| 质粒名称: | pLVX-EF1alpha-SARS-CoV-2-E-2xStrep-IRES-Puro |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 141385 |
| 存储实验室: | Nevan Krogan |
|
载体骨架基本信息 |
|
| 载体名称: | pLVX-EF1alpha-IRES-Puro |
| 载体出品公司: | Takara Bio |
| 空载体大小: | 8825 bp |
| 完整质粒大小: | 9138 bp |
| 载体类型: | 哺乳细胞表达, Lentiviral |
| 筛选标记: | Puromycin |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 30 ℃ |
| 宿主菌: | NEB Stable |
| 拷贝数: | 未知 |
|
插入基因信息 |
|
| 插入基因名称: | SARS-CoV-2-E |
| 物种来源: | SARS-CoV-2 |
| 基因突变: | human codon optimized |
| 启动子: | EF1alpha |
| 标签/融合蛋白: | 2xStrep (C terminal on insert) |
| 克隆方法: | 未知 |
|
文献引用方法 |
|
| 材料和方法部分: |
pLVX-EF1alpha-SARS-CoV-2-E-2xStrep-IRES-Puro was a gift from Nevan Krogan (Addgene plasmid # 141385 ; http://n2t.net/addgene:141385 ; RRID:Addgene_141385)
For viral preps, please replace (Addgene plasmid # 141385) in the above sentence with: (Addgene viral prep # 141385-LV)
|
| 参考文献部分: | A SARS-CoV-2 protein interaction map reveals targets for drug repurposing. Gordon DE, Jang GM, Bouhaddou M, Xu J, Obernier K, White KM, O'Meara MJ, Rezelj VV, Guo JZ, Swaney DL, Tummino TA, Huttenhain R, Kaake RM, Richards AL, Tutuncuoglu B, Foussard H, Batra J, Haas K, Modak M, Kim M, Haas P, Polacco BJ, Braberg H, Fabius JM, Eckhardt M, Soucheray M, Bennett MJ, Cakir M, McGregor MJ, Li Q, Meyer B, Roesch F, Vallet T, Mac Kain A, Miorin L, Moreno E, Naing ZZC, Zhou Y, Peng S, Shi Y, Zhang Z, Shen W, Kirby IT, Melnyk JE, Chorba JS, Lou K, Dai SA, Barrio-Hernandez I, Memon D, Hernandez-Armenta C, Lyu J, Mathy CJP, Perica T, Pilla KB, Ganesan SJ, Saltzberg DJ, Rakesh R, Liu X, Rosenthal SB, Calviello L, Venkataramanan S, Liboy-Lugo J, Lin Y, Huang XP, Liu Y, Wankowicz SA, Bohn M, Safari M, Ugur FS, Koh C, Savar NS, Tran QD, Shengjuler D, Fletcher SJ, O'Neal MC, Cai Y, Chang JCJ, Broadhurst DJ, Klippsten S, Sharp PP, Wenzell NA, Kuzuoglu-Ozturk D, Wang HY, Trenker R, Young JM, Cavero DA, Hiatt J, Roth TL, Rathore U, Subramanian A, Noack J, Hubert M, Stroud RM, Frankel AD, Rosenberg OS, Verba KA, Agard DA, Ott M, Emerman M, Jura N, von Zastrow M, Verdin E, Ashworth A, Schwartz O, d'Enfert C, Mukherjee S, Jacobson M, Malik HS, Fujimori DG, Ideker T, Craik CS, Floor SN, Fraser JS, Gross JD, Sali A, Roth BL, Ruggero D, Taunton J, Kortemme T, Beltrao P, Vignuzzi M, Garcia-Sastre A, Shokat KM, Shoichet BK, Krogan NJ. Nature. 2020 Jul;583(7816):459-468. doi: 10.1038/s41586-020-2286-9. Epub 2020 Apr 30. 10.1038/s41586-020-2286-9 PubMed 32353859 |
买家导航
pLVX-EF1alpha-SARS-CoV-2-E-2xStrep-IRES-Puro载体质粒基本信息
pLVX-EF1alpha-SARS-CoV-2-E-2xStrep-IRES-Puro质粒图谱载体图谱和pLVX-EF1alpha-SARS-CoV-2-E-2xStrep-IRES-Puro载体序列质粒序列多克隆位点信息
pLVX-EF1alpha-SARS-CoV-2-E-2xStrep-IRES-Puro质粒载体简介
pLVX-EF1alpha-SARS-CoV-2-E-2xStrep-IRES-Puro质粒序列载体序列
LOCUS pLVX-EF1alpha-SARS-CoV-2-E-2xStrep-IRES-Puro 9138 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Lentiviral expression of SARS-CoV-2 envelope (E) protein; transient
expression and generate lentivirus.
ACCESSION .
VERSION .
KEYWORDS pLVX-EF1alpha-SARS-CoV-2-E-2xStrep-IRES-Puro
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9138)
AUTHORS Gordon DE, et al.
TITLE A SARS-CoV-2-Human Protein-Protein Interaction Map Reveals Drug
Targets and Potential Drug Repurposing
JOURNAL BioRxiv March 2020
REFERENCE 2 (bases 1 to 9138)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..9138
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 1..1182
/label=EF-1-alpha promoter
/note="strong constitutive promoter for human elongation
factor EF-1-alpha"
intron 231..1173
/label=EF-1-alpha intron A
/note="intron upstream of the start codon of human
EF-1-alpha"
primer_bind 1130..1150
/label=EF1a-F
/note="Human elongation factor-1a promoter, forward primer"
CDS 1456..1479
/codon_start=1
/product="peptide that binds Strep-Tactin(R), an engineered
form of streptavidin"
/label=Strep-Tag II
/translation="WSHPQFEK"
CDS 1516..1539
/codon_start=1
/product="peptide that binds Strep-Tactin(R), an engineered
form of streptavidin"
/label=Strep-Tag II
/translation="WSHPQFEK"
misc_feature 1551..2124
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
primer_bind complement(1718..1735)
/label=IRES reverse
/note="IRES internal ribosome entry site, reverse primer.
Also called pCDH-rev"
primer_bind 1945..1964
/label=IRES-F
/note="IRES internal ribosome entry site, forward primer"
CDS 2157..2756
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(2157..2176)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 2653..2673
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
misc_feature 2770..3358
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(2823..2843)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(3241..3252)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 3565..4198
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
primer_bind complement(4326..4342)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(4326..4342)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(4339..4361)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 4350..4366
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(4374..4404)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 4419..4440
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(4557..4574)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(4728..5316)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(4808..4827)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(5487..6347)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 6110..6129
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(6348..6452)
/gene="bla"
/label=AmpR promoter
polyA_signal 6500..6634
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(6537..6556)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 6591..6610
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
LTR 6802..7435
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
misc_feature 7482..7607
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 8104..8337
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 8522..8566
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 8829..8944
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1 (lacking the first T)"
ORIGIN
1 gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg
61 gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg
121 atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag
181 tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg
241 tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg ccttgaatta
301 cttccacgcc cctggctgca gtacgtgatt cttgatcccg agcttcgggt tggaagtggg
361 tgggagagtt cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc
421 tggcttgggc gctggggccg ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct
481 gctttcgata agtctctagc catttaaaat ttttgatgac ctgctgcgac gctttttttc
541 tggcaagata gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc ggtttttggg
601 gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg
661 cgagcgcggc caccgagaat cggacggggg tagtctcaag ctggccggcc tgctctggtg
721 cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg caaggctggc ccggtcggca
781 ccagttgcgt gagcggaaag atggccgctt cccggccctg ctgcagggag ctcaaaatgg
841 aggacgcggc gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa aagggccttt
901 ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc caggcacctc
961 gattagttct cgagcttttg gagtacgtcg tctttaggtt ggggggaggg gttttatgcg
1021 atggagtttc cccacactga gtgggtggag actgaagtta ggccagcttg gcacttgatg
1081 taattctcct tggaatttgc cctttttgag tttggatctt ggttcattct caagcctcag
1141 acagtggttc aaagtttttt tcttccattt caggtgtcgt gaggatctat ttccggtgaa
1201 ttcgccgcca ccatgtacag cttcgtatca gaagaaaccg ggacactgat cgtaaattct
1261 gtgctcttgt ttctggcatt cgtcgtattt ctcctcgtca cactggcaat tctgactgca
1321 ttgaggcttt gcgcctactg ttgtaacatt gtcaatgtat ctctcgtgaa accctcattc
1381 tacgtttaca gcagggtgaa gaatctcaat tctagcaggg tgccggatct cctcgttctc
1441 gaaggcggcg ggggatggag ccatccacaa ttcgagaaag gcggtggttc aggaggaggt
1501 agcgggggtg gatcatggtc acatccgcag tttgaaaagt aaggatcccg cccctctccc
1561 tccccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc
1621 tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc
1681 cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt
1741 ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct
1801 gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa
1861 aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt
1921 tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag
1981 gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg cacatgcttt
2041 acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt
2101 ttcctttgaa aaacacgatg ataagcttgc cacaacccac aaggagacga ccttccatga
2161 ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtcccccgg gccgtacgca
2221 ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgac ccggaccgcc
2281 acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg
2341 gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg
2401 tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc
2461 ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg
2521 cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg
2581 ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga
2641 cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg
2701 tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctagacgc
2761 gtctggaaca atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
2821 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
2881 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
2941 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
3001 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
3061 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
3121 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca
3181 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
3241 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
3301 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct
3361 ggaattaatt ctgcagtcga gacctagaaa aacatggagc aatcacaagt agcaatacag
3421 cagctaccaa tgctgattgt gcctggctag aagcacaaga ggaggaggag gtgggttttc
3481 cagtcacacc tcaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
3541 actttttaaa agaaaagagg ggactggaag ggctaattca ctcccaacga agacaagata
3601 tccttgatct gtggatctac cacacacaag gctacttccc tgattagcag aactacacac
3661 cagggccagg ggtcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg
3721 agccagataa ggtagaagag gccaataaag gagagaacac cagcttgtta caccctgtga
3781 gcctgcatgg gatggatgac ccggagagag aagtgttaga gtggaggttt gacagccgcc
3841 tagcatttca tcacgtggcc cgagagctgc atccggagta cttcaagaac tgctgatatc
3901 gagcttgcta caagggactt tccgctgggg actttccagg gaggcgtggc ctgggcggga
3961 ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc ctgtactggg
4021 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
4081 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
4141 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
4201 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag
4261 agtgagaggc cttgacattg ctagcgttta ccgtcgacct ctagctagag cttggcgtaa
4321 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
4381 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
4441 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
4501 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
4561 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
4621 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
4681 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
4741 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
4801 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
4861 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
4921 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
4981 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
5041 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
5101 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
5161 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
5221 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
5281 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
5341 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
5401 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
5461 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
5521 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
5581 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
5641 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
5701 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
5761 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
5821 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
5881 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
5941 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
6001 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
6061 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
6121 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
6181 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
6241 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
6301 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
6361 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
6421 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
6481 gtcgacggat cgggagatca acttgtttat tgcagcttat aatggttaca aataaagcaa
6541 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
6601 caaactcatc aatgtatctt atcatgtctg gatcaactgg ataactcaag ctaaccaaaa
6661 tcatcccaaa cttcccaccc cataccctat taccactgcc aattacctgt ggtttcattt
6721 actctaaacc tgtgattcct ctgaattatt ttcattttaa agaaattgta tttgttaaat
6781 atgtactaca aacttagtag ttggaagggc taattcactc ccaaagaaga caagatatcc
6841 ttgatctgtg gatctaccac acacaaggct acttccctga ttagcagaac tacacaccag
6901 ggccaggggt cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc
6961 cagataaggt agaagaggcc aataaaggag agaacaccag cttgttacac cctgtgagcc
7021 tgcatgggat ggatgacccg gagagagaag tgttagagtg gaggtttgac agccgcctag
7081 catttcatca cgtggcccga gagctgcatc cggagtactt caagaactgc tgatatcgag
7141 cttgctacaa gggactttcc gctggggact ttccagggag gcgtggcctg ggcgggactg
7201 gggagtggcg agccctcaga tcctgcatat aagcagctgc tttttgcctg tactgggtct
7261 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
7321 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
7381 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
7441 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
7501 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
7561 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
7621 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
7681 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
7741 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
7801 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
7861 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
7921 aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg gaggaggaga
7981 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
8041 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
8101 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
8161 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
8221 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
8281 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
8341 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
8401 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
8461 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
8521 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
8581 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
8641 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
8701 attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga
8761 agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg
8821 gtatcgcctt taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag
8881 acataatagc aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa
8941 attttcgggt ttattacagg gacagcagag atccagttta tcgatgagta attcatacaa
9001 aaggactcgc ccctgccttg gggaatccca gggaccgtcg ttaaactccc actaacgtag
9061 aacccagaga tcgctgcgtt cccgccccct cacccgcccg ctctcgtcat cactgaggtg
9121 gagaagagca tgcgtgag
//
