pCMV-ABE7.8

LOCUS       pCMV-ABE7.8                8727 bp ds-DNA     circular SYN 06-AUG-2018
DEFINITION  expresses ABE7.8 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    pCMV-ABE7.8
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8727)
  AUTHORS   Gaudelli NM, Komor AC, Rees HA, Packer MS, Badran AH, Bryson DI, Liu
            DR
  TITLE     Programmable base editing of A*T to G*C in genomic DNA without DNA 
            cleavage.
  JOURNAL   Nature. 2017 Nov 23;551(7681):464-471. doi: 10.1038/nature24644. 
            Epub 2017 Oct 25.
  PUBMED    29160308
REFERENCE   2  (bases 1 to 8727)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 6, 2018 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8727
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             683..4783
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             4796..4816
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4825..4842
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4865..4882)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4871..5095
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5166..5182)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5166..5182)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5179..5201)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5190..5206
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5214..5244)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5259..5280
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5397..5414)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5568..6156)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5648..5667)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(6327..7187)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6950..6969
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7188..7292)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7371..7390)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7562..7941
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7942..8145
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8096..8116
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     8187..8206
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8187..8205
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ttcttctggt ggttctagcg gcagcgagac tcccgggacc tcagagtccg ccacacccga
       61 aagttctggt ggttcttctg gtggttcttc cgaagtcgag ttttcccatg agtactggat
      121 gagacacgca ttgactctcg caaagagggc tctcgatgaa cgcgaggtgc ccgtgggggc
      181 agtactcgtg ctcaacaatc gcgtaatcgg cgaaggttgg aatagggcaa tcggactcca
      241 cgaccccact gcacatgcgg aaatcatggc ccttcgacag ggagggcttg tgatgcagaa
      301 ttatcgactt atcgatgcga cgctgtacgt cacgtttgaa ccttgcgtaa tgtgcgcggg
      361 agctatgatt cactcccgca ttggacgagt tgtattcggt gttcgcaacg ccaagacggg
      421 tgccgcaggt tcactgatgg acgtgctgca ttacccaggc atgaaccacc gggtagaaat
      481 cacagaaggc atattggcgg acgaatgtaa cgcgctgttg tgttactttt ttcgcatgcg
      541 gaggcaggtc tttaacgccc agaaaaaagc acaatcctct actgactctg gtggttcttc
      601 tggtggttct agcggcagcg agactcccgg gacctcagag tccgccacac ccgaaagttc
      661 tggtggttct tctggtggtt ctgataaaaa gtattctatt ggtttagcca tcggcactaa
      721 ttccgttgga tgggctgtca taaccgatga atacaaagta ccttcaaaga aatttaaggt
      781 gttggggaac acagaccgtc attcgattaa aaagaatctt atcggtgccc tcctattcga
      841 tagtggcgaa acggcagagg cgactcgcct gaaacgaacc gctcggagaa ggtatacacg
      901 tcgcaagaac cgaatatgtt acttacaaga aatttttagc aatgagatgg ccaaagttga
      961 cgattctttc tttcaccgtt tggaagagtc cttccttgtc gaagaggaca agaaacatga
     1021 acggcacccc atctttggaa acatagtaga tgaggtggca tatcatgaaa agtacccaac
     1081 gatttatcac ctcagaaaaa agctagttga ctcaactgat aaagcggacc tgaggttaat
     1141 ctacttggct cttgcccata tgataaagtt ccgtgggcac tttctcattg agggtgatct
     1201 aaatccggac aactcggatg tcgacaaact gttcatccag ttagtacaaa cctataatca
     1261 gttgtttgaa gagaacccta taaatgcaag tggcgtggat gcgaaggcta ttcttagcgc
     1321 ccgcctctct aaatcccgac ggctagaaaa cctgatcgca caattacccg gagagaagaa
     1381 aaatgggttg ttcggtaacc ttatagcgct ctcactaggc ctgacaccaa attttaagtc
     1441 gaacttcgac ttagctgaag atgccaaatt gcagcttagt aaggacacgt acgatgacga
     1501 tctcgacaat ctactggcac aaattggaga tcagtatgcg gacttatttt tggctgccaa
     1561 aaaccttagc gatgcaatcc tcctatctga catactgaga gttaatactg agattaccaa
     1621 ggcgccgtta tccgcttcaa tgatcaaaag gtacgatgaa catcaccaag acttgacact
     1681 tctcaaggcc ctagtccgtc agcaactgcc tgagaaatat aaggaaatat tctttgatca
     1741 gtcgaaaaac gggtacgcag gttatattga cggcggagcg agtcaagagg aattctacaa
     1801 gtttatcaaa cccatattag agaagatgga tgggacggaa gagttgcttg taaaactcaa
     1861 tcgcgaagat ctactgcgaa agcagcggac tttcgacaac ggtagcattc cacatcaaat
     1921 ccacttaggc gaattgcatg ctatacttag aaggcaggag gatttttatc cgttcctcaa
     1981 agacaatcgt gaaaagattg agaaaatcct aacctttcgc ataccttact atgtgggacc
     2041 cctggcccga gggaactctc ggttcgcatg gatgacaaga aagtccgaag aaacgattac
     2101 tccatggaat tttgaggaag ttgtcgataa aggtgcgtca gctcaatcgt tcatcgagag
     2161 gatgaccaac tttgacaaga atttaccgaa cgaaaaagta ttgcctaagc acagtttact
     2221 ttacgagtat ttcacagtgt acaatgaact cacgaaagtt aagtatgtca ctgagggcat
     2281 gcgtaaaccc gcctttctaa gcggagaaca gaagaaagca atagtagatc tgttattcaa
     2341 gaccaaccgc aaagtgacag ttaagcaatt gaaagaggac tactttaaga aaattgaatg
     2401 cttcgattct gtcgagatct ccggggtaga agatcgattt aatgcgtcac ttggtacgta
     2461 tcatgacctc ctaaagataa ttaaagataa ggacttcctg gataacgaag agaatgaaga
     2521 tatcttagaa gatatagtgt tgactcttac cctctttgaa gatcgggaaa tgattgagga
     2581 aagactaaaa acatacgctc acctgttcga cgataaggtt atgaaacagt taaagaggcg
     2641 tcgctatacg ggctggggac gattgtcgcg gaaacttatc aacgggataa gagacaagca
     2701 aagtggtaaa actattctcg attttctaaa gagcgacggc ttcgccaata ggaactttat
     2761 gcagctgatc catgatgact ctttaacctt caaagaggat atacaaaagg cacaggtttc
     2821 cggacaaggg gactcattgc acgaacatat tgcgaatctt gctggttcgc cagccatcaa
     2881 aaagggcata ctccagacag tcaaagtagt ggatgagcta gttaaggtca tgggacgtca
     2941 caaaccggaa aacattgtaa tcgagatggc acgcgaaaat caaacgactc agaaggggca
     3001 aaaaaacagt cgagagcgga tgaagagaat agaagagggt attaaagaac tgggcagcca
     3061 gatcttaaag gagcatcctg tggaaaatac ccaattgcag aacgagaaac tttacctcta
     3121 ttacctacaa aatggaaggg acatgtatgt tgatcaggaa ctggacataa accgtttatc
     3181 tgattacgac gtcgatcaca ttgtacccca atcctttttg aaggacgatt caatcgacaa
     3241 taaagtgctt acacgctcgg ataagaaccg agggaaaagt gacaatgttc caagcgagga
     3301 agtcgtaaag aaaatgaaga actattggcg gcagctccta aatgcgaaac tgataacgca
     3361 aagaaagttc gataacttaa ctaaagctga gaggggtggc ttgtctgaac ttgacaaggc
     3421 cggatttatt aaacgtcagc tcgtggaaac ccgccaaatc acaaagcatg ttgcacagat
     3481 actagattcc cgaatgaata cgaaatacga cgagaacgat aagctgattc gggaagtcaa
     3541 agtaatcact ttaaagtcaa aattggtgtc ggacttcaga aaggattttc aattctataa
     3601 agttagggag ataaataact accaccatgc gcacgacgct tatcttaatg ccgtcgtagg
     3661 gaccgcactc attaagaaat acccgaagct agaaagtgag tttgtgtatg gtgattacaa
     3721 agtttatgac gtccgtaaga tgatcgcgaa aagcgaacag gagataggca aggctacagc
     3781 caaatacttc ttttattcta acattatgaa tttctttaag acggaaatca ctctggcaaa
     3841 cggagagata cgcaaacgac ctttaattga aaccaatggg gagacaggtg aaatcgtatg
     3901 ggataagggc cgggacttcg cgacggtgag aaaagttttg tccatgcccc aagtcaacat
     3961 agtaaagaaa actgaggtgc agaccggagg gttttcaaag gaatcgattc ttccaaaaag
     4021 gaatagtgat aagctcatcg ctcgtaaaaa ggactgggac ccgaaaaagt acggtggctt
     4081 cgatagccct acagttgcct attctgtcct agtagtggca aaagttgaga agggaaaatc
     4141 caagaaactg aagtcagtca aagaattatt ggggataacg attatggagc gctcgtcttt
     4201 tgaaaagaac cccatcgact tccttgaggc gaaaggttac aaggaagtaa aaaaggatct
     4261 cataattaaa ctaccaaagt atagtctgtt tgagttagaa aatggccgaa aacggatgtt
     4321 ggctagcgcc ggagagcttc aaaaggggaa cgaactcgca ctaccgtcta aatacgtgaa
     4381 tttcctgtat ttagcgtccc attacgagaa gttgaaaggt tcacctgaag ataacgaaca
     4441 gaagcaactt tttgttgagc agcacaaaca ttatctcgac gaaatcatag agcaaatttc
     4501 ggaattcagt aagagagtca tcctagctga tgccaatctg gacaaagtat taagcgcata
     4561 caacaagcac agggataaac ccatacgtga gcaggcggaa aatattatcc atttgtttac
     4621 tcttaccaac ctcggcgctc cagccgcatt caagtatttt gacacaacga tagatcgcaa
     4681 acgatacact tctaccaagg aggtgctaga cgcgacactg attcaccaat ccatcacggg
     4741 attatatgaa actcggatag atttgtcaca gcttgggggt gactctggtg gttctcccaa
     4801 gaagaagagg aaagtctaac cggtcatcat caccatcacc attgagttta aacccgctga
     4861 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
     4921 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
     4981 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
     5041 ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct
     5101 gaggcggaaa gaaccagctg gggctcgata ccgtcgacct ctagctagag cttggcgtaa
     5161 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     5221 cgagccggaa gcataaagtg taaagcctag ggtgcctaat gagtgagcta actcacatta
     5281 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     5341 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     5401 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     5461 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     5521 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     5581 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     5641 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     5701 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     5761 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     5821 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     5881 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     5941 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     6001 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     6061 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     6121 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     6181 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     6241 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     6301 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     6361 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     6421 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     6481 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     6541 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     6601 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     6661 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     6721 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     6781 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     6841 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     6901 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     6961 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     7021 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     7081 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     7141 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     7201 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     7261 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     7321 gtcgacggat cgggagatcg atctcccgat cccctagggt cgactctcag tacaatctgc
     7381 tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag
     7441 tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag
     7501 aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag atatacgcgt
     7561 tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc
     7621 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
     7681 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
     7741 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
     7801 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
     7861 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
     7921 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
     7981 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
     8041 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
     8101 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt
     8161 cagatccgct agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat
     8221 gtccgaagtc gagttttccc atgagtactg gatgagacac gcattgactc tcgcaaagag
     8281 ggcttgggat gaacgcgagg tgcccgtggg ggcagtactc gtgcataaca atcgcgtaat
     8341 cggcgaaggt tggaataggc cgatcggacg ccacgacccc actgcacatg cggaaatcat
     8401 ggcccttcga cagggagggc ttgtgatgca gaattatcga cttatcgatg cgacgctgta
     8461 cgtcacgctt gaaccttgcg taatgtgcgc gggagctatg attcactccc gcattggacg
     8521 agttgtattc ggtgcccgcg acgccaagac gggtgccgca ggttcactga tggacgtgct
     8581 gcatcaccca ggcatgaacc accgggtaga aatcacagaa ggcatattgg cggacgaatg
     8641 tgcggcgctg ttgtccgact tttttcgcat gcggaggcag gagatcaagg cccagaaaaa
     8701 agcacaatcc tctactgact ctggtgg
//