFARNQVSSQTPQQPPTSTFQNSPSALVSTPVRTKT
SNRYSPESQAQSVHHQRPGSRVSPENLVDKSRGSRPGKSPERSHVSSEPYEPISPPQVP
VVHEKQDSLLLLSQRGAEPAEQRNDARSPGSISYLPSFFTKLENTSPMVKSKKQEIFRK
LNSSGGGDSDMAAAQPGTEIFNLPAVTTSGSVSSRGHSFADPASNLGLEDIIRKALMGS
FDDKVEDHGVVMSQPMGVVPGTANTSVVTSGETRREEGDPSPHSGGVCKPKLISKSNSR
KSKSPIPGQGYLGTERPSSVSSVHSEGDYHRQTPGWAWEDRPSSTGSTQFPYNPLTMRM
LSSTPPTPIACAPSAVNQAAPHQQNRIWEREPAPLLSAQYETLSDSDD"
misc_feature 2494..2499
/label=SDS
misc_feature 2995..3000
/label=SDS
misc_feature 3723..3728
/label=SDS
CDS 4096..4161
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 4165..4596
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 4309..4314
/label=SDS
repeat_region 4613..4753
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4613..4742
/label=AAV2 ITR
rep_origin 4828..5283
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5565..5669
/gene="bla"
/label=AmpR promoter
CDS 5670..6530
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6701..7289
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtataact tcgtatagga tactttatag caatttatga
181 aactgggctt gtcgagacag agaagactct tgcgtttctg ataggcacct attggtctta
241 ctgacatcca ctttgccttt ctctccacag ggcaccccgg ctctgcccca gactggcata
301 ccaacagagg ctttggtgaa ggggtccatt tcgagaatgc ccattgaaga cagcagtcct
361 gagaaaggca gagaggaagc tgcatccaaa ggccatgtta tttatgaagg caaaagtgga
421 catatcttgt catatgataa tattaagaat gcccgagaag ggactaggag tccaagaaca
481 gctcatgaaa tcagtttaaa gagaagctat gaatcagtgg aaggaaatat aaagcaaggg
541 atgtcaatga gggagtctcc tgtatcagca ccgttagagg ggctgatatg ccgagcatta
601 cccaggggga gtcctcattc tgacctcaaa gaaaggactg tattgtctgg ctccataatg
661 caggggacac caagagcaac aactgaaagc tttgaagatg gccttaaata tcccaaacaa
721 attaaaaggg aaagtcctcc catacgagca tttgaaggtg ccattaccaa aggaaaacca
781 tatgatggca tcaccaccat caaagaaatg gggcgttcca ttcatgagat tccaaggcaa
841 gatattttaa ctcaggaaag tcggaaaact ccagaagtgg tccagagcac acggccgata
901 attgagggtt ccatttccca gggcacacca ataaagtttg acaacaactc aggtcaatct
961 gccatcaaac acaatgtcaa atccttaatc acggggccta gcaaactatc ccgtggaatg
1021 cctccgctgg aaattgtgcc agagaacata aaagtggtag aacggggaaa atatgaggat
1081 gtgaaagcag gcgagaccgt gcgttcccgg cacacgtcag tggtaagctc tggcccctcc
1141 gttcttaggt ccacactgca tgaagctccc aaagcacaac tgagccctgg gatttatgat
1201 gacaccagtg cacggaggac ccctgtgagt tatcaaaaca ccatgtccag aggctcaccc
1261 atgatgaaca gaacttctga tgttacaatt tcttctaaca agtctaccaa tcatgaaagg
1321 aaatcgacac tgacccctac ccagagggaa agtatcccag cgaagtctcc agtgcctggg
1381 gtggaccctg tcgtgagcca cagtccgttt gatccccatc acagaggcag cactgcaggc
1441 gaggtttatc ggagccacct gcccacgcac ttggatccag ccatgccttt tcacagggct
1501 ttggatcctg cagcggctgc ttacctgttt cagagacagc tttcaccaac tccaggttac
1561 ccaagtcagt atcagcttta cgcaatggag aacacaagac agacaatctt aaatgattac
1621 attacctcac aacagatgca agtgaacttg cgtccagatg tggccagagg actctcccca
1681 agagagcagc cactgggtct cccataccca gcaacgagag gaatcattga cctgaccaat
1741 atgcctccaa caattttagt gcctcatcca gggggaacaa gcactcctcc catggacaga
1801 atcacttata ttcctggtac acagattact ttccctccca ggccgtacaa ctctgcttcc
1861 atgtctccag gacacccaac acaccttgca gctgctgcaa gtgctgagag ggaacgggaa
1921 cgggagcggg agaaggagcg ggagcgggaa cggattgctg cagcttcctc cgacctctac
1981 ctgcggccag gctcagaaca gcctggccga cctggcagtc atggatatgt tcgctcccct
2041 tccccttcag taagaactca ggagaccatg ttgcaacaga gacccagtgt tttccaagga
2101 accaatggaa ccagtgtaat cacacctttg gatccaactg ctcagctacg aatcatgcca
2161 ctgcctgctg ggggcccttc aataagccaa ggcctgccag cctcccgtta caacactgct
2221 gcggatgccc tggctgctct tgtggatgct gcagcttctg caccccagat ggatgtgtcc
2281 aaaacaaaag agagtaagca tgaagctgcc aggttagaag aaaatttgag aagcaggtca
2341 gcagcagtta gtgaacagca gcagctagag cagaaaaccc tggaggtgga gaagagatct
2401 gttcagtgtt tatacacttc ttcagccttt ccaagtggca agccccagcc tcattcttca
2461 gtagtttatt ctgaggctgg gaaagataaa gggcctcctc caaaatccag atatgaggaa
2521 gagctaagga ccagagggaa gactaccatt actgcagcta acttcataga cgtgatcatc
2581 acccggcaaa ttgcctcgga caaggatgcg agggaacgtg gctctcaaag ttcagactct
2641 tctagtagct tatcttctca caggtatgaa acacctagcg atgctattga ggtgataagt
2701 cctgccagct cacctgcgcc accccaggag aaactgcaga cctatcagcc agaggttgtt
2761 aaggcaaatc aagcggaaaa tgatcctacc agacaatatg aaggaccatt acatcactat
2821 cgaccacagc aggaatcacc atctccccaa caacagctgc ccccttcttc acaggcagag
2881 ggaatggggc aagtgcccag gacccatcgg ctgatcacac ttgctgatca catctgtcaa
2941 attatcacac aagattttgc tagaaatcaa gtttcctcgc agactcccca gcagcctcct
3001 acttctacat tccagaactc accttctgct ttggtatcta cacctgtgag gactaaaaca
3061 tcaaaccgtt acagcccaga atcccaggct cagtctgtcc atcatcaaag accaggttca
3121 agggtctctc cagaaaatct tgtggacaaa tccaggggaa gtaggcctgg aaaatcccca
3181 gagaggagtc acgtctcttc ggagccctac gagcccatct ccccacccca ggttccggtt
3241 gtgcatgaga aacaggacag cttgctgctc ttgtctcaga ggggcgcaga gcctgcagag
3301 cagaggaatg atgcccgctc accagggagt ataagctact tgccttcatt cttcaccaag
3361 cttgaaaata catcacccat ggttaaatca aagaagcagg agatttttcg taagttgaac
3421 tcctctggtg gaggtgactc tgatatggca gctgctcagc caggaactga gatctttaat
3481 ctgccagcag ttactacgtc aggctcagtt agctctagag gccattcttt tgctgatcct
3541 gccagtaatc ttgggctgga agacattatc aggaaggctc tcatgggaag ctttgatgac
3601 aaagttgagg atcatggagt tgtcatgtcc cagcctatgg gagtagtgcc tggtactgcc
3661 aacacctcag ttgtgaccag tggtgagaca cgaagagagg aaggggaccc atcacctcat
3721 tcaggaggag tttgcaaacc aaagctgatc agcaagtcaa acagcaggaa atctaagtct
3781 cctatacctg ggcaaggcta cttaggaacg gaacggccct cttcagtctc ctctgtacat
3841 tcagaagggg attaccatag gcagacgcca gggtgggcct gggaagacag gccctcttca
3901 acaggctcaa ctcagtttcc ttataaccct ctgactatgc ggatgctcag cagtactcca
3961 ccaacaccga ttgcatgtgc tccctctgcg gtgaaccaag cagctcctca ccaacagaac
4021 aggatctggg agcgagagcc tgccccactg ctctcagcac agtacgagac cctgtcggat
4081 agtgatgact gtacagacta caaagaccat gacggtgatt ataaagatca tgacatcgac
4141 tacaaggatg acgatgacaa gtaactcgag ataatcaacc tctggattac aaaatttgtg
4201 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
4261 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
4321 aatcctggtt agttcttgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
4381 caggggctcg gctgttgggc actgacaatt ccgtggtgtt tatttgtgaa atttgtgatg
4441 ctattgcttt atttgtaacc atctagcttt atttgtgaaa tttgtgatgc tattgcttta
4501 tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg
4561 tttcaggttc agggggagat gtgggaggtt ttttaacgga ccgagcggcc gcaggaaccc
4621 ctagtgatgg agttggccac tccctctctg cgcgctcgct cgctcactga ggccgggcga
4681 ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc
4741 agctgcctgc aggggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
4801 caccgcatac gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg
4861 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccttagcg cccgctcctt
4921 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
4981 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
5041 atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
5101 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaact
5161 ctatctcggg ctattctttt gatttataag ggattttgcc gatttcggtc tattggttaa
5221 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa
5281 ttttatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac
5341 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
5401 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
5461 aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
5521 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
5581 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
5641 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
5701 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
5761 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
5821 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
5881 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
5941 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
6001 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
6061 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
6121 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
6181 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
6241 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
6301 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
6361 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
6421 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
6481 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
6541 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
6601 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
6661 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
6721 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
6781 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
6841 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
6901 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
6961 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
7021 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
7081 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
7141 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
7201 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
7261 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
7321 tggccttttg ctggcctttt gctcacatgt
//
