| 质粒名称: | pGEX6P-bk1 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002152 |
| 产品用途: | Prokaryotic expression vector with Prokaryotic expression tags |
| 存储实验室: | Chen Caiyan Lab (陈彩艳实验室) |
|
载体骨架基本信息 |
|
| 载体名称: | pGEX6P |
| 表达系统: | 细菌表达 |
| 启动子: | tac |
| 拷贝数: | 低拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: |
GST
|
| 物种来源: | NA |
| 密码子优化: | 未优化 |
买家导航
pGEX6P-bk1载体质粒基本信息
pGEX6P-bk1质粒图谱载体图谱和pGEX6P-bk1载体序列质粒序列多克隆位点信息
pGEX6P-bk1质粒载体简介
pGEX6P-bk1质粒序列载体序列
LOCUS pGEX6P-bk1 5017 bp DNA circular SYN 03-APR-2026
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5017)
AUTHORS 123
TITLE Direct Submission
JOURNAL Exported Apr 20, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5017
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 183..211
/label=tac promoter
/note="strong E. coli promoter; hybrid between the trp and
lac UV5 promoters"
protein_bind 219..235
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
CDS 258..911
/codon_start=1
/product="glutathione S-transferase from Schistosoma
japonicum"
/label=GST
/translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
CDS 918..941
/codon_start=1
/product="recognition and cleavage site for human
rhinovirus 3C and PreScission proteases"
/label=HRV 3C site
/translation="LEVLFQGP"
misc_feature 945..986
/label=001
promoter 1320..1424
/gene="bla"
/label=AmpR promoter
CDS 1425..2285
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 2456..3044
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
promoter 3288..3365
/gene="lacI"
/label=lacI promoter
CDS 3366..4448
/codon_start=1
/gene="lacI"
/product="lac repressor"
/label=lacI
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
/translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
ALADSLMQLARQVSRLESGQ"
promoter 4497..4527
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 4535..4551
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 4559..4575
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(4591..4607)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
ORIGIN
1 agcttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg
61 gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt
121 tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc
181 tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca
241 cacaggaaac agtattcatg tcccctatac taggttattg gaaaattaag ggccttgtgc
301 aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc
361 gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc
421 ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata
481 tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc
541 ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact
601 ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag
661 atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt
721 tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa
781 aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat
841 ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc
901 atcctccaaa atcggatctg gaagttctgt tccaggggcc cctgcaggtc gactctcgag
961 gatccggtac ccggtatcga tttaaagaat tcccgggtcg actcgagcgg ccgcatcgtg
1021 actgactgac gatctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc
1081 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc
1141 agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg
1201 atagcggagt gtataattct tgaagacgaa agggcctcgt gatacgccta tttttatagg
1261 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
1321 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
1381 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
1441 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
1501 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
1561 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
1621 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc
1681 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
1741 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
1801 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
1861 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
1921 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa
1981 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
2041 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
2101 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
2161 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
2221 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
2281 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
2341 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
2401 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
2461 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
2521 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
2581 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
2641 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
2701 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
2761 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
2821 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
2881 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
2941 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
3001 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
3061 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
3121 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
3181 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt
3241 attttctcct tacgcatctg tgcggtattt cacaccgcat aaattccgac accatcgaat
3301 ggcgcaaaac ctttcgcggt atggcatgat agcgcccgga agagagtcaa ttcagggtgg
3361 tgaatgtgaa accagtaacg ttatacgatg tcgcagagta tgccggtgtc tcttatcaga
3421 ccgtttcccg cgtggtgaac caggccagcc acgtttctgc gaaaacgcgg gaaaaagtgg
3481 aagcggcgat ggcggagctg aattacattc ccaaccgcgt ggcacaacaa ctggcgggca
3541 aacagtcgtt gctgattggc gttgccacct ccagtctggc cctgcacgcg ccgtcgcaaa
3601 ttgtcgcggc gattaaatct cgcgccgatc aactgggtgc cagcgtggtg gtgtcgatgg
3661 tagaacgaag cggcgtcgaa gcctgtaaag cggcggtgca caatcttctc gcgcaacgcg
3721 tcagtgggct gatcattaac tatccgctgg atgaccagga tgccattgct gtggaagctg
3781 cctgcactaa tgttccggcg ttatttcttg atgtctctga ccagacaccc atcaacagta
3841 ttattttctc ccatgaagac ggtacgcgac tgggcgtgga gcatctggtc gcattgggtc
3901 accagcaaat cgcgctgtta gcgggcccat taagttctgt ctcggcgcgt ctgcgtctgg
3961 ctggctggca taaatatctc actcgcaatc aaattcagcc gatagcggaa cgggaaggcg
4021 actggagtgc catgtccggt tttcaacaaa ccatgcaaat gctgaatgag ggcatcgttc
4081 ccactgcgat gctggttgcc aacgatcaga tggcgctggg cgcaatgcgc gccattaccg
4141 agtccgggct gcgcgttggt gcggatatct cggtagtggg atacgacgat accgaagaca
4201 gctcatgtta tatcccgccg ttaaccacca tcaaacagga ttttcgcctg ctggggcaaa
4261 ccagcgtgga ccgcttgctg caactctctc agggccaggc ggtgaagggc aatcagctgt
4321 tgcccgtctc actggtgaaa agaaaaacca cgctagcgcc caatacgcaa accgcctctc
4381 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
4441 ggcagtgagc gcaacgcaat taatgtaagt tagctcactc attaggcacc ccaggcttta
4501 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
4561 ggaaacagct atgaccatga ttacggattc actggccgtc gttttacaac gtcgtgactg
4621 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
4681 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
4741 cgaatggcgc tttgcctggt ttccggcacc agaagcggtg ccggaaagct ggctggagtg
4801 cgatcttcct gaggccgata ctgtcgtcgt cccctcaaac tggcagatgc acggttacga
4861 tgcgcccatc tacaccaacg tgacctatcc cattacggtc aatccgccgt ttgttcccac
4921 ggagaatccg acgggttgtt actcgctcac atttaatgtt gatgaaagct ggctacagga
4981 aggccagacg cgaattattt ttgatggcgt tggaatt
//
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