Lenti-mEGFP-AT5G60980

LOCUS       Lenti-mEGFP-AT5G60980              10324 bp DNA     circular SYN 11-DEC-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10324)
  AUTHORS   Yoo AS, Sun AX, Li L, Shcheglovitov A, Portmann T, Li Y, Lee-Messer 
            C, Dolmetsch RE, Tsien RW, Crabtree GR
  TITLE     MicroRNA-mediated conversion of human fibroblasts to neurons.
  JOURNAL   Nature. 2011 Jul 13. doi: 10.1038/nature10323.
  PUBMED    21753754
REFERENCE   2  (bases 1 to 10324)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 1, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2011 Jul 13. doi: 10.1038/nature10323."
FEATURES             Location/Qualifiers
     source          1..10324
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     misc_feature    2202..2209
                     /label=enzyme cut site
     enhancer        2210..2513
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2514..2717
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      2756..2765
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2762..3478
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             3509..4888
                     /codon_start=1
                     /label=AT5G3BP1 (60980)
                     /translation="MAQQEASPSPGAEVVGRAFVEQYYHILHQSPGLVHRFYQDSSFLT
                     RPDVTGAVTTVTTMQAINDKILSLKYEDYTAEIETADAQESHERGVIVLVTGRLTGNDN
                     VRKKFSQSFFLAPQDKGYFVLNDVFRFLEEKEVTAQARSVPINGTTRDVQAPIEPERVV
                     VSHEPEVEPEPVASIEEEDLDNVAEVYDPSDKDEGVVVDVEPIEPPTQISHNEILSVPQ
                     GDAPKHSYASILKQMKSSPAPTTHVARNKPRPAPVNQKLTAPPAEPAARPEASAHENVP
                     NSSHVDVEDDGHSIYVRNLPFDSTPTQLEEVFKNFGAIKHEGIQVRSNKQQGFCFGFVE
                     FETSSGKQSALEASPVTIGDRQAVVEEKKTNSRGGGNNGGSRGRYFSGRGSFRNESFKG
                     GRGGGGRGGYGRGGGEFSGRPKSSNPRNGGEGYQRVPQNGGGGRGGRGEGGRGGARGGG
                     SS"
     misc_feature    complement(4871..4888)
                     /label=Primer R
     misc_feature    4892..4897
                     /label=enzyme cut site
     promoter        4902..5401
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4937..4960)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5334..5353
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5428..6222
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5482..5501)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6092..6111
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    6246..6834
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6299..6319)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6717..6728)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7042..7675
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7804..7820)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7804..7820)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7817..7839)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7828..7844
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7852..7882)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7897..7918
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8035..8052)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8206..8791)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8286..8305)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8962..9822)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9585..9604
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9823..9927)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    9975..10109
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10012..10031)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10066..10085
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgaggc gttacataac
     2221 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
     2281 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
     2341 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
     2401 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
     2461 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
     2521 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
     2581 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
     2641 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg
     2701 tctatataag cagagctggt ttagtgaacc gtcagatccg ctagcgctac cggtcgccac
     2761 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
     2821 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
     2881 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
     2941 cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa
     3001 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
     3061 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
     3121 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
     3181 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa
     3241 cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc
     3301 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
     3361 ctacctgagc acccagtcca agctgagcaa agaccccaac gagaagcgcg atcacatggt
     3421 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagtc
     3481 cggactcaga tctcgagctc aagcttccat ggcacagcag gaagccagtc cgagccctgg
     3541 tgcagaagtt gtgggccgcg catttgttga acagtattat catattctgc accagagccc
     3601 tggtctggtg catcgttttt atcaggatag cagttttctg acccgtccgg atgttaccgg
     3661 tgccgttacc accgtgacca ccatgcaggc aattaatgat aaaattctga gtctgaagta
     3721 cgaagattat accgcagaaa ttgaaaccgc cgatgcccag gaaagtcatg aacgtggtgt
     3781 tattgtgctg gttaccggtc gcctgaccgg taatgataat gtgcgcaaaa aattttcaca
     3841 gagtttcttt ctggcaccgc aggataaagg ttattttgtg ctgaatgatg ttttccgctt
     3901 tctggaagaa aaagaagtga ccgcacaggc ccgcagcgtt ccgattaatg gcaccacccg
     3961 tgatgttcag gcaccgattg aaccggaacg cgtggtggtt agccatgaac cggaagtgga
     4021 accggaaccg gttgcaagca ttgaagaaga agatctggat aatgtggccg aagtttatga
     4081 tccgagcgat aaagatgaag gtgtggtggt tgatgtggaa ccgattgaac ctccgaccca
     4141 gattagtcat aatgaaattc tgagcgtgcc gcagggcgat gcaccgaaac atagttatgc
     4201 aagtattctg aaacagatga aaagcagccc ggccccgacc acccatgttg cacgcaataa
     4261 gccgcgtccg gcaccggtga atcagaaact gaccgccccg ccggcagaac cggcagcaag
     4321 acctgaagcc agtgcccatg aaaatgttcc gaatagcagc catgtggatg ttgaagatga
     4381 tggtcatagc atctatgttc gcaatctgcc gtttgatagc accccgaccc agctggaaga
     4441 agtgtttaaa aattttggtg caatcaagca tgagggtatt caggttcgca gtaataagca
     4501 gcagggcttt tgttttggtt ttgtggaatt tgaaaccagt agtggtaaac agagtgcact
     4561 ggaagcaagc ccggttacca ttggtgaccg tcaggccgtt gtggaagaaa agaaaaccaa
     4621 tagtcgtggt ggtggcaata atggcggtag ccgtggtcgt tattttagcg gccgtggcag
     4681 ctttcgcaat gaaagtttta aaggcggtcg tggcggcggc ggccgtggtg gttatggtcg
     4741 tggtggcggc gaattttctg gccgtccgaa aagtagcaat ccgcgtaatg gtggcgaagg
     4801 ttatcagcgc gtgccgcaga atggtggtgg tggtcgcggc ggccgtggcg agggtggtcg
     4861 tggtggtgct cgcggtggcg gtagcagcta agaattctac cgggtagggg aggcgctttt
     4921 cccaaggcag tctggagcat gcgctttagc agccccgctg ggcacttggc gctacacaag
     4981 tggcctctgg cctcgcacac attccacatc caccggtagg cgccaaccgg ctccgttctt
     5041 tggtggcccc ttcgcgccac cttctactcc tcccctagtc aggaagttcc cccccgcccc
     5101 gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca
     5161 gatggacagc accgctgagc aatggaagcg ggtaggcctt tggggcagcg gccaatagca
     5221 gctttgctcc ttcgctttct gggctcagag gctgggaagg ggtgggtccg ggggcgggct
     5281 caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg
     5341 cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct tcctcatctc cgggcctttc
     5401 gacctgcagc ccaagcttcc taggaccatg attgaacaag atggattgca cgcaggttct
     5461 ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc
     5521 tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc
     5581 gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc gtggctggcc
     5641 acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg
     5701 ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag
     5761 aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc
     5821 ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt
     5881 cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc
     5941 gccaggctca aggcgagcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc
     6001 tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg
     6061 ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag
     6121 cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg
     6181 cagcgcatcg ccttctatcg ccttcttgac gagttcttct gacccgggcg gggcgcgtct
     6241 ggaacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     6301 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     6361 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     6421 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     6481 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     6541 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     6601 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc
     6661 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     6721 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     6781 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggaa
     6841 ttaattctgc agtcgagacc tagaaaaaca tggagcaatc acaagtagca atacagcagc
     6901 taccaatgct gattgtgcct ggctagaagc acaagaggag gaggaggtgg gtttttccag
     6961 tcacacctca ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact
     7021 ttttaaaaga aaagagggga ctggaagggc taattcactc ccaacgaaga caagatatcc
     7081 ttgatctgtg gatctaccac acacaaggct acttccctga ttagcagaac tacacaccag
     7141 ggccaggggt cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc
     7201 cagataaggt agaagaggcc aataaaggag agaacaccag cttgttacac cctgtgagcc
     7261 tgcatgggat ggatgacccg gagagagaag tgttagagtg gaggtttgac agccgcctag
     7321 catttcatca cgtggcccga gagctgcatc cggagtactt caagaactgc tgatatcgag
     7381 cttgctacaa gggactttcc gctggggact ttccagggag gcgtggcctg ggcgggactg
     7441 gggagtggcg agccctcaga tcctgcatat aagcagctgc tttttgcctg tactgggtct
     7501 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     7561 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     7621 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt
     7681 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt
     7741 gagaggcctt gacattgcta gcgttttacc gtcgacctct agctagagct tggcgtaatc
     7801 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     7861 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     7921 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     7981 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     8041 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     8101 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     8161 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     8221 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     8281 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     8341 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     8401 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     8461 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     8521 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     8581 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     8641 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     8701 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtttttttg tttgcaagca
     8761 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     8821 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     8881 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     8941 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     9001 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     9061 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     9121 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     9181 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     9241 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     9301 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     9361 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     9421 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     9481 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     9541 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     9601 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     9661 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     9721 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     9781 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     9841 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     9901 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga
     9961 cggatcggga gatcaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
    10021 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
    10081 tcatcaatgt atcttatcat gtctggatca actggataac tcaagctaac caaaatcatc
    10141 ccaaacttcc caccccatac cctattacca ctgccaatta cctagtggtt tcatttactc
    10201 taaacctgtg attcctctga attattttca ttttaaagaa attgtatttg ttaaatatgt
    10261 actacaaact tagtagtttt taaagaaatt gtatttgtta aatatgtact acaaacttag
    10321 tagt
//